Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,795,480	T→C	G184G (GGT→GGC)	waaF →	ADP‑heptose‑‑LPS heptosyltransferase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,795,480	0	T	C	100.0%	209.0 / NA	60	G184G (GGT→GGC)	waaF	ADP‑heptose‑‑LPS heptosyltransferase 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/33);  total (27/33)

CCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCGGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCC  >  NC_000913/3795235‑3795720                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  ccTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAATCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTg                                                                                                                                                                                                                                              <  1:112738/251‑1 (MQ=255)         cACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTg                                                                                                                                                                                                                                      >  1:61997/1‑251 (MQ=255)                 ggCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGt                                                                                                                                                                                                                                <  2:17583/249‑1 (MQ=255)                       gcgAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCg                                                                                                                                                                                                                        <  1:384137/251‑1 (MQ=255)                           gaTGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCa                                                                                                                                                                                                                     >  2:256423/1‑250 (MQ=255)                            aTGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAACGATGCCTGGCCGCTAATGGTGGAAAGCTATGTCGCGCTGGCCTATGCCAAAGGCATTATGCGTACCGCACACGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGCGCGAAGGAGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGCACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGcca                                                                                                                                                                                                                    >  1:420421/1‑248 (MQ=255)                            aTGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAATCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCaa                                                                                                                                                                                                                    >  1:123211/1‑250 (MQ=255)                                   aCGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTg                                                                                                                                                                                                             <  2:117406/250‑1 (MQ=255)                                     ggTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGcc                                                                                                                                                                                                          >  1:106691/1‑251 (MQ=255)                                          aCTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACt                                                                                                                                                                                                     <  1:361470/251‑1 (MQ=255)                                          aCTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCAGCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACt                                                                                                                                                                                                     <  2:375442/251‑1 (MQ=255)                                                cGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACt                                                                                                                                                                                               <  1:194710/251‑1 (MQ=255)                                                 gATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGACAAATCATATACCTGTCATCAATTTTCGCTTTCATCAGACCGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGCCCGGCAAACCGCTGGCCACACTCCCACtt                                                                                                                                                                                              >  2:157634/1‑250 (MQ=255)                                                  atgtccgcgtgatgtaTAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGAGCGGGCCTATGACAAAGGCATTAGGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCACAGTTGCAGGTTAGCGAAGGTGAAAAATCATATACCGGTAATCAATTTTCGTTTGCATCAGAACGTCCGATGATTGGCGTTGGCCCGGGTGCGGATTTTGGTCCGGCAAAACGCTGGCCACACGAccccttt                                                                                                                                                                                             <  2:306783/236‑6 (MQ=255)                                                    gTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAAGGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTTAGCGAAGGTGAAAAATCATATACCTGTAAGCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATg                                                                                                                                                                                            <  1:176747/250‑1 (MQ=255)                                                      acgcaagCTCGATCAAGAAGCCTGGCCGCGCATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCGTTGTTATGGCCGAAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTACGGAAAAACGCTGGCCACAATACCACtcagcgg                                                                                                                                                                                         >  2:336361/7‑245 (MQ=255)                                                      aCGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCgg                                                                                                                                                                                         >  1:369772/1‑251 (MQ=255)                                                               cGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCa                                                                                                                                                                                 >  1:87539/1‑250 (MQ=255)                                                                gATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCaaa                                                                                                                                                                               <  1:313095/251‑1 (MQ=255)                                                                gATAAAGAAGCCTGGCAGCTAATGGTGGAACGCTCTGTGGCGCGGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCGGCAGTTGCAGGTGAGCGAAGGTGAAAAATCAGATACCTGTAATCAATTTGCTCTTTCATCAGCACGGGCGATGACTGGCTTTTGCCCGGGTGCGGAGGTTGGTCCGGCAAAACGCTGGGCACGCGGCCACTATGCTGAGCTGGCaaa                                                                                                                                                                               <  2:433206/251‑1 (MQ=255)                                                                      gaagCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCt                                                                                                                                                                         >  2:68298/1‑251 (MQ=255)                                                                      gaagCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCt                                                                                                                                                                         <  1:274038/251‑1 (MQ=255)                                                                       aagCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCtg                                                                                                                                                                        <  1:106513/251‑1 (MQ=255)                                                                         gCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAATATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGGGAAAAATCATATACCTATAATCAATTTTCGCTTTCATCATAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATTCGGATCTGGCAAAGCTGCtgat                                                                                                                                                                      <  2:197916/251‑1 (MQ=255)                                                                             ggCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCtgattgat                                                                                                                                                                  <  1:424770/251‑1 (MQ=255)                                                                                     aTGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGCGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGCAGGtt                                                                                                                                                           >  2:311623/1‑250 (MQ=255)                                                                                     aTGGTGGAACGCTATGTCGCGCTGGCCTATAACAAATGCTTTATTCGGACCGCAAAAGATCTGCCGCAGCCATTGTGATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGATCGTCCGAGGATTGGCTTTTGCCCGGTTTCGGAGTGTGGTCCGGCAATACGCTGTCCACACTACCACTATGCGGAGCTGTCAAAGCAGCTGACTGATGACGGTTa                                                                                                                                                          <  2:420421/251‑1 (MQ=255)                                                                                          ggAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAgg                                                                                                                                                     <  1:30332/251‑1 (MQ=255)                                                                                          ggAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTAGGCGTACCGCACAAGATCTGCCGCAGCCATTGTTAGGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTCATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAGACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAg                                                                                                                                                      <  2:373522/250‑1 (MQ=255)                                                                                            aacgatatgtcgagcTGGCCTATGACAAAAGCATTCTGCGTTCCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGGGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGGCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGTCAAAACGCTGGTCACACTACCACTATGGCGAGTTGGCAAAGCAGCTGATTTATGAAGGTTATCAggtt                                                                                                                                                   <  2:255248/238‑2 (MQ=255)                                                                                                          cTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTc                                                                                                                                     >  1:316287/1‑251 (MQ=255)                                                                                                            ggCCTATGACAAATGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGGTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTc                                                                                                                                     <  1:241844/249‑1 (MQ=255)                                                                                                            ggCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGTCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTct                                                                                                                                    <  2:311893/250‑2 (MQ=255)                                                                                                              ccTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCg                                                                                                                                 >  1:259812/1‑251 (MQ=255)                                                                                                                   gACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAg                                                                                                                             <  2:153735/250‑1 (MQ=255)                                                                                                                       aaGGCATTATGCGTACCGCAAAAGATATGCCGCAGCCATTGTTAAGGAAGCAGTTGCAGGTGAGCCCCGATGAACAATCATATACCTGTAATCCATTTTCGCTTTCATCAGAACGTCCGCTGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACCCTACCACTATGCGGAGCTGGCCAAGCCGCTGATTGATGACGGTTATCAGGTGGTTCTGTTTGGATCTGCGaaaacacat                                                                                                                        >  1:261748/1‑245 (MQ=255)                                                                                                                          gCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGaa                                                                                                                     <  2:106691/251‑1 (MQ=255)                                                                                                                          gCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGa                                                                                                                      >  1:64746/1‑250 (MQ=255)                                                                                                                            aTTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGc                                                                                                                   >  1:81401/1‑251 (MQ=255)                                                                                                                                   gtaccgcacaAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGTTGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGAGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAAt                                                                                                            >  2:437705/6‑251 (MQ=255)                                                                                                                                     accgcacaAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATga                                                                                                          <  1:329660/248‑1 (MQ=255)                                                                                                                                     accgcacaAGATCTGCCGCAGCCATTGTTATGGCCGCAGGTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTGTCGCTTTCATCAGAACGTCCGATGAGTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATga                                                                                                          <  2:247075/248‑1 (MQ=255)                                                                                                                                               atcggCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGcc                                                                                                <  2:240613/247‑1 (MQ=255)                                                                                                                                                   gCCGCAGCAATTGTTATGGCCGCAATTGCAGAAGAGCGAAGGTCAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGAGCGGAGTTTGGTCCGGCAAAACGCTGGCCACCATACCACTATGCGGAGCTGGCAAACCAACTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGTTCATGAAACGGGCAATGAGATTCTTGCCGCtt                                                                                            >  2:42761/1‑251 (MQ=255)                                                                                                                                                                             tGCAGGTGAGCGAAGGTGAAAAATCATATTCCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGgtg                                                                  <  2:123211/250‑1 (MQ=255)                                                                                                                                                                              gCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTc                                                                <  2:118059/251‑1 (MQ=255)                                                                                                                                                                              gCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTc                                                                <  1:271173/251‑1 (MQ=255)                                                                                                                                                                                               aaaaaTCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGa                                                >  1:301109/1‑250 (MQ=255)                                                                                                                                                                                                        ataCCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGATTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCtgattg                                                                                                                                                                    <  2:284595/126‑1 (MQ=255)                                                                                                                                                                                                        ataCCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGAAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCtgattg                                                                                                                                                                    >  1:284595/1‑126 (MQ=255)                                                                                                                                                                                                                aaTCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAg                               <  1:294559/250‑1 (MQ=255)                                                                                                                                                                                                                    aaTTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGtt                          <  2:61997/251‑1 (MQ=255)                                                                                                                                                                                                                     aTTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGtt                          >  1:373653/1‑250 (MQ=255)                                                                                                                                                                                                                        ttCGCTTTCATCAGCACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCACAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATcc                      >  2:206690/1‑251 (MQ=255)                                                                                                                                                                                                                        ttCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAACACGCTGGCCACACTACCACTATGAGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGAGGTTCTGTTTGGCGCTGCGAAAAATCATGAAGCGGACAATGGGATTCTTGCCGCTTTGAAATACGGA‑CAGCACGCATGGGGGAGGGACCTGGCTGGGGTAACACAGCAGGATCATGGGGTTAtac                      >  2:234898/1‑249 (MQ=255)                                                                                                                                                                                                                             tttCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACAGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACCGCTTGATCAAGCGGTTATCCTGAtt                 >  2:335142/1‑251 (MQ=255)                                                                                                                                                                                                                               tcatcaGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCACAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGATATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAACCTGGAGGGGGACACACAGCTTGATAACGCGGTTATCCTGATTGc               >  2:67907/1‑251 (MQ=255)                                                                                                                                                                                                                                   caGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTGTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGc            <  1:109066/250‑1 (MQ=255)                                                                                                                                                                                                                                     gAACGTCCGATGATTGGCTTTTGCCCGGGAGCGGAGATTGGTCCGGCAAAGCGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGCTTGATGCAGGTTATCAGGGGGTTCTGTTTGGCTCTGCGAACGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAACCACAGCTTGATCAAGCGGTTAGCCTGATTGCAGCCTg         >  2:404670/1‑251 (MQ=255)                                                                                                                                                                                                                                            cGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACCGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGcc  >  2:201402/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  CCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCGGCGAAAGATCATGAAGCGGGCAATGAGATTCTTGCCGCTTTGAA‑TACCGAGCAGCAGGCATGGTGTCGGAACCTGGCGGGGGAAACACAGCTTGATCAAGCGGTTATCCTGATTGCAGCCTGTAAAGCC  >  NC_000913/3795235‑3795720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑