Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,791,688	T→C	V288V (GTA→GTG)	kbl ←	2‑amino‑3‑ketobutyrate CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,791,688	0	T	C	100.0%	179.6 / NA	57	V288V (GTA→GTG)	kbl	2‑amino‑3‑ketobutyrate CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/19);  total (38/19)

TGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTAACGTAAATGCCCTCTTTTTGCAGCTCACGGGCAAATTTCTGCGCCACTACCGCATCACCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGTTCGCTGCCCGCTTCGACCATCTCCAGTACTTTGATGGACGCGGCAACAATGGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGCGTACCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCATTTTCACCGACAAAACCGACCGCGTGGGAGTCGT  >  NC_000913/3791443‑3791923                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            tGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACttt                                                                                                                                                                                                                                        >  1:232240/1‑251 (MQ=255)        gAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGa                                                                                                                                                                                                                                  >  1:184645/1‑250 (MQ=255)        gAATACGCGCCTGACCTCTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACAGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGATGCCCGCTTCAACCCTCTCCAGCACTTTAATGGAc                                                                                                                                                                                                                                 >  2:224452/1‑251 (MQ=255)          aTACGCGCCTGACCTTTCGGAACGCCCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTTCAGCTCACTATCAAATTTCTGCGCCACTACCGCATCGCCAATCATGACCGGAATAATTGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGaagc                                                                                                                                                                                                                               <  1:280897/251‑4 (MQ=255)              gcgcCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGcg                                                                                                                                                                                                                           <  2:355596/251‑2 (MQ=255)                      acTTTCGGAACGACCGGATAAAAGAAACCGGTGACGCAAATGCCCTCTTTTTGCAACTCACGAGCAAATTTCTGCGCCACTACCGCCTCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGATCGCTGCCCGCTTCAACCATCTCCAGCCCTTTAATGGAAACGGcaacaaaagc                                                                                                                                                                                                                   >  2:166194/2‑247 (MQ=255)                       cTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGcc                                                                                                                                                                                                                  >  1:305663/1‑251 (MQ=255)                              aaCGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGcc                                                                                                                                                                                                            >  1:160718/1‑250 (MQ=255)                                         agaagaAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCCCCCCTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGACCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGga                                                                                                                                                                                                 <  2:424432/250‑1 (MQ=255)                                         agaagaAACCGGTGACGGAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGgag                                                                                                                                                                                                <  1:358402/251‑1 (MQ=255)                                          gaagaaACCGTGGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCCCTACCGCACCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGgaga                                                                                                                                                                                               <  2:305744/251‑1 (MQ=255)                                              aaaCCGGCGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCCCGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGgagcaccg                                                                                                                                                                                           >  2:186026/1‑246 (MQ=255)                                                        cGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACCGGTACgggcg                                                                                                                                                                                  >  2:36878/1‑247 (MQ=255)                                                           aaaTGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTAACGCATCGCCAAGCATGACCGGAAGAATGGCGTGATCGGCACCCGCCAGGGAAAAGCACGCCGCCGACATTGGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGGGACAAGAGCCGGTGCAAGCGAGTTGGAGAACAGGGACGGACgaga                                                                                                                                                                               >  1:143653/1‑250 (MQ=255)                                                                 cgtccttttGCAGCTACCGAGCAATTTTCTGCGCCACCACCGCACCTCCAAGCATGACCGGAATAATGGCGCGATCGGCTTCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTACGCCCACAGACGGTCACGCAGCGCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGGGCCAGCTAGTTGGAGAACAGGTACGGACGAGAACGCTgg                                                                                                                                                                        <  1:14101/246‑1 (MQ=255)                                                                        ttGCAGCTCACGAGCAAATTTCTGCGCCACTCCCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGc                                                                                                                                                                  <  1:317588/250‑1 (MQ=255)                                                                             gCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCa                                                                                                                                                            <  1:121210/251‑1 (MQ=255)                                                                                   gagCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGCCGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGGGCCCGCGAGTTGGAGAACAGGGACGGACGAGAACGCTGGCGCAGCCCCCCAAcccct                                                                                                                                                      >  2:101513/3‑248 (MQ=255)                                                                                   gagCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCAct                                                                                                                                                      >  1:183512/3‑251 (MQ=255)                                                                                       aaaTTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGAAAAGCCCGCCGCCGATATTTGCTCACGGAACTGACGCGCGTTCGCCCACCGACGGTCACGCAGCTCGCTGCCCGCTTCAAACATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCCGCGCCAGCGGGTTGGAGAACAGGGACGGACGAGAACGCTGGCGCAGCCACTCAACCActtctt                                                                                                                                                  >  1:10465/1‑251 (MQ=255)                                                                                        aaTTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCttt                                                                                                                                                 >  1:412243/1‑251 (MQ=255)                                                                                         aTTTCTGCGCCACTACCGCCTCGCCAAGCATGACCGGAATAATGGCGTGCTCGGCTACCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCCGCGAGTTGGAGaa                                                                                                                                                                                              >  2:25668/1‑205 (MQ=255)                                                                                         aTTTCTGCGCCACGACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGaa                                                                                                                                                                                              <  1:25668/205‑1 (MQ=255)                                                                                                 gcCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGAAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGcgccgc                                                                                                                                        >  2:368849/1‑251 (MQ=255)                                                                                                      tACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGAAGAGCGTTCGCCCACAGAAGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATg                                                                                                                                                                                                                                    >  1:382997/1‑154 (MQ=255)                                                                                                       aCCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGGACGGACGAGAACGCTGGCGCCGCCCCTCAACCAATTCTTTGCGCGCAGCgggat                                                                                                                                   >  2:367933/1‑247 (MQ=255)                                                                                                        ccGCATCACCAACCATCACCGGAATAATCGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTAACGGAACTGACGAGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGCCAATAGCCGGTGCCAGCGAGTTGGCGGACAGGTACGGACGAGCACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGACGCGGtata                                                                                                                                  >  2:220785/1‑250 (MQ=255)                                                                                                                   aGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGc                                                                                                                      <  1:387391/251‑1 (MQ=255)                                                                                                                               ataatGGCGTGATCGGCTCCCGCCCGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAgcgc                                                                                                          >  2:9779/1‑251 (MQ=255)                                                                                                                                  atGGCGTGATCGGCTCCCCCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCCACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCttt                                                                                                       >  1:302804/1‑251 (MQ=255)                                                                                                                                     gcgttaccGGCCCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTAcc                                                                                                    <  2:109202/244‑1 (MQ=255)                                                                                                                                     gCGTGACCGTCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCGGCCCGCTTCAACCATCTCCAGCACTTGAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCTCTCAACCACTGCTTTGCGCGCCGCGGTATAACCACCAGAAGCCGCGCCCAGCGCTTTAcc                                                                                                    <  2:367787/251‑1 (MQ=255)                                                                                                                                          ccggccTCCCGCCAGTGTAAAGCCCGCCGCCGACATTGGCTCCCGGAACTGACGCGCGTTCGCCCACAGGCGGTCACGCAGCTCGCTGCCCGTTGCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCGAGCGCTTTGCCAAgtg                                                                                               <  2:53349/246‑3 (MQ=255)                                                                                                                                                    gCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGACCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGAAGACACTCACCCACTTCATTGCGCGCCGCGTGATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGtatt                                                                                     >  2:101614/1‑248 (MQ=255)                                                                                                                                                      cAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGata                                                                                    >  1:115485/1‑250 (MQ=255)                                                                                                                                                       aGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGataat                                                                                  >  1:278470/1‑251 (MQ=255)                                                                                                                                                                     cgccgcaaTTTGCTCACGGAACTGACGCGCGTTCGCCCAAAGACGGTCACGCCGCTCGCTGCCCGCGTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGCGAACGCTGGCGCAGCAACTCAACCACTTCTTTGCTCGCCGCGGTATAACCACCAGAAGACCCGCCCAGCGCTTTACCAAGTGTGCCGATGATAATATCGACCCAGcccc                                                                    >  1:368025/8‑250 (MQ=255)                                                                                                                                                                     cgccgACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGCGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCACGTGTGCCGGTGATAATATCGACCCGGcccc                                                                    >  2:426857/1‑250 (MQ=255)                                                                                                                                                                      gccgACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCGGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGCGTGCCGGGGATAATATCGACCCGGCCCAt                                                                   >  2:127555/1‑251 (MQ=255)                                                                                                                                                                       ccgACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCTGGTGATAATATCGACCCGGCCCATc                                                                  >  1:278140/1‑251 (MQ=255)                                                                                                                                                                        cgACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCaac                                                                  >  2:314888/1‑248 (MQ=255)                                                                                                                                                                        cgACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATc                                                                  >  2:320691/1‑250 (MQ=255)                                                                                                                                                                          aCATTTGCTCACGGAACTGGCGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCACCCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGCACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTCTAACCACCAGAAGCCCCGCCCAGCGGTTTACCCAGTGTGCCGGTGATAATATCGACCCGGCCCATcac                                                                >  2:393709/1‑250 (MQ=255)                                                                                                                                                                                   ctcGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGt                                                        <  1:245698/247‑1 (MQ=255)                                                                                                                                                                                           tGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGGGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTAtt                                                     >  2:267075/1‑244 (MQ=255)                                                                                                                                                                                           tGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCACCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTAtt                                                     <  1:267075/244‑1 (MQ=255)                                                                                                                                                                                              cccgcgTGCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAAcc                                           <  2:218575/249‑1 (MQ=255)                                                                                                                                                                                                         ccACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGtttt                                >  2:42440/1‑251 (MQ=255)                                                                                                                                                                                                                      cGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAacc                   >  2:40573/1‑251 (MQ=255)                                                                                                                                                                                                                      cGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAAGGGAAGCGGCGAGAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGGCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAacc                   <  1:393709/251‑1 (MQ=255)                                                                                                                                                                                                                              gCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGCAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGt           >  1:206085/1‑251 (MQ=255)                                                                                                                                                                                                                              gCTGCCCGCTTCAACCATCTCCAGCACTTTAAGGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGt           <  2:183512/251‑1 (MQ=255)                                                                                                                                                                                                                               cTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTg          >  1:268760/1‑251 (MQ=255)                                                                                                                                                                                                                                  cccGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTggg        >  2:375032/1‑250 (MQ=255)                                                                                                                                                                                                                                   ccGCTTCAACCAACTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGCCCGCGTGGGAg      <  2:433895/251‑1 (MQ=255)                                                                                                                                                                                                                                       ttcaaccctctcCAGCACTTTAATGGACGCGGCGCCAATAGCCGGTGCCCGCGCGTTGGAGAACAGGTACGGACGAGACCGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGCATAACCACCAGAAGCCCCCCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACCTCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAgtcg   >  2:178821/9‑250 (MQ=255)                                                                                                                                                                                                                                       ttcaaCCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGTGTGCCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCGTTTTCACCGACAAAACCGACCGCGTGGGAgtcgt  >  1:123030/5‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                            TGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTAACGTAAATGCCCTCTTTTTGCAGCTCACGGGCAAATTTCTGCGCCACTACCGCATCACCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGTTCGCTGCCCGCTTCGACCATCTCCAGTACTTTGATGGACGCGGCAACAATGGCCGGTGCCAGCGAGTTGGAGAACAGGTACGGACGAGAACGCTGGCGCAGCCACTCAACCACTTCTTTGCGCGCCGCGGTATAACCACCAGAAGCCCCGCCCAGCGCTTTACCAAGCGTACCGGTGATAATATCGACCCGGCCCATCACATCGCAGTATTCATGGGAACCACGACCATTTTCACCGACAAAACCGACCGCGTGGGAGTCGT  >  NC_000913/3791443‑3791923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑