Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,795,192	C→A	P88P (CCC→CCA)	waaF →	ADP‑heptose‑‑LPS heptosyltransferase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,795,192	0	C	A	100.0%	229.5 / NA	66	P88P (CCC→CCA)	waaF	ADP‑heptose‑‑LPS heptosyltransferase 2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (30/36);  total (30/36)

CGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATTCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCCAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATA  >  NC_000913/3794946‑3795436                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     cGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACt                                                                                                                                                                                                                                                  >  2:93161/1‑251 (MQ=255)  ggCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCGCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTc                                                                                                                                                                                                                                                 <  2:76121/251‑1 (MQ=255)  ggCCCGTCTTGGGTTGGCGACATGATGAAGTCGCAAAGTCTCTACCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTAGCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCTAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCTCGCTTACTTCGTACCAAACTc                                                                                                                                                                                                                                                 <  2:65914/251‑1 (MQ=255)           tGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAAt                                                                                                                                                                                                                                         <  1:34385/250‑1 (MQ=255)                tGGCGACATGATGATGTCGCAAAGTCTCTAGCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTAGCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCa                                                                                                                                                                                                                                    <  2:4531/250‑1 (MQ=255)                       atgatgatgTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAAGCTGCATTAGTGcc                                                                                                                                                                                                                            <  1:318977/251‑1 (MQ=255)                           tgatgTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTttc                                                                                                                                                                                                                        >  2:194710/1‑251 (MQ=255)                            gatgTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTttct                                                                                                                                                                                                                       >  1:346113/1‑251 (MQ=255)                              tgTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAAACGATAATCGATGTGATGGCACCGGCCTGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTAGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGCGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAAATCCTTCAAATCTGCATTACTGCCTttcttc                                                                                                                                                                                                                     >  2:276746/1‑251 (MQ=255)                                      aGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGGGACTTTCTTCGCGGGTAt                                                                                                                                                                                                             >  1:311893/1‑251 (MQ=255)                                                gCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGc                                                                                                                                                                                                   >  1:375442/1‑251 (MQ=255)                                                   cGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCTTTAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCAcc                                                                                                                                                                                                <  1:302839/251‑1 (MQ=255)                                                        cAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTg                                                                                                                                                                                           >  1:193262/1‑251 (MQ=255)                                                          ggCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGCAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTATTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGgc                                                                                                                                                                                         >  1:373522/1‑251 (MQ=255)                                                               gcTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTATTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCgcggc                                                                                                                                                                                    >  1:240613/1‑251 (MQ=255)                                                                cTATCCCCACGCGATAATCGATGTGATGGCACCAGCATGGTGCCGTCAATTCTTATCGCGGATGCCGGAAGCTAAAGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGAAAACTGGGTCATAGCCTGCGTGACAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCgcggcg                                                                                                                                                                                   >  2:30332/1‑251 (MQ=255)                                                                     cccAGGCGATAAGCGATGTGATGGCGCCGGCATGGTGCCGTCAATTATTATCGCGGGTGCCGGAAGGTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCAGCGCACCGGCTTGCGCGGCGGGATg                                                                                                                                                                              <  2:305212/251‑1 (MQ=255)                                                                      ccAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGTAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATgc                                                                                                                                                                             <  1:384868/251‑1 (MQ=255)                                                                           cGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTAc                                                                                                                                                                        >  1:411913/1‑251 (MQ=255)                                                                            gATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCTGCGAGATGCGCTACg                                                                                                                                                                       <  2:49400/251‑1 (MQ=255)                                                                            gATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACg                                                                                                                                                                       <  2:411913/251‑1 (MQ=255)                                                                               aaTCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGtt                                                                                                                                                                    <  1:93519/251‑1 (MQ=255)                                                                               aaTCGATGTGATGGCACCGGCATGGTGCCGGCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGtt                                                                                                                                                                    <  2:193262/251‑1 (MQ=255)                                                                                        gATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACg                                                                                                                                                           <  2:373381/251‑1 (MQ=255)                                                                                                    aTGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGCAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGc                                                                                                                                               >  2:274038/1‑251 (MQ=255)                                                                                                    aTGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGc                                                                                                                                               >  1:3455/1‑251 (MQ=255)                                                                                                          ccGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTAGCCCTATGCCTCGCGGTCACGTAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAACCTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTTCGGTTGACTCAACGATGTACGCGTGCTCGATa                                                                                                                                         <  1:279990/251‑1 (MQ=255)                                                                                                             tCCATTATTATCGCGGCTGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGAGAAAAGCGCTACGACCGCGCCTACGTCTTACCAACCTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGACCCGGCTGGCGCGGCGAGATGCGCAACGGTTTACTCAACGATCTCCGCGTGCTCGATaa                                                                                                                                        >  1:75302/1‑249 (MQ=255)                                                                                                               cATTATTATCGCGGCTGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCCAATCTGCATTCGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTCCTCAACGATGTACGCGTg                                                                                                                                                >  2:192988/1‑239 (MQ=255)                                                                                                               cATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGGCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTg                                                                                                                                                <  1:192988/239‑1 (MQ=255)                                                                                                                attattATCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCGCTGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGTGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTGCGCAGGTATTCCGCATCGCACCGGCGGGCGCGGCGAGATGCGCTACGGTTTACGCAACGACGTACGCTTGCTCGATAtagaag                                                                                                                                   <  2:174683/251‑1 (MQ=255)                                                                                                                  tattatCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCGCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACGCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGc                                                                                                                                  <  1:21798/250‑1 (MQ=255)                                                                                                                   attatCGCGGATGCCGGAAGTTACCGAAGCTATCCCTATGCCTCTCGGTCACGGGGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTCCGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGt                                                                                                                                                                                                               >  2:131905/1‑172 (MQ=255)                                                                                                                   attatCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGt                                                                                                                                                                                                               <  1:131905/172‑1 (MQ=255)                                                                                                                   attatCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCGGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCt                                                                                                                                <  1:378443/251‑1 (MQ=255)                                                                                                                      atCGCGGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGc                                                                                                                             <  1:271855/251‑1 (MQ=255)                                                                                                                        cgcgGATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCg                                                                                                                           <  1:182948/251‑1 (MQ=255)                                                                                                                           ggATGCCGGAAGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCt                                                                                                                         >  2:294559/1‑250 (MQ=255)                                                                                                                                    aaGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGaa                                                                                                               <  2:3455/251‑1 (MQ=255)                                                                                                                                    aaGTTAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGa                                                                                                                >  2:349110/1‑250 (MQ=255)                                                                                                                                        tAACGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTCCTCCACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCt                                                                                                           >  1:258813/1‑251 (MQ=255)                                                                                                                                         agcgagcatcgcccattgcctagcGGTCACGTAGCGCGGAAAATCGGCGACCGCAGCAAAATGTGACATAGCCAGCGTGAAAAGCGCAACGATCGCGCCTACGTTTTATCAAACCCCTTCAATTCTGCATTAGTGCCTTTCTTCGCTGGTATTCCTCATCGCACCGGTTGGCGCGGCGAGATGCGATACGGTTTACTCACCGATGTCCGCGTTCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCt                                                                                                           <  2:354342/227‑1 (MQ=255)                                                                                                                                         aaCGAAGCTAACCATATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACGCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTATTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCt                                                                                                           <  2:58297/250‑1 (MQ=255)                                                                                                                                          aCGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCtat                                                                                                         <  2:346113/251‑1 (MQ=255)                                                                                                                                           cGAAGCTATCCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCtatg                                                                                                        >  2:361470/1‑250 (MQ=255)                                                                                                                                                 tatcccTATGCATCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCtatgtcgcgc                                                                                                  >  2:146387/5‑244 (MQ=255)                                                                                                                                                      cTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGcc                                                                                             >  1:118059/1‑251 (MQ=255)                                                                                                                                                        aTGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGcc                                                                                             <  1:93161/249‑1 (MQ=255)                                                                                                                                                          gCCTCTCGGTCACGGAGGGATGGAAAGCGGCGACCGCCGCAAACTGGGTCATAGCCTGCGGGAAAAGCGCTACGACCGCGCCTACGTCTTACCACACTCCTTCAAATCTGCATGAGTGCCTGTCTGCGCGGGTATTCCTCAGCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGACCGCTATGTCGCGCTGGCCTAt                                                                                          <  2:4935/250‑1 (MQ=255)                                                                                                                                                                cGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACaa                                                                                     >  1:16873/1‑249 (MQ=255)                                                                                                                                                                      cGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCAtt                                                                              >  1:411531/1‑250 (MQ=255)                                                                                                                                                                       ggAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTAt                                                                            <  2:436866/251‑1 (MQ=255)                                                                                                                                                                                   aTCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCtatgt                                                                                                       <  2:353090/212‑3 (MQ=255)                                                                                                                                                                                          aaCGCCGCAAACTGGTTCATAGCCTGCGTGAAAAGCGCTACGGCCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTg                                                         <  2:97619/251‑1 (MQ=255)                                                                                                                                                                                                   aacgtggccATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCGTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTTCGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCa                                                <  2:245662/243‑1 (MQ=255)                                                                                                                                                                                                               gcttGCGTGAAAAGCGCTACGGCCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATGAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTAGGACAAAGGCAGTATGCGTCCCGCACAAGATCTGCCGCAGCCATTGTTTTGGCCg                                    <  2:54079/248‑1 (MQ=255)                                                                                                                                                                                                                ccTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTCTGTCGCGCTGGCCTATGACAAAGGCATTATGAGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGc                                   >  1:214657/1‑251 (MQ=255)                                                                                                                                                                                                                      tGAAAAGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGc                             >  1:153735/1‑251 (MQ=255)                                                                                                                                                                                                                           aGCGCTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATTGCCGCAGTTGCAGGTg                        >  1:333793/1‑251 (MQ=255)                                                                                                                                                                                                                              gcTACGACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGc                     <  1:98043/251‑1 (MQ=255)                                                                                                                                                                                                                                   gACCGCGCCTACGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCCCCGGCTGGCGCGGCGAGATGCGCTACGGTTTCCTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAgg                <  1:329633/251‑1 (MQ=255)                                                                                                                                                                                                                                    aCCGCGCCTACGTCTTACCAAACTCCTTCAAAACTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACGCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGt               <  1:17416/251‑1 (MQ=255)                                                                                                                                                                                                                                       acgcCTAAGTCTTACCACACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCCCCGGCTGGCTCGGCGCGATGCGCTCCGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTAGCGCTTGCCTATGACACAGGCATTATGCGTACCGCACCAGATCTGCCTCAGCCATTGTTATGGACGAAGGTGCAGGTGAGCGCAGGTgc             >  2:281396/2‑249 (MQ=255)                                                                                                                                                                                                                                             aCGTCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACACAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAACAATc       >  2:109066/1‑250 (MQ=255)                                                                                                                                                                                                                                                tCTTACCAAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCatat   >  2:313095/1‑251 (MQ=255)                                                                                                                                                                                                                                                   tACCAAACGCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGACGCCTGGCCGCTAATGGTGGAACGCTATGTCGCGCTGGCCTATGACAAAGGCATTATGCGTACCGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCTAAGGTGAAAAATCAtata  <  1:73252/249‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     CGGCCCGTCTTGGGTTGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTCCAGGCGCGCTATCCCCAGGCGATAATCGATGTGATGGCACCGGCATGGTGCCGTCCATTATTATCGCGGATGCCGGAAGTTAACGAAGCTATTCCTATGCCTCTCGGTCACGGAGCGCTGGAAATCGGCGAACGCCGCAAACTGGGTCATAGCCTGCGTGAAAAGCGCTACGACCGCGCCTACGTCTTACCCAACTCCTTCAAATCTGCATTAGTGCCTTTCTTCGCGGGTATTCCTCATCGCACCGGCTGGCGCGGCGAGATGCGCTACGGTTTACTCAACGATGTACGCGTGCTCGATAAAGAAGCCTGGCCGCTAATGGTGGAACGCTATATAGCGCTGGCCTATGACAAAGGCATTATGCGCACAGCACAAGATCTGCCGCAGCCATTGTTATGGCCGCAGTTGCAGGTGAGCGAAGGTGAAAAATCATATA  >  NC_000913/3794946‑3795436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑