Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,301,9920AT94.4% 105.6 / ‑5.9 36N271Y (AAT→TAT) oppAoligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (18/16);  minor base C (2/0);  total (20/16)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

TTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGG  >  NC_000913/1301751‑1302230
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                               
ttCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAACGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGc                                                                                                                                                                                                                                         >  2:39216/1‑250 (MQ=255)
ttCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGc                                                                                                                                                                                                                                         >  1:207688/1‑250 (MQ=255)
  cACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGCAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGCATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATg                                                                                                                                                                                                                                      >  2:436762/1‑251 (MQ=255)
  cACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATg                                                                                                                                                                                                                                      >  1:122988/1‑251 (MQ=255)
            aaaaGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAAt                                                                                                                                                                                                                            >  1:273605/1‑251 (MQ=255)
                        cGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATGTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAg                                                                                                                                                                                                                 <  2:113896/250‑1 (MQ=255)
                           gaAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGCTCGAATTGTTCCAGAAGCTg                                                                                                                                                                                                              >  1:394322/1‑250 (MQ=255)
                                     gAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAag                                                                                                                                                                                                       >  2:229173/1‑247 (MQ=255)
                                       aaaaTGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTTAAATCGACATGACGTACAACAGCAGGCCTATCGAATTGTTCCAGAAGCTGAAAAAAGAGATcc                                                                                                                                                                                                 <  2:43315/251‑1 (MQ=255)
                                               cccAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGaa                                                                                                                                                                                         <  2:162727/251‑1 (MQ=255)
                                                 cAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGt                                                                                                                                                                                       >  1:114951/1‑251 (MQ=255)
                                                            aatCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATc                                                                                                                                                                            <  2:71302/250‑1 (MQ=255)
                                                                gTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATa                                                                                                                                                                        <  2:207688/251‑1 (MQ=255)
                                                                  cACCAACGGTGCCTATCCCTTAAAAGATGGGGTCGGAAACGAACGAATCGTTCATGATCGCAGCCCTACCTACTGGAACAACGCGAAAACCGTGATTAACCAGGTAACCTATTTGCCTATTGCATCTGAAGTTACCGATGTTAACCGGTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCTATAcc                                                                                                                                                                      <  1:56493/251‑1 (MQ=255)
                                                                         ggTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCAc                                                                                                                                                               >  1:40085/1‑251 (MQ=255)
                                                                                          aGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAAtt                                                                                                                                               >  1:159432/1‑250 (MQ=255)
                                                                                                          gaacgaaTCGTTCTTGACCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTGACCGATGTCAACCGCTACCGTATTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAACAGATCCCGGACGAAGTTCACGTTGATCCATGCCTGTGCACTTACTATTACGAAATTACCAACCAGAAACCGcc                                                                                                                             <  1:164700/251‑1 (MQ=255)
                                                                                                                     ccgatcccgccgcccgcccTACGGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTGGCCTATTGCTTCTGAAGTTACCAATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTTTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTGGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAt                                                                                                                   <  2:55703/239‑1 (MQ=255)
                                                                                                                            cGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTACCAACCAGAAACCGCCATTCAACGAt                                                                                                                   >  1:55703/1‑243 (MQ=255)
                                                                                                                               aGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAATTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACACCCAGAAACCGCCATTCAACGAtgtgcgtgtgcg                                                                                                        <  2:8409/251‑1 (MQ=255)
                                                                                                                                atctgcccttcTGGAAAAACGCGAAACCCGTTATTACCCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTTCTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtgtgcgtgtgc                                                                                                         <  2:350577/239‑1 (MQ=255)
                                                                                                                                    gACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACTGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCg                                                                                                   >  1:365359/1‑251 (MQ=255)
                                                                                                                                    gACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGAtgtgcgtgtgc                                                                                                         >  1:350577/1‑245 (MQ=255)
                                                                                                                                         aCTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTTCCTATGGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGTTGAAAACGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGATATCCCGGACGAATTTCACGTTGATCCATACCTGTGCACGTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACt                                                                                               <  2:114951/250‑1 (MQ=255)
                                                                                                                                                    ccgAAAACCGTGATAACCCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGAGGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGTTCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTAt                                                                                   <  2:371735/250‑1 (MQ=255)
                                                                                                                                                        aaaCCGTTATTAACCAGGTAACCTCTTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTaac                                                                                                                                            >  2:98050/1‑191 (MQ=255)
                                                                                                                                                        aaaCCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTaac                                                                                                                                            <  1:98050/191‑1 (MQ=255)
                                                                                                                                                         aaCCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGAcc                                                                              >  1:425196/1‑251 (MQ=255)
                                                                                                                                                                                               cggAAGTTACCGATGTCAACCCCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCaa                                         <  2:40085/248‑1 (MQ=255)
                                                                                                                                                                                                 gAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACAt                                      >  1:243144/1‑251 (MQ=255)
                                                                                                                                                                                                    gttacaaaTGTTAACCGCTACCGTAGTGGGGAAATCGACATGACGTATAACAGCTGGCCGATCGAATTGTTCCAGACGCTGAAAAAAGAAATCCCGGACGAAGTTCACGTTGATCCATACCTTTGCACTTACTATTACTAAATTAACAACCAGAATCCGCCATTCAACGATGTGCGTGTGCGTTTCGCACTGAAACTAGGTATGGCCCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGcc                                   <  1:266316/244‑1 (MQ=255)
                                                                                                                                                                                                            tGTCAACCGCTACCGGAGTGGAGAAATCGACATGACGCATGACAGCATAACGATCGAATTGTTACAGGAGCTGAAAACAGTGATACCGGACGTAGTTAACGTGGATCCATAACTGCGCACTTACTATTACGAAATTAACAATCAAAAACCGCCTTTCAACGATGCGCGTGTGCGTACCGCCCTGAAACTAGGTATGGACCGCGATATCATTGTTACTTAAGTGAAAGCGTAGAGCAACATGCCCGCCtctg                           >  2:422734/1‑248 (MQ=255)
                                                                                                                                                                                                                    gCTACCGTAGTGGTGAAATCGACATGACGTATAACAGCATGCCGATCGAATTGATCCAGAAGCTGAAACAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACAATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGAGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACt                   >  1:28192/1‑251 (MQ=255)
                                                                                                                                                                                                                        ccGTAGTGGTGAAATCGACATGACGTATACCAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAAATAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCAc               >  1:316157/1‑251 (MQ=255)
                                                                                                                                                                                                                          gTAGTGGAGAAACCGACATGACGTATAACAGCATGCCGACCAAATTGTCCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGACCCATCCCTGGGCACTTACTATTACGAAATTACAACCCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCg             <  2:139778/251‑1 (MQ=255)
                                                                                                                                                                                                                                     aTCGACATGCCGCATAACAGCCTGCCGATCGAATTGTTCCAGAAGCTGAAAAACGAGCTCCCGGACGAAGTTCACGTTGATCCATCCCTATGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGAGCAGGGCAACATGCCCGACTATGGTTACACTCCACCGCATACTGATgg  >  2:203892/1‑251 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                               
TTCACCGGTGCCAAAAGCCGCTATCGAGAAATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGG  >  NC_000913/1301751‑1302230

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: