Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,792,402	G→A	N50N (AAC→AAT)	kbl ←	2‑amino‑3‑ketobutyrate CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,792,402	0	G	A	100.0%	191.0 / NA	56	N50N (AAC→AAT)	kbl	2‑amino‑3‑ketobutyrate CoA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/33);  total (23/33)

GTCGGAGATAATGGCGTCTTCCGCACCCAGAAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGCCCGAGATAGTTGTTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGG  >  NC_000913/3792156‑3792644                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   gTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTgg                                                                                                                                                                                                                                                >  2:202072/1‑251 (MQ=255) gTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTAGGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTgg                                                                                                                                                                                                                                                <  1:360889/251‑1 (MQ=255)       gaTAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACaaa                                                                                                                                                                                                                                          <  1:97617/251‑1 (MQ=255)          aaTGTCGTCTTCCGCGCCCAGCAGCGTTTCAAACAGTCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGt                                                                                                                                                                                                                                       <  1:405508/251‑1 (MQ=255)               cGTCTTCCGCGCCCAGCAGCGTTTCAAACAGGCCACCGTTAGCATAAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATg                                                                                                                                                                                                                                  <  2:342570/251‑1 (MQ=255)                    tCCGCGCCCAGCAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGAc                                                                                                                                                                                                                                <  1:177926/248‑1 (MQ=255)                           ccAGCAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCa                                                                                                                                                                                                                      <  1:60634/251‑1 (MQ=255)                                aGCGGTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCTTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGc                                                                                                                                                                                                                 <  1:254232/251‑1 (MQ=255)                                  cGTTACAAACAGGCCCCCTTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTGATGGCTGTCCGGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCGTCCATCAGCca                                                                                                                                                                                                               <  2:219674/251‑1 (MQ=255)                                    tttCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCcac                                                                                                                                                                                                              >  1:312104/1‑250 (MQ=255)                                        aaaCAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAggg                                                                                                                                                                                                          >  2:99722/1‑248 (MQ=255)                                            aGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGGGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGAc                                                                                                                                                                                                                                <  1:47655/224‑1 (MQ=255)                                            aGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGGGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGAc                                                                                                                                                                                                                                >  2:47655/1‑224 (MQ=255)                                            aGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCAGGCCGAAACCGTGAGAATCCATTCCCGCCTGTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATc                                                                                                                                                                                                     <  1:288037/251‑1 (MQ=255)                                                  ccGTAAGCAACAAAGCAGGAAGATTAGAGAATCGAATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCGGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATCCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCtgcttg                                                                                                                                                                                               <  2:325124/251‑1 (MQ=255)                                                                gCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCATGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCGGAGTGCCGCAAAAAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGtaa                                                                                                                                                                                 <  1:402103/251‑1 (MQ=255)                                                                      agagTAGAGCATCGCATCTTCCGTCCCCAGGAATGCCGACAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGCAGCCATGCCGAAACCGTGAGAATCCATTCACGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCACGAGCTAGTTATTGGCACAAAAGTTAATGACGTGTCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCCGACGTAATAATgg                                                                                                                                                                           >  2:345626/1‑250 (MQ=255)                                                                       gagTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATgcg                                                                                                                                                                          >  1:147339/1‑251 (MQ=255)                                                                       gagTAGAGAATCGCATCTCCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTGATGGCTGTCCTGAGTGCCGCAAATAAAACGCCCCGAAGCCATGCCGAAACCGTGAGAATCCATGCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATgcg                                                                                                                                                                          <  1:211146/251‑1 (MQ=255)                                                                           agagAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCCGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGctct                                                                                                                                                                      >  2:30208/1‑251 (MQ=255)                                                                             agaATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCACTCAGATCCGGTTGATTCCCCAGCCCGATATCGTTATTGGCACAACAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGAtcttt                                                                                                                                                                    >  2:189010/1‑250 (MQ=255)                                                                                  cGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGtaata                                                                                                                                                                               >  1:205617/1‑235 (MQ=255)                                                                                  cGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCGTGCTGCGCAGACGtaata                                                                                                                                                                               <  2:205617/235‑1 (MQ=255)                                                                                              ccccAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCg                                                                                                                                                   >  1:66480/1‑251 (MQ=255)                                                                                               cccAGGAATGCCGCCAGGTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGCAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGCTCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCCGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGc                                                                                                                                                  >  1:305313/1‑251 (MQ=255)                                                                                                   gaaatgccgccAGTTTTTGTGCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGGGAGAATCCATCCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGt                                                                                                                                              <  2:326545/246‑1 (MQ=255)                                                                                                      atgccgacAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAGTAACCCGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGCTCCGGATGATTCGCCAGCCCGAGCTAGTTATTGGCACAAGAGTTAATGACGTGTCTTCCATCACCCACAGTGCTCTCTTCTTTCTGCGCAGACGTCATAATACTCTCTTCTTTCAACAACCCTTCCGCGCGTGCg                                                                                                                                           >  2:175999/3‑251 (MQ=255)                                                                                                                              ctTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATCGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAActgc                                                                                                                   >  2:355643/1‑251 (MQ=255)                                                                                                                                ttATGGCTGTCCGGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAACCCGGGAGAACCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAActgc                                                                                                                   <  2:221986/249‑1 (MQ=255)                                                                                                                                             gAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTcc                                                                                                     <  2:340186/250‑1 (MQ=255)                                                                                                                                                           aaaCGCACCGAAGCCATGCCGAACCCGTGAGAATCCATTCCCGCCTTTGCCGCCGAAATCAGATCCGGATGATTCGCCCGCCCGAGATAGTTATTGGCCCAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGCCGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTACCTGCTGATCAAATTCTCCACGCATTGCGATtct                                                                                      >  2:56514/1‑251 (MQ=255)                                                                                                                                                           aaaCGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATtct                                                                                      <  2:66480/251‑1 (MQ=255)                                                                                                                                                                    gAAGCCATGCCGAAACCGTGAGAATCCAGTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCAGCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAgg                                                                             <  1:112605/251‑1 (MQ=255)                                                                                                                                                                     aaGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAggg                                                                            <  1:233616/251‑1 (MQ=255)                                                                                                                                                                        ccATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCtgcttgc                                                                                                                                                                                              >  1:432411/1‑134 (MQ=255)                                                                                                                                                                        ccATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCtgcttgc                                                                                                                                                                                              <  2:432411/134‑1 (MQ=255)                                                                                                                                                                                    cGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACat                                                             >  1:30643/1‑251 (MQ=255)                                                                                                                                                                                       gagaATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATAtt                                                          <  2:147339/251‑1 (MQ=255)                                                                                                                                                                                              cATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCaaa                                                   <  1:153333/251‑1 (MQ=255)                                                                                                                                                                                               aTTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAAc                                                  <  2:353830/251‑1 (MQ=255)                                                                                                                                                                                               aTTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAAc                                                  >  1:273210/1‑251 (MQ=255)                                                                                                                                                                                                 tCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACt                                                 <  1:132554/250‑1 (MQ=255)                                                                                                                                                                                                   ccGCCTTTGCCGCCGCAATCAGATCCGTATGATTCTCCAGCCCGAGATAGTTATTAGCACAAAAGTTAATGACGTGGCTTCCTTCAGCCACAGTGATATCTGCTTGCTTCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCATCACATATTACCCAAACTtat                                              <  1:1360/251‑1 (MQ=255)                                                                                                                                                                                                     gCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTtat                                              >  1:331148/1‑249 (MQ=255)                                                                                                                                                                                                         ttGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCGGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGt                                          <  1:216719/249‑1 (MQ=255)                                                                                                                                                                                                             cgccgcAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGcgac                                    <  1:5350/251‑1 (MQ=255)                                                                                                                                                                                                              gccgcAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGcgacg                                   >  1:385317/1‑251 (MQ=255)                                                                                                                                                                                                                   aaTCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATaaaa                                                                                                           >  2:224147/1‑174 (MQ=255)                                                                                                                                                                                                                   aaTCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATaaaa                                                                                                           <  1:224147/174‑1 (MQ=255)                                                                                                                                                                                                                     tcagatccgGATGCTTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATAACTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATATGTCGCGACGAGAtgac                            >  1:370578/1‑248 (MQ=255)                                                                                                                                                                                                                      cagatccgGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGGTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGAtgac                            <  2:273210/250‑4 (MQ=255)                                                                                                                                                                                                                           ccggATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCTTCGAGATGACGCGTTa                      <  2:425320/249‑1 (MQ=255)                                                                                                                                                                                                                             ggATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGttt                                                                                                                                       >  2:345329/1‑136 (MQ=255)                                                                                                                                                                                                                             ggATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGttt                                                                                                                                       <  1:345329/136‑1 (MQ=255)                                                                                                                                                                                                                             ggATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATGACGCGTTAac                    <  1:311330/251‑1 (MQ=255)                                                                                                                                                                                                                                                gaTAGTTATTGGCAAAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATGACGCGTTAACACTTCTGCAAAATTCAgg  >  1:306845/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   GTCGGAGATAATGGCGTCTTCCGCACCCAGAAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGCCCGAGATAGTTGTTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAACGCGTTAACACTTCTGCAAAATTCAGG  >  NC_000913/3792156‑3792644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑