Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,794,337	A→G	A117A (GCA→GCG)	rfaD →	ADP‑L‑glycero‑D‑mannoheptose 6‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,794,337	0	A	G	100.0%	189.5 / NA	56	A117A (GCA→GCG)	rfaD	ADP‑L‑glycero‑D‑mannoheptose 6‑epimerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/29);  total (27/29)

GGCACCAAGTTTGTGAACCTGGTGGATCTGAATATCGCAGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGTGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCAACGTTTATGGACCGCGTGAAGGCCATAAAGGCAGCATGGCGAGCGTCGCTTTCCATCTCAACACCCAGCTTAACAACGGTGAATCACCGAAG  >  NC_000913/3794092‑3794583                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       ggCACCAAGTTTGTGAACCTGGTGGATCTGGATATCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCAc                                                                                                                                                                                                                                                   >  2:330023/1‑251 (MQ=255)   cACCAAGTTTGTGAACCTGGTGGATCTGGATATCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTGCCACGAAGGCGCATGCTCTTCCACCAGCGAGGGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCGGTTCCTGTATGCAGCGTCCGCGGCCACCt                                                                                                                                                                                                                                                 <  1:146211/251‑1 (MQ=255)       aaGTTTGTGAACCTGGTGGATATGGATATCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCAACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGAGCGAAATCCCGTTCATGTAGGCATCTTACGCGGCCACCTAcgg                                                                                                                                                                                                                                             >  1:165330/1‑251 (MQ=255)            tgtgAACCTGGTGGATCTGGATATCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGAc                                                                                                                                                                                                                                        >  1:256254/1‑251 (MQ=255)                  ccTGGTGGATCTGGATATCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCAccc                                                                                                                                                                                                                                  >  1:71251/1‑250 (MQ=255)                     ggtggATCTGGATATCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCg                                                                                                                                                                                                                               >  1:108466/1‑251 (MQ=255)                              ggatatCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTAt                                                                                                                                                                                                                        <  2:103130/247‑1 (MQ=255)                              ggatatCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGGCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCGCCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTAGCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAACCCCGTTCCTGGATGCATCTTACGCGGCCACCTACGGCGGACGCATCTCCGCCtt                                                                                                                                                                                                                           <  2:385571/244‑1 (MQ=255)                              ggatatCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGAAGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATTGCAAGTATATGATGGATAACAACTATCATTACTCCAAAGAGCTATTGCACTACTGTCTGGAGCGCGAAATACCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCAGACGCACCTCCGACTTTATTg                                                                                                                                                                                                                      >  1:81556/3‑251 (MQ=255)                                 tatCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGCCGCACCCCCGGCttt                                                                                                                                                                                                                          >  2:207623/1‑244 (MQ=255)                                 tatCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACttt                                                                                                                                                                                                                          <  1:207623/244‑1 (MQ=255)                                            aaatGGATAAGGAAGACGTCCTGATCCAGATTAGGGCTGGCGAAGAGTGCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTACACGACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGt                                                                                                                                                                                                          <  2:81556/247‑1 (MQ=255)                                                       gAAGATTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAACGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGACAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTACTGCACTACTGTCTGGAGCGCGAACTCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCCACTTTATTGAATTCCGCGAGTAAGAAAAACCGtt                                                                                                                                                                                             >  1:51677/1‑251 (MQ=255)                                                                 tGATCCAGATTATGGCTGGCGAAGAGTTCGGCGCTGTCGAAGCGCTTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTTTTTAATCCCGCGGTTACGAACAACCGTTGAACGTCTAc                                                                                                                                                                                   >  2:138722/1‑251 (MQ=255)                                                                  gATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACg                                                                                                                                                                                  <  2:384328/251‑1 (MQ=255)                                                                        gATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGGGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGAATTTTTTTAATCCCGCGGGGACGCAAAAACGTTTAACGTCTACGGTTACt                                                                                                                                                                            >  2:239518/1‑251 (MQ=255)                                                                           tATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGCCTTTATTGAATCCCGCGCGTACGAAAAACCGTTTAACGTCTACGGTTACTCa                                                                                                                                                                          >  2:251807/1‑250 (MQ=255)                                                                            aTGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCaaa                                                                                                                                                                        <  1:301444/251‑1 (MQ=255)                                                                               gctggcGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATACCAACTATAAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGTATCCCGCGAGGACGAAAAACCGATGAACGTATACGGGTACTCAAAAtt                                                                                                                                                                     >  1:282498/1‑251 (MQ=255)                                                                                   gcGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTg                                                                                                                                                                 <  2:238543/251‑1 (MQ=255)                                                                                   gcGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTg                                                                                                                                                                 <  1:435384/251‑1 (MQ=255)                                                                                                      cGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGt                                                                                                                                               <  1:251807/250‑1 (MQ=255)                                                                                                       gAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATCCTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGCCTTTATTTAATCCCGCGAGTACGAAAAACCGGTGAACGTCCCCGGTTACTCAAAATTCCTGTTTGCTGAATATGTTCGTc                                                                                                                                              >  2:385/1‑250 (MQ=255)                                                                                                          gCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAAt                                                                                                                                          >  2:144672/1‑251 (MQ=255)                                                                                                          gCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAAt                                                                                                                                          <  1:289460/251‑1 (MQ=255)                                                                                                               tttCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGCTGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGCTGAATATGTTCGTCAAATcctgc                                                                                                                                     >  2:63012/1‑251 (MQ=255)                                                                                                               tttCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATcctg                                                                                                                                      <  2:14012/250‑1 (MQ=255)                                                                                                                  ccACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTTGAGCGCGAAATCCCGTTCCTGGATGCATCTTCCGCGGTCACCTACGGCGGACGCCCCTCCGACTTTAGTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTTCGGGTACGCAAAAGTCCTGTTTCATGAATATGTTCGTCAAATcctgcct                                                                                                                                   <  2:189585/250‑1 (MQ=255)                                                                                                                  ccACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTGTGATGAATATGTTCGTCAAAGcctgcctg                                                                                                                                  <  2:306845/251‑1 (MQ=255)                                                                                                                                     tCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAAGCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATGCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACGCGCAGAttg                                                                                                                <  2:220194/250‑1 (MQ=255)                                                                                                                                     tCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTGATTGAATCCCGCTAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAAGGCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGAt                                                                                                                  <  1:34762/248‑1 (MQ=255)                                                                                                                                         ccaccGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGc                                                                                                           >  1:253481/1‑251 (MQ=255)                                                                                                                                             cGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGCAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGCCGCACCTCCGACTTTCTTGAATCCCGCGCGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGGCAAATCCTGCCTGAAGAGCACTCGCAGATTGTTCGCTTcc                                                                                                       >  2:353709/1‑251 (MQ=255)                                                                                                                                             cGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTcc                                                                                                       <  1:315143/251‑1 (MQ=255)                                                                                                                                                       ggCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCa                                                                                              >  1:380609/1‑250 (MQ=255)                                                                                                                                                       ggCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCa                                                                                              >  2:14614/1‑250 (MQ=255)                                                                                                                                                            gTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCAACGttt                                                                                        <  1:394800/251‑1 (MQ=255)                                                                                                                                                              atctgatgGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAAGCCCGTTCCTGTATGCATCTTCCGCGGCCTCCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGATCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCAACGTTTa                                                                                       <  2:245934/247‑1 (MQ=255)                                                                                                                                                                       aTAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTTAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCAACTTTTATGGACCGCGt                                                                             <  1:59596/251‑1 (MQ=255)                                                                                                                                                                             acTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCAACGTTTATGGACCGCGTGAAGGc                                                                       <  1:209972/251‑1 (MQ=255)                                                                                                                                                                                aTCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAAGCCTGCCTGAAGCGAACTCGCAGAGTGTGGGCTGCCGGTAGTTCAACGTTTATGGACCGCGTGAAGGcc                                                                      <  2:280302/249‑1 (MQ=255)                                                                                                                                                                                      aCTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATc                                                                                                                                                                                                                  <  2:22142/103‑1 (MQ=255)                                                                                                                                                                                      aCTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATc                                                                                                                                                                                                                  >  1:22142/1‑103 (MQ=255)                                                                                                                                                                                        tCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCCTCTTCCGCGGCCCCCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGACAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCAACGTTTATGGACCGCGTGAAGGCCATAAAGgc                                                              >  2:334961/1‑249 (MQ=255)                                                                                                                                                                                            aagAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCAACGTTTATGGACCGCGTGAAGGCCATAAAGGCAGCAtt                                                        <  2:14095/249‑2 (MQ=255)                                                                                                                                                                                            aagAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCAACGTTTATGGACCGCGTGAAGGCCATAAAGGCAGCATg                                                        >  2:313833/3‑251 (MQ=255)                                                                                                                                                                                               aGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTcc                                                                                                       <  1:222783/201‑1 (MQ=255)                                                                                                                                                                                                   gctgcACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCAACGTTTATGGACCGCGTGAAGGCCATAAAGGCAGCATGGCGAGCg                                                 >  2:166901/1‑251 (MQ=255)                                                                                                                                                                                                           actGTCTGGAGCGCGAAATAACGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGCGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGCCAAATCCTGCCTGAAGCGCAATCGCAGATTGTTGGCTTCCGTTCTTTACACGTATATGGACAGCGTGAAGGCCATAAAGGCAGCATGGCGTGCGGCGCTTTc                                         >  2:93519/1‑251 (MQ=255)                                                                                                                                                                                                             tgtctggagagcgacaTCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCAACGTTTATGGACCGCGTGAAGGCCATAAAGGCAGCATGGCGAGCGTCGCTTTcc                                        >  1:139639/16‑250 (MQ=255)                                                                                                                                                                                                                  ggAGCGCGAAATCCCGTTCCTGGATGCATCTTCAGCGGACACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGGTACTCAAACTTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTTGCAGATTGTTGGCTTCCGTTATTTCAACGTTTATGGACCGCGTGAAGGCCATAAAGGCAGCATTGCGAGCGTCGCTTTCCAtctc                                   >  2:296847/1‑250 (MQ=255)                                                                                                                                                                                                                     gcgcgAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCAACGTTTATGGACCGCGTGAAGGCCATAAAGGCAGCATGGCGAGCGTCGCTTTCCATCTCAaca                               <  1:239518/247‑1 (MQ=255)                                                                                                                                                                                                                         gAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGGCAAAGCCTGCGTGAAGCGAGCTCGCATATTGTTGGCTTGCGTTAGTTCAACGTTTATGGACCGCGTGAAGGCCATAAAGGCAGCATGGCGAGCGTCGCTTTCCATCTCAACACCCa                           <  1:252257/251‑1 (MQ=255)                                                                                                                                                                                                                               ccGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCAACGTTTATGGACCGCGTGAAGGCCATAAAGGCAGCATGGCGAGCGTCGCTTTCCATCTCAACACCCAGCTTaa                     <  1:63012/251‑1 (MQ=255)                                                                                                                                                                                                                                                  ccGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGCCTCCCGCGAGTACGACAAACCGTTGAACGTCTACGGTTACTCAAAATTACTGTTTGCTGACTATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGTTTGTTGGCTTCCGTTATTTCAACGTTTATGGACCGCGTGAAGGCCATAAAGGCAGCATGGCGAGCGTCGCTTTCCATCTCACCACCCCGCTTAACAACGGTGAATCCCCGaac  >  2:273416/1‑250 (MQ=255)                                                                                                                                                                                                                                                  ccGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCAACGTTTATGGACCGCGTGAAGGCCATAAAGGCAGCATGGCGAGCGTCGCTTTCCATCTCAACACCCAGCTTAACAACGGTGAATCACCGAAg  <  1:4637/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       GGCACCAAGTTTGTGAACCTGGTGGATCTGAATATCGCAGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGTGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCAACGTTTATGGACCGCGTGAAGGCCATAAAGGCAGCATGGCGAGCGTCGCTTTCCATCTCAACACCCAGCTTAACAACGGTGAATCACCGAAG  >  NC_000913/3794092‑3794583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑