Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,358,8590TC95.0% 48.7 / ‑6.2 20Y110C (TAT→TGT) puuPputrescine:H(+) symporter PuuP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (7/12);  minor base A (0/1);  total (7/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.73e-01

CAGACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGG  >  NC_000913/1358613‑1359072
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                      
cAGACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCCAAAAGAGACAATc                                                                                                                                                                                                                    >  1:118100/1‑251 (MQ=255)
       tGCCAACGACTTCTCCTTTATGCAGTCACTGAACTACCAGGCAGATAAACACAACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCCGGTTGCCGCTCTTCAGATTCGCGGCGGTTAAAATGGCGAAGGAGGATACAACCCACACCCAAGCGGGCACTTCCGGGCAGAGGGAGGAGAGATAGGTTTTAGCCAAAAAGAAGTTCATCAACGGCACAAAAAGACAATCCagaagc                                                                                                                                                                                                             >  1:421352/1‑251 (MQ=255)
         ccAACGCCTTCTCCGTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAAGCCAGCAGCGa                                                                                                                                                                                                           <  2:133647/251‑1 (MQ=255)
                        ttATGCAGTCCCTGAACTACCAGGAAGATAATCACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCTCACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCGGCCGAcc                                                                                                                                                                                              <  2:380195/249‑1 (MQ=255)
                         tATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCAt                                                                                                                                                                                            <  1:111431/250‑1 (MQ=255)
                                    tGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCCCCCACACCCACGGCGGCACTTTCGGGGAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGGTGACCAGCCGACCATAAATCCGAcc                                                                                                                                                                                  >  2:84394/1‑248 (MQ=255)
                                                    tAAACACCACCATGATGGAGATTTGCACCAGTACAAAAAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAAAGGCGCCGAAGGTTACCACCCACACCCCCGGCGGCCCTTCCGGGAAGAGGGCGGGGAGATAGATTTTCCCCAACAAGACGTTAATCAACGGCAAAAAGAGACAATACAGCCGCGCTGACCAGCCCCACATAAATCCGACCAGCGGGtgaaacgaattt                                                                                                                                                                >  2:286904/1‑240 (MQ=255)
                                                                     gagaTTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATg                                                                                                                                                                                                         <  1:331610/192‑1 (MQ=255)
                                                                       gaTTTGCCCCAGTACAAACTGGGTATTGAAGTTAGCGACCAGGTTGACTCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGGTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCCAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCAGAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGctg                                                                                                                                             <  2:246357/251‑3 (MQ=255)
                                                                            gCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAAcc                                                                                                                                         <  1:352461/250‑1 (MQ=255)
                                                                                            ggTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGc                                                                                                                        <  2:118100/251‑1 (MQ=255)
                                                                                                                                      ggcggTTAAAATGGCGACGAAGGTTACCACCCACACCCACGTCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAACCTGGCGAACCAGTCTGCCGTAGCTGATATCGGTAAACAGCACTCCcg                                                                             <  2:295390/251‑1 (MQ=255)
                                                                                                                                         ggTTACAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCcgcc                                                                           >  2:381053/1‑250 (MQ=255)
                                                                                                                                                           gggtACCACCCACCCCCTCGGCGGCACTCCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAAGCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAACCGGGCGACCCAGTTTGCCGTAGCTGAAAGCGGTAAACATCACCCCCGCCAGCGCCAGCAAATAGGACg                                                        <  2:141258/248‑1 (MQ=255)
                                                                                                                                                                                  gcactgcggggaggggggCGGAGAAATAGATTTTCGCCAACAGGACGTTAACCATCGGAAAAAAGAGAAAATCCAGCAGGGATGCCCAGCCGACCAAAAATCCGACGGGCGGGTAAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCGTTGGGCCGTAGCGGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCTCTAATg                                 <  2:303282/236‑1 (MQ=255)
                                                                                                                                                                                         gggaaagggggCGGATAGATAGATTTCCGCCAACAAGACGTAAATCATCGGCAAAAAGAGACAATCCAGCAGCTATGGCCAGCCGACCATAAACCGGACGTGCGGGTAACGGGGCTTTTGCGCGTAGGTATAGGCCGAGCCGGGCCGTGGAAACTGGCGAACCAGGTTGCGGTGGCTGGTAGCGGTAAACAGCACCCCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGgaga                          <  1:432436/243‑1 (MQ=255)
                                                                                                                                                                                         cGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCgg                             >  1:124054/1‑248 (MQ=255)
                                                                                                                                                                                                agGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATAcc                   >  1:133699/1‑251 (MQ=255)
                                                                                                                                                                                                           atagatTTTCGCAAACAAGACGTTAACCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCAAAAACCCGACGGGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCAGGAAACGGGCGACCCAGTTTGCCGTAGCGGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCaa        <  1:286904/251‑1 (MQ=255)
                                                                                                                                                                                                                  ttCGCAAAAAAGACGTAAATCATCGGAAAAAAGAGACAATCCAGCAGCGATGCCCAGCCGACCATAAATCCGACGTGCGGTTTAATCGACTTTTGCGCGTGGGTATAGGCCGAACCGGCCCCCGGAAACTGGCGAACCAGTTTGCCGTAGCGGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACgg  <  1:300728/250‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                      
CAGACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGG  >  NC_000913/1358613‑1359072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: