Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,795,750	T→C	H274H (CAT→CAC)	waaF →	ADP‑heptose‑‑LPS heptosyltransferase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,795,750	0	T	C	100.0%	162.3 / NA	57	H274H (CAT→CAC)	waaF	ADP‑heptose‑‑LPS heptosyltransferase 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/21);  total (36/21)

GTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCGGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATG‑CATGTTGCGGCGGCGCTCAAT‑CGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTTTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑AAGAGGAAGCCTGACGGATGCGGGTTTTG  >  NC_000913/3795503‑3795990                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                   gTCCGGCAAAACGCTGGCCACACTCCCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCCGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATG‑CACGtt                                                                                                                                                                                                                                                 >  2:216756/1‑251 (MQ=255) gTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGGTATCAGGGGGTTCTGGTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAAGACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATG‑CACGtt                                                                                                                                                                                                                                                 <  2:16873/251‑1 (MQ=255)                 gCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATG‑CACGTTGCGGCGGCGCTCAAT‑c                                                                                                                                                                                                                                <  1:109710/251‑1 (MQ=255)                         aCCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATG‑CACGTTGCGGCGGCGCTCAAT‑CGTCCGCt                                                                                                                                                                                                                         <  2:81401/250‑1 (MQ=255)                             cgaTGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCGTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATG‑CACGTTGCGGCGGCTCTCAAT‑CGTCCGCTGGTTg                                                                                                                                                                                                                    <  2:258813/249‑1 (MQ=255)                                      gCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATG‑CACGTTGCGGCGGCGCTCAAT‑CGTCCGCTGGTTGCCCTGTATg                                                                                                                                                                                                           <  1:256423/251‑1 (MQ=255)                                            aaaGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGAAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGAACAAGCGGTTATCCTGATTGCAGACTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATG‑CACGTTGCGGCGGCGCTCACT‑CGTCCTCTGGTTGCCCTTTATgttc                                                                                                                                                                                                        >  1:366772/1‑245 (MQ=255)                                                  gcTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATG‑CACGTTGCGGCGGCGCTCAAT‑CGTCCGCTGGTTGCACTGTATGGTCCGAGGAGcc                                                                                                                                                                                               >  2:331404/1‑251 (MQ=255)                                                               ggTTATCAGGTGTTTCTGTTTGGCTCTGCGAAAGATCAGGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCGGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATG‑CACGTTGCGGCGGCGCTCAAT‑CGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACAcc                                                                                                                                                                                  <  1:157634/251‑1 (MQ=255)                                                               ggTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATG‑CACGTTGCGGCGGCGCTCAAT‑CGTCCg                                                                                                                                                                                                                           <  2:22653/210‑1 (MQ=255)                                                               ggTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATG‑CACGTTGCGGCGGCGCTCAAT‑CGTCCg                                                                                                                                                                                                                           >  1:22653/1‑210 (MQ=255)                                                                   aTCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAGCCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATG‑CACGTTGCGGCGGCGCTCAAT‑CGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACAccgcc                                                                                                                                                                               <  2:299368/250‑1 (MQ=255)                                                                   aTCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATG‑CACGTTGCGGCGGCGCTCAAT‑CGTCCGCTGGTTGCCCTGTATGGGCCGAGTAGCCCGGACTTCACAccgccg                                                                                                                                                                              >  1:243876/1‑251 (MQ=255)                                                                             tCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGACGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGCTCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATG‑CACGTTGCGGCGGAGCTCCAT‑CGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCAt                                                                                                                                                                     >  1:23009/1‑250 (MQ=255)                                                                               tGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATG‑CACGTTGCGGCGGCGCTCAAT‑CGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCCTaa                                                                                                                                                                   >  2:117701/1‑250 (MQ=255)                                                                                        cagcggaggATCATGAAACGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCTGAACCTGGCGGGGGAAACACAGCTTGATCAAGAGGTTATCCTGGTTGCAGCCTGTAAAGCCATTCTCACTAACGATTCTGGCCTAATG‑CCCGTTGCGGCGGCGCTCCAT‑CCTTCGCTGGTTGCCCTTGATGGTCCGAGTGGCCCGGACTTCACCCCGCCGCTATcccctagaggccgcgtg                                                                                                                                                         >  2:180347/9‑237 (MQ=255)                                                                                          gCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATG‑CACGTTGCGGCGGCGCTCAAT‑CGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGGGAt                                                                                                                                                       >  1:277788/1‑251 (MQ=255)                                                                                           gaaagaacaaTGAAGCGGGAAATGAGATTCTGGCCGCTTTAAATACCGGGCAGCAGGCATGGTTTGGGAACCGGGCGGGGGAACCACAGCTTGACCAAGCGTTTACCCGGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCGGGCCTAAGG‑CCCGTTGCGGCGCCGCTCAAT‑CTTCCGCTGGTGGCCCTGTATGGTCCGAGTAGCCCGGATTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATc                                                                                                                                                      <  2:369772/242‑1 (MQ=255)                                                                                               aGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGCTTATCCTGATTGCAGCCTGGAAAGCCATTGTCACTAACGATTCTGGCCTAATG‑CACGTTGCGGCGGCGTTCAAT‑CGTCCGCTGGTTGCCCTGTATGGTCCGTGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGCACGCGGGAGCCGtc                                                                                                                                                  >  2:122714/1‑250 (MQ=255)                                                                                                gATCATGAAGCGGACAATGAGATTCTTGCAGATTTGAATACCGAGCAGCAGGCATGGTATCGGAACCTGGAGGGGGAAACACAGCTTGATAAAGCGGTTATCCTGATTGCAGCCTGTAAAGAAATTGTGAATAACGATTCTGGCCTAATG‑CACCTTGCGGCGCCGCTCCAT‑CGTCCGCTGGCTGCCCTGCATGGTCCGCGTAGCCCGGACTTCACACCGCAGCTCTCCCATAAACcgcgcgcgctcagccc                                                                                                                                                 >  2:390603/1‑241 (MQ=255)                                                                                                 aTCATGAAGGGGGCAATGAGATTCTTGCCGCTTTGAATCCCGGGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGTTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAGCGATTTTGGCCTAATG‑CACGTTGGGGCGGCTCTCAAT‑CGTCCGCTGGTTGCCCTGTATGGTCCGATTAGCCGGTACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGtctg                                                                                                                                                <  1:276746/251‑4 (MQ=255)                                                                                                   cATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATG‑CACGTTGCGGCGGCGCTCAAT‑CGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGAt                                                                                                                                              >  1:112926/1‑251 (MQ=255)                                                                                                    aTGAAGCGGGCAATGAAATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCCCTACCGATTCTGGCCTAATG‑CACCTTGCGGCGGCGCTCAAT‑CGGCCGCTGTTTGCCCTGTATGGTCCGAGTAACCCGGACTTCACACCGCCGCTTTCCCATAAAGCGCGCGTGGTccctctgctt                                                                                                                                             >  2:203205/1‑243 (MQ=255)                                                                                                         gCGGGCAAGGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCAGGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCGGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCGGGCCTAATG‑CACGTTGCGGCGGCGCTCAAT‑CGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCgg                                                                                                                                        <  1:146387/251‑1 (MQ=255)                                                                                                           gggCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATG‑CACGTTGCGGCGGCGCTCAAT‑CGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGGTTACCGGCt                                                                                                                                      >  1:170234/1‑251 (MQ=255)                                                                                                                  gagaTTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGACCCTGGCGGGGGCAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATG‑CACGTTGCGGCGGCGCTCAAT‑CGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCGATcaca                                                                                                                                >  2:43721/1‑250 (MQ=255)                                                                                                                        cTTGCCGCTTTGAATACCGCGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATG‑CACGTTGCGGCGGCGCTCAAT‑CGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCg                                                                                                                         >  1:313254/1‑251 (MQ=255)                                                                                                                            ccGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATG‑CACGTTGCGGCGGCGCTCAAT‑CGTCCGCTGGTTGCCCt                                                                                                                                                                                                                >  2:126380/1‑160 (MQ=255)                                                                                                                             cGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATG‑CACGTTGCGGCGGCGCTCAAT‑CGTTCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCCCACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTaa                                                                                                                      >  2:230276/1‑249 (MQ=255)                                                                                                                                 ttGAATCCCGTGCAGCAGGCAGGGTGTCGGAACCTGGGGGGGAAAACACAGCTGAATCAGGGGGTTATCCTGATTGCAGCCTGAAAAGCCATTGTCACTAACGATTCGGGCATAAGG‑CACGTTGCGGCGGCGCTCAAT‑CGTCCGCTGTTTGCCTTGAATGGTCCGAGTAGCCCGGATTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTg                                                                                                                 <  2:243876/250‑1 (MQ=255)                                                                                                                                 ttGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATG‑CACGTTGCGGCGGCGCTCAAT‑CGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTg                                                                                                                 >  1:104708/1‑250 (MQ=255)                                                                                                                                    aaTACCGCGCAGCAGGCATGGAGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGATATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATG‑CACGTTGCGGCGGCGCTCAAT‑CGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCCTAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGAAAAGGTGACGc                                                                                                             >  2:348449/1‑251 (MQ=255)                                                                                                                                    aaTACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATG‑CACGTTGCGGCGGCGCTCAAT‑CGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCacac                                                                                                                                                                                   >  1:7539/1‑181 (MQ=255)                                                                                                                                    aaTACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATG‑CACGTTGCGGCGGCGCTCAAT‑CGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCacac                                                                                                                                                                                   <  2:7539/181‑1 (MQ=255)                                                                                                                                        ccGAGCAGCAGGAATGGGGTCGGATCCTGGCGGGGGAAACACAGCTTGTTCAAGCGGTTATCCTGATGGCAGCCTGTAAAGCCATTGTCACTAACGTTGCTGGCCTAATG‑ACCGTTGCGGCGGCGCTCAAC‑CGCCCGCTGGTGGCCTGGTATGGTCGGAGCAGCCTGGACTTCACCCCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCg                                                                                                         <  1:281396/251‑1 (MQ=255)                                                                                                                                               gcggcgaTGGTGTCGGAGCCGAGCGTGGAAAACACAGCTTGACGAAGCGGTTACCCTGTTTGCAGCCTGTAAAGCAGTTGCTGCTAACGATTCTGGCCGAAGG‑CCCGTTCCGGCGGGGCAAAAT‑CGCCCGCGGGTTGACCTGTATGGTTCGGGAATTCCGGTCTTCATTCCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGTCGCTGCTGAGGGtt                                                                                                  <  1:61486/245‑1 (MQ=255)                                                                                                                                                   gcagggTGTCGAAACCTGTCGGGGAAAACACAGCTTGATCAGGGTGTTACCCTGATTGTAGCGTGAAAACCAATTGCCACTAACGACTCTGGC‑TTATGGCGCGTTGCGGCGGCGCT‑AATGCGCCCGCTGGTTCCCCTGTATGTTCCGAGGAGCCCGTACTACACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCa                                                                                              <  2:75302/247‑1 (MQ=255)                                                                                                                                                      ggttgccGGAACCGGGCGGGGGAACCCCGTCTTGACCACGCGTTTCTCCGGATGGCGGCCTGTAAAGCCATGGTCCCGAAGGATTCTGGCCTAATG‑CACGTTGCGGCGGCGCTCATC‑CGCCCCCTGGTTGCCCTGTATGGCCCGAGTGGCCCGTACTGCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGTCGATCACAAAGTGCGTAAAGGTGACGCTTCGGAGGGTTATCAcc                                                                                            <  2:277788/244‑1 (MQ=255)                                                                                                                                                            cGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATG‑CACGTTGCGGCGGCGCTCAAT‑CGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCtt                                                                                     >  1:315204/1‑251 (MQ=255)                                                                                                                                                                 ccTGGCGGGGGAAACACAGCTTGATCAAACGGTTTTCCTGATTGCAGCCTGTAAAGCCATTGTCACTAAAGATTCTGGCCTAATG‑CCCGTTGCGGCGGCGCTCAAT‑CGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGACCGGACTTCCCAACGCCGCTATCCCATAAAGCGCGCGTGATCCGTTCAATTCCCGGCTATCACAAAATGCGTAAAGGGGGCGCTGCGGAGGGTTATCACCAGGGCTTGATCg                                                                                >  2:209574/1‑251 (MQ=255)                                                                                                                                                                              acacAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATG‑CACGTTGCGGCGGCGCTCAAT‑CGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTcccc                                                                    >  1:37955/1‑250 (MQ=255)                                                                                                                                                                                acaGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATG‑CACGTTGCGGCGGCGCTCAAT‑CGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAgc                                                                 >  1:398355/1‑251 (MQ=255)                                                                                                                                                                                 catcTTGATCAAACGGTTATCCTGATTGCAGCCTGTAACGACATTGTCCCTAACGATTCTGGCCTAATG‑CACGTTGCGGCGGCACACAAT‑CGTCCGCTGGTTTCCCTGTATGGTCCGCATAGCCCGGACTTAACACCGCCGATATACCATAAAACGCGCGAGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGTCGCTGCGGAGGGTTCTCACCAGCGAGTGGTCGACATTACTCCCCAgcg                                                                >  2:416469/4‑251 (MQ=255)                                                                                                                                                                                       aaTCAGGCGGTTATCCTGAGTGCGGCCTGTAAAGCCATGGTCACTAACGACTCTGGCCTAATG‑CACGTTGGGGCGGCGCCCAAG‑CGTCCGCTGTTTGCCCTGTATGGGCCGAGTAGCCGGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGAGCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTAc                                                           <  2:170234/249‑1 (MQ=255)                                                                                                                                                                                         ccAAGCGGTTATCCTGATTGCAGCTTGTAAAGCCATTGTCACTAACGTTTCTGGCCTAATG‑CACGTTGCGGCGGCGCTCAAT‑CGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTgg                                                        <  1:206690/250‑1 (MQ=255)                                                                                                                                                                                           aaGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATG‑CACGTTGCGGCGGCGCTCAAT‑CGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGga                                                       >  1:308962/1‑250 (MQ=255)                                                                                                                                                                                                  tATCCTGCTTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGTCCTAATG‑CACGTTGCGGCGGCGCTCAAT‑CGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGACCGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACACAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGAAGAACTc                                                >  2:283845/1‑250 (MQ=255)                                                                                                                                                                                                       tGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATG‑CACGTTGCGGCGGCGCTCAAT‑CGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAAcgcg                                          >  1:78550/1‑251 (MQ=255)                                                                                                                                                                                                           tGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATG‑CACGTTGCGGCGGCGCTCAAT‑CGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCCTAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAgg                                                                                                                            >  1:229625/1‑164 (MQ=255)                                                                                                                                                                                                                    taaagcatctGTCACTAGCGATTGTGGCTAAATG‑CACGTTGCGGTGGGGCTCACT‑CGCCCGCTGGTCGCCCTGTTGGGTCCGTTTCGCCCTGTCTCCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑aa                             <  2:87539/241‑1 (MQ=255)                                                                                                                                                                                                                    tAAAGCCATTGTCACTAACGATTCTGACCTAATG‑CACGTTGCGGCGGCGCTCAAT‑CGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑aa                             >  2:207815/1‑250 (MQ=255)                                                                                                                                                                                                                     aaaGCCTTTGTCACTAACTGTTCGGTCCTAATG‑CCCGTTGCGGCGGCCCTCAAC‑GGTCCGCGGGTGGCCTGGTATGTTCCGAGTAGCCGGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑Aag                            <  1:429410/250‑1 (MQ=255)                                                                                                                                                                                                                             tGTCACTAACGATTCTGGCCTAATG‑CACGTTGCGGCGGCGCTCAAT‑CGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTAACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTAATGGAAGAACTCAACGCGCTATTGT‑ACTACGAGGAAGCCt                   >  1:68302/1‑251 (MQ=255)                                                                                                                                                                                                                               tCACTAACGATTCTGGCCTAATG‑CACGTTGCGGCGGCGCTCAAT‑CGTCCGATGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACAACGCAGCTCTCCCATAAAGCGCGCGCGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTACCGCCGCGGAGGGTTATCACCAGACCTTGATCGACATCACTCCCCAGCGCGTAATGGAAGAACTCAACGCGCTATTGTTAC‑ACGAGAAAACCTg                  >  1:193809/1‑250 (MQ=255)                                                                                                                                                                                                                                cACTAACGATTCTGGCCTAATG‑CACGTTGCGGCTGCGCTCAAT‑CGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGAGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGGGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGACGAACTCAACGCGCTATTGTTAC‑AAGAGGACGCCTGAc                >  2:102405/1‑251 (MQ=255)                                                                                                                                                                                                                                           cTGGCCTAATG‑CACGTTGCGGCGGCGCTCAAT‑CGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑AAGAGGAAGCCTGACGGATGCGGGtt     >  1:345391/1‑251 (MQ=255)                                                                                                                                                                                                                                              gCCTAATG‑CACGTTGCGGCGGCGCTCAAT‑CGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTCTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTGATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑AAGAGGAAGCCTGACGGATGCGGGTTTTg  >  2:86827/1‑251 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                   GTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCGGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAATACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATG‑CATGTTGCGGCGGCGCTCAAT‑CGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAAAGCGCGCGTGATCCGTTTGATTACCGGCTATCACAAAGTGCGTAAAGGTGACGCTGCGGAGGGTTATCACCAGAGCTTAATCGACATTACTCCCCAGCGCGTACTGGAAGAACTCAACGCGCTATTGTTAC‑AAGAGGAAGCCTGACGGATGCGGGTTTTG  >  NC_000913/3795503‑3795990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑