Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,794,307	T→C	R107R (CGT→CGC)	rfaD →	ADP‑L‑glycero‑D‑mannoheptose 6‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,794,307	0	T	C	100.0%	182.0 / NA	54	R107R (CGT→CGC)	rfaD	ADP‑L‑glycero‑D‑mannoheptose 6‑epimerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/27);  total (27/27)

CGATATTCTGGTGGTGGACAACCTGAAAGACGGCACCAAGTTTGTGAACCTGGTGGATCTGAATATCGCAGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGTGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCAACGTTTATGGACCGCGTGAAGGCCATAAAGGCAGCATGGCGAGCGTCGCTTTCCATCTC  >  NC_000913/3794061‑3794550                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    cGATATTCTGGTGGTGGACAACCTGAAAGACGGCACCAAGTTTGTGAACCTGGTGGATCTGGATATCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGaa                                                                                                                                                                                                                                                  >  2:209972/1‑250 (MQ=255)  gATATTCTGGTGGTGGACAACCTGAAAGACGGCAACAAGTTTGTGAACCTGGTGGATCTGGATATCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAAt                                                                                                                                                                                                                                                <  2:436916/251‑1 (MQ=255)                ggACAACCACAAAGCCAGCACCACCTTTGTGACCCTGGTGGTTCTCGATCTCGCGGACTATATCGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATTTCGAAGCGATTTTCCACGAAGCCGCATGCTCTTCCACCACCGAGAGGGATGTAAAGTATATGATGGGAAACACCTATCAATACTCCAAACACCTGCTGCAATACTGTCTGGAGCACGAAATCCCATTCCTGTa                                                                                                                                                                                                                                    >  2:146211/1‑249 (MQ=255)                             gACGGCACCAAGTTTGTGAACCTGGTGGATCTGGATATCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGcggc                                                                                                                                                                                                                     <  1:171138/251‑4 (MQ=255)                                ggCACCAAGTTTGTGAACCTGGTGGATCTGGATATCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCAc                                                                                                                                                                                                                  >  2:330023/1‑251 (MQ=255)                                  cACCAAGTTTGTGAACCTGGTGGATCTGGATATCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTGCCACGAAGGCGCATGCTCTTCCACCAGCGAGGGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCGGTTCCTGTATGCAGCGTCCGCGGCCACCt                                                                                                                                                                                                                <  1:146211/251‑1 (MQ=255)                                      aaGTTTGTGAACCTGGTGGATATGGATATCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCAACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGAGCGAAATCCCGTTCATGTAGGCATCTTACGCGGCCACCTAcgg                                                                                                                                                                                                            >  1:165330/1‑251 (MQ=255)                                           tgtgAACCTGGTGGATCTGGATATCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGAc                                                                                                                                                                                                       >  1:256254/1‑251 (MQ=255)                                                 ccTGGTGGATCTGGATATCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCAccc                                                                                                                                                                                                 >  1:71251/1‑250 (MQ=255)                                                    ggtggATCTGGATATCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCg                                                                                                                                                                                              >  1:108466/1‑251 (MQ=255)                                                             ggatatCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTAt                                                                                                                                                                                       <  2:103130/247‑1 (MQ=255)                                                             ggatatCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGGCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCGCCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTAGCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAACCCCGTTCCTGGATGCATCTTACGCGGCCACCTACGGCGGACGCATCTCCGCCtt                                                                                                                                                                                          <  2:385571/244‑1 (MQ=255)                                                             ggatatCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGAAGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATTGCAAGTATATGATGGATAACAACTATCATTACTCCAAAGAGCTATTGCACTACTGTCTGGAGCGCGAAATACCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCAGACGCACCTCCGACTTTATTg                                                                                                                                                                                     >  1:81556/3‑251 (MQ=255)                                                                tatCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGCCGCACCCCCGGCttt                                                                                                                                                                                         >  2:207623/1‑244 (MQ=255)                                                                tatCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACttt                                                                                                                                                                                         <  1:207623/244‑1 (MQ=255)                                                                           aaatGGATAAGGAAGACGTCCTGATCCAGATTAGGGCTGGCGAAGAGTGCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTACACGACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGt                                                                                                                                                                         <  2:81556/247‑1 (MQ=255)                                                                                      gAAGATTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAACGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGACAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTACTGCACTACTGTCTGGAGCGCGAACTCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCCACTTTATTGAATTCCGCGAGTAAGAAAAACCGtt                                                                                                                                                            >  1:51677/1‑251 (MQ=255)                                                                                                tGATCCAGATTATGGCTGGCGAAGAGTTCGGCGCTGTCGAAGCGCTTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTTTTTAATCCCGCGGTTACGAACAACCGTTGAACGTCTAc                                                                                                                                                  >  2:138722/1‑251 (MQ=255)                                                                                                 gATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACg                                                                                                                                                 <  2:384328/251‑1 (MQ=255)                                                                                                       gATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGGGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGAATTTTTTTAATCCCGCGGGGACGCAAAAACGTTTAACGTCTACGGTTACt                                                                                                                                           >  2:239518/1‑251 (MQ=255)                                                                                                          tATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGCCTTTATTGAATCCCGCGCGTACGAAAAACCGTTTAACGTCTACGGTTACTCa                                                                                                                                         >  2:251807/1‑250 (MQ=255)                                                                                                           aTGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCaaa                                                                                                                                       <  1:301444/251‑1 (MQ=255)                                                                                                              gctggcGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATACCAACTATAAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGTATCCCGCGAGGACGAAAAACCGATGAACGTATACGGGTACTCAAAAtt                                                                                                                                    >  1:282498/1‑251 (MQ=255)                                                                                                                  gcGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTg                                                                                                                                <  2:238543/251‑1 (MQ=255)                                                                                                                  gcGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTg                                                                                                                                <  1:435384/251‑1 (MQ=255)                                                                                                                                     cGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGt                                                                                                              <  1:251807/250‑1 (MQ=255)                                                                                                                                      gAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATCCTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGCCTTTATTTAATCCCGCGAGTACGAAAAACCGGTGAACGTCCCCGGTTACTCAAAATTCCTGTTTGCTGAATATGTTCGTc                                                                                                             >  2:385/1‑250 (MQ=255)                                                                                                                                         gCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAAt                                                                                                         >  2:144672/1‑251 (MQ=255)                                                                                                                                         gCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAAt                                                                                                         <  1:289460/251‑1 (MQ=255)                                                                                                                                              tttCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGCTGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGCTGAATATGTTCGTCAAATcctgc                                                                                                    >  2:63012/1‑251 (MQ=255)                                                                                                                                              tttCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATcctg                                                                                                     <  2:14012/250‑1 (MQ=255)                                                                                                                                                 ccACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTTGAGCGCGAAATCCCGTTCCTGGATGCATCTTCCGCGGTCACCTACGGCGGACGCCCCTCCGACTTTAGTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTTCGGGTACGCAAAAGTCCTGTTTCATGAATATGTTCGTCAAATcctgcct                                                                                                  <  2:189585/250‑1 (MQ=255)                                                                                                                                                 ccACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTGTGATGAATATGTTCGTCAAAGcctgcctg                                                                                                 <  2:306845/251‑1 (MQ=255)                                                                                                                                                                    tCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAAGCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATGCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACGCGCAGAttg                                                                               <  2:220194/250‑1 (MQ=255)                                                                                                                                                                    tCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTGATTGAATCCCGCTAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAAGGCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGAt                                                                                 <  1:34762/248‑1 (MQ=255)                                                                                                                                                                        ccaccGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGc                                                                          >  1:253481/1‑251 (MQ=255)                                                                                                                                                                            cGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGCAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGCCGCACCTCCGACTTTCTTGAATCCCGCGCGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGGCAAATCCTGCCTGAAGAGCACTCGCAGATTGTTCGCTTcc                                                                      >  2:353709/1‑251 (MQ=255)                                                                                                                                                                            cGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTcc                                                                      <  1:315143/251‑1 (MQ=255)                                                                                                                                                                                      ggCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCa                                                             >  1:380609/1‑250 (MQ=255)                                                                                                                                                                                      ggCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCa                                                             >  2:14614/1‑250 (MQ=255)                                                                                                                                                                                           gTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCAACGttt                                                       <  1:394800/251‑1 (MQ=255)                                                                                                                                                                                             atctgatgGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAAGCCCGTTCCTGTATGCATCTTCCGCGGCCTCCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGATCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCAACGTTTa                                                      <  2:245934/247‑1 (MQ=255)                                                                                                                                                                                                      aTAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTTAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCAACTTTTATGGACCGCGt                                            <  1:59596/251‑1 (MQ=255)                                                                                                                                                                                                            acTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCAACGTTTATGGACCGCGTGAAGGc                                      <  1:209972/251‑1 (MQ=255)                                                                                                                                                                                                               aTCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAAGCCTGCCTGAAGCGAACTCGCAGAGTGTGGGCTGCCGGTAGTTCAACGTTTATGGACCGCGTGAAGGcc                                     <  2:280302/249‑1 (MQ=255)                                                                                                                                                                                                                     aCTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATc                                                                                                                                                                                 <  2:22142/103‑1 (MQ=255)                                                                                                                                                                                                                     aCTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATc                                                                                                                                                                                 >  1:22142/1‑103 (MQ=255)                                                                                                                                                                                                                       tCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCCTCTTCCGCGGCCCCCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGACAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCAACGTTTATGGACCGCGTGAAGGCCATAAAGgc                             >  2:334961/1‑249 (MQ=255)                                                                                                                                                                                                                           aagAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCAACGTTTATGGACCGCGTGAAGGCCATAAAGGCAGCAtt                       <  2:14095/249‑2 (MQ=255)                                                                                                                                                                                                                           aagAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCAACGTTTATGGACCGCGTGAAGGCCATAAAGGCAGCATg                       >  2:313833/3‑251 (MQ=255)                                                                                                                                                                                                                              aGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTcc                                                                      <  1:222783/201‑1 (MQ=255)                                                                                                                                                                                                                                  gctgcACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCAACGTTTATGGACCGCGTGAAGGCCATAAAGGCAGCATGGCGAGCg                >  2:166901/1‑251 (MQ=255)                                                                                                                                                                                                                                          actGTCTGGAGCGCGAAATAACGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGCGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGCCAAATCCTGCCTGAAGCGCAATCGCAGATTGTTGGCTTCCGTTCTTTACACGTATATGGACAGCGTGAAGGCCATAAAGGCAGCATGGCGTGCGGCGCTTTc        >  2:93519/1‑251 (MQ=255)                                                                                                                                                                                                                                                 ggAGCGCGAAATCCCGTTCCTGGATGCATCTTCAGCGGACACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGGTACTCAAACTTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTTGCAGATTGTTGGCTTCCGTTATTTCAACGTTTATGGACCGCGTGAAGGCCATAAAGGCAGCATTGCGAGCGTCGCTTTCCAtctc  >  2:296847/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    CGATATTCTGGTGGTGGACAACCTGAAAGACGGCACCAAGTTTGTGAACCTGGTGGATCTGAATATCGCAGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGTGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCAACGTTTATGGACCGCGTGAAGGCCATAAAGGCAGCATGGCGAGCGTCGCTTTCCATCTC  >  NC_000913/3794061‑3794550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑