Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,809,905	G→A	Q27Q (CAG→CAA)	coaD →	pantetheine‑phosphate adenylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,809,905	0	G	A	95.7%	144.7 / NA	46	Q27Q (CAG→CAA)	coaD	pantetheine‑phosphate adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (23/21);  minor base C (2/0);  total (25/21)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTATGCAAAAACGGGCGATTTATCCGGGTACTTTCGATCCCATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAGATGTTCGATCACGTTATTCTGGCGATTGCCGCCAGCCCCAGTAAAAAACCGATGTTTACCCTGGAAGAGCGTGTGGCA‑CTGGCACAGCAGGCAACCGCGCATCTGGGGAACGTGGAAGTGGTCGGGTTTAGTGATTTAATGGCGAACTTCGCCCGTAATCAACACGCTACGGTGCTGATTCGTGGCCTGCGTGCGGTGGCAGATTTTGAATATGAAATGCAGCTGGCGCATATGAATCGC  >  NC_000913/3809662‑3810145                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  acgtcGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTATGCAAAAACGGGCGATTTATCCGGGTACTTTCGATCCCATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTTCg                                                                                                                                                                                                                                            <  2:235467/247‑1 (MQ=255)           cAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTATGCAAAAACGGGCGATTTATCCGGGTACTTTCGATCCCATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCACATGTTCGATCACGTTAt                                                                                                                                                                                                                                  >  2:21289/1‑251 (MQ=255)                 agGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTATGCAAAAACGGGCGATTTATCCGGGTACTTTCGATCCCATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTTCGATCACGTTATTCTGGc                                                                                                                                                                                                                            >  1:372524/1‑251 (MQ=255)                  ggTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTATGCAAAAACGGGCGATTTATCCGGGTACTTTCGATCCCATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTTCGATCACGTTATTCTGGCg                                                                                                                                                                                                                           <  2:68380/251‑1 (MQ=255)                         tctTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTATGCAAAAACGGGCGATTTATCCGGGTACTTTCGATCCCATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTTCGATCACGTTATTCTGGCGATTgccg                                                                                                                                                                                                                    >  2:31728/1‑251 (MQ=255)                          ctTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTATGCAAAAACGGGCGATTTATCCGGGTACTTTCGATCCCATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTTCGATCACGTTATTCTGGCGATTgcc                                                                                                                                                                                                                     <  2:113130/249‑1 (MQ=255)                            ttACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTATGCAAAAACGGGCGATTTATCCGGGTACTTTCGATCCCATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTTCGATCACGTTATTCTGGCGATTgccgcc                                                                                                                                                                                                                  <  1:383994/250‑1 (MQ=255)                                 cACCGACGCCGATTACCGTAGTTTCTATGGCCTTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCTTCTGCTTCAACTGCTGTAACCGTACCTGCCACCGAAAACGCATTGAGGTTGTTATGCAAAAACGGGCGATTTATCCGGGTACTTTCGATCCCATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTTCGATCACTTTATTCTGGCGATTGCCGCCAGcccc                                                                                                                                                                                                            <  2:267692/251‑1 (MQ=255)                                   ccGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTATGCAAAAACGGGCGATTTATCCGGGTACTTTCGATCCCATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTTCGATCACGTTATTCTGGCGATTGCCGCCAGcccc                                                                                                                                                                                                            >  2:5670/1‑249 (MQ=255)                                            aTTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTATGCAAAAACGGGCGATTTATCCGGGTACTTTCGATCCCATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTTCGATCACGTTATTCTGGCGATTGCCGCCAGCCCCAGTAAAAAAcc                                                                                                                                                                                                 >  1:6976/1‑251 (MQ=255)                                                     gTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTATGCAAAAACGGGCGATTTATCCGGGTACTTTCGATCCCATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTTCGATCACGTTATTCTGGCGATTGCCGCCAGCCCCAGTAAAAAACCGATGTTTAc                                                                                                                                                                                        <  1:150626/251‑1 (MQ=255)                                                       ttCTCTGGCCGACATGCCGTCGAACTACTGTATCAACCCAAAGGCGCGCTAAAACGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTATGCAACAACGGGCGATTTATCCGGGTCCTTTCGATCCCATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGAACATGTTCGATCACGTTATTCTGGCGATTGCCGCCAGCCCCAGTAAAAAACCGATGTTTAccc                                                                                                                                                                                      >  2:315366/1‑251 (MQ=255)                                                       tacaaTGGCCGTCATGCCGTTGAAGCACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTACGCAAAAACGGGAGATTTATCCGGGTACTTTCGATCCCATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTTCGATCACGTTATTCTGGCGGTTGCCGCCAGCCCCAGTAAACAACCGATGTTTAccc                                                                                                                                                                                      >  2:349178/5‑251 (MQ=255)                                                          tATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTATGCAAAAACGGGCGATTTATCCGGGTACTTTCGATCCCATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTTCGATCACGTTATTCTGGCGATTGCCGCCAGCCCCAGTAAAAAACCGATGTTTACCCTgg                                                                                                                                                                                   >  2:7239/1‑251 (MQ=255)                                                          tATGGCCATCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTATGCAAAAACGGGCGATTTATCCGGGTACTTTCGATCCCATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTTCGATCACGTTATTCTGGCGATTGCCGCCAGCCCCAGTAAAAAACCGATGTTTACCCTgg                                                                                                                                                                                   >  1:318372/1‑251 (MQ=255)                                                           aTGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTATGCAAAAACGGGCGATTTATCCGGGCACTTTCGATCCCATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTTCGATCACGTTATTCTGGCGATTGCCGCCAGCCCCAGTAAAAAACCGATGTTTACCCTgg                                                                                                                                                                                   >  1:177068/1‑250 (MQ=255)                                                                      gCCGTTGAATTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTATGCAAAAACGGGCGATTTATCCGGGTACTTTCGATCCCATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTTCGATCACGTTATTCTGGCGATTGCCGCCAGCCCCAGTAAAAAACCGATGTTTACCCTGGAAGAGCGTGtgg                                                                                                                                                                       <  1:420499/251‑1 (MQ=255)                                                                            gAAGTACTGTATCAAAACCAGAGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTATGCAAAAACGGGCGATTTATCCGGGTACTTTCGATCCCATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTTCGATCACGTTATTCTGGCGATTGCCGCCAGCCCCAGTAAAAAACCGATGTTTACCCTGGCAGAGCGTGtggagcttg                                                                                                                                                                 >  2:438256/1‑245 (MQ=255)                                                                             aaGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTATGCAAAAACGGGCGATTTATCCGGGTACTTTCGATCCCATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTTCGATCACGTTATTCTGGCGATTGCCGCCAGCCCCAGTAAAAAACCGATGTTTACCCTGGAAGAGCGTGtggagctggc                                                                                                                                                                >  1:203508/1‑244 (MQ=255)                                                                               gTACTGTCTCAAAACCAGGGCGCGCTAAAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTATGCAAAAACGGGCGATTTATCCGGGTACTTTCGATCCCATTACAAATGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTTCGATCACGTTATTCTGGCGCTTGCCGCCAGCCCCAGTAAAAAACCGATGTTTACACTGGAAGAGCGTGtggat‑ctggcac                                                                                                                                                             >  2:191202/1‑251 (MQ=255)                                                                                              cAGGGCGCGCTACAGCGTATGCTTCAACTGCTGGAACCTTCCCTGCCACCGACAACGCATTGAGGTTGTTATGCAAAAACGGGCGATTTATCCGGGTACTTTCGATCCCATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTTCGATCACGTTATTCTGCCGATTGCCGCCAGCCCCAGTAAAAAACCGATGTTTACCCTGGAAGAGCGTGTGGAG‑CTGGCACAGCAGGCAACCgcgc                                                                                                                                              >  2:83334/1‑251 (MQ=255)                                                                                                         aCAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTATGCAAAAACGGGCGATTTATCCGGGTACTTTCGAGCCCATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTGCGATCACGTTATTCTGGCGAGTGCCGCCAGCCCCAGGAAAAAACCGATTTTTGCCCTGGAAGAGCGTGTGGAG‑CTGGCACAGCAGGCAGCCGCGCATCTGGGGaa                                                                                                                                    <  2:437273/250‑1 (MQ=255)                                                                                                                  gCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTATGCAAAAACGGGCGATTTATCCGGGTACTTTCGATCCCATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTTCGATCACGTTATTCTGGCGATTGCCGCCAGCCCCAGTAAAAAACCGATGTTTACCCTGGAAGAGCGTGTGGAG‑CTGGCACAGCAGGCAACCGCGCATCTGGGGAACGTGGAAGTg                                                                                                                          <  2:372524/251‑1 (MQ=255)                                                                                                                  gCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTATGCAAAAACGGGCGATTTAGCCGGGTACTTTCGATCCCATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTTCGATCACGTTATTCTGGCGATTGCCGCCAGCCCCAGTAAAAAACCGAGGTGTACCCTGGAAGAGCGTGTGGAG‑CTGGCACAGCAGGCAACCGCGCATCTGGGGAACGTGGaa                                                                                                                             <  2:259727/248‑1 (MQ=255)                                                                                                                       aaCTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTATGCAAAAACGGGCGATTTATCCGGGTACTTTCGATCCCATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTTCGATCACGTTATTCTGGCGATTGCCGCCAGCCCCAGTAAAAAACCGATGTTTACCCTGGAAGAGCGTGTGGAG‑CTGGCACAGCAGGCAACCGCGCATCTGGGGAACGTGGAAGTGGTCgg                                                                                                                     >  1:67947/1‑251 (MQ=255)                                                                                                                              ggAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTATGCAAAAACGGGCGATTTATCCGGGTACTTTCGATCCCATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTTCGATCACGTTATTCTGGCGATTGCCGCCAGCCCCAGTAAAAAACCGATGTTTACCCTGGAAGAGCGTGTGGAG‑CTGGCACAGCAGGCAACCGCGCATCTGGGGAACGTGGAAGTGGTCGGGTTTAGt                                                                                                              <  2:150687/251‑1 (MQ=255)                                                                                                                                 aCCTTACCTGCCACCGAAAACGCATTGAGGTTGTTATGCAAAAACGGGCGATTTATCCGGGTACTTTCGCTCCCATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTTCGATCACGTTATTCTGGCGATTGCCGCCAGCCCCAGTAAAAAACCGATGTTTACCCTGGCAGAGCGTGTGGAG‑CTGGCACAGCAGGCAACCGCGCATCTGGGGAACGTGGAAGGGGTCGGGTTTAGTGAt                                                                                                           >  1:260703/1‑251 (MQ=255)                                                                                                                                      aCCTGCCACCGAAAACGCATTGAGGTTGTTATGCAAAAACGGGCGATTTATCCGGGTACTTTCGATCCCATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTTCGATCACGTTATTCTGGCGATTGCCGCCAGCCCCAGTAAAAAACCGATGTTTACCCTGGAAGAGCGTGTGGAG‑CTGGCACAGCAGGCAACCGCGCATCTGGGGAACGTGGAAGTGGTCTGGTTTAGTGAtttgat                                                                                                      <  1:305750/251‑4 (MQ=255)                                                                                                                                          gCCACCGAAAACGCATTGAGGTTGTTATGCAAAAACGGGCGATTTATCCGGGTACTTTCGATCCCATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTTCGATCACGTTATTCTGGCGATTGCCGTCAGCCCCAGTAAAAAACCGATGTTTACCCTGGAAGAGCGTGTGGAG‑CTGGCACAGCAGGCAACCGCGCATCTGGGGAACGTGGAAGTGGTCGGGTTTAGTGATTTGATGGc                                                                                                   <  1:393255/250‑1 (MQ=255)                                                                                                                                             aCCGAAAACGCATTGAGGTTGTTATGCAAAAACGGGCGATTTATCCGGGTACTTTCGCTCCCATTACCAATGGTCATATCGATATAGTGACGCACGCCACGAAAATGTTCGATCACGTTATTCTGGCGATTGCCGCCAGCCGCAGTAAACAACCGATGTTTACCCTGGAGGAGCGTGTG‑CAGCTGGCACAGCAGGCAACCGCGCATCTGGGGAACGTGGAAGGGGTCGGGTTTatgggtttgatagcgaac                                                                                               >  1:71956/1‑234 (MQ=255)                                                                                                                                             aCCGAAAACGCATTGAGGTTGTTATGCAAAAACGGGCGATTTATCCGGGTACTTTCGATCCCATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTTCGATCACGTTATTCTGGCGATTGCCGCCAGCCCCAGTAAAAAACCGATGTTTACCCTGGAAGAGCGTGTGGAG‑CTGGCACAGCAGGCAACCGCGCATCTGGGGAACGTGGAAGTGGTCGGGTTTAGTGATTTGATGGCGAAc                                                                                               >  1:15166/1‑251 (MQ=255)                                                                                                                                                gAAAACGCATTGAGGTTGTTATGCAAAAACGGGCGATTTATCCGGGTACTTTCGATCCCATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTTCGATCACGTTATTCTGGCGATTGCCGCCAGCCCCAGTAAAAAACCGATGTTTACCCTGGAAGAGCGTGTGGAG‑CTGGCACAGCAGGCAACCGCGCATCTGGGGAACGTGGAAGTGGTCGGGTTTAGTGATTTGATGGCGAACTTc                                                                                            <  2:303161/251‑1 (MQ=255)                                                                                                                                                   aaCGCATTGAGGTTGTTATGCAAAAACGGGCGATTTATCCGGGTACTTTCGATCCCATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTTCGATCACGTTATTCTGGCGATTGCCGCCAGCCCCAGTAAAAAACCGATGTTTACCCTGGAAGAGCGTGTGGAG‑CTGGCACAGCAGGCAACCGCGCATCTGGGGAACGTGGAAGTGGTCGGGTTTAGTGATTTGATGGCGAACTTCGcc                                                                                         >  1:290297/1‑251 (MQ=255)                                                                                                                                                    aCGCATTGAGGTTGTTATGCAAAAACGGGCGATTTATCCGGGTACTTTCGATCCCATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTTCGATCACGTTATTCTGGCGATTGCCGCCAGCCCCAGTAAAAAACCGATGTTTACCCTGGAAGAGCGTGTGGAG‑CTGGCACAGCAGGCAACCGCGCATCTGGGGAACGTGGAAGTGGTCGGGTTTAGTGATTTGATGGCGAACTTCGccc                                                                                        <  1:159404/251‑1 (MQ=255)                                                                                                                                                           gAGGTTGTTATGCAAAAACGGGCGATTTATCCGGGTACTTTCGATCCCATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTTCGATCACGTTATTCTGGCGATTGCCGCCAGCCCCAGTAAAAAACCGATGTTTACCCTGGAAGAGCGTGTGGAG‑CTGGCACAGCAGGCAACCGCGCATCTGGGGAACGTGGAAGTGGTCGGGTTTAGTGATTTGATGGCGAACTTCGCCCGTAATc                                                                                  <  1:204809/250‑1 (MQ=255)                                                                                                                                                             ggTTGTTATGCAAAAACGGGCGATTTATCCGGGTACTTTCGATCCCATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTTCGATCACGTTATTCTGGCGATTGCCGCCAGCCCCAGTAAAAAACCGATGTTTACCCTGGAAGAGCGTGTGGAG‑CTGGCACAGCAGGCAACCGCGCATCTGGGGAACGTGGAAGTGGTCGGGTTTAGTGATTTGATGGCGAACTTCGCCCGTAATCAac                                                                               >  2:416457/1‑251 (MQ=255)                                                                                                                                                                                         ccGGGTACTTTCGATCCCATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTTCGATCACGTTATTCTGGCGCTTGCCGCCAGCCCCAGTAAAAAACCGATGTTTACCCTGGAAGAGCATGTGGAG‑CTGGCACAGCAGGCAACCGCGCCTCTGGGGAACGTGGAAGTGGTCGGGTTTAGTGATTTGATGGCGAACTTCGCCCGTAATCAACACGCTACGGTGCTGATTCGTGGCCtgcg                                                   >  1:432616/1‑251 (MQ=255)                                                                                                                                                                                             gTACTTTCGATCCCATTACCACTGGTCATATCGATATCGTGACGCGCGCCACGAAAATGTTCGATCACGTTATTCTGGCGATTGCCGACAGCCCCAGTAAAAAACCGATGTTTCCCCTGGAAGAGCGTGTGGAG‑CTGGCACAGCAGGCAACCGCGCATCTGGGGAACGTGGAAGTGGTCGGGTTTAGTGATTTGATGGCGAACTTCGCCCGTAATCAACACGCTACGGTGCTGATTCGTGGCCtgcgggcg                                               >  2:428388/1‑251 (MQ=255)                                                                                                                                                                                                        cccATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTTCGATCACGTTATTCTGGCGATTGCCGCCAGCCCCAGTAAAAAACCGATGTTTACCCTGGAAGAGCGTGTGGAG‑CTGGCACAGCAGGCAACCGCGCATCTGGGGAACGTGGAAGTGGTCGGGTTTAGTGATTTGATGGCGAACTTCGCCCGTAATCAACACGCTACGGTGCTGATTCGTGGCCTGCGTGCGGTGGCGGAttt                                    <  1:31728/251‑1 (MQ=255)                                                                                                                                                                                                        cccATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTTCGATCACGTTATTCTGGCGATTGCCGCCAGCCCCAGTAAAAAACCGATGTTGACCCTGGAAGAGCGTGTGGAG‑CTGGCACAGCAGGCAACCGCGCATCTGTGTAACGTGGAAGTGGTCGGGTTTAGTGATTTGATGGCGAACTTCGCCCGTAATCAACACGCTACGGTGCTGATTCGTGGCCTGCGTGCGGTGGCGGAtt                                     <  1:248234/250‑1 (MQ=255)                                                                                                                                                                                                           aTTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTTCGATCACGTTATTCTGGCGATTGCCGCCAGCCCCAGTAAAAAACCGATGTTTACCCTGGAAGAGCGTGTGGAG‑CTGGCACAGCAGGCAACCGCGCATCTGGGGAACGTGGAAGTGGTCGGGTTTAGTGATTTGATGGCGAACTTCGCCCGTAATCAACACGCTACGGTGCTGATTCg                                                            >  2:214643/1‑224 (MQ=255)                                                                                                                                                                                                           aTTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTTCGATCACGTTATTCTGGCGATTGCCGCCAGCCCCAGTAAAAAACCGATGTTTACCCTGGAAGAGCGTGTGGAG‑CTGGCACAGCAGGCAACCGCGCATCTGGGGAACGTGGAAGTGGTCGGGTTTAGTGATTTGATGGCGAACTTCGCCCGTAATCAACACGCTACGGTGCTGATTCg                                                            <  1:214643/224‑1 (MQ=255)                                                                                                                                                                                                                  aTGGTCATATCGATATCGTGACGCGCGCCACGCAAATGTTCGATCACGTTATTCTGGCGATTGCCGCCAGCCCCAGTAAAAAACCGATGTTTACCCTGGAAGAGCGTGTGGAG‑CTGGCACAGCAGGCAACCGCGCATCTGGGGAACGTGGAAGTGGTCGGGTTTAGTGATTTGATGGCGAACTTCGCCCGTAATCAACACGCTACGGTGCTGATTCGTGGCCTGCGTGCGGTGGCGGATTTTGAATATGa                           >  2:253830/1‑250 (MQ=255)                                                                                                                                                                                                                      tCATATCGATATCGTGACGCGCGCCACGCAAATGTTCGATCACGTTATTCTGGCGATTGCCGCCAGCCCCAGTAAAAAACCGATGTTTACCCTGGAAGAGCGTGTGGAG‑CTGGCACAGCAGGCAACCGCGCATCTGGGGAACGTGGAAGTGGTCGGGTTTAGTGATTTGATGGCGAACTTCGCCCGTAATCAACACGCTACGGTGCTGATTCGTGGCCTGGGTGCGGTGGCGGATTTTGAATATGAAATg                       >  2:93403/1‑250 (MQ=255)                                                                                                                                                                                                                                      aCGCGCGCCACGCAAATGTTCGATCACGTTATTCTGTCGATTGCCGCCAGCCCCAGTAAAAAACCGATGTTTTCCCTGGAAGAGCGTGTGGAG‑CTGGCACAGCAGGCAACCGCGCATCTGGGGAACGTGGAAGTGGTCGGGTTTAGTGAGTTGATGGCGAACTTCGCCCGTAATCAACACGCTACGGTGCTGATTCGTGGCCTGCGTGCGGTGGCGGATTTTGAATATGAAATGCAGCTGGCGCATATGaa      <  1:321214/251‑1 (MQ=255)                                                                                                                                                                                                                                          gcgcCACGCAAATGTTCGATCACGTTATTCTGGCGATTGCCGCCAGCCCCAGTAAAAAACCGATGTTTACCCTGGAAGAGCGTGTGGAG‑CTGGCACAGCAGGCAACCGCGCATCTGGGGAACGTGGAAGTGGTCGGGTTTAGTGATTTGATTGCGAACTTCGCCCGTAATCAACACGCTACGGTGCTGATTCGTGGCCTGCGTGCTGTGGCGGATTTTGAATATGAAATGCAGCGGGCGCATATGAATCGc  <  1:358669/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  ACTACGCTTGCAAAAGAGGTTTCCTCTTTACTCACCGACGCCGATTACCGTAGTTTCTATGGCCGTCATGCCGTTGAAGTACTGTATCAAAACCAGGGCGCGCTACAGCGTCTGCTTCAACTGCTGGAACCTTACCTGCCACCGAAAACGCATTGAGGTTGTTATGCAAAAACGGGCGATTTATCCGGGTACTTTCGATCCCATTACCAATGGTCATATCGATATCGTGACGCGCGCCACGCAGATGTTCGATCACGTTATTCTGGCGATTGCCGCCAGCCCCAGTAAAAAACCGATGTTTACCCTGGAAGAGCGTGTGGCA‑CTGGCACAGCAGGCAACCGCGCATCTGGGGAACGTGGAAGTGGTCGGGTTTAGTGATTTAATGGCGAACTTCGCCCGTAATCAACACGCTACGGTGCTGATTCGTGGCCTGCGTGCGGTGGCAGATTTTGAATATGAAATGCAGCTGGCGCATATGAATCGC  >  NC_000913/3809662‑3810145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑