Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,794,298	C→T	C104C (TGC→TGT)	rfaD →	ADP‑L‑glycero‑D‑mannoheptose 6‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,794,298	0	C	T	100.0%	188.4 / NA	57	C104C (TGC→TGT)	rfaD	ADP‑L‑glycero‑D‑mannoheptose 6‑epimerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/29);  total (28/29)

GCATCACCGATATTCTGGTGGTGGACAACCTGAAAGACGGCACCAAGTTTGTGAACCTGGTGGATCTGAATATCGCAGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGTGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCAACGTTTATGGACCGCGTGAAGGCCATAAAGGCAGCATGGCGAGCGTCGCTTTC  >  NC_000913/3794054‑3794544                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       gCATCACCGATATTCTGGTGGTGGACAACCTGAAAGACGGCACCAAGTTTGTGAACCTGGTGGATCTGGATATCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAg                                                                                                                                                                                                                                                  <  1:206229/251‑1 (MQ=255)    tCACCGATATTCTGGTGGTGGACAACCTGAAAGACGGCACCAAGTTTGTGAACCTGGTGGATCTGGATATCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCACGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCgc                                                                                                                                                                                                                                               >  1:234911/1‑250 (MQ=255)        cGATATTCTGGTGGTGGACAACCTGAAAGACGGCACCAAGTTTGTGAACCTGGTGGATCTGGATATCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGaa                                                                                                                                                                                                                                            >  2:209972/1‑250 (MQ=255)         gATATTCTGGTGGTGGACAACCTGAAAGACGGCAACAAGTTTGTGAACCTGGTGGATCTGGATATCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAAt                                                                                                                                                                                                                                          <  2:436916/251‑1 (MQ=255)                       ggACAACCACAAAGCCAGCACCACCTTTGTGACCCTGGTGGTTCTCGATCTCGCGGACTATATCGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATTTCGAAGCGATTTTCCACGAAGCCGCATGCTCTTCCACCACCGAGAGGGATGTAAAGTATATGATGGGAAACACCTATCAATACTCCAAACACCTGCTGCAATACTGTCTGGAGCACGAAATCCCATTCCTGTa                                                                                                                                                                                                                              >  2:146211/1‑249 (MQ=255)                                    gACGGCACCAAGTTTGTGAACCTGGTGGATCTGGATATCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGcggc                                                                                                                                                                                                               <  1:171138/251‑4 (MQ=255)                                       ggCACCAAGTTTGTGAACCTGGTGGATCTGGATATCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCAc                                                                                                                                                                                                            >  2:330023/1‑251 (MQ=255)                                         cACCAAGTTTGTGAACCTGGTGGATCTGGATATCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTGCCACGAAGGCGCATGCTCTTCCACCAGCGAGGGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCGGTTCCTGTATGCAGCGTCCGCGGCCACCt                                                                                                                                                                                                          <  1:146211/251‑1 (MQ=255)                                             aaGTTTGTGAACCTGGTGGATATGGATATCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCAACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGAGCGAAATCCCGTTCATGTAGGCATCTTACGCGGCCACCTAcgg                                                                                                                                                                                                      >  1:165330/1‑251 (MQ=255)                                                  tgtgAACCTGGTGGATCTGGATATCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGAc                                                                                                                                                                                                 >  1:256254/1‑251 (MQ=255)                                                        ccTGGTGGATCTGGATATCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCAccc                                                                                                                                                                                           >  1:71251/1‑250 (MQ=255)                                                           ggtggATCTGGATATCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCg                                                                                                                                                                                        >  1:108466/1‑251 (MQ=255)                                                                    ggatatCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTAt                                                                                                                                                                                 <  2:103130/247‑1 (MQ=255)                                                                    ggatatCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGGCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCGCCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTAGCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAACCCCGTTCCTGGATGCATCTTACGCGGCCACCTACGGCGGACGCATCTCCGCCtt                                                                                                                                                                                    <  2:385571/244‑1 (MQ=255)                                                                    ggatatCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGAAGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATTGCAAGTATATGATGGATAACAACTATCATTACTCCAAAGAGCTATTGCACTACTGTCTGGAGCGCGAAATACCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCAGACGCACCTCCGACTTTATTg                                                                                                                                                                               >  1:81556/3‑251 (MQ=255)                                                                       tatCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGCCGCACCCCCGGCttt                                                                                                                                                                                   >  2:207623/1‑244 (MQ=255)                                                                       tatCGCGGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACttt                                                                                                                                                                                   <  1:207623/244‑1 (MQ=255)                                                                                  aaatGGATAAGGAAGACGTCCTGATCCAGATTAGGGCTGGCGAAGAGTGCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTACACGACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGt                                                                                                                                                                   <  2:81556/247‑1 (MQ=255)                                                                                             gAAGATTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAACGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGACAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTACTGCACTACTGTCTGGAGCGCGAACTCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCCACTTTATTGAATTCCGCGAGTAAGAAAAACCGtt                                                                                                                                                      >  1:51677/1‑251 (MQ=255)                                                                                                       tGATCCAGATTATGGCTGGCGAAGAGTTCGGCGCTGTCGAAGCGCTTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTTTTTAATCCCGCGGTTACGAACAACCGTTGAACGTCTAc                                                                                                                                            >  2:138722/1‑251 (MQ=255)                                                                                                        gATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACg                                                                                                                                           <  2:384328/251‑1 (MQ=255)                                                                                                              gATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGGGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGAATTTTTTTAATCCCGCGGGGACGCAAAAACGTTTAACGTCTACGGTTACt                                                                                                                                     >  2:239518/1‑251 (MQ=255)                                                                                                                 tATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGCCTTTATTGAATCCCGCGCGTACGAAAAACCGTTTAACGTCTACGGTTACTCa                                                                                                                                   >  2:251807/1‑250 (MQ=255)                                                                                                                  aTGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCaaa                                                                                                                                 <  1:301444/251‑1 (MQ=255)                                                                                                                     gctggcGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATACCAACTATAAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGTATCCCGCGAGGACGAAAAACCGATGAACGTATACGGGTACTCAAAAtt                                                                                                                              >  1:282498/1‑251 (MQ=255)                                                                                                                       tggcGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTgg                                                                                                                                                                                                                                                    <  1:425097/131‑1 (MQ=255)                                                                                                                       tggcGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTgg                                                                                                                                                                                                                                                    >  2:425097/1‑131 (MQ=255)                                                                                                                         gcGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTg                                                                                                                          <  1:435384/251‑1 (MQ=255)                                                                                                                         gcGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTg                                                                                                                          <  2:238543/251‑1 (MQ=255)                                                                                                                                            cGAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGt                                                                                                        <  1:251807/250‑1 (MQ=255)                                                                                                                                             gAAGCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATCCTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGCCTTTATTTAATCCCGCGAGTACGAAAAACCGGTGAACGTCCCCGGTTACTCAAAATTCCTGTTTGCTGAATATGTTCGTc                                                                                                       >  2:385/1‑250 (MQ=255)                                                                                                                                                gCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAAt                                                                                                   >  2:144672/1‑251 (MQ=255)                                                                                                                                                gCGATTTTCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAAt                                                                                                   <  1:289460/251‑1 (MQ=255)                                                                                                                                                     tttCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGCTGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGCTGAATATGTTCGTCAAATcctgc                                                                                              >  2:63012/1‑251 (MQ=255)                                                                                                                                                     tttCCACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATcctg                                                                                               <  2:14012/250‑1 (MQ=255)                                                                                                                                                        ccACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTTGAGCGCGAAATCCCGTTCCTGGATGCATCTTCCGCGGTCACCTACGGCGGACGCCCCTCCGACTTTAGTGAATCCCGCGAGTACGAAAAACCGTTGAGCGTCTTCGGGTACGCAAAAGTCCTGTTTCATGAATATGTTCGTCAAATcctgcct                                                                                            <  2:189585/250‑1 (MQ=255)                                                                                                                                                        ccACGAAGGCGCATGCTCTTCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTGTGATGAATATGTTCGTCAAAGcctgcctg                                                                                           <  2:306845/251‑1 (MQ=255)                                                                                                                                                                           tCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAAGCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATGCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACGCGCAGAttg                                                                         <  2:220194/250‑1 (MQ=255)                                                                                                                                                                           tCCACCACCGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTGATTGAATCCCGCTAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAAGGCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGAt                                                                           <  1:34762/248‑1 (MQ=255)                                                                                                                                                                               ccaccGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGc                                                                    >  1:253481/1‑251 (MQ=255)                                                                                                                                                                                   cGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGCAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGCCGCACCTCCGACTTTCTTGAATCCCGCGCGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGGCAAATCCTGCCTGAAGAGCACTCGCAGATTGTTCGCTTcc                                                                >  2:353709/1‑251 (MQ=255)                                                                                                                                                                                   cGAGTGGGATGGCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTcc                                                                <  1:315143/251‑1 (MQ=255)                                                                                                                                                                                             ggCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCa                                                       >  2:14614/1‑250 (MQ=255)                                                                                                                                                                                             ggCAAGTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCa                                                       >  1:380609/1‑250 (MQ=255)                                                                                                                                                                                                  gTATATGATGGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCAACGttt                                                 <  1:394800/251‑1 (MQ=255)                                                                                                                                                                                                    atctgatgGATAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAAGCCCGTTCCTGTATGCATCTTCCGCGGCCTCCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGATCGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCAACGTTTa                                                <  2:245934/247‑1 (MQ=255)                                                                                                                                                                                                             aTAACAACTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTTAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCAACTTTTATGGACCGCGt                                      <  1:59596/251‑1 (MQ=255)                                                                                                                                                                                                                   acTATCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCAACGTTTATGGACCGCGTGAAGGc                                <  1:209972/251‑1 (MQ=255)                                                                                                                                                                                                                      aTCAATACTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAAGCCTGCCTGAAGCGAACTCGCAGAGTGTGGGCTGCCGGTAGTTCAACGTTTATGGACCGCGTGAAGGcc                               <  2:280302/249‑1 (MQ=255)                                                                                                                                                                                                                            aCTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATc                                                                                                                                                                           <  2:22142/103‑1 (MQ=255)                                                                                                                                                                                                                            aCTCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATc                                                                                                                                                                           >  1:22142/1‑103 (MQ=255)                                                                                                                                                                                                                              tCCAAAGAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCCTCTTCCGCGGCCCCCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGACAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCAACGTTTATGGACCGCGTGAAGGCCATAAAGgc                       >  2:334961/1‑249 (MQ=255)                                                                                                                                                                                                                                  aagAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCAACGTTTATGGACCGCGTGAAGGCCATAAAGGCAGCAtt                 <  2:14095/249‑2 (MQ=255)                                                                                                                                                                                                                                  aagAGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCAACGTTTATGGACCGCGTGAAGGCCATAAAGGCAGCATg                 >  2:313833/3‑251 (MQ=255)                                                                                                                                                                                                                                     aGCTGCTGCACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTcc                                                                <  1:222783/201‑1 (MQ=255)                                                                                                                                                                                                                                         gctgcACTACTGTCTGGAGCGCGAAATCCCGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCAACGTTTATGGACCGCGTGAAGGCCATAAAGGCAGCATGGCGAGCg          >  2:166901/1‑251 (MQ=255)                                                                                                                                                                                                                                                 actGTCTGGAGCGCGAAATAACGTTCCTGTATGCATCTTCCGCGGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGCGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGCCAAATCCTGCCTGAAGCGCAATCGCAGATTGTTGGCTTCCGTTCTTTACACGTATATGGACAGCGTGAAGGCCATAAAGGCAGCATGGCGTGCGGCGCTTTc  >  2:93519/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       GCATCACCGATATTCTGGTGGTGGACAACCTGAAAGACGGCACCAAGTTTGTGAACCTGGTGGATCTGAATATCGCAGACTATATGGATAAGGAAGACTTCCTGATCCAGATTATGGCTGGCGAAGAGTTCGGCGATGTCGAAGCGATTTTCCACGAAGGCGCGTGCTCTTCCACCACCGAGTGGGACGGCAAGTATATGATGGATAACAACTATCAATACTCCAAGGAGCTGCTGCACTACTGCCTGGAGCGTGAAATCCCGTTCCTGTACGCTTCTTCCGCAGCCACCTACGGCGGACGCACCTCCGACTTTATTGAATCCCGCGAGTACGAAAAACCGTTGAACGTCTACGGTTACTCAAAATTCCTGTTTGATGAATATGTTCGTCAAATCCTGCCTGAAGCGAACTCGCAGATTGTTGGCTTCCGTTATTTCAACGTTTATGGACCGCGTGAAGGCCATAAAGGCAGCATGGCGAGCGTCGCTTTC  >  NC_000913/3794054‑3794544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑