Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,808,878	A→G	A113A (GCA→GCG)	waaA →	KDO transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,808,878	0	A	G	100.0%	189.2 / NA	55	A113A (GCA→GCG)	waaA	KDO transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/28);  total (27/28)

GGGTGAACGTTACGGTTTTTACCGCCATCCGCTAAAACCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGGCCGCAGGTTATGCCAAACTGGGTAAATTCGTCCGTCGCTTGCTGCGTCGTATTACGCTGATTGCTGCGCAAAATGAAGAAGATGGTGCACGTTTTGTGGCGCTGG  >  NC_000913/3808644‑3809122                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                     gggTGAACGTTACGGTTTTTACCGCCATCCGCTAAAACCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTg                                                                                                                                                                                                                                       >  1:85052/1‑250 (MQ=255)   gTGAACGTTACGGTTTTTACCGCCATCCGCTAAAACCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAAt                                                                                                                                                                                                                                    <  2:61138/251‑1 (MQ=255)         gTTACGGTTTTTACCGCCATCCGCTTAAACCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTc                                                                                                                                                                                                                              >  2:182821/1‑251 (MQ=255)             cGGTTTTTACCGCCATCCGCTAAAACCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGGTCAGCACGTTTATCTTCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGAcc                                                                                                                                                                                                                          <  1:414658/251‑1 (MQ=255)                   ttACCGCCATCCGCTAAAACCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGCTGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTacac                                                                                                                                                                                                                    >  1:230792/1‑248 (MQ=255)                     aCCGCCATCCGCTAAAACCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACt                                                                                                                                                                                                                   <  1:2411/250‑1 (MQ=255)                       cGCCATCCGCTAAAACCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGgt                                                                                                                                                                                                                >  1:47186/1‑251 (MQ=255)                                 tAAAACCAGGCGGCATTATGCTGCAATCAGTCTCCGTCGGTGAAACTATGGCGGCGATCACGTTGGTGCGCGCGCTGCGTCATAGTTATCCTGATTTACCGCTTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCAGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCACCCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATg                                                                                                                                                                                                      >  2:262609/1‑251 (MQ=255)                                  aaaaCCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTAt                                                                                                                                                                                                       <  1:300529/249‑1 (MQ=255)                                  aaaaCCAGGCGGCATTATGCTGCACTCCGTCTACGTCGGGGAAACTCTGGCGGCGATCCCGTTGGAGCGCGCGCTGCGTCATCGTTATCCTGTTTTACCGATTACCGTTACAACCACGACGCCAACCGGTTCGGATCGCGTACAATCGGCTTTCGGGAAGCATGTTCAGCACGTCTATCAGCCGTATGACCTGCCCGATGCGCTCAACAGTATCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATgg                                                                                                                                                                                                     >  2:242195/1‑251 (MQ=255)                                     aCCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTgg                                                                                                                                                                                                                 >  2:80440/1‑236 (MQ=255)                                     aCCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTgg                                                                                                                                                                                                                 <  1:80440/236‑1 (MQ=255)                                                 tATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGc                                                                                                                                                                                      >  1:124556/1‑251 (MQ=255)                                                  aTGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGcc                                                                                                                                                                                     >  2:223410/1‑251 (MQ=255)                                                        cACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCt                                                                                                                                                                               <  1:298650/251‑1 (MQ=255)                                                        cACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGAGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTAGGGCCTAACCt                                                                                                                                                                               <  2:60762/251‑1 (MQ=255)                                                                    gtcGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCt                                                                                                                                                                   <  2:259753/251‑1 (MQ=255)                                                                      cGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTAc                                                                                                                                                                 <  2:300270/251‑1 (MQ=255)                                                                       ggTGAAAATATGGCGGCGCTCCCGTTGGTGAGCCCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTAc                                                                                                                                                                 >  1:121099/1‑250 (MQ=255)                                                                                ctGGCGGCGATCTAGTTGGTGCGCGCGCTGCGTCATCGTTATCCGGATTTACCGATGACCGTTATAACCATGACGCCAACCGGTTTGGAGCGCGTACAATCGGCTTTCGGGAAGGATGGTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCGCAAACGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGTGGCGCTACATAAACGt                                                                                                                                                         <  1:438734/249‑1 (MQ=255)                                                                                   gcggcTATCCCGTTTGTGCGCGAGCTGCGTCATCGTTATCCGGATTTACCGATGTCCGTGACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCGGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTaatat                                                                                                                                                    <  2:347177/251‑4 (MQ=255)                                                                                    cggcGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAAtt                                                                                                                                                   >  1:173414/1‑251 (MQ=255)                                                                                         aTCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCt                                                                                                                                              >  1:32600/1‑251 (MQ=255)                                                                                              gTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATaa                                                                                                                                                                                                                                  <  1:362476/162‑1 (MQ=255)                                                                                              gTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATaa                                                                                                                                                                                                                                  >  2:362476/1‑162 (MQ=255)                                                                                                       cgcgcTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAAcg                                                                                                                                <  1:155923/251‑1 (MQ=255)                                                                                                                  aTCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGGGATCGCTAACGCGCGActctct                                                                                                                     >  1:243210/1‑251 (MQ=255)                                                                                                                  aTCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGCAAAATTCCGCTGGTGATCGCTAACGCGCGActctct                                                                                                                     <  2:47186/251‑1 (MQ=255)                                                                                                                       tagccTGAATTACCGATTACCGGTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGTCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGATAACGCGCGACTCTCTGccc                                                                                                                 <  2:59721/247‑1 (MQ=255)                                                                                                                          ccTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTc                                                                                                             <  1:266945/251‑1 (MQ=255)                                                                                                                            tGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCgg                                                                                                           >  1:27979/1‑251 (MQ=255)                                                                                                                                             ttacaacCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGGCCGCAGGTTATGCCaa                                                                                           >  1:373452/3‑250 (MQ=255)                                                                                                                                               acaacCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGGCCGCAGGTTATGCCAAACt                                                                                        >  1:279318/1‑251 (MQ=255)                                                                                                                                                caacCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACTCGCGACTCTCTGCCCGCTCGGCCGCAGGTTATGCCAAACTg                                                                                       <  2:27979/251‑1 (MQ=255)                                                                                                                                                 aacCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGGACGCAGGTTATGCCAAACTgg                                                                                      >  1:416088/1‑251 (MQ=255)                                                                                                                                                           ccAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTGGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGGCCGCAGGTTATGCCAAACTGGGTAAATTcgt                                                                            <  1:59154/251‑1 (MQ=255)                                                                                                                                                                cGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGGCCGCAGGTTATGCCAAACTGGGTAAATTcgtccgtc                                                                       >  2:371931/1‑251 (MQ=255)                                                                                                                                                                            cgTACAATCGGCTGTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGGCCGCAGGTTATGCCAAACTGGGTAAATTCGTCCGTCGCTTGCTGcgtc                                                           <  2:11578/251‑1 (MQ=255)                                                                                                                                                                                cAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCGCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGGCCGCAGGTTATGCCAAACTGGGTAAATTCGTCCGTCGCTTGCTGCGTCGTAt                                                       <  1:3465/251‑1 (MQ=255)                                                                                                                                                                                  aTCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGGCCGCAGGTTATGCCAAACTGGGTAAATTCGTCCGTCGCTTGCTGCGTCGTAtt                                                      >  1:207185/1‑250 (MQ=255)                                                                                                                                                                                         ttCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGGCCGCAGGTTATGCCAAACTGGGTAAATTCGTCCGTCGCTTGCTGCGTCGTATTACGCTGAt                                              >  2:89128/1‑251 (MQ=255)                                                                                                                                                                                            gggAAGGATGTTCAGCACGTTTATCTGCCGTATGCCCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAACCGTAAAATTCCGCTGGTTATCGCTAACGCGCGACTCTCTGCCCGCTCGGCCGCAGGTTATGCCAAACTGGGTAAATTCGTCCTTCTCTTGCTGCGTCGTATTACGCTGATtgc                                           <  2:265978/251‑1 (MQ=255)                                                                                                                                                                                              gAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGGCCGCAGGTTATGCCAAACTGGGTAAATTCGTCCGTCGCTTGCTGCGTCGTATTACGCTGATtgccg                                         <  2:85052/251‑3 (MQ=255)                                                                                                                                                                                                         aGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGGCCGCAGGTTATGCCAAACTGGGTAAATTCGTCCGTCGCTTGCTGCGTCGTATTACGCTGATTGCCGCCCAAAATgaa                              <  1:124840/251‑1 (MQ=255)                                                                                                                                                                                                              gTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGGCCGCAGGTTATGCCAAACTGGGTAAATTCGTCCGTCGCTTGCTGCGTCGTATTACGCTGATTGCCGCCCAAAATgaaga                            >  1:429876/1‑248 (MQ=255)                                                                                                                                                                                                                 tATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGGCCGCAGGTTATGCCAAACTGGGTAAATTCGTCCGTCGCTTGCTGCGTCGTATTACGCTGATTGCCGCCCAAAATGAAGAAGATg                       <  1:89128/250‑1 (MQ=255)                                                                                                                                                                                                                      gCCGTATGACCTGCCCGAGGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCGAACCTGATTGCGGCGTTACATAAACGTAAAATTCCGCTGGTGAGCGCTAACGCGCGACGCTCTGCCCGCTCGGCCGCAGGTGATGCCAAACTGGGTAAATTCGTCCGTCGCTTGCTGCGTCGTATTACGCTGATTGCCGCCCAAAATGAAGAAGATGGTg                    <  2:209947/248‑1 (MQ=255)                                                                                                                                                                                                                       ccGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGGCCGCAGGTTATGCCAAACTGGGTAAATTCGTCCGTCGCTTGCTGCGTCGTATTACGCTGATTGCCGCCCAAAAt                                 <  1:251609/234‑1 (MQ=255)                                                                                                                                                                                                                       ccGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGGCCGCAGGTTATGCCAAACTGGGTAAATTCGTCCGTCGCTTGCTGCGTCGTATTACGCTGATTGCCGCCCAAAAt                                 >  2:251609/1‑234 (MQ=255)                                                                                                                                                                                                                       ccGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGGCCGCAGGTTATGCCAAACTGGGTAAATTCGTCCGTCGCTTGCTGCGTCGTATTACGCTGATTGCCGCCCAAAATGAAGAAGATGGTGCACg                >  2:408724/1‑251 (MQ=255)                                                                                                                                                                                                                          tATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGGCCGCAGGTTATGCCAAACTGGGTAAATTCGTCCGTCGCTTGCTGCGTCGTATTACGCTGATTGCCGCCCAAAATGAAGAAGATGGTGCACGttt             >  2:311668/1‑251 (MQ=255)                                                                                                                                                                                                                           aTGACCTGCCCGATGCGCTCAACCGTCTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGGCCGCAGGGTATGCCAAACTGGGAAAATTCGTCCGTCGCTTGCTGCGTCGTATTACGCTGATTGCCGCCCAAAATGAAGAAGATGGTGCACGtttt            >  2:177895/1‑251 (MQ=255)                                                                                                                                                                                                                                 tGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTc                                                                                                                                                  >  2:351485/1‑111 (MQ=255)                                                                                                                                                                                                                                     cGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGGCCGCAGGTTATGCCAAACTGGGTAAATTCGTCCGTCGCTTGCTGCGTCGTATTACGCTGATTGCCGCCCAAAATGAAGAAGATGGTGCACGTTTTGTGGCGCTgg  <  1:371931/251‑1 (MQ=255)                                                                                                                                                                                                                                     cGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGGCCGCAGGTTATGCCAAACTGGGTAAATTCGTCCGTCGCTTGCTGCGTCGTATTACGCTGATTGCCGCCCAAAATGAAGAAGATGGTGCACGTTTTGTGGCGCTgg  <  1:341556/251‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                     GGGTGAACGTTACGGTTTTTACCGCCATCCGCTAAAACCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGGCCGCAGGTTATGCCAAACTGGGTAAATTCGTCCGTCGCTTGCTGCGTCGTATTACGCTGATTGCTGCGCAAAATGAAGAAGATGGTGCACGTTTTGTGGCGCTGG  >  NC_000913/3808644‑3809122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑