Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,795,909	A→G	L327L (TTA→TTG)	waaF →	ADP‑heptose‑‑LPS heptosyltransferase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,795,909	0	A	G	100.0%	150.3 / NA	47	L327L (TTA→TTG)	waaF	ADP‑heptose‑‑LPS heptosyltransferase 2
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/22);  total (25/22)

CCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGGCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTTTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑AAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCT  >  NC_000913/3795663‑3796146                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               ccTGGCGGGGGAAACACAGCTTGATCAAACGGTTTTCCTGATTGCAGCCTGTAAAGCCATTGTCACTAAAGATTCTGGCCTAATGCCCGTTGCGGCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGACCGGACTTCCCAACGCCGCTATCCCATAAAGCGCGCGTGATCCGTTCAATTCCCGGCTATCACAAAATGCGTAAAGGGGGCGCTGCGGAGGGTTATCACCAGGGCTTGATCg                                                                                                                                                                                                                                            >  2:209574/1‑251 (MQ=255)              acacAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATGCACGTTGCGGCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTcccc                                                                                                                                                                                                                                >  1:37955/1‑250 (MQ=255)                acaGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATGCACGTTGCGGCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAgc                                                                                                                                                                                                                             >  1:398355/1‑251 (MQ=255)                 catcTTGATCAAACGGTTATCCTGATTGCAGCCTGTAACGACATTGTCCCTAACGATTCTGGCCTAATGCACGTTGCGGCGGCACACAATCGTCCGCTGGTTTCCCTGTATGGTCCGCATAGCCCGGACTTAACACCGCCGATATACCATAAAACGCGCGAGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGTCGCTGCGGAGGGTTCTCACCAGCGAGTGGTCGACATTACTCCCCAgcg                                                                                                                                                                                                                            >  2:416469/4‑251 (MQ=255)                       aaTCAGGCGGTTATCCTGAGTGCGGCCTGTAAAGCCATGGTCACTAACGACTCTGGCCTAATGCACGTTGGGGCGGCGCCCAAGCGTCCGCTGTTTGCCCTGTATGGGCCGAGTAGCCGGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGAGCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTAc                                                                                                                                                                                                                       <  2:170234/249‑1 (MQ=255)                         ccAAGCGGTTATCCTGATTGCAGCTTGTAAAGCCATTGTCACTAACGTTTCTGGCCTAATGCACGTTGCGGCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTgg                                                                                                                                                                                                                    <  1:206690/250‑1 (MQ=255)                           aaGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATGCACGTTGCGGCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGga                                                                                                                                                                                                                   >  1:308962/1‑250 (MQ=255)                                  tATCCTGCTTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGTCCTAATGCACGTTGCGGCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGACCGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACACAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGAAGAACTc                                                                                                                                                                                                            >  2:283845/1‑250 (MQ=255)                                       tGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATGCACGTTGCGGCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAAcgcg                                                                                                                                                                                                      >  1:78550/1‑251 (MQ=255)                                                    taaagcatctGTCACTAGCGATTGTGGCTAAATGCACGTTGCGGTGGGGCTCACTCGCCCGCTGGTCGCCCTGTTGGGTCCGTTTCGCCCTGTCTCCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑aa                                                                                                                                                                                         <  2:87539/241‑1 (MQ=255)                                                    tAAAGCCATTGTCACTAACGATTCTGACCTAATGCACGTTGCGGCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑aa                                                                                                                                                                                         >  2:207815/1‑250 (MQ=255)                                                     aaaGCCTTTGTCACTAACTGTTCGGTCCTAATGCCCGTTGCGGCGGCCCTCAACGGTCCGCGGGTGGCCTGGTATGTTCCGAGTAGCCGGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑Aag                                                                                                                                                                                        <  1:429410/250‑1 (MQ=255)                                                             tGTCACTAACGATTCTGGCCTAATGCACGTTGCGGCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTAACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTAATGGAAGAACTCAACGCGCTATTGT‑ACTACGAGGAAGCCt                                                                                                                                                                               >  1:68302/1‑251 (MQ=255)                                                               tCACTAACGATTCTGGCCTAATGCACGTTGCGGCGGCGCTCAATCGTCCGATGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACAACGCAGCTCTCCCATAAAGCGCGCGCGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTACCGCCGCGGAGGGTTATCACCAGACCTTGATCGACATCACTCCCCAGCGCGTAATGGAAGAACTCAACGCGCTATTGTTAC‑ACGAGAAAACCTg                                                                                                                                                                              >  1:193809/1‑250 (MQ=255)                                                                cACTAACGATTCTGGCCTAATGCACGTTGCGGCTGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGAGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGGGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGACGAACTCAACGCGCTATTGTTAC‑AAGAGGACGCCTGAc                                                                                                                                                                            >  2:102405/1‑251 (MQ=255)                                                                      ggatccgggcagaaggcgcgtgggggcggcgCTCAATCGTCCGTGGGTGGCACGTTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑AAGAGGAAGCCGGACGGATGc                                                                                                                                                                      <  2:373653/227‑1 (MQ=255)                                                                           cTGGCCTAATGCACGTTGCGGCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑AAGAGGAAGCCTGACGGATGCGGGtt                                                                                                                                                                 >  1:345391/1‑251 (MQ=255)                                                                             ggcctaatgcccgTTGCGGCGGCGCTCAATGGTCCGCTGGTGGCCCTGTATGTTCCGAGTAGCCCGGATTTCGCCCCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑AAGAGGAAGCCTGACGGATGCGGGtt                                                                                                                                                                 <  1:42761/237‑1 (MQ=255)                                                                              gCCTAATGCACGTTGCGGCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑AAGAGGAAGCCTGACGGATGCGGGTTTTg                                                                                                                                                              >  2:86827/1‑251 (MQ=255)                                                                                        agaggggggggcgtccAATCGTCCGCTGGTGGCCCTGTATGTTCCGACTGGCCCGGGTTTCAAACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCATCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTTGAATAACTCATCGCGCTATTGTTAC‑AAGAGGATGCTTGACGGATGCGGGTTTTGATCGTTaaa                                                                                                                                                     <  2:46438/235‑1 (MQ=255)                                                                                                       cAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑AAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCg                                                                                                                                     >  1:75483/1‑251 (MQ=255)                                                                                                       cAATCGTCCGCTGGTTCCCCGGTATGGTCCGAGTAGCCCGTACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑AAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATggg                                                                                                                                       <  1:68298/249‑1 (MQ=255)                                                                                                                gCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑AAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTcc                                                                                                                            >  1:287234/1‑251 (MQ=255)                                                                                                                   ggTTGCCCTGTATGGTCCGAGTAGCCCGGCCTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑AAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCAt                                                                                                                          <  1:311623/250‑1 (MQ=255)                                                                                                                            gTATGGTCCGAGTAGCCCGGCCTTCCCACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑AAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGccc                                                                                                                 <  2:75483/250‑1 (MQ=255)                                                                                                                                      aGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑AAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTg                                                                                                       >  1:270425/1‑250 (MQ=255)                                                                                                                                                  ttCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑AAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGc                                                                                          >  1:377580/1‑251 (MQ=255)                                                                                                                                                      atccgccgCTGTCCCATAAAGCTCGCGTGATCCGTCTGAGTACCGGCTATCACAAAGTGCGTAAAGGTGACGCGGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGGACTGGAAGAACTCAACGCGCTATTGTTAC‑AAGATGAAGCCTGGCGGATGCGGGTTTTGATCGTTAAAACATCGTCTATCGACGATGTTCTCCATTCGTTGCCCGCTCTCACTGATGCCCAGCGGGCattc                                                                                      <  2:220690/249‑4 (MQ=255)                                                                                                                                                          gccgcTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑AAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATccc                                                                                    >  2:217794/1‑249 (MQ=255)                                                                                                                                                           ccgcTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCGTGATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑AAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAgg                                                                                 <  2:259812/251‑1 (MQ=255)                                                                                                                                                                     aTAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑AAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGttt                                                                       >  1:395261/1‑251 (MQ=255)                                                                                                                                                                         aGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑AAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACt                                                                   <  1:335142/251‑1 (MQ=255)                                                                                                                                                                               cgTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑AAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCTATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAATTTTGACTGggtgg                                                             <  1:336361/251‑1 (MQ=255)                                                                                                                                                                                     ccGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑AAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCAAACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGgaa                                                        <  1:230276/250‑1 (MQ=255)                                                                                                                                                                                       gtctGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTGCTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑AAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGgaagaa                                                     <  1:437705/248‑1 (MQ=255)                                                                                                                                                                                       gactgaatactggCTATCACAACGTGGGTAAAGGTGACCCTGCGGAGAGTTACCACCAGAGCTTGATCGAAAATACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑AAGTGGAAGCATGACGGATGCGGATTTTGCTCGTTAACACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAGTCCCGGGGATTAAGTTTGACTGGGTGGTGgaaga                                                      >  2:173339/12‑250 (MQ=255)                                                                                                                                                                                               aCCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑AAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCg                                              <  1:86827/250‑1 (MQ=255)                                                                                                                                                                                                           aaaGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑AAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGAttccttc                                 <  1:67907/251‑1 (MQ=255)                                                                                                                                                                                                             aGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑AAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATtccttcct                               <  1:203205/251‑1 (MQ=255)                                                                                                                                                                                                               tGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑AAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTgg                             >  2:90498/1‑251 (MQ=255)                                                                                                                                                                                                                 cgtaacggtgcagctccGGAGGGTTATCACAAGAGCTTGCTCGACATTACTCCCCAGCGCGTACTGGAAGAACTAAACGCGCTATTGTTAA‑AAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACCTCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTACGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGc                            >  2:187813/17‑250 (MQ=255)                                                                                                                                                                                                                          gACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACACGCTATTGTTAC‑AAGAGGAAGCCTGACGGACGCGGGTTTTGATCGATAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCCCGCTGCCGt                  >  1:434070/1‑251 (MQ=255)                                                                                                                                                                                                                                  ggAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑AAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAg          <  2:64746/251‑1 (MQ=255)                                                                                                                                                                                                                                     gggTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑AAGAGGAAGCCTGACGGATGCGGGATTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGCGGAGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGtt        >  2:336911/1‑250 (MQ=255)                                                                                                                                                                                                                                         tATCACCAGAGCTTGATCACCATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAA‑AAGAGGAAGCATGACGGATGCGGGATTTGATCGTTAAAACATCGTCGCTGGGCGATGTTCTCCATACGTTGCACGAACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCCAAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTcc   >  2:288267/1‑251 (MQ=255)                                                                                                                                                                                                                                          aTCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑AAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCGCGTTGCCGTTGAGCGAGTTATTcc   <  2:316287/250‑1 (MQ=255)                                                                                                                                                                                                                                          aTCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑AAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCt  <  2:308962/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               CCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGGCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTTTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑AAGAGGAAGCCTGACGGATGCGGGTTTTGATCGTTAAAACATCGTCGATGGGCGATGTTCTCCATACGTTGCCCGCACTCACTGATGCCCAGCAGGCAATCCCAGGGATTAAGTTTGACTGGGTGGTGGAAGAAGGGTTCGCACAGATTCCTTCCTGGCACGCTGCCGTTGAGCGAGTTATTCCT  >  NC_000913/3795663‑3796146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑