Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,795,369	A→C	T147T (ACA→ACC)	waaF →	ADP‑heptose‑‑LPS heptosyltransferase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,795,369	0	A	C	100.0%	245.3 / NA	73	T147T (ACA→ACC)	waaF	ADP‑heptose‑‑LPS heptosyltransferase 2
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (34/39);  total (34/39)

AACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCCAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCGGCGAAAGATCATGAAGC  >  NC_000913/3795131‑3795608                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                aaCGCCGCAAACTGGTTCATAGCCTGCGTGAAAAGCGCTACGGCCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTg                                                                                                                                                                                                                                     <  2:97619/251‑1 (MQ=255)          aacgtggccATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCGTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTTCGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCa                                                                                                                                                                                                                            <  2:245662/243‑1 (MQ=255)                      gcttGCGTGAAAAGCGCTACGGCCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATGAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTAGGACAAAGGCAGTATGCGTCCCGCACAAGATCTGCCGCAGCCATTGTTTTGGCCg                                                                                                                                                                                                                <  2:54079/248‑1 (MQ=255)                       ccTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTCTGTCGCGCTGGCCTATGACAAAGGCATTATGAGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGc                                                                                                                                                                                                               >  1:214657/1‑251 (MQ=255)                             tGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGc                                                                                                                                                                                                         >  1:153735/1‑251 (MQ=255)                                  aGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATTGCCGCAGTTGCAGGTg                                                                                                                                                                                                    >  1:333793/1‑251 (MQ=255)                                     gcTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGc                                                                                                                                                                                                 <  1:98043/251‑1 (MQ=255)                                          gACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCCCCGGCTGGCGCGGCGAGATGCGCTACGGTTTCCTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAgg                                                                                                                                                                                            <  1:329633/251‑1 (MQ=255)                                           aCCGCGCCTACGTCTTACCAAACTCCTTCAAAACTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACGCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGt                                                                                                                                                                                           <  1:17416/251‑1 (MQ=255)                                              acgcCTAAGTCTTACCACACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCCCCGGCTGGCTCGGCGCGATGCGCTCCGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTAGCGCTTGCCTATGACACAGGCATTATGCGTACCGCACCAGATCTGCCTCAGCCATTGTTATGGACGAAGGTGCAGGTGAGCGCAGGTgc                                                                                                                                                                                         >  2:281396/2‑249 (MQ=255)                                                    aCGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACACAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAACAATc                                                                                                                                                                                   >  2:109066/1‑250 (MQ=255)                                                       tCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCatat                                                                                                                                                                               >  2:313095/1‑251 (MQ=255)                                                          tACCAAACGCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGACGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCTAAGGTGAAAAATCAtata                                                                                                                                                                              <  1:73252/249‑1 (MQ=255)                                                           accaaaCTCCGTCAAATCAGCATTAGTGCCTTGCTTCGCGGGTATTCCTCAGCGCCCCGGCTTGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCTTGCTCGATAAAGAAGCCTGGCCGCTAATGGGGGAACGCTATGTCGCGCTGGCCTATTACAAAGGCATGATGCTTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCt                                                                                                                                                                           <  1:268485/247‑1 (MQ=255)                                                               aaCTCCTTCAAATCGGCAGTAGTGCCTTTCGTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCGGGCCTATGACAAAGGCATTATGCGTACCGCACAAGAGCTGCCGCAGCCATTGTTATGGCCGCAGGTGCAGGTGAGCGAAGGTGAAAAAGCATAGACCTGTaa                                                                                                                                                                       <  2:417410/251‑1 (MQ=255)                                                                aCTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGGGAGCGAAGGTGAAAAATCATATACCTGTAAt                                                                                                                                                                      >  2:109710/1‑251 (MQ=255)                                                                           tCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCt                                                                                                                                                           >  2:384137/1‑251 (MQ=255)                                                                           tCTGCATTAGTGCCTTTCTTAGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGAACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCt                                                                                                                                                           >  2:271173/1‑251 (MQ=255)                                                                              gCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTtc                                                                                                                                                        >  2:106513/1‑251 (MQ=255)                                                                                aTTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAACGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTtcat                                                                                                                                                      >  1:220690/1‑251 (MQ=255)                                                                                   aGTGCCTTTCTTCGCGGGTATTCCTCAGCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTtcatca                                                                                                                                                    <  2:333793/250‑1 (MQ=255)                                                                                      gCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGaa                                                                                                                                                 <  2:220569/250‑1 (MQ=255)                                                                                      gCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAAc                                                                                                                                                >  1:247075/1‑251 (MQ=255)                                                                                          ttcttcGCGGGGATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTCTGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGAAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTcc                                                                                                                                            >  2:176747/1‑251 (MQ=255)                                                                                            cttcGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCg                                                                                                                                           >  1:299368/1‑250 (MQ=255)                                                                                            cctcGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTGCATCAGAACGTCCg                                                                                                                                           <  2:411531/248‑1 (MQ=255)                                                                                              tcGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCg                                                                                                                                           <  1:135663/248‑1 (MQ=255)                                                                                                  gggTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTCCTCAACGATGTACGCGTGCTCGATAAAGACGCCTGGCCGCTACTGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTCCCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAACTAATATACATGTCATCACTTTTCGCTTTCATCAGAACGTCCGATGATTg                                                                                                                                    >  1:46438/1‑251 (MQ=255)                                                                                                         ccTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAATCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTg                                                                                                                             <  1:112738/251‑1 (MQ=255)                                                                                                                 cACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTg                                                                                                                     >  1:61997/1‑251 (MQ=255)                                                                                                                         ggCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGt                                                                                                               <  2:17583/249‑1 (MQ=255)                                                                                                                               gcgAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCg                                                                                                       <  1:384137/251‑1 (MQ=255)                                                                                                                                   gaTGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCa                                                                                                    >  2:256423/1‑250 (MQ=255)                                                                                                                                    aTGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAACGATGCCTGGCCGCTAATGGTGGAAAGCTATGTCGCGCTGGCCTATGCCAAAGGCATTATGCGTACCGCACACGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGCGCGAAGGAGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGCACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGcca                                                                                                   >  1:420421/1‑248 (MQ=255)                                                                                                                                    aTGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAATCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCaa                                                                                                   >  1:123211/1‑250 (MQ=255)                                                                                                                                           aCGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTg                                                                                            <  2:117406/250‑1 (MQ=255)                                                                                                                                             ggTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAg                                                                                                                                                                                                  >  1:419405/1‑146 (MQ=255)                                                                                                                                             ggTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAg                                                                                                                                                                                                  <  2:419405/146‑1 (MQ=255)                                                                                                                                             ggTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGcc                                                                                         >  1:106691/1‑251 (MQ=255)                                                                                                                                                  aCTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACt                                                                                    <  1:361470/251‑1 (MQ=255)                                                                                                                                                  aCTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCAGCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACt                                                                                    <  2:375442/251‑1 (MQ=255)                                                                                                                                                        cGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACt                                                                              <  1:194710/251‑1 (MQ=255)                                                                                                                                                         gATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGACAAATCATATACCTGTCATCAATTTTCGCTTTCATCAGACCGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGCCCGGCAAACCGCTGGCCACACTCCCACtt                                                                             >  2:157634/1‑250 (MQ=255)                                                                                                                                                          atgtccgcgtgatgtaTAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGAGCGGGCCTATGACAAAGGCATTAGGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCACAGTTGCAGGTTAGCGAAGGTGAAAAATCATATACCGGTAATCAATTTTCGTTTGCATCAGAACGTCCGATGATTGGCGTTGGCCCGGGTGCGGATTTTGGTCCGGCAAAACGCTGGCCACACGAccccttt                                                                            <  2:306783/236‑6 (MQ=255)                                                                                                                                                            gTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAAGGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTTAGCGAAGGTGAAAAATCATATACCTGTAAGCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATg                                                                           <  1:176747/250‑1 (MQ=255)                                                                                                                                                              acgcaagCTCGATCAAGAAGCCTGGCCGCGCATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCGTTGTTATGGCCGAAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTACGGAAAAACGCTGGCCACAATACCACtcagcgg                                                                        >  2:336361/7‑245 (MQ=255)                                                                                                                                                              aCGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCgg                                                                        >  1:369772/1‑251 (MQ=255)                                                                                                                                                                       cGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCa                                                                >  1:87539/1‑250 (MQ=255)                                                                                                                                                                        gATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCaaa                                                              <  1:313095/251‑1 (MQ=255)                                                                                                                                                                        gATAAAGAAGCCTGGCAGCTAATGGTGGAACGCTCTGTGGCGCGGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCGGCAGTTGCAGGTGAGCGAAGGTGAAAAATCAGATACCTGTAATCAATTTGCTCTTTCATCAGCACGGGCGATGACTGGCTTTTGCCCGGGTGCGGAGGTTGGTCCGGCAAAACGCTGGGCACGCGGCCACTATGCTGAGCTGGCaaa                                                              <  2:433206/251‑1 (MQ=255)                                                                                                                                                                              gaagCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCt                                                        <  1:274038/251‑1 (MQ=255)                                                                                                                                                                              gaagCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCt                                                        >  2:68298/1‑251 (MQ=255)                                                                                                                                                                               aagCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCtg                                                       <  1:106513/251‑1 (MQ=255)                                                                                                                                                                                 gCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAATATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGGGAAAAATCATATACCTATAATCAATTTTCGCTTTCATCATAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATTCGGATCTGGCAAAGCTGCtgat                                                     <  2:197916/251‑1 (MQ=255)                                                                                                                                                                                   cTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACACAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGaaa                                                                                                                                                                                       >  1:254973/1‑119 (MQ=255)                                                                                                                                                                                   cTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGTTGATCGAAGTTGaaa                                                                                                                                                                                       <  2:254973/119‑1 (MQ=255)                                                                                                                                                                                     ggCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACg                                                                                                                                               >  2:97314/1‑157 (MQ=255)                                                                                                                                                                                     ggCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACg                                                                                                                                               <  1:97314/157‑1 (MQ=255)                                                                                                                                                                                     ggCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCtgattgat                                                 <  1:424770/251‑1 (MQ=255)                                                                                                                                                                                             aTGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGCGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGCAGGtt                                          >  2:311623/1‑250 (MQ=255)                                                                                                                                                                                             aTGGTGGAACGCTATGTCGCGCTGGCCTATAACAAATGCTTTATTCGGACCGCAAAAGATCTGCCGCAGCCATTGTGATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGATCGTCCGAGGATTGGCTTTTGCCCGGTTTCGGAGTGTGGTCCGGCAATACGCTGTCCACACTACCACTATGCGGAGCTGTCAAAGCAGCTGACTGATGACGGTTa                                         <  2:420421/251‑1 (MQ=255)                                                                                                                                                                                                  ggAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAgg                                    <  1:30332/251‑1 (MQ=255)                                                                                                                                                                                                  ggAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTAGGCGTACCGCACAAGATCTGCCGCAGCCATTGTTAGGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTCATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAGACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAg                                     <  2:373522/250‑1 (MQ=255)                                                                                                                                                                                                    aacgatatgtcgagcTGGCCTATGACAAAAGCATTCTGCGTTCCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGGGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGGCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGTCAAAACGCTGGTCACACTACCACTATGGCGAGTTGGCAAAGCAGCTGATTTATGAAGGTTATCAggtt                                  <  2:255248/238‑2 (MQ=255)                                                                                                                                                                                                                  cTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTc                    >  1:316287/1‑251 (MQ=255)                                                                                                                                                                                                                    ggCCTATGACAAATGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGGTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTc                    <  1:241844/249‑1 (MQ=255)                                                                                                                                                                                                                    ggCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGTCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTct                   <  2:311893/250‑2 (MQ=255)                                                                                                                                                                                                                      ccTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCg                >  1:259812/1‑251 (MQ=255)                                                                                                                                                                                                                           gACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAg            <  2:153735/250‑1 (MQ=255)                                                                                                                                                                                                                               aaGGCATTATGCGTACCGCAAAAGATATGCCGCAGCCATTGTTAAGGAAGCAGTTGCAGGTGAGCCCCGATGAACAATCATATACCTGTAATCCATTTTCGCTTTCATCAGAACGTCCGCTGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACCCTACCACTATGCGGAGCTGGCCAAGCCGCTGATTGATGACGGTTATCAGGTGGTTCTGTTTGGATCTGCGaaaacacat       >  1:261748/1‑245 (MQ=255)                                                                                                                                                                                                                                  gCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGaa    <  2:106691/251‑1 (MQ=255)                                                                                                                                                                                                                                  gCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGa     >  1:64746/1‑250 (MQ=255)                                                                                                                                                                                                                                    aTTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGCTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCTGCGAAAGATCATGAAGc  >  1:81401/1‑251 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                AACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCCAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATACCTGTAATCAATTTTCGCTTTCATCAGAACGTCCGATGATTGGTTTTTGCCCGGGTGCGGAGTTTGGTCCGGCAAAACGCTGGCCACACTACCACTATGCGGAGCTGGCAAAGCAGCTGATTGATGAAGGTTATCAGGTGGTTCTGTTTGGCTCGGCGAAAGATCATGAAGC  >  NC_000913/3795131‑3795608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑