Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,791,496	A→G	V352V (GTT→GTC)	kbl ←	2‑amino‑3‑ketobutyrate CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,791,496	0	A	G	100.0%	187.9 / NA	58	V352V (GTT→GTC)	kbl	2‑amino‑3‑ketobutyrate CoA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/24);  total (34/24)

TTTAATCAGCAGATCGTTATGCCCGAGTTCCGGTACAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATCTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTAAATGCTTCTACTGCACGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTAACGTAAATGCCCTCTTTTTGCAGCTCACGGGCAAATTTCTGCGCCACTACCGCATCACCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGTTCGCTGCCCGCTTCGACCATCTCCAGTACTTTGATGGACGCGGCAACAATGGCCGGTGCCAGCGAGTTGGAGAACAG  >  NC_000913/3791253‑3791738                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   tttAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTaa                                                                                                                                                                                                                                              <  1:291350/250‑1 (MQ=255)                       cccaGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACg                                                                                                                                                                                                                       <  1:34418/248‑1 (MQ=255)                       cccaGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACg                                                                                                                                                                                                                       >  1:242402/4‑251 (MQ=255)                         caGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACg                                                                                                                                                                                                                       >  1:218575/2‑249 (MQ=255)                               cggctcAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGGCATCTGGGTACGCATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGAAGTAAGTGCCCTCTTTTTGCAGCTCCCGAGCAAAtt                                                                                                                                                                                                               >  2:425578/6‑251 (MQ=255)                                    cAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTgc                                                                                                                                                                                                          >  1:204681/1‑251 (MQ=255)                                             cGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTAcc                                                                                                                                                                                                 >  2:387391/1‑251 (MQ=255)                                             cGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTAcc                                                                                                                                                                                                 >  2:137151/1‑251 (MQ=255)                                             cGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTAc                                                                                                                                                                                                  <  2:21008/250‑1 (MQ=255)                                              ggTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCg                                                                                                                                                                                                >  1:305744/1‑251 (MQ=255)                                               gTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGc                                                                                                                                                                                               >  2:121210/1‑251 (MQ=255)                                                  aTCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCAGTTCACATCCTCAGGCGATAACTCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATc                                                                                                                                                                                            <  1:391556/251‑1 (MQ=255)                                                  aTCCAGATGCCCTCTGCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATc                                                                                                                                                                                            <  1:217837/251‑1 (MQ=255)                                                       gATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGCCATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGCAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCa                                                                                                                                                                                        >  2:111664/1‑250 (MQ=255)                                                        aTGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCa                                                                                                                                                                                        >  1:351699/1‑249 (MQ=255)                                                          gCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCa                                                                                                                                                                                    <  1:105841/251‑1 (MQ=255)                                                           cccTCTTCCGCTGTCAGTATGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCTCAGTTGTTTACCGATACGCGGGAATGCTTCTACTGCGCGCGTAATGTGCTCACGTGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACCTAAATGCCCTCGTTTTGCAGCGCACGTGCAAATTTCTGCGCCCCATCCGCATCGCTAAGCAt                                                                                                                                                                                   <  2:190518/251‑1 (MQ=255)                                                            ccTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATg                                                                                                                                                                                  >  1:355596/1‑251 (MQ=255)                                                               ctTCCGCTTTCAGTTTGGATAACGCTTTCAGTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTTCTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGTCCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGa                                                                                                                                                                                 <  1:295608/249‑1 (MQ=255)                                                                       ttCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGaata                                                                                                                                                                         <  1:71875/249‑1 (MQ=255)                                                                         cAGTTTGGATAACGCGTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGTCATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATg                                                                                                                                                                      <  2:342062/250‑1 (MQ=255)                                                                          aGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACGGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCGGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCGTTTTGCAGCTCGCGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCAGGACCGGAATCATGGc                                                                                                                                                                    <  2:437765/251‑1 (MQ=255)                                                                          aGTTTGGATAACGCTTTCATTTCACATCATCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGc                                                                                                                                                                    >  2:29643/1‑251 (MQ=255)                                                                           gTTTGGATAACGCTTTCATTTCACATCCTCATGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCGTTTTGCAGCTCACGAGCAAATTTCTGCGCCACGACCGCATCGCCAAGCATGACCGGAATAATGGCg                                                                                                                                                                   <  2:397744/251‑1 (MQ=255)                                                                               ggATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATACTGGCGTGAt                                                                                                                                                               >  1:346802/1‑251 (MQ=255)                                                                                gATAACGCTCTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATc                                                                                                                                                              <  2:188864/251‑1 (MQ=255)                                                                                         ttCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATCCGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTACATGCCCTCTTTTTGCAGATCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGc                                                                                                                                                     >  1:25521/1‑251 (MQ=255)                                                                                         ttCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGc                                                                                                                                                     >  2:354155/1‑251 (MQ=255)                                                                                                 acaTCCTCAGGCGATAACGCCCCGTTGTTTACCAATACGCGGGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTaa                                                                                                                                             <  1:435462/251‑1 (MQ=255)                                                                                                 acaTCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTa                                                                                                                                              >  1:94424/1‑250 (MQ=255)                                                                                                             gATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCGAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGTGTACAGCccgccgccg                                                                                                                                 >  2:559/1‑251 (MQ=255)                                                                                                                   acccAGTTGTTTGCCACTACGCGAGAATGCCTTTACCGCGCGCGTAAATTGCTCAGGGGTATGAGCCGCAGCCAGATGGGTACGAATACGCGGCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCACTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCAAAGCATGACCGGAATAATGGCGAGAACGGCTACAGCCAGGGGCAAGACCGCCGCCGAAAttt                                                                                                                           >  2:51157/2‑251 (MQ=255)                                                                                                                    cccAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTg                                                                                                                          <  2:351699/251‑1 (MQ=255)                                                                                                                           gttTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCGTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCTCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACgg                                                                                                                   <  1:111664/251‑1 (MQ=255)                                                                                                                              ttccAATCCGCGTGAATGCTTCTACTGCGCGCTTAAGTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAACCCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCTTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGaa                                                                                                                 <  2:351726/248‑1 (MQ=255)                                                                                                                                   aTACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGAcg                                                                                                           >  1:424432/1‑251 (MQ=255)                                                                                                                                                cTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGAGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCcac                                                                                              >  1:126536/1‑251 (MQ=255)                                                                                                                                                    tACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGAc                                                                                          >  1:371427/1‑251 (MQ=255)                                                                                                                                                     aCTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAACGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGGTCGCCCACCGACg                                                                                         >  2:358402/1‑251 (MQ=255)                                                                                                                                                                aaTTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTAACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAgct                                                                              >  1:310368/1‑249 (MQ=255)                                                                                                                                                                 aTTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCAGCGCCAAGCATGACCGGAATAATGGCGGGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAgctc                                                                             <  1:78469/251‑4 (MQ=255)                                                                                                                                                                        ccgggtttttcatctcAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCACCACTACCGCATCGCCAAGCATGACCTGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCCCGCAGCTCGCTGccc                                                                      >  1:370345/16‑251 (MQ=255)                                                                                                                                                                                tGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGATGCCCGCTTcaac                                                              >  1:295961/1‑248 (MQ=255)                                                                                                                                                                                      gcAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCCCGCAGCTCGCTGCCCGCTTCCACCAtctc                                                        >  1:297560/1‑251 (MQ=255)                                                                                                                                                                                               tGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACttt                                               >  1:232240/1‑251 (MQ=255)                                                                                                                                                                                                      gAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGa                                         >  1:184645/1‑250 (MQ=255)                                                                                                                                                                                                      gAATACGCGCCTGACCTCTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACAGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGATGCCCGCTTCAACCCTCTCCAGCACTTTAATGGAc                                        >  2:224452/1‑251 (MQ=255)                                                                                                                                                                                                        aTACGCGCCTGACCTTTCGGAACGCCCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTTCAGCTCACTATCAAATTTCTGCGCCACTACCGCATCGCCAATCATGACCGGAATAATTGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGaagc                                      <  1:280897/251‑4 (MQ=255)                                                                                                                                                                                                            gcgcCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGcg                                  <  2:355596/251‑2 (MQ=255)                                                                                                                                                                                                                    acTTTCGGAACGACCGGATAAAAGAAACCGGTGACGCAAATGCCCTCTTTTTGCAACTCACGAGCAAATTTCTGCGCCACTACCGCCTCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGATCGCTGCCCGCTTCAACCATCTCCAGCCCTTTAATGGAAACGGcaacaaaagc                          >  2:166194/2‑247 (MQ=255)                                                                                                                                                                                                                     cTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGcc                         >  1:305663/1‑251 (MQ=255)                                                                                                                                                                                                                            aaCGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGcc                   >  1:160718/1‑250 (MQ=255)                                                                                                                                                                                                                               gACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTg                                                                         >  1:395978/1‑193 (MQ=255)                                                                                                                                                                                                                               gACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTGCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAgcgcgctg                                                                         <  2:395978/193‑8 (MQ=255)                                                                                                                                                                                                                                       agaagaAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCCCCCCTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGACCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGga        <  2:424432/250‑1 (MQ=255)                                                                                                                                                                                                                                       agaagaAACCGGTGACGGAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGgag       <  1:358402/251‑1 (MQ=255)                                                                                                                                                                                                                                        gaagaaACCGTGGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCCCTACCGCACCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGgaga      <  2:305744/251‑1 (MQ=255)                                                                                                                                                                                                                                            aaaCCGGCGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCCCGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGCTCGCTGCCCGCTTCAACCATCTCCAGCACTTTAATGGAAGCGGCGACAATAGCCGGTGCCAGCGAGTTGgagcaccg  >  2:186026/1‑246 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   TTTAATCAGCAGATCGTTATGCCCGAGTTCCGGTACAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATCTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTAAATGCTTCTACTGCACGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTAACGTAAATGCCCTCTTTTTGCAGCTCACGGGCAAATTTCTGCGCCACTACCGCATCACCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACGGTCACGCAGTTCGCTGCCCGCTTCGACCATCTCCAGTACTTTGATGGACGCGGCAACAATGGCCGGTGCCAGCGAGTTGGAGAACAG  >  NC_000913/3791253‑3791738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑