Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,796,821	A→T	P281P (CCA→CCT)	waaC →	ADP‑heptose:LPS heptosyltransferase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,796,821	0	A	T	100.0%	20.3 / NA	8	P281P (CCA→CCT)	waaC	ADP‑heptose:LPS heptosyltransferase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (2/6);  total (2/6)

GGCTGATTCAGGAATACGGATTAAACTTCCGTGGGGCGCGCCGCATGAGGAAGAACGGGCGAAACGACTGGCGGAAGGATTTGCTTATGTTGAAGTATTGCCGAAGATGAGTCTGGAAGGCGTTGCCCGCGTGCTGGCCGGGGCTAAATTTGTAGTGTCGGTGGATACGGGGTTAAGCCATTTAACGGCGGCACTGGATAGACCCAATATCACGGTTTATGGACCAACCGAT‑CCGGGATTAATTGGTGGGTATGGGAAGAATCAGATGGTATGTAGGGCTCCAAGA  >  NC_000913/3796596‑3796881                                                                                                                                                                                                                                  |                                                              ggCTGAGTTAGGAATACGGATTAAACTTCCGTGGGGCGCGCCGCATGAGGAAGAACGGGCGAAACGGCTGGCGGAAGGATTTGCTTATGTTGAAGTATGGCCGAAGATGAGTCTGGAAGGCGTTGCCCGCGTGCTGGCCGGGGCTAAATTTGTAGTGTCAGTGGATACGGGGTTAAGCCATTTAACAGCGGCACTGGATAGACCCAATATCACGGTTTATGGACCTACCGAT‑CCGGGATTAATtggtgg                                       <  1:288267/249‑1 (MQ=255)      aTTCAGGAATACGGATTAAACTTCCGTGGGGCGCGCCGCATGAGGAAGAACGGGCGAAACGACTGGCGGAAGGATTTGCTTATGTTGAAGTATTGCCGAAGATGAGTCTGGAAGGCGTTGCCCGCGTGCTGGCCGGGGCTAAATTTGTAGTGTCAGTGGATACGGGGTTAAGCCATTTAACAGCGGCACTGGATAGACCCAATATCACGGTTTATGGACCTACCGAT‑CCGGGATTAATTGGTGGGTATggg                                >  1:109764/1‑251 (MQ=255)        tCAGGAATACGGATTAAACTTCCGTGGGGCGCGCCGCATGAGGAAGAACGGGCGAAACGACTGGCGGAAGGATTTGCTTATGTTGAAGTATTGCCGAAGATGAGTCTGGAAGGCGTTGCCCGCGTGCTGGCCGGGGCTAAATTTGTAGTGTCAGTGGATACGGGGTTAAGCCATTTAACAGCGGCACTGGATAGACCCAATATCACGGTTTATGGACCTACCGAT‑CCGGGATTAATTGGTGGGTATGGga                               <  2:109764/250‑1 (MQ=255)                 cGGATTAAACTTCCGTGGGGCGCGCCGCATGAGGAAGAACGGGCGAAACGACTGGCGGAAGGATTTGCTTATGTTGAAGTATTGCCGAAGATGAGTCTGGAAGGCGTTGCCCGCGTGCTGGCCGGGGCTAAATTTGTAGTGTCAGTGGATACGGGGTTAAGCCATTTAACAGCGGCACTGGATAGACCCAATATCACGGTTTATGGACCTACCGAT‑CCGGGATTAATTGGTGGGTATGGGAAGAATCAGAt                     <  1:283719/251‑1 (MQ=255)                  ggATTAAACTTCCGTGGGGCGCGCCGCATGAGGAAGAACGGGCGAAACGACTGGCGGAAGGAGTTGCTTATGTTGAAGTAGTGCCGAAGATGAGTCTGGAAGGCGTTGCCCGCGTGCTGGCCGGGGCTAAAGTTGTAGTGTCAGTGGATACGGGGTTAAGCCATTTAACAGCGGCACTGGATAGACCCAATATCACGGTGTATGGACCTACCGAT‑CCGGGATTAATTGGGGGTTATGGGAAGAATCAGATg                    <  2:174758/251‑1 (MQ=255)                     ttAAACTTCCGTGGGGCGCGCCGCATGAGGAAGAACGGGCGAAACGACTGGCGGAAGGATTTGCTTATGTTGAAGTATTGCCGAAGATGAGTCTGGAAGGCGTTGCCCGCGTGCTGGCCGGGGCTAAATTTGTAGTGTCAGTGGATACGGGGTTAAGCCATTTAACAGCGGCACTGGATAGACCCAATATCACGGTTTATGGACCTACCGAT‑CCGGGATTAATTGGTGGGTATGGGAAGAATCAGATGGtt                 >  1:280539/1‑250 (MQ=255)                        aaCTTCCGTGGGGCGCGCCGCATGAGGAAGAACTGGCGATACGACTGGCGGAAGGATTTGCGTATGTTGAAGTATTGGCTAAGATGAGTCTGGAAGTCGTTGCTCGCGTGCTGTCCGGGGCTAAATTTGGAGTTTCAGTGGATACGGGGTTAGGCCCTTTAACGGCGGCAATGGATGGTGTCAATATCTCGGTTTATTGTACTAG‑GATTCCGGGATTAATTGATGGGTATGGGAAGAATCAGATGGTTTGt              <  1:175282/251‑1 (MQ=255)                                    gcgcgaCGCATGAGGAAGAACGGGCGAAACGACTGGCGGAAGGATTTGCTTATGTTGAAGTATTGCCGAAGATGAGTCTGGAAGGCGTTGCCCGCGTGCTGGCCGGGGCTAAATTTGTAGTGTCAGTGGATACGGGGTTAAGCCATTTAACAGCGGCACTGGATAGACCCAATATCACGGTTTATGGACCTACCGAT‑CCGGGATTAATTGGTGGGTATGGGAAGAATCAGATGGTTTGTAGGGCTccgggg  <  2:149413/251‑5 (MQ=255)                                                                                                                                                                                                                                  |                                                              GGCTGATTCAGGAATACGGATTAAACTTCCGTGGGGCGCGCCGCATGAGGAAGAACGGGCGAAACGACTGGCGGAAGGATTTGCTTATGTTGAAGTATTGCCGAAGATGAGTCTGGAAGGCGTTGCCCGCGTGCTGGCCGGGGCTAAATTTGTAGTGTCGGTGGATACGGGGTTAAGCCATTTAACGGCGGCACTGGATAGACCCAATATCACGGTTTATGGACCAACCGAT‑CCGGGATTAATTGGTGGGTATGGGAAGAATCAGATGGTATGTAGGGCTCCAAGA  >  NC_000913/3796596‑3796881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑