Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,791,406	A→G	R382R (CGT→CGC)	kbl ←	2‑amino‑3‑ketobutyrate CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,791,406	0	A	G	100.0%	179.6 / NA	56	R382R (CGT→CGC)	kbl	2‑amino‑3‑ketobutyrate CoA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/27);  total (29/27)

CACGACCATCGGCACCGGGATGGTTTTTTGCGACCACTCATCCCAGTTATAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATCAGCAGATCGTTATGCCCGAGTTCCGG‑TACAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATCTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTAAATGCTTCTACTGCACGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTAACGTAAATGCCCTCTTTTTGCAGCTCACGGGCAAATTTCTGCGCCACTACCGCATCACCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACG  >  NC_000913/3791163‑3791651                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      gacaaCCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTTTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCTTTATTACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCATATGCCCGCTTCCGCTTTCAGTTTGGAGAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTaa                                                                                                                                                                                                                                                <  1:173393/247‑1 (MQ=255)  acaaCCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAAt                                                                                                                                                                                                                                               <  2:193968/248‑1 (MQ=255)                   gatagTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAgggg                                                                                                                                                                                                                                    >  1:419099/5‑245 (MQ=255)                   gatagTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAgggg                                                                                                                                                                                                                                    <  2:419099/241‑1 (MQ=255)                      agttgttttcgacctcgcATCCCATTTGTAGATGTGGACGTCAGTTCCGCAGATGGCTGTGGTACGGATTTAAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTGCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCTCGTAATTTGCTCAGGTGTATGcgccg                                                                                                                                                                                                                           <  2:177353/234‑1 (MQ=255)                              gCGACAACTCATCCCAGTTGTAGATGTGACCGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTCTGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCACAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATc                                                                                                                                                                                                                   >  2:263367/1‑251 (MQ=255)                                   cACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTgggg                                                                                                                                                                                                              >  1:257088/1‑250 (MQ=255)                                       cATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTAc                                                                                                                                                                                                            <  1:263367/249‑1 (MQ=255)                                          cccAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATa                                                                                                                                                                                                       <  1:10120/251‑1 (MQ=255)                                            cAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGGTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCTCCGCAGACATCTGGGTACGAATAcg                                                                                                                                                                                                     <  2:226537/251‑1 (MQ=255)                                                                    ccGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCgg                                                                                                                                                                             <  1:29941/251‑1 (MQ=255)                                                                      gCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGCTAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGACTACGCGCCTGACCTTTCGGAACGACCGGAt                                                                                                                                                                           >  1:367787/1‑251 (MQ=255)                                                                            ggCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTA‑AGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATaga                                                                                                                                                                        >  1:382134/1‑248 (MQ=255)                                                                            ggCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATaga                                                                                                                                                                        >  1:334403/1‑248 (MQ=255)                                                                                      cGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTTGATAACGCTTTCATTTCACATCCTCATGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGtgac                                                                                                                                                           <  2:73838/251‑4 (MQ=255)                                                                                           tttAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTaa                                                                                                                                                       <  1:291350/250‑1 (MQ=255)                                                                                                                acccaGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCgg                                                                                                                                                                             >  2:170631/5‑207 (MQ=255)                                                                                                                acccaGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCgg                                                                                                                                                                             <  1:170631/203‑1 (MQ=255)                                                                                                                 cccaGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACg                                                                                                                                >  1:242402/4‑251 (MQ=255)                                                                                                                 cccaGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACg                                                                                                                                <  1:34418/248‑1 (MQ=255)                                                                                                                   caGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACg                                                                                                                                >  1:218575/2‑249 (MQ=255)                                                                                                                          cggctcAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGGCATCTGGGTACGCATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGAAGTAAGTGCCCTCTTTTTGCAGCTCCCGAGCAAAtt                                                                                                                        >  2:425578/6‑251 (MQ=255)                                                                                                                               cAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTgc                                                                                                                   >  1:204681/1‑251 (MQ=255)                                                                                                                                        cGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTAcc                                                                                                          >  2:387391/1‑251 (MQ=255)                                                                                                                                        cGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTAcc                                                                                                          >  2:137151/1‑251 (MQ=255)                                                                                                                                        cGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTAc                                                                                                           <  2:21008/250‑1 (MQ=255)                                                                                                                                         ggTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCg                                                                                                         >  1:305744/1‑251 (MQ=255)                                                                                                                                          gTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGc                                                                                                        >  2:121210/1‑251 (MQ=255)                                                                                                                                             aTCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCAGTTCACATCCTCAGGCGATAACTCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATc                                                                                                     <  1:391556/251‑1 (MQ=255)                                                                                                                                             aTCCAGATGCCCTCTGCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATc                                                                                                     <  1:217837/251‑1 (MQ=255)                                                                                                                                                  gATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGCCATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGCAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCa                                                                                                 >  2:111664/1‑250 (MQ=255)                                                                                                                                                   aTGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCa                                                                                                 >  1:351699/1‑249 (MQ=255)                                                                                                                                                     gCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCa                                                                                             <  1:105841/251‑1 (MQ=255)                                                                                                                                                      cccTCTTCCGCTGTCAGTATGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCTCAGTTGTTTACCGATACGCGGGAATGCTTCTACTGCGCGCGTAATGTGCTCACGTGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACCTAAATGCCCTCGTTTTGCAGCGCACGTGCAAATTTCTGCGCCCCATCCGCATCGCTAAGCAt                                                                                            <  2:190518/251‑1 (MQ=255)                                                                                                                                                       ccTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATg                                                                                           >  1:355596/1‑251 (MQ=255)                                                                                                                                                          ctTCCGCTTTCAGTTTGGATAACGCTTTCAGTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTTCTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGTCCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGa                                                                                          <  1:295608/249‑1 (MQ=255)                                                                                                                                                                  ttCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGaata                                                                                  <  1:71875/249‑1 (MQ=255)                                                                                                                                                                    cAGTTTGGATAACGCGTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGTCATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATg                                                                               <  2:342062/250‑1 (MQ=255)                                                                                                                                                                     aGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACGGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCGGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCGTTTTGCAGCTCGCGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCAGGACCGGAATCATGGc                                                                             <  2:437765/251‑1 (MQ=255)                                                                                                                                                                     aGTTTGGATAACGCTTTCATTTCACATCATCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGc                                                                             >  2:29643/1‑251 (MQ=255)                                                                                                                                                                      gTTTGGATAACGCTTTCATTTCACATCCTCATGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCGTTTTGCAGCTCACGAGCAAATTTCTGCGCCACGACCGCATCGCCAAGCATGACCGGAATAATGGCg                                                                            <  2:397744/251‑1 (MQ=255)                                                                                                                                                                          ggATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATACTGGCGTGAt                                                                        >  1:346802/1‑251 (MQ=255)                                                                                                                                                                           gATAACGCTCTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATc                                                                       <  2:188864/251‑1 (MQ=255)                                                                                                                                                                                    ttCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATCCGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTACATGCCCTCTTTTTGCAGATCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGc                                                              >  1:25521/1‑251 (MQ=255)                                                                                                                                                                                    ttCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGc                                                              >  2:354155/1‑251 (MQ=255)                                                                                                                                                                                            acaTCCTCAGGCGATAACGCCCCGTTGTTTACCAATACGCGGGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTaa                                                      <  1:435462/251‑1 (MQ=255)                                                                                                                                                                                            acaTCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTa                                                       >  1:94424/1‑250 (MQ=255)                                                                                                                                                                                                        gATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCGAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGTGTACAGCccgccgccg                                          >  2:559/1‑251 (MQ=255)                                                                                                                                                                                                              acccAGTTGTTTGCCACTACGCGAGAATGCCTTTACCGCGCGCGTAAATTGCTCAGGGGTATGAGCCGCAGCCAGATGGGTACGAATACGCGGCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCACTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCAAAGCATGACCGGAATAATGGCGAGAACGGCTACAGCCAGGGGCAAGACCGCCGCCGAAAttt                                    >  2:51157/2‑251 (MQ=255)                                                                                                                                                                                                               cccAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTg                                   <  2:351699/251‑1 (MQ=255)                                                                                                                                                                                                                      gttTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCGTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCTCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACgg                            <  1:111664/251‑1 (MQ=255)                                                                                                                                                                                                                         ttccAATCCGCGTGAATGCTTCTACTGCGCGCTTAAGTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAACCCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCTTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGaa                          <  2:351726/248‑1 (MQ=255)                                                                                                                                                                                                                              aTACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGAcg                    >  1:424432/1‑251 (MQ=255)                                                                                                                                                                                                                                           cTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGAGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCcac       >  1:126536/1‑251 (MQ=255)                                                                                                                                                                                                                                               tACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGAc   >  1:371427/1‑251 (MQ=255)                                                                                                                                                                                                                                                aCTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTAAATGCCCTCTTTTTGCAGCTCACGAGCAAATTTCTGCGCCACTACCGCATCGCCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAACGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGGTCGCCCACCGACg  >  2:358402/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      CACGACCATCGGCACCGGGATGGTTTTTTGCGACCACTCATCCCAGTTATAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATCAGCAGATCGTTATGCCCGAGTTCCGG‑TACAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATCTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTAAATGCTTCTACTGCACGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTAACGTAAATGCCCTCTTTTTGCAGCTCACGGGCAAATTTCTGCGCCACTACCGCATCACCAAGCATGACCGGAATAATGGCGTGATCGGCTCCCGCCAGGGTAAAGCCCGCCGCCGACATTTGCTCACGGAACTGACGCGCGTTCGCCCACAGACG  >  NC_000913/3791163‑3791651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑