Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,808,785	A→T	V82V (GTA→GTT)	waaA →	KDO transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,808,785	0	A	T	98.2%	185.6 / NA	54	V82V (GTA→GTT)	waaA	KDO transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (31/22);  minor base G (0/1);  total (31/23)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GCTCGAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATACGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCATCCGCTAAAACCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGG  >  NC_000913/3808542‑3809017                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                         gCTCGAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATACGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCATCCGCTAAAACCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAAcc                                                                                                                                                                                                                                    >  1:259753/1‑250 (MQ=255)     gAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATACGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCATCCGCTAAAACCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATgcc                                                                                                                                                                                                                               >  2:341556/1‑249 (MQ=255)        ttGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATACGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCATCCGCTAAAACCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCAtaccgcc                                                                                                                                                                                                                            >  2:2411/1‑245 (MQ=255)                cacCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATACGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCATCCGCTAAAACCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGACACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGtt                                                                                                                                                                                                                    >  1:209947/1‑251 (MQ=255)                 cccGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATACGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCATCCGCTAAAACCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTc                                                                                                                                                                                                                   <  1:26481/250‑1 (MQ=255)                   cGCCCTTCTCTACATTATTCAGCCGCTGATCTGGATAAGGCTCTGGGTGCGCGGACGTAAGGCTCAGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCCTCCGCTAAAACCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCgg                                                                                                                                                                                                                 >  2:414658/1‑251 (MQ=255)                            ctACCTTATTCAGCCGCTGATCTGGATACGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCATCCGCTAAAACCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTAc                                                                                                                                                                                                        >  1:125359/1‑251 (MQ=255)                             tACCTTCTTAAGCCGCTGATCTGGATCCGGCTCTGGGAGCGCGGACCTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCATCCGCTAAAACCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTaa                                                                                                                                                                                                        >  2:59154/1‑249 (MQ=255)                              aCCTTATTCAGACGCTGATCTGGATACGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTCCGGTTTTTACCGCCATCCGCTAAAACCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTAcc                                                                                                                                                                                                       >  2:155923/1‑249 (MQ=255)                                          cGCTGATCTGGATACGGCTCGGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCATCCGCTAAAACCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCggg                                                                                                                                                                                          <  2:107632/251‑1 (MQ=255)                                             tGATCTGGATACGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCATCCGCTAAAACCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGa                                                                                                                                                                                         >  1:294149/1‑249 (MQ=255)                                                     aTACGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCATCCGCTAAAACCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACACTCGGCTTTCGGGAAGGATGTTc                                                                                                                                                                                >  1:240387/1‑250 (MQ=255)                                                     aTACGGCTCGGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCATCCGCTAAAACCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCa                                                                                                                                                                               <  2:125359/251‑1 (MQ=255)                                                       aCGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCATCCGCTAAAACCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAg                                                                                                                                                                              <  2:294837/250‑1 (MQ=255)                                                                          cGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCATCCGCTAAAACCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAAcc                                                                                                                                                                                                                                    >  2:340789/1‑177 (MQ=255)                                                                          cGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCATCCGCTAAAACCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAAcc                                                                                                                                                                                                                                    <  1:340789/177‑1 (MQ=255)                                                                             aaggacccGGACTATCGAAACCGCTGGGGCGAACGTTACGGTTTTTACCGCCCTCCGCTAAAACCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTAACGTTACAACCATGACGCCAACCGGTTCGGAGCGCGAACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGaccc                                                                                                                                                       >  2:284517/7‑248 (MQ=255)                                                                             aaGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCATCCGCTAAAACCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGacct                                                                                                                                                       >  2:124840/1‑248 (MQ=255)                                                                              aGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCATCCGCTAAAACCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTg                                                                                                                                                      >  2:300529/1‑251 (MQ=255)                                                                                    cGGCCTATCGAAAACGCTGGGGGGAACGTTACGGTTTTGACCGCCACCCGCTAAAACCAGGCGGCATTATGCTGCTCTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGAGTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCTTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTAGCTGCCGTATGACCTGCCCGAt                                                                                                                                                <  2:438018/251‑1 (MQ=255)                                                                                                       gggTGAACGTTACGGTTTTTACCGCCATCCGCTAAAACCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTg                                                                                                                              >  1:85052/1‑250 (MQ=255)                                                                                                         gTGAACGTTACGGTTTTTACCGCCATCCGCTAAAACCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAAt                                                                                                                           <  2:61138/251‑1 (MQ=255)                                                                                                               gTTACGGTTTTTACCGCCATCCGCTTAAACCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTc                                                                                                                     >  2:182821/1‑251 (MQ=255)                                                                                                                   cGGTTTTTACCGCCATCCGCTAAAACCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGGTCAGCACGTTTATCTTCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGAcc                                                                                                                 <  1:414658/251‑1 (MQ=255)                                                                                                                         ttACCGCCATCCGCTAAAACCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGCTGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTacac                                                                                                           >  1:230792/1‑248 (MQ=255)                                                                                                                          tACCGCCAGCCGCTAAAACCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATACTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGACGGATGTTCAGCACGTTTATCTGCCGTa                                                                                                                                                             >  1:269351/1‑200 (MQ=255)                                                                                                                           aCCGCCATCCGCTAAAACCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACt                                                                                                          <  1:2411/250‑1 (MQ=255)                                                                                                                             cGCCATCCGCTAAAACCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGgt                                                                                                       >  1:47186/1‑251 (MQ=255)                                                                                                                                       tAAAACCAGGCGGCATTATGCTGCAATCAGTCTCCGTCGGTGAAACTATGGCGGCGATCACGTTGGTGCGCGCGCTGCGTCATAGTTATCCTGATTTACCGCTTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCAGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCACCCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATg                                                                                             >  2:262609/1‑251 (MQ=255)                                                                                                                                        aaaaCCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTAt                                                                                              <  1:300529/249‑1 (MQ=255)                                                                                                                                        aaaaCCAGGCGGCATTATGCTGCACTCCGTCTACGTCGGGGAAACTCTGGCGGCGATCCCGTTGGAGCGCGCGCTGCGTCATCGTTATCCTGTTTTACCGATTACCGTTACAACCACGACGCCAACCGGTTCGGATCGCGTACAATCGGCTTTCGGGAAGCATGTTCAGCACGTCTATCAGCCGTATGACCTGCCCGATGCGCTCAACAGTATCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATgg                                                                                            >  2:242195/1‑251 (MQ=255)                                                                                                                                           aCCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTgg                                                                                                        >  2:80440/1‑236 (MQ=255)                                                                                                                                           aCCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTgg                                                                                                        <  1:80440/236‑1 (MQ=255)                                                                                                                                                 cggcATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATg                                                                                                                                                                                   <  2:15040/155‑1 (MQ=255)                                                                                                                                                 cggcATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATg                                                                                                                                                                                   >  1:15040/1‑155 (MQ=255)                                                                                                                                                       tATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGc                                                                             >  1:124556/1‑251 (MQ=255)                                                                                                                                                        aTGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGcc                                                                            >  2:223410/1‑251 (MQ=255)                                                                                                                                                              cACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCt                                                                      <  1:298650/251‑1 (MQ=255)                                                                                                                                                              cACTCCGTCTCCGTCGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGAGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTAGGGCCTAACCt                                                                      <  2:60762/251‑1 (MQ=255)                                                                                                                                                                          gtcGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCt                                                          <  2:259753/251‑1 (MQ=255)                                                                                                                                                                            cGGTGAAACTCTGGCGGCGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTAc                                                        <  2:300270/251‑1 (MQ=255)                                                                                                                                                                             ggTGAAAATATGGCGGCGCTCCCGTTGGTGAGCCCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTAc                                                        >  1:121099/1‑250 (MQ=255)                                                                                                                                                                                      ctGGCGGCGATCTAGTTGGTGCGCGCGCTGCGTCATCGTTATCCGGATTTACCGATGACCGTTATAACCATGACGCCAACCGGTTTGGAGCGCGTACAATCGGCTTTCGGGAAGGATGGTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCGCAAACGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGTGGCGCTACATAAACGt                                                <  1:438734/249‑1 (MQ=255)                                                                                                                                                                                         gcggcTATCCCGTTTGTGCGCGAGCTGCGTCATCGTTATCCGGATTTACCGATGTCCGTGACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCGGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTaatat                                           <  2:347177/251‑4 (MQ=255)                                                                                                                                                                                          cggcGATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAAtt                                          >  1:173414/1‑251 (MQ=255)                                                                                                                                                                                               aTCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCt                                     >  1:32600/1‑251 (MQ=255)                                                                                                                                                                                                    gTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATaa                                                                                                                         >  2:362476/1‑162 (MQ=255)                                                                                                                                                                                                    gTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATaa                                                                                                                         <  1:362476/162‑1 (MQ=255)                                                                                                                                                                                                             cgcgcTGCGTCATCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAAcg                       <  1:155923/251‑1 (MQ=255)                                                                                                                                                                                                                        aTCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGGGATCGCTAACGCGCGActctct            >  1:243210/1‑251 (MQ=255)                                                                                                                                                                                                                        aTCGTTATCCTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGCAAAATTCCGCTGGTGATCGCTAACGCGCGActctct            <  2:47186/251‑1 (MQ=255)                                                                                                                                                                                                                             tagccTGAATTACCGATTACCGGTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGTCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGATAACGCGCGACTCTCTGccc        <  2:59721/247‑1 (MQ=255)                                                                                                                                                                                                                                ccTGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTc    <  1:266945/251‑1 (MQ=255)                                                                                                                                                                                                                                  tGATTTACCGATTACCGTTACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGACCTGCCCGATGCGCTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCgg  >  1:27979/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                         GCTCGAATTGCTTTACACCGCCCTTCTCTACCTTATTCAGCCGCTGATCTGGATACGGCTCTGGGTGCGCGGACGTAAGGCTCCGGCCTATCGAAAACGCTGGGGTGAACGTTACGGTTTTTACCGCCATCCGCTAAAACCAGGCGGCATTATGCTGCACTCCGTCTCCGTCGGTGAAACTCTGGCGGCAATCCCGTTGGTGCGCGCGCTGCGTCATCGTTATCCTGATTTACCGATTACCGTAACAACCATGACGCCAACCGGTTCGGAGCGCGTACAATCGGCTTTCGGGAAGGATGTTCAGCACGTTTATCTGCCGTATGATCTGCCCGATGCACTCAACCGTTTCCTGAATAAAGTCGACCCTAAACTGGTGTTGATTATGGAAACCGAACTATGGCCTAACCTGATTGCGGCGCTACATAAACGTAAAATTCCGCTGGTGATCGCTAACGCGCGACTCTCTGCCCGCTCGG  >  NC_000913/3808542‑3809017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑