Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,791,694	G→A	I286I (ATC→ATT)	kbl ←	2‑amino‑3‑ketobutyrate CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,791,694	0	G	A	100.0%	203.6 / NA	59	I286I (ATC→ATT)	kbl	2‑amino‑3‑ketobutyrate CoA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/21);  total (38/21)

GAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTAACGTAAATGCCCTCTTTTTGCAGCTCACGGGCAAATTTCTGCGCCACTACCGCATCACCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGTTCGCTGCCCGCTTCGACCATCTCCAGTACTTTGATGGACGCGGCAACAATGGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGCGTACCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCATTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGC  >  NC_000913/3791450‑3791939                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      gAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGa                                                                                                                                                                                                                                                  >  1:184645/1‑250 (MQ=255) gAATACGCGCCTGACCTCTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACAGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGATGCCCGCTTCAACCCTCTCCAGCACTTTAATGGAc                                                                                                                                                                                                                                                 >  2:224452/1‑251 (MQ=255)   aTACGCGCCTGACCTTTCGGAACGCCCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTTCAGCTCACTATCAAATTTCTGCGCCACTACCGCATCGCCAATCATGACCGGAATAATTGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGaagc                                                                                                                                                                                                                                               <  1:280897/251‑4 (MQ=255)       gcgcCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGcg                                                                                                                                                                                                                                           <  2:355596/251‑2 (MQ=255)               acTTTCGGAACGACCGGATAAAAGAAACCGGTGACGCAAATGCCCTCTTTTTGCAACTCACGAGCAAATTTCTGCGCCACTACCGCCTCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGATCGCTGCCCGCTTCAACCATCTCCAGCCCTTTAATGGAAACGGcaacaaaagc                                                                                                                                                                                                                                   >  2:166194/2‑247 (MQ=255)                cTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGcc                                                                                                                                                                                                                                  >  1:305663/1‑251 (MQ=255)                       aaCGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGcc                                                                                                                                                                                                                            >  1:160718/1‑250 (MQ=255)                                  agaagaAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCCCCCCTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGACCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGga                                                                                                                                                                                                                 <  2:424432/250‑1 (MQ=255)                                  agaagaAACCGGTGACGGAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGgag                                                                                                                                                                                                                <  1:358402/251‑1 (MQ=255)                                   gaagaaACCGTGGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCCCTACCGCACCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGgaga                                                                                                                                                                                                               <  2:305744/251‑1 (MQ=255)                                       aaaCCGGCGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCCCGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGgagcaccg                                                                                                                                                                                                           >  2:186026/1‑246 (MQ=255)                                                 cGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACCGGTACgggcg                                                                                                                                                                                                  >  2:36878/1‑247 (MQ=255)                                                    aaaTGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTAACGCATCGCCAAGCATGACCGGAAGAATGGCGTGATCGGCACCCGCCAGGGAAAAGCACGCCGCCGACATTGGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGGGACAAGAGCCGGTGCAAGCGAGTTGGAGAACAGGGACGGACgaga                                                                                                                                                                                               >  1:143653/1‑250 (MQ=255)                                                          cgtccttttGCAGCTACCGAGCAATTTTCTGCGCCACCACCGCACCTCCAAGCATGACCGGAATAATGGCGCGATCGGCTTCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTACGCCCACAGACGGTCACGCAGCGCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGGGCCAGCTAGTTGGAGAACAGGTACGGACGAGAACGCTgg                                                                                                                                                                                        <  1:14101/246‑1 (MQ=255)                                                                 ttGCAGCTCACGAGCAAATTTCTGCGCCACTCCCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGc                                                                                                                                                                                  <  1:317588/250‑1 (MQ=255)                                                                      gCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCa                                                                                                                                                                            <  1:121210/251‑1 (MQ=255)                                                                            gagCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGCCGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGGGCCCGCGAGTTGGAGAACAGGGACGGACGAGAACGCTGGCGCAGCCCCCCAAcccct                                                                                                                                                                      >  2:101513/3‑248 (MQ=255)                                                                            gagCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCAct                                                                                                                                                                      >  1:183512/3‑251 (MQ=255)                                                                                aaaTTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGAAAAGCCCGCCGCCGATATTTGCTCACGGAACTGACGCGCGTTCGCCCACCGACGGTCACGCAGCTCGCTGCCCGCTTCAAACATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCCGCGCCAGCGGGTTGGAGAACAGGGACGGACGAGAACGCTGGCGCAGCCACTCAACCActtctt                                                                                                                                                                  >  1:10465/1‑251 (MQ=255)                                                                                 aaTTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCttt                                                                                                                                                                 >  1:412243/1‑251 (MQ=255)                                                                                  aTTTCTGCGCCACTACCGCCTCGCCAAGCATGACCGGAATAATGGCGTGCTCGGCTACCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCCGCGAGTTGGAGaa                                                                                                                                                                                                              >  2:25668/1‑205 (MQ=255)                                                                                  aTTTCTGCGCCACGACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGaa                                                                                                                                                                                                              <  1:25668/205‑1 (MQ=255)                                                                                          gcCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGAAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGcgccgc                                                                                                                                                        >  2:368849/1‑251 (MQ=255)                                                                                               tACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGAAGAGCGTTCGCCCACAGAAGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATg                                                                                                                                                                                                                                                    >  1:382997/1‑154 (MQ=255)                                                                                                aCCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGGACGGACGAGAACGCTGGCGCCGCCCCTCAACCAATTCTTTGCGCGCAGCgggat                                                                                                                                                   >  2:367933/1‑247 (MQ=255)                                                                                                 ccGCATCACCAACCATCACCGGAATAATCGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTAACGGAACTGACGAGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGCCAATAGCCGGTGCCAGCGAGTTGGCGGACAGGTACGGACGAGCACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGACGCGGtata                                                                                                                                                  >  2:220785/1‑250 (MQ=255)                                                                                                            aGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGc                                                                                                                                      <  1:387391/251‑1 (MQ=255)                                                                                                                        ataatGGCGTGATCGGCTCCCGCCCGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAgcgc                                                                                                                          >  2:9779/1‑251 (MQ=255)                                                                                                                           atGGCGTGATCGGCTCCCCCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCCACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCttt                                                                                                                       >  1:302804/1‑251 (MQ=255)                                                                                                                              gcgttaccGGCCCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTAcc                                                                                                                    <  2:109202/244‑1 (MQ=255)                                                                                                                              gCGTGACCGTCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCGGCCCGCTTCAACCATCTCCAGCACTTGAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCTCTCAACCACTGCTTTGCGCGCCGCGGTATAACCACCAGAAGCCGCGCCCAGCGCTTTAcc                                                                                                                    <  2:367787/251‑1 (MQ=255)                                                                                                                                   ccggccTCCCGCCAGTGTAAAGCCCGCCGCCGACATTGGCTCCCGGAACTGACGCGCGTTCGCCCACAGGCGGTCACGCAGCTCGCTGCCCGTTGCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCGAGCGCTTTGCCAAgtg                                                                                                               <  2:53349/246‑3 (MQ=255)                                                                                                                                             gCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGACCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGAAGACACTCACCCACTTCATTGCGCGCCGCGTGATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGtatt                                                                                                     >  2:101614/1‑248 (MQ=255)                                                                                                                                               cAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGata                                                                                                    >  1:115485/1‑250 (MQ=255)                                                                                                                                                aGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGataat                                                                                                  >  1:278470/1‑251 (MQ=255)                                                                                                                                                              cgccgcaaTTTGCTCACGGAACTGACGCGCGTTCGCCCAAAGACGGTCACGCCGCTCGCTGCCCGCGTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGCGAACGCTGGCGCAGCAACTCAACCACTTCTTTGCTCGCCGCGGTATAACCACCAGAAGACCCGCCCAGCGCTTTACCAAGTGTGCCGATGATAATATCGACCCAGcccc                                                                                    >  1:368025/8‑250 (MQ=255)                                                                                                                                                              cgccgACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGCGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCACGTGTGCCGGTGATAATATCGACCCGGcccc                                                                                    >  2:426857/1‑250 (MQ=255)                                                                                                                                                               gccgACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCGGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGCGTGCCGGGGATAATATCGACCCGGCCCAt                                                                                   >  2:127555/1‑251 (MQ=255)                                                                                                                                                                ccgACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCTGGTGATAATATCGACCCGGCCCATc                                                                                  >  1:278140/1‑251 (MQ=255)                                                                                                                                                                 cgACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCaac                                                                                  >  2:314888/1‑248 (MQ=255)                                                                                                                                                                 cgACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATc                                                                                  >  2:320691/1‑250 (MQ=255)                                                                                                                                                                   aCATTTGCTCACGGAACTGGCGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCACCCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGCACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTCTAACCACCAGAAGCCCCGCCCAGCGGTTTACCCAGTGTGCCGGTGATAATATCGACCCGGCCCATcac                                                                                >  2:393709/1‑250 (MQ=255)                                                                                                                                                                            ctcGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGt                                                                        <  1:245698/247‑1 (MQ=255)                                                                                                                                                                                    tGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGGGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTAtt                                                                     >  2:267075/1‑244 (MQ=255)                                                                                                                                                                                    tGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCACCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTAtt                                                                     <  1:267075/244‑1 (MQ=255)                                                                                                                                                                                       cccgcgTGCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAAcc                                                           <  2:218575/249‑1 (MQ=255)                                                                                                                                                                                                  ccACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGtttt                                                >  2:42440/1‑251 (MQ=255)                                                                                                                                                                                                               cGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAacc                                   >  2:40573/1‑251 (MQ=255)                                                                                                                                                                                                               cGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAAGGGAAGCGGCGAGAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGGCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAacc                                   <  1:393709/251‑1 (MQ=255)                                                                                                                                                                                                                       gCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGCAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGt                           >  1:206085/1‑251 (MQ=255)                                                                                                                                                                                                                       gCTGCCCGCTTCAACCATCTCCAGCACTTTAAGGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGt                           <  2:183512/251‑1 (MQ=255)                                                                                                                                                                                                                        cTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTg                          >  1:268760/1‑251 (MQ=255)                                                                                                                                                                                                                           cccGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTggg                        >  2:375032/1‑250 (MQ=255)                                                                                                                                                                                                                            ccGCTTCAACCAACTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGCCCGCGTGGGAg                      <  2:433895/251‑1 (MQ=255)                                                                                                                                                                                                                                ttcaaccctctcCAGCACTTTAATGGACGCGGCGCCAATAGCCGGTGCCCGCGCGTTGGAGAACAGGTACGGACGAGACCGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGCATAACCACCAGAAGCCCCCCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACCTCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAgtcg                   >  2:178821/9‑250 (MQ=255)                                                                                                                                                                                                                                ttcaaCCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAgtcgt                  >  1:123030/5‑251 (MQ=255)                                                                                                                                                                                                                                            cagcaCTTTAATGGAAGCGGCGACAAGTGCCGGTGCCAGTGAGTTGGAGAACAGGTACGGACGAGAACGCGGGCTCAGCCACTCAACCACTTCTGTGCGCGCCGCGGTCTAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCTCCa      <  2:334403/247‑1 (MQ=255)                                                                                                                                                                                                                                             agcaCTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAg     <  1:101513/248‑1 (MQ=255)                                                                                                                                                                                                                                                 cTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGc  >  2:312778/1‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      GAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTAACGTAAATGCCCTCTTTTTGCAGCTCACGGGCAAATTTCTGCGCCACTACCGCATCACCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGTTCGCTGCCCGCTTCGACCATCTCCAGTACTTTGATGGACGCGGCAACAATGGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGCGTACCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCATTTTCACCGACAAAACCGACCGCGTGGGAGTCGTCTACCATCACCAGGGC  >  NC_000913/3791450‑3791939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑