Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,792,375	A→C	P59P (CCT→CCG)	kbl ←	2‑amino‑3‑ketobutyrate CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,792,375	0	A	C	100.0%	197.9 / NA	56	P59P (CCT→CCG)	kbl	2‑amino‑3‑ketobutyrate CoA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (23/33);  total (23/33)

ATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCACCCAGAAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGCCCGAGATAGTTGTTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAAC  >  NC_000913/3792129‑3792618                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    aTCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGgat                                                                                                                                                                                                                                                 <  1:257775/251‑1 (MQ=255)        ataGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGc                                                                                                                                                                                                                                          >  2:360889/1‑251 (MQ=255)         aaGACGCGTGGTTCAGTGCGGCGGAGAAAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAACCAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAACCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGcc                                                                                                                                                                                                                                         <  2:129520/250‑1 (MQ=255)                    ttCAGTGCGTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGAt                                                                                                                                                                                                                               >  2:177926/1‑250 (MQ=255)                      cAGTGCGTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAgt                                                                                                                                                                                                                            >  2:5350/1‑251 (MQ=255)                        gTGCGTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAACAGGCCCCCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAgtta                                                                                                                                                                                                                          <  2:2130/251‑2 (MQ=255)                           cGTCGGAGAGAATTGCGTCTACCGCTCCCCGCAGCGTTTCAAACAGGCCACCGTTAGCACAAAAGCAGGAGGATTAGAGAATCCCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTTTCCTGATTCCCGCAATTAAAACGCACCGAAGCCATGCCGAAACCGTGATAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCTGCCCGAGATAgttattt                                                                                                                                                                                                                       <  2:368025/251‑5 (MQ=255)                            gTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTgg                                                                                                                                                                                                                      >  2:202072/1‑251 (MQ=255)                            gTCGGAGATAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTAGGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTgg                                                                                                                                                                                                                      <  1:360889/251‑1 (MQ=255)                                  gaTAATGGCGTCTTCCGCGCCCAGCAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACaaa                                                                                                                                                                                                                <  1:97617/251‑1 (MQ=255)                                     aaTGTCGTCTTCCGCGCCCAGCAGCGTTTCAAACAGTCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGt                                                                                                                                                                                                             <  1:405508/251‑1 (MQ=255)                                          cGTCTTCCGCGCCCAGCAGCGTTTCAAACAGGCCACCGTTAGCATAAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATg                                                                                                                                                                                                        <  2:342570/251‑1 (MQ=255)                                               tCCGCGCCCAGCAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGAc                                                                                                                                                                                                      <  1:177926/248‑1 (MQ=255)                                                      ccAGCAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCa                                                                                                                                                                                            <  1:60634/251‑1 (MQ=255)                                                           aGCGGTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCTTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGc                                                                                                                                                                                       <  1:254232/251‑1 (MQ=255)                                                             cGTTACAAACAGGCCCCCTTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTGATGGCTGTCCGGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCGTCCATCAGCca                                                                                                                                                                                     <  2:219674/251‑1 (MQ=255)                                                               tttCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCcac                                                                                                                                                                                    >  1:312104/1‑250 (MQ=255)                                                                   aaaCAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAggg                                                                                                                                                                                >  2:99722/1‑248 (MQ=255)                                                                       aGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGGGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGAc                                                                                                                                                                                                      >  2:47655/1‑224 (MQ=255)                                                                       aGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGGGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGAc                                                                                                                                                                                                      <  1:47655/224‑1 (MQ=255)                                                                       aGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCAGGCCGAAACCGTGAGAATCCATTCCCGCCTGTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATc                                                                                                                                                                           <  1:288037/251‑1 (MQ=255)                                                                             ccGTAAGCAACAAAGCAGGAAGATTAGAGAATCGAATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCGGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATCCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCtgcttg                                                                                                                                                                     <  2:325124/251‑1 (MQ=255)                                                                                           gCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCATGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCGGAGTGCCGCAAAAAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGtaa                                                                                                                                                       <  1:402103/251‑1 (MQ=255)                                                                                                 agagTAGAGCATCGCATCTTCCGTCCCCAGGAATGCCGACAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGCAGCCATGCCGAAACCGTGAGAATCCATTCACGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCACGAGCTAGTTATTGGCACAAAAGTTAATGACGTGTCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCCGACGTAATAATgg                                                                                                                                                 >  2:345626/1‑250 (MQ=255)                                                                                                  gagTAGAGAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATgcg                                                                                                                                                >  1:147339/1‑251 (MQ=255)                                                                                                  gagTAGAGAATCGCATCTCCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTGATGGCTGTCCTGAGTGCCGCAAATAAAACGCCCCGAAGCCATGCCGAAACCGTGAGAATCCATGCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATgcg                                                                                                                                                <  1:211146/251‑1 (MQ=255)                                                                                                      agagAATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCCGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGctct                                                                                                                                            >  2:30208/1‑251 (MQ=255)                                                                                                        agaATCGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCACTCAGATCCGGTTGATTCCCCAGCCCGATATCGTTATTGGCACAACAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGAtcttt                                                                                                                                          >  2:189010/1‑250 (MQ=255)                                                                                                             cGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGtaata                                                                                                                                                     >  1:205617/1‑235 (MQ=255)                                                                                                             cGCATCTTCCATCCCCAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCGTGCTGCGCAGACGtaata                                                                                                                                                     <  2:205617/235‑1 (MQ=255)                                                                                                                         ccccAGGAATGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCg                                                                                                                         >  1:66480/1‑251 (MQ=255)                                                                                                                          cccAGGAATGCCGCCAGGTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGCAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGCTCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCCGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGc                                                                                                                        >  1:305313/1‑251 (MQ=255)                                                                                                                              gaaatgccgccAGTTTTTGTGCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGGGAGAATCCATCCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGt                                                                                                                    <  2:326545/246‑1 (MQ=255)                                                                                                                                 atgccgacAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAGTAACCCGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGCTCCGGATGATTCGCCAGCCCGAGCTAGTTATTGGCACAAGAGTTAATGACGTGTCTTCCATCACCCACAGTGCTCTCTTCTTTCTGCGCAGACGTCATAATACTCTCTTCTTTCAACAACCCTTCCGCGCGTGCg                                                                                                                 >  2:175999/3‑251 (MQ=255)                                                                                                                                                         ctTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATCGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAActgc                                                                                         >  2:355643/1‑251 (MQ=255)                                                                                                                                                           ttATGGCTGTCCGGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAACCCGGGAGAACCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAActgc                                                                                         <  2:221986/249‑1 (MQ=255)                                                                                                                                                                        gAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTcc                                                                           <  2:340186/250‑1 (MQ=255)                                                                                                                                                                                      aaaCGCACCGAAGCCATGCCGAACCCGTGAGAATCCATTCCCGCCTTTGCCGCCGAAATCAGATCCGGATGATTCGCCCGCCCGAGATAGTTATTGGCCCAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGCCGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTACCTGCTGATCAAATTCTCCACGCATTGCGATtct                                                            >  2:56514/1‑251 (MQ=255)                                                                                                                                                                                      aaaCGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATtct                                                            <  2:66480/251‑1 (MQ=255)                                                                                                                                                                                               gAAGCCATGCCGAAACCGTGAGAATCCAGTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCAGCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAgg                                                   <  1:112605/251‑1 (MQ=255)                                                                                                                                                                                                aaGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAggg                                                  <  1:233616/251‑1 (MQ=255)                                                                                                                                                                                                   ccATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCtgcttgc                                                                                                                                                                    >  1:432411/1‑134 (MQ=255)                                                                                                                                                                                                   ccATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCtgcttgc                                                                                                                                                                    <  2:432411/134‑1 (MQ=255)                                                                                                                                                                                                               cGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACat                                   >  1:30643/1‑251 (MQ=255)                                                                                                                                                                                                                  gagaATCCATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATAtt                                <  2:147339/251‑1 (MQ=255)                                                                                                                                                                                                                         cATTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCaaa                         <  1:153333/251‑1 (MQ=255)                                                                                                                                                                                                                          aTTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAAc                        >  1:273210/1‑251 (MQ=255)                                                                                                                                                                                                                          aTTCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAAc                        <  2:353830/251‑1 (MQ=255)                                                                                                                                                                                                                            tCCCGCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACt                       <  1:132554/250‑1 (MQ=255)                                                                                                                                                                                                                              ccGCCTTTGCCGCCGCAATCAGATCCGTATGATTCTCCAGCCCGAGATAGTTATTAGCACAAAAGTTAATGACGTGGCTTCCTTCAGCCACAGTGATATCTGCTTGCTTCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCATCACATATTACCCAAACTtat                    <  1:1360/251‑1 (MQ=255)                                                                                                                                                                                                                                gCCTTTGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTtat                    >  1:331148/1‑249 (MQ=255)                                                                                                                                                                                                                                    ttGCCGCCGCAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCGGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGt                <  1:216719/249‑1 (MQ=255)                                                                                                                                                                                                                                        cgccgcAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGcgac          <  1:5350/251‑1 (MQ=255)                                                                                                                                                                                                                                         gccgcAATCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGcgacg         >  1:385317/1‑251 (MQ=255)                                                                                                                                                                                                                                              aaTCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATaaaa                                                                                 >  2:224147/1‑174 (MQ=255)                                                                                                                                                                                                                                              aaTCAGATCCGGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATaaaa                                                                                 <  1:224147/174‑1 (MQ=255)                                                                                                                                                                                                                                                tcagatccgGATGCTTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATAACTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATATGTCGCGACGAGAtgac  >  1:370578/1‑248 (MQ=255)                                                                                                                                                                                                                                                 cagatccgGATGATTCGCCAGCCCGAGATAGTTATTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCGCGTGCGGTTTCCAGATCGGTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGAtgac  <  2:273210/250‑4 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    ATCAATAATAGACGCGTGGTTCAGTGCGTCGGAGATAATGGCGTCTTCCGCACCCAGAAGCGTTTCAAACAGGCCACCGTTAGCATCAAAGCAGGAAGAGTAGAGAATCGCATCTTCCATCCCCAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGCCCGAGATAGTTGTTGGCACAAAAGTTAATGACGTGGCTTCCATCAGCCACAGTGATATCTGCTTGCTGCGCAGACGTAATAATGCGCTCTTCTTTAAACAACCCTTCCGCCCGTGCGGTTTCCAGATCGTTGGTTAACTGCTGATAAAATTCTCCACGCATTGCGATTCTCCAGACAGGGCAAATTCCAGCACATATTACCCAAACTTATAGGTCGCGACGAGATAAC  >  NC_000913/3792129‑3792618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑