Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,809,352	G→A	P271P (CCG→CCA)	waaA →	KDO transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,809,352	0	G	A	100.0%	215.1 / NA	63	P271P (CCG→CCA)	waaA	KDO transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/29);  total (34/29)

TTTGTGGCGCTGGGCGCAAAAAATAATCAGGTGACCGTTACCGGTAGCCTGAAATTCGATATTTCTGTAACGCCGCAGTTGGCTGCTAAAGCCGTGACGCTGCGCCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATGGCATTGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATA  >  NC_000913/3809110‑3809593                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  tttGTGGCGCTGGGCGCAAAAAATAACCAGGTAACCGTTACCGGTAGCCTGAAATTCGATATTTCTGTAACGCCGCAGTTGGCGGCTAAAGCCGTGACGCTGCGCCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATGGCTCATCCTGGTACCCCGTCATCCAGAACGCt                                                                                                                                                                                                                                            <  2:51035/250‑1 (MQ=255)      ggCGCTGGGCGCAAAAAATAACCAGGTAACCGTTACCGGTAGCCTGAAATTCGATATTTCTGTAACGCCGCAGTTGGCTGCTAAAGCCGTGACGCTGCGCCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCgg                                                                                                                                                                                                                                      >  1:348174/1‑251 (MQ=255)      ggCGCTGGGCGCAAAAAATAACCAGGTAACCGTTACCGGTAGCCTGAAATTCGATATTTCTGTAACGCCGCAGTTGGCTGCTAAAGCCGTGACGCTGCGCCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCg                                                                                                                                                                                                                                       <  1:130821/250‑1 (MQ=255)      ggCGCTGGGCCCAAAAAATAACCAGGTAACCGTTACCTGTAGCCTGAAATTCGATATTTCTGTAACGCCGCAGTTGGCTGCTAAAGCCGTGACGCTGCGCCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGATTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAAGTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCgt                                                                                                                                                                                                                                      <  2:240387/251‑2 (MQ=255)      ggCGCTGGGAGCAAAAAATAACCAGGTAACCGTTACCGGTAGCCTGAAATTCGATATTTCTGTAACGCCGCAGTTGGCTGCTAAAGCCGTGACGCTGCGCCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCgg                                                                                                                                                                                                                                      <  2:207185/251‑1 (MQ=255)          cTGGGCGCAAAAAATAACCAGGTAACCGTTACCGGTAGCCTGAAATTCGATATTTCTGTAACGCCGCAGTTGGCTGCTAAAGCCGTGACGCTGCGCCGCCAGTGGGCACCACACCGACCGGTATGGATTGCCACCAGCACTCACGAAGACGAAGAGTGTGTGGTGATCGCAGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTACCGGATGc                                                                                                                                                                                                                                  >  1:214017/1‑251 (MQ=255)                   aaaaaTAACCAGGTAACCGTTACCGGTAGCCTGAAATTCGATATTTCTGTAACGCCGCAGTTGGCTGCTAAAGCCGTGACGCTGCGCCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTaatct                                                                                                                                                                                                                         >  2:424813/1‑248 (MQ=255)                              gttaaCCGTTACCGGTAGCCTGAAATTCGATATTTCTGTAACGCCGCAGTTGGCTGCTAAAGCCGTGACGCTGCGCCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGGCATCCAGAACGCGTCCCGGATGCGATTAAGCTTGTCCGCCAgg                                                                                                                                                                                                              <  2:70360/247‑1 (MQ=255)                              ggtaaCCGTTACCGGTAGCCTGAAATTCGATATTTCTGTAACGCCGCAGTTGGCTGCTAAAGCCGTGACGCTGCGCCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAgg                                                                                                                                                                                                              <  1:122616/247‑1 (MQ=255)                                taaCCGTTACCGGTAGCCTGAAATTCGATATTTCTGTAACGCCGCAGTTGGCTGCTAAAGCCGTGACGCTGCGCCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCt                                                                                                                                                                                                            >  2:331572/3‑251 (MQ=255)                                   ccGTTACCGGTAGCCTGAAATTCGATATTTCTGTAACGCCGCAGTTGGCTGCTAAAGCCGTGACGCTGCGCCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGACGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTgg                                                                                                                                                                                                          >  1:256107/1‑250 (MQ=255)                                        aCCGGTAGCCTGAAATTCGATATTTCTGGAACGCCGCAGTTGGCTGCTAAAGCCGTGACGCTGCGCCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGTTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTaat                                                                                                                                                                                                    <  2:243210/251‑2 (MQ=255)                                        aCCGGTAGCCTGAAATTCGATATTGCTGTAACGCCGCAGTTGGCTGCTAAAGCCGTGACGCTGCGCCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAAGCTTGTCCGCCAGGCTGGACTaa                                                                                                                                                                                                     <  1:161967/250‑1 (MQ=255)                                          cGGTAGCCTGAAATTCGATATTTCTGTAACGCCGCAGTTGGCTGCTAAAGCCGTGACGCTGCGCCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCt                                                                                                                                                                                                  >  1:150687/1‑251 (MQ=255)                                                  tGAAATTCGATATTTCTGTAACGCCGCAGTTGGCTGCTAAAGCCGTGACGCTGCGCCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATcac                                                                                                                                                                                           >  1:268791/1‑250 (MQ=255)                                                   gAAATTCGATATTTCTGTAACGCCGCCGTTGGCTGCTACAGCCGTGACGCTGCGAAGCCCGTGGGCACCACACCGCCCGGTATGGATTGCCGCCAGCACTCACGAAGGCGCAGAGAGTGTGGTGATCGCCGCACATCCGGCATTGTTACAGCACTTCCCGAGTTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGCTGCGATAAGTCTTGCCCGCCAGGCTGGACTAAGCTATATAacac                                                                                                                                                                                         >  2:71778/1‑251 (MQ=255)                                                          gATATTTCTGTAACGCCGCAGTTGGCTGCTAAAGCCGTGACGCTGCGCAGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGACGGCGAAGAGCGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGCACGCTTCCCGGATGCGCTTACTCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTc                                                                                                                                                                                  >  2:383994/1‑251 (MQ=255)                                                                    tAACGCCGCAGTTGGCTGCTAAAGCCGTGACGCTGCGCCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTc                                                                                                                                                                        >  1:144006/1‑251 (MQ=255)                                                                             aGTTGGCTGCTAAAGCCGTGACGCTGCGCCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAg                                                                                                                                                                                >  1:302735/1‑234 (MQ=255)                                                                             aGTTGGCTGCTAAAGCCGTGACGCTGCGCCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAg                                                                                                                                                                                <  2:302735/234‑1 (MQ=255)                                                                                     gctAAAGCCGTGACGCTGCGCCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCa                                                                                                                                                       <  1:416733/251‑1 (MQ=255)                                                                                       tAAAGCCGTGACGCTGCGCCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGACGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTccc                                                                                                                                                                                                                                        >  2:436338/1‑168 (MQ=255)                                                                                       tAAAGCCGTGACGCTGCGCCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTccc                                                                                                                                                                                                                                        <  1:436338/168‑1 (MQ=255)                                                                                        aaaGCCGTGACGCTGCGCCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAgg                                                                                                                                                     <  2:416088/250‑1 (MQ=255)                                                                                                 aCGCTGCGCCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATGGTTACAGCAATTCGCTAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAAGCTTGTCCGCCATGCTGGACTAAGCTATATCACACGCTCTTCAGGTGAAGTGCCCTCCACCAGCACGCAGGTTGTGGtggg                                                                                                                                           <  2:429876/251‑4 (MQ=255)                                                                                                        gccgccACTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGAGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGAGGGCGATACg                                                                                                                                    >  2:172815/1‑251 (MQ=255)                                                                                                           gccAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTGGGGGATACGATg                                                                                                                                 >  2:181337/1‑251 (MQ=255)                                                                                                                   gCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTGGGCGATACGATGGGCGAGtt                                                                                                                         <  2:268791/251‑1 (MQ=255)                                                                                                                                 ggTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTGGGCGATACGATGGGCGAGTTGATGTTACTGTacg                                                                                                           >  1:179341/1‑249 (MQ=255)                                                                                                                                  gTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTGGGCGATACGATGGGCGAGTTGATGTTACTGTacgg                                                                                                          <  1:305821/251‑4 (MQ=255)                                                                                                                                   tATGGATTGCCACCAGCACTCACGAAGGCGCAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCGCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTGGGCGCTACGATGGGCGGGTTGATGTTACTGTACGGc                                                                                                         >  2:80493/1‑251 (MQ=255)                                                                                                                                    aTGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCg                                                                                                                             <  1:323928/230‑1 (MQ=255)                                                                                                                                    aTGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTGGGCGATACGATGGGCg                                                                                                                             >  2:323928/1‑230 (MQ=255)                                                                                                                                      ggATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTGGGCGATACGATGGGCGAGTTGATGTTACTGTACGGCAtt                                                                                                      <  1:424813/251‑1 (MQ=255)                                                                                                                                          tGCCACCAGCAATCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGGGGGCGATACGATGGGCGTGTTGATGTTACTGTACGGCCTTGCCg                                                                                                  >  2:393255/1‑251 (MQ=255)                                                                                                                                            ccaccaGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTGGGCGATACGATGGGCGAGTTGATGTTACTGTACGGCATTGCCGAt                                                                                                >  1:159359/1‑251 (MQ=255)                                                                                                                                                  gCACTCACGAAGGCGAAGATAGTGTGGTGATCGCCGCACATCAGGCATTGCTACAGCAACTCCCGAATTTATTGCTCATCCTGGTACCCCGGCAACCAGAACGCTGCCTGGATGCGATTAAACGTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCTCCAGCACGCAGGTGGTGGTGGGCGATACGATGGGCGAGTTGATATTACTGTACGTCATTGCCGATCTCGcc                                                                                          <  2:152495/251‑1 (MQ=255)                                                                                                                                                   cACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTGGGCGATACGATGGGCGAGTTGATGTTACTGTACGGCATTGCCGATCTCGCCt                                                                                         >  1:314245/1‑251 (MQ=255)                                                                                                                                                        aCGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTTGTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTGGGCGATACGATGGGCGAGTTGATGTTACTGCACGGCATTGCCGATCTCGCCTttgtt                                                                                    <  1:242195/251‑1 (MQ=255)                                                                                                                                                                    agagTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTAGTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTGGTGGTGGGCGATACGATGGGCGAGTTGATGTTACTGTACGGCATTGCCGATCTCGCCGTTGTTGGCGGTTCACTg                                                                        <  2:179341/251‑1 (MQ=255)                                                                                                                                                                        tgtgGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTGGGCGATACGATGGGCGAGTTGATGTTACTGTACGGCATTGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTg                                                                    >  2:125539/1‑251 (MQ=255)                                                                                                                                                                            gtgATCGCCGCACATCAGGCATTGTGACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCTCCAGCACGCAGGTTGTGGTGGGCGATACGATGGGCGAGTTGATGTTACTGTACGGCATTGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACg                                                                <  2:256107/251‑1 (MQ=255)                                                                                                                                                                              gATCGCCGCACATCAGGCATTGTTCCAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTGGGCGATACGATGGGCGAGTTGATGTTACTGTACGGCATTGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACgtg                                                              >  2:296621/1‑251 (MQ=255)                                                                                                                                                                                   ccgcACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTGGGCGATACGATGGGCGAGTTGATGTTACTGTACGGCATTGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTggg                                                         >  1:258740/1‑251 (MQ=255)                                                                                                                                                                                    cgcACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTGGGCGATACGATGGGCGAGTTGATGTTACTGTACGGCATTGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGc                                                        >  2:221749/1‑251 (MQ=255)                                                                                                                                                                                    cgcACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTCGTAACCCGTCATCCAGAACGCTTCCCGGCTGCGATTCATCTTGTCAGCCAGGCTGGACTAAGCTATATCACCAGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTGGGCGATACGATGGGCGAGTTGATGTTACTGTACGGCATTGCCGATCTCGCCTTTGTTGGCGGTTCACTGGGTGAACGTGGTGGGc                                                        >  1:415969/1‑251 (MQ=255)                                                                                                                                                                                               cATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTGGGCGATACGATGGGCGAGTTGATGTTACTGTACGGCATTGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGc                                                        <  1:131547/240‑1 (MQ=255)                                                                                                                                                                                               cATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTGGGCGATACGATGGGCGAGTTGATGTTACTGTACGGCATTGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGc                                                        >  2:131547/1‑240 (MQ=255)                                                                                                                                                                                                    ttACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTGGGCGATACGATGGGCGAGTTGATGTTACTGTACGGCATTGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGc                                        >  1:267692/1‑251 (MQ=255)                                                                                                                                                                                                      aCAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTGGGCGATACGATGGGCGAGTTGATGTTACTGTACGGCATTGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTg                                      >  1:259727/1‑251 (MQ=255)                                                                                                                                                                                                          cAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTGGGCGATACGATGGGCGAGTTGATGTTACTGTACGGCATTGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGc                                  >  2:252935/1‑251 (MQ=255)                                                                                                                                                                                                              tCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTGGGCGATACGATGGGCGAGTTGATGTTACTGTACGGCATTGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGcaca                               <  2:159359/250‑1 (MQ=255)                                                                                                                                                                                                               cccGAATTTATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCATCACGCAGGTTGTGGTGGGCGATACGATGGGCGAGTTGATGTTACTGTACGGCATTGCCGATCTCGCCTTTGTTGGCGGTTCTCTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCacac                              <  2:144006/250‑1 (MQ=255)                                                                                                                                                                                                                      ttATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTGGGCGATACGATGGGCGAGTTGATGTTACTGTACGGCATTGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTcc                      >  1:68380/1‑251 (MQ=255)                                                                                                                                                                                                                       tATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTGGGCGATACGATGGGCGAGTTGATGTTACTGTACGGCATTGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCg                     >  1:219828/1‑251 (MQ=255)                                                                                                                                                                                                                       tATTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAAGCTTGTCCGCCAGGCTGGACTAAGCTATATCTCACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTGGGCGATACGATGGGCGAGTTGATGTTACTGTACGGCATTGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCTCACGCTATTcc                      <  2:190323/250‑1 (MQ=255)                                                                                                                                                                                                                        aTTGCTCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTGGGCGATACGATGGGCGAGTTGATGTTACTGTACGGCATTGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCgg                    >  1:375838/1‑251 (MQ=255)                                                                                                                                                                                                                             tCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTGGGCGATACGATGGGCGAGTTGATGTTACTGTACGGCATTGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCt                                       >  2:373584/1‑227 (MQ=255)                                                                                                                                                                                                                             tCATCCTGGTACCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTGGGCGATACGATGGGCGAGTTGATGTTACTGTACGGCATTGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCt                                       <  1:373584/227‑1 (MQ=255)                                                                                                                                                                                                                                   gggTACCCCGTCATCCAGAAAGCTTCCCGGATTCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCCCTCCAGGGGAAGGCCCCTCGACCAGCACGCAGGTTGTGGTGGGCGATTCGATGGGCGAGTTGATGTTACTGGTCGGCATGTCCGATGCCGTCTTTGTTGGCGATTCTCTGGTTGAGCGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACATGCTATTCCGGGATTGATgggg         <  2:415969/250‑1 (MQ=255)                                                                                                                                                                                                                                       aCCCCGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTGGGCGATACGATGGGCGAGTTGATGTTACTGTACGGCATTGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGc     >  1:277970/1‑251 (MQ=255)                                                                                                                                                                                                                                         cccGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTGGGCGATACGATGGGCGAGTTGATGTTACTGTACGGCATTGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCAt   <  2:348174/251‑1 (MQ=255)                                                                                                                                                                                                                                          ccGTCATCCAGAACGCTTCCCGGATGCGATTAATCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTGGGCGATACGATGGGCGAGTTGATGTTACTGTACGGCATTGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATa  <  2:430504/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  TTTGTGGCGCTGGGCGCAAAAAATAATCAGGTGACCGTTACCGGTAGCCTGAAATTCGATATTTCTGTAACGCCGCAGTTGGCTGCTAAAGCCGTGACGCTGCGCCGCCAGTGGGCACCACACCGCCCGGTATGGATTGCCACCAGCACTCACGAAGGCGAAGAGAGTGTGGTGATCGCCGCACATCAGGCATTGTTACAGCAATTCCCGAATTTATTGCTCATCCTGGTACCCCGTCATCCGGAACGCTTCCCGGATGCGATTAACCTTGTCCGCCAGGCTGGACTAAGCTATATCACACGCTCTTCAGGGGAAGTCCCCTCCACCAGCACGCAGGTTGTGGTTGGCGATACGATGGGCGAGTTGATGTTACTGTATGGCATTGCCGATCTCGCCTTTGTTGGCGGTTCACTGGTTGAACGTGGTGGGCATAATCCGCTGGAAGCTGCCGCACACGCTATTCCGGTATTGATGGGGCCGCATA  >  NC_000913/3809110‑3809593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑