Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,795,591	G→T	S221S (TCG→TCT)	waaF →	ADP‑heptose‑‑LPS heptosyltransferase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,795,591	0	G	T	100.0%	143.4 / NA	44	S221S (TCG→TCT)	waaF	ADP‑heptose‑‑LPS heptosyltransferase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (22/22);  total (22/22)

CCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCGGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGGCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAA  >  NC_000913/3795344‑3795830                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 ccTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCg                                                                                                                                                                                                                                               >  1:259812/1‑251 (MQ=255)      gACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAg                                                                                                                                                                                                                                           <  2:153735/250‑1 (MQ=255)          aaGGCATTATGCGTACCGCAAAAGATATGCCGCAGCCATTGTTAAGGAAGCAGTTGCAGGTGAGCCCCGATGAACAATCATATACCTGTAATCCATTTTCGCTTTCATCAGAACGTCCGCTGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACCCTACCACTATGCGGAGCTGGCCAAGCCGCTGATTGATGACGGTTATCAGGTGGTTCTGTTTGGATCTGCGaaaacacat                                                                                                                                                                                                                                      >  1:261748/1‑245 (MQ=255)             gCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGaa                                                                                                                                                                                                                                   <  2:106691/251‑1 (MQ=255)             gCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGa                                                                                                                                                                                                                                    >  1:64746/1‑250 (MQ=255)               aTTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGc                                                                                                                                                                                                                                 >  1:81401/1‑251 (MQ=255)                      gtaccgcacaAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGTTGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGAGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAAt                                                                                                                                                                                                                          >  2:437705/6‑251 (MQ=255)                        accgcacaAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATga                                                                                                                                                                                                                        <  1:329660/248‑1 (MQ=255)                        accgcacaAGATCTGCCGCAGCCATTGTTATGGCCGCAGGTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTGTCGCTTTCATCAGAACGTCCGATGAGTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATga                                                                                                                                                                                                                        <  2:247075/248‑1 (MQ=255)                                  atcggCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGcc                                                                                                                                                                                                              <  2:240613/247‑1 (MQ=255)                                      gCCGCAGCAATTGTTATGGCCGCAATTGCAGAAGAGCGAAGGTCAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGAGCGGAGTTTGGTCCGGCAAAACGCTGGCCACCATACCACTATGCGGAGCTGGCAAACCAACTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGTTCATGAAACGGGCAATGAGATTCTTGCCGCtt                                                                                                                                                                                                          >  2:42761/1‑251 (MQ=255)                                                                tGCAGGTGAGCGAAGGTGAAAAATCATATTCCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGgtg                                                                                                                                                                                <  2:123211/250‑1 (MQ=255)                                                                 gCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTc                                                                                                                                                                              <  2:118059/251‑1 (MQ=255)                                                                 gCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTc                                                                                                                                                                              <  1:271173/251‑1 (MQ=255)                                                                                  aaaaaTCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGa                                                                                                                                                              >  1:301109/1‑250 (MQ=255)                                                                                                   aaTCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAg                                                                                                                                             <  1:294559/250‑1 (MQ=255)                                                                                                       aaTTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGtt                                                                                                                                        <  2:61997/251‑1 (MQ=255)                                                                                                        aTTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGtt                                                                                                                                        >  1:373653/1‑250 (MQ=255)                                                                                                           ttCGCTTTCATCAGCACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCACAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATcc                                                                                                                                    >  2:206690/1‑251 (MQ=255)                                                                                                           ttCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAACACGCTGGCCACACTACCACTATGAGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGAGGTTCTGTTTGGCGCTGCGAAAAATCATGAAGCGGACAATGGGATTCTTGCCGCTTTGAAATACGGA‑CAGCACGCATGGGGGAGGGACCTGGCTGGGGTAACACAGCAGGATCATGGGGTTAtac                                                                                                                                    >  2:234898/1‑249 (MQ=255)                                                                                                                tttCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACAGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACCGCTTGATCAAGCGGTTATCCTGAtt                                                                                                                               >  2:335142/1‑251 (MQ=255)                                                                                                                  tcatcaGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCACAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGATATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAACCTGGAGGGGGACACACAGCTTGATAACGCGGTTATCCTGATTGc                                                                                                                             >  2:67907/1‑251 (MQ=255)                                                                                                                      caGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTGTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGc                                                                                                                          <  1:109066/250‑1 (MQ=255)                                                                                                                        gAACGTCCGATGATTGGCTTTTGCCCGGGAGCGGAGATTGGTCCGGCAAAGCGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGCTTGATGCAGGTTATCAGGGGGTTCTGTTTGGCTCTGCGAACGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAACCACAGCTTGATCAAGCGGTTAGCCTGATTGCAGCCTg                                                                                                                       >  2:404670/1‑251 (MQ=255)                                                                                                                               cGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACCGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGcc                                                                                                                >  2:201402/1‑251 (MQ=255)                                                                                                                                                   ggTGCGGAGTTTGGTCCGGCAAAACGTGGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGAGTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATGCTTGCCGCGTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTgg                                                                                            <  2:114211/251‑1 (MQ=255)                                                                                                                                                              tGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATGCac                                                                                  <  1:349110/250‑2 (MQ=255)                                                                                                                                                                gTCCGGCAAAACGCTGGCCACACTCCCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCCGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATGCACGtt                                                                               >  2:216756/1‑251 (MQ=255)                                                                                                                                                                gTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGGTATCAGGGGGTTCTGGTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑GACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATGCACGtt                                                                               <  2:16873/251‑1 (MQ=255)                                                                                                                                                                         aaCGCTGGCCACACTACCACTATGCGGAGCTGGCCAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCt                                                                                                                        >  2:52581/1‑201 (MQ=255)                                                                                                                                                                         aaCGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCt                                                                                                                        <  1:52581/201‑1 (MQ=255)                                                                                                                                                                                gCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATGCACGTTGCGGCGGCGCTCAATc                                                               <  1:109710/251‑1 (MQ=255)                                                                                                                                                                                        aCCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATGCACGTTGCGGCGGCGCTCAATCGTCCGCt                                                        <  2:81401/250‑1 (MQ=255)                                                                                                                                                                                            cgaTGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCGTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATGCACGTTGCGGCGGCTCTCAATCGTCCGCTGGTTg                                                   <  2:258813/249‑1 (MQ=255)                                                                                                                                                                                                     gCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATGCACGTTGCGGCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATg                                          <  1:256423/251‑1 (MQ=255)                                                                                                                                                                                                           aaaGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGAAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGAACAAGCGGTTATCCTGATTGCAGACTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATGCACGTTGCGGCGGCGCTCACTCGTCCTCTGGTTGCCCTTTATgttc                                       >  1:366772/1‑245 (MQ=255)                                                                                                                                                                                                                 gcTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATGCACGTTGCGGCGGCGCTCAATCGTCCGCTGGTTGCACTGTATGGTCCGAGGAGcc                              >  2:331404/1‑251 (MQ=255)                                                                                                                                                                                                                              ggTTATCAGGTGTTTCTGTTTGGCTCTGCGAAAGATCAGGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCGGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATGCACGTTGCGGCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACAcc                 <  1:157634/251‑1 (MQ=255)                                                                                                                                                                                                                              ggTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATGCACGTTGCGGCGGCGCTCAATCGTCCg                                                          <  2:22653/210‑1 (MQ=255)                                                                                                                                                                                                                              ggTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATGCACGTTGCGGCGGCGCTCAATCGTCCg                                                          >  1:22653/1‑210 (MQ=255)                                                                                                                                                                                                                                  aTCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAGCCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATGCACGTTGCGGCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACAccgcc              <  2:299368/250‑1 (MQ=255)                                                                                                                                                                                                                                  aTCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATGCACGTTGCGGCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGGCCGAGTAGCCCGGACTTCACAccgccg             >  1:243876/1‑251 (MQ=255)                                                                                                                                                                                                                                            tCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGACGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGCTCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATGCACGTTGCGGCGGAGCTCCATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCAt    >  1:23009/1‑250 (MQ=255)                                                                                                                                                                                                                                              tGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTAATGCACGTTGCGGCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCCTaa  >  2:117701/1‑250 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 CCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCGGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCCATTGTCACTAACGATTCTGGCCTGATGCATGTTGCGGCGGCGCTCAATCGTCCGCTGGTTGCCCTGTATGGTCCGAGTAGCCCGGACTTCACACCGCCGCTATCCCATAA  >  NC_000913/3795344‑3795830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑