Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,791,277	G→C	L23L (CTC→CTG)	tdh ←	threonine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,791,277	0	G	C	100.0%	185.6 / NA	52	L23L (CTC→CTG)	tdh	threonine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (22/30);  total (22/30)

GGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCACTTCACCGACATATTCATGGCCCACGACCATCGGCACCGGGATGGTTTTTTGCGACCACTCATCCCAGTTATAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATCAGCAGATCGTTATGCCCGAGTTCCGG‑TACAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATCTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTAAATGCTTCTACTGCACGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTAACGTAA  >  NC_000913/3791034‑3791502                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                   ggTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGACTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCg                                                                                                                                                                                                                             >  1:190518/1‑251 (MQ=255)     ggtccgccacGACAGTTGCGACAGGGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCggctc                                                                                                                                                                                                                         <  2:290646/245‑4 (MQ=255)        ccgcctcgaaaGTTGCGACAGTGACCACAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCCCTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCggctcagg                                                                                                                                                                                                                      <  2:349989/241‑7 (MQ=255)              cGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTc                                                                                                                                                                                                               >  1:406818/1‑251 (MQ=255)                              gACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGccc                                                                                                                                                                                               <  2:163124/251‑1 (MQ=255)                                     ggTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGAAGCCCTCTTCCg                                                                                                                                                                                        <  1:339498/251‑1 (MQ=255)                                                cTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTg                                                                                                                                                                             >  1:397744/1‑251 (MQ=255)                                                     ccagcgccgcgATCGCCATTCTTAAAGCCTTTCAATTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTT‑ACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATacc                                                                                                                                                                       >  2:295608/8‑249 (MQ=255)                                                          gacgcgagagccaataggaaaGCCTTTCACGTCCTGACTGAGTCCTGCTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTGTTGAGACCACTCATCACAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTGCCGCTTTCAGTTTGGATAACGCtt                                                                                                                                                                   <  2:157665/232‑1 (MQ=255)                                                            cgcgATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTc                                                                                                                                                                 >  2:105841/1‑251 (MQ=255)                                                                   gccaaTCTTAAATCCTTTCACTTCCTGACCGATGCCTACTACTTCTCCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCTCAGATGGCTGTTTTACGGATTTTAAGTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTGTCAGTTTGGATAACGCTTGCATTTcac                                                                                                                                                          <  2:249111/247‑1 (MQ=255)                                                                     caaTCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATGTCACAt                                                                                                                                                        <  2:399815/249‑1 (MQ=255)                                                                       aTCTTAAAGCCTTTCACTTCATGACCGATGCCTACTACTTCACCGCCATATTCATGACCGACAAACCTCGGCACCGGGATAGTTTTTTGCGACCCCTCATCCCAGTTGTCGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCCGTTTGGATAACGCTTTCATTTCACATcc                                                                                                                                                      >  2:209266/1‑251 (MQ=255)                                                                            aaaGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTTTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACTCTTGCATGTCACATCCTCAg                                                                                                                                                  <  1:383074/249‑1 (MQ=255)                                                                                ccTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGAt                                                                                                                                             <  2:44232/251‑1 (MQ=255)                                                                                 cTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGAt                                                                                                                                             >  1:109202/1‑250 (MQ=255)                                                                                  tttCACTTCCTGACCGTTGCCTACTACTTCACCGACATATTCATGACCGTCAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCCTCCCAGTTGTAGGTGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGTTTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACCTCGGTCATCCAGATGCCCTCTTCCGCTTTGAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGaca                                                                                                                                            >  2:14101/1‑248 (MQ=255)                                                                                     cacgtccttaccgacgcctaatacgtCACCGACATATTCAGGACCGACAACCATCGGCACCGGGATAGTGTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGGCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCTGTATGACGCAGTTCCGG‑CTTATGAACGTCGGTCATCCAGATGCCCTCTTCCGTTTTCAGTTTGGATAGCGCTTTCATTTCACATTCTCAGGCGTTAACg                                                                                                                                         <  2:61567/225‑1 (MQ=255)                                                                                         tCCTGACCTATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCCCTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGTCCTCGTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACAGCCTCAGGCGATAACGCCCa                                                                                                                                     <  2:156639/250‑1 (MQ=255)                                                                                             gaccgatgcctactACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAgttgt                                                                                                                                >  2:71875/9‑251 (MQ=255)                                                                                             gaccgatgcctactACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAgttgt                                                                                                                                <  1:287387/243‑1 (MQ=255)                                                                                             gaccgatgcctactACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAgttg                                                                                                                                 <  2:406818/242‑1 (MQ=255)                                                                                                cgatgcctactACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGttt                                                                                                                              >  2:317588/6‑250 (MQ=255)                                                                                                 gatgcctactACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGAGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCACAGTTGTTTAc                                                                                                                            >  2:245698/5‑251 (MQ=255)                                                                                                   tgcctactccTTCACCGAAATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGCTAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTAcc                                                                                                                           >  2:78469/10‑250 (MQ=255)                                                                                                       tactaCTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATAc                                                                                                                      >  1:193968/5‑251 (MQ=255)                                                                                                        actaCTTCACCGACATATTCATGACCGACATCCATCGGCACCGGGATTGTTTTTTGCGACCTCTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATAcg                                                                                                                     <  2:141177/248‑1 (MQ=255)                                                                                                               cACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCGCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAAt                                                                                                              <  1:209266/251‑1 (MQ=255)                                                                                                                 ccGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGc                                                                                                            >  1:21008/1‑251 (MQ=255)                                                                                                                   gACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCtt                                                                                                          <  2:228864/251‑1 (MQ=255)                                                                                                                   gACATAGTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCtt                                                                                                          <  2:418146/251‑1 (MQ=255)                                                                                                                     cATATTCATGACCGACACCCATCGGCACCGTGATAGTTTTTTGCGACCACTCCTCCCAGTTGTAGATGTTATCGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAAATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCt                                                                                                        <  1:57572/251‑1 (MQ=255)                                                                                                                      atatTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAAc                                                                                                                                          >  1:246572/1‑216 (MQ=255)                                                                                                                      atatTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAAc                                                                                                                                          <  2:246572/216‑1 (MQ=255)                                                                                                                       tatTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCCGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTAc                                                                                                      >  2:291350/1‑251 (MQ=255)                                                                                                                        atTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACt                                                                                                     <  2:7300/251‑1 (MQ=255)                                                                                                                                  gacaaCCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTTTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCTTTATTACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCATATGCCCGCTTCCGCTTTCAGTTTGGAGAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTaa                                                                                           <  1:173393/247‑1 (MQ=255)                                                                                                                                   acaaCCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAAt                                                                                          <  2:193968/248‑1 (MQ=255)                                                                                                                                                    gatagTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAgggg                                                                               >  1:419099/5‑245 (MQ=255)                                                                                                                                                    gatagTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAgggg                                                                               <  2:419099/241‑1 (MQ=255)                                                                                                                                                       agttgttttcgacctcgcATCCCATTTGTAGATGTGGACGTCAGTTCCGCAGATGGCTGTGGTACGGATTTAAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTGCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCTCGTAATTTGCTCAGGTGTATGcgccg                                                                      <  2:177353/234‑1 (MQ=255)                                                                                                                                                               gCGACAACTCATCCCAGTTGTAGATGTGACCGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTCTGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCACAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATc                                                              >  2:263367/1‑251 (MQ=255)                                                                                                                                                                    cACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTgggg                                                         >  1:257088/1‑250 (MQ=255)                                                                                                                                                                        cATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTAc                                                       <  1:263367/249‑1 (MQ=255)                                                                                                                                                                           cccAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATa                                                  <  1:10120/251‑1 (MQ=255)                                                                                                                                                                             cAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGGTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCTCCGCAGACATCTGGGTACGAATAcg                                                <  2:226537/251‑1 (MQ=255)                                                                                                                                                                                                     ccGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCgg                        <  1:29941/251‑1 (MQ=255)                                                                                                                                                                                                       gCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGCTAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGACTACGCGCCTGACCTTTCGGAACGACCGGAt                      >  1:367787/1‑251 (MQ=255)                                                                                                                                                                                                             ggCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTA‑AGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATaga                   >  1:382134/1‑248 (MQ=255)                                                                                                                                                                                                             ggCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATaga                   >  1:334403/1‑248 (MQ=255)                                                                                                                                                                                                                       cGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTTGATAACGCTTTCATTTCACATCCTCATGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGtgac      <  2:73838/251‑4 (MQ=255)                                                                                                                                                                                                                            tttAATTAGCAGATCGTTATGACCCAGTTCCGG‑CTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACTGCGCGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTGACGTaa  <  1:291350/250‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                   GGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCACTTCACCGACATATTCATGGCCCACGACCATCGGCACCGGGATGGTTTTTTGCGACCACTCATCCCAGTTATAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATCAGCAGATCGTTATGCCCGAGTTCCGG‑TACAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATCTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTAAATGCTTCTACTGCACGCGTAATTTGCTCAGGGGTATGCGCCGCAGACATCTGGGTACGAATACGCGCCTGACCTTTCGGAACGACCGGATAGAAGAAACCGGTAACGTAA  >  NC_000913/3791034‑3791502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑