Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,791,163	C→G	V61V (GTG→GTC)	tdh ←	threonine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,791,163	0	C	G	100.0%	139.2 / NA	44	V61V (GTG→GTC)	tdh	threonine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (18/26);  total (18/26)

CCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCACTTCACCGACATATTCATGGCCCACGACCATCGGCACCGGGATGGTTTTTTGCGACCACTCATCCCAGTTATAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATCAGCAGATCGTTATGCCCGAGTTCCGGTACAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATCTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTAAATGCTTCTACT  >  NC_000913/3790991‑3791403                                                                                                                                                                             |                                                                                                                                                                                                                                                 cgggacggtgaacgccggcGCCGATCGTGTTGCGCCAGAGAGGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCAGGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGc                                                                                                                                                                     <  2:47798/232‑1 (MQ=255)      gggttaacgccgacgcggaTCGGGTTGCGGCACAGAGGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCAGCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGtttt                                                                                                                                                               <  1:162589/233‑1 (MQ=255)        gtcaccgccaccgccgacggtgtGGCGGCACAGATGGTTACGCCCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTAc                                                                                                                                                             <  2:332659/232‑1 (MQ=255)           aacgccgaCGCCGATCGTGTTGGGGCAAAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGa                                                                                                                                                          <  1:318795/244‑1 (MQ=255)            acgccgaccccgtggtgtttGCGGAACATATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGAt                                                                                                                                                         <  1:138650/233‑1 (MQ=255)                    gccgaTCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAAtt                                                                                                                                                  >  1:351726/5‑249 (MQ=255)                      cgaTCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATtagc                                                                                                                                               >  1:7300/3‑251 (MQ=255)                              tcgcgccacaggtgcatcctgccgccacGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTTCTACACCGACATATTCAGGACCGTCAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGCTCGt                                                                                                                                        <  2:436395/226‑1 (MQ=255)                                            ggTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGACTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCg                                                                                                                         >  1:190518/1‑251 (MQ=255)                                                ggtccgccacGACAGTTGCGACAGGGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCggctc                                                                                                                     <  2:290646/245‑4 (MQ=255)                                                   ccgcctcgaaaGTTGCGACAGTGACCACAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCCCTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCggctcagg                                                                                                                  <  2:349989/241‑7 (MQ=255)                                                         cGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTc                                                                                                            >  1:406818/1‑251 (MQ=255)                                                                         gACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGccc                                                                                            <  2:163124/251‑1 (MQ=255)                                                                                ggTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGAAGCCCTCTTCCg                                                                                     <  1:339498/251‑1 (MQ=255)                                                                                           cTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTg                                                                          >  1:397744/1‑251 (MQ=255)                                                                                                ccagcgccgcgATCGCCATTCTTAAAGCCTTTCAATTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTT‑ACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATacc                                                                    >  2:295608/8‑249 (MQ=255)                                                                                                     gacgcgagagccaataggaaaGCCTTTCACGTCCTGACTGAGTCCTGCTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTGTTGAGACCACTCATCACAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTGCCGCTTTCAGTTTGGATAACGCtt                                                                <  2:157665/232‑1 (MQ=255)                                                                                                       cgcgATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTc                                                              >  2:105841/1‑251 (MQ=255)                                                                                                              gccaaTCTTAAATCCTTTCACTTCCTGACCGATGCCTACTACTTCTCCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCTCAGATGGCTGTTTTACGGATTTTAAGTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTGTCAGTTTGGATAACGCTTGCATTTcac                                                       <  2:249111/247‑1 (MQ=255)                                                                                                                caaTCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATGTCACAt                                                     <  2:399815/249‑1 (MQ=255)                                                                                                                  aTCTTAAAGCCTTTCACTTCATGACCGATGCCTACTACTTCACCGCCATATTCATGACCGACAAACCTCGGCACCGGGATAGTTTTTTGCGACCCCTCATCCCAGTTGTCGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCCGTTTGGATAACGCTTTCATTTCACATcc                                                   >  2:209266/1‑251 (MQ=255)                                                                                                                       aaaGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTTTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACTCTTGCATGTCACATCCTCAg                                               <  1:383074/249‑1 (MQ=255)                                                                                                                           ccTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGAt                                          <  2:44232/251‑1 (MQ=255)                                                                                                                            cTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGAt                                          >  1:109202/1‑250 (MQ=255)                                                                                                                             tttCACTTCCTGACCGTTGCCTACTACTTCACCGACATATTCATGACCGTCAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCCTCCCAGTTGTAGGTGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGTTTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACCTCGGTCATCCAGATGCCCTCTTCCGCTTTGAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGaca                                         >  2:14101/1‑248 (MQ=255)                                                                                                                                cacgtccttaccgacgcctaatacgtCACCGACATATTCAGGACCGACAACCATCGGCACCGGGATAGTGTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGGCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCTGTATGACGCAGTTCCGGCTTATGAACGTCGGTCATCCAGATGCCCTCTTCCGTTTTCAGTTTGGATAGCGCTTTCATTTCACATTCTCAGGCGTTAACg                                      <  2:61567/225‑1 (MQ=255)                                                                                                                                    tCCTGACCTATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCCCTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGTCCTCGTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACAGCCTCAGGCGATAACGCCCa                                  <  2:156639/250‑1 (MQ=255)                                                                                                                                        gaccgatgcctactACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAgttgt                             <  1:287387/243‑1 (MQ=255)                                                                                                                                        gaccgatgcctactACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAgttgt                             >  2:71875/9‑251 (MQ=255)                                                                                                                                        gaccgatgcctactACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAgttg                              <  2:406818/242‑1 (MQ=255)                                                                                                                                           cgatgcctactACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGttt                           >  2:317588/6‑250 (MQ=255)                                                                                                                                            gatgcctactACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGAGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCACAGTTGTTTAc                         >  2:245698/5‑251 (MQ=255)                                                                                                                                              tgcctactccTTCACCGAAATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGCTAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTAcc                        >  2:78469/10‑250 (MQ=255)                                                                                                                                                  tactaCTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATAc                   >  1:193968/5‑251 (MQ=255)                                                                                                                                                   actaCTTCACCGACATATTCATGACCGACATCCATCGGCACCGGGATTGTTTTTTGCGACCTCTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATAcg                  <  2:141177/248‑1 (MQ=255)                                                                                                                                                          cACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCGCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAAt           <  1:209266/251‑1 (MQ=255)                                                                                                                                                            ccGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGc         >  1:21008/1‑251 (MQ=255)                                                                                                                                                              gACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCtt       <  2:228864/251‑1 (MQ=255)                                                                                                                                                              gACATAGTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCtt       <  2:418146/251‑1 (MQ=255)                                                                                                                                                                cATATTCATGACCGACACCCATCGGCACCGTGATAGTTTTTTGCGACCACTCCTCCCAGTTGTAGATGTTATCGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAAATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCt     <  1:57572/251‑1 (MQ=255)                                                                                                                                                                 atatTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAAc                                       <  2:246572/216‑1 (MQ=255)                                                                                                                                                                 atatTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAAc                                       >  1:246572/1‑216 (MQ=255)                                                                                                                                                                  tatTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCCGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTAc   >  2:291350/1‑251 (MQ=255)                                                                                                                                                                   atTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATTTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTGAATGCTTCTACt  <  2:7300/251‑1 (MQ=255)                                                                                                                                                                             |                                                                                                                                                                                                                                                 CCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCACTTCACCGACATATTCATGGCCCACGACCATCGGCACCGGGATGGTTTTTTGCGACCACTCATCCCAGTTATAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATCAGCAGATCGTTATGCCCGAGTTCCGGTACAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATCTCACATCCTCAGGCGATAACGCCCAGTTGTTTACCAATACGCGTAAATGCTTCTACT  >  NC_000913/3790991‑3791403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑