Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,791,109	G→A	F79F (TTC→TTT)	tdh ←	threonine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,791,109	0	G	A	100.0%	70.1 / NA	22	F79F (TTC→TTT)	tdh	threonine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/12);  total (10/12)

ACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCACTTCACCGACATATTCATGGCCCACGACCATCGGCACCGGGATGGTTTTTTGCGACCACTCATCCCAGTTATAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATCAGCAGATCGTTATGCCCGAGTTCCGGTACAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATCTCACATCC  >  NC_000913/3790871‑3791354                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                      aCAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACt                                                                                                                                                                                                                                           >  2:126116/1‑251 (MQ=255)           aTGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGCTCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGAccg                                                                                                                                                                                                                                  >  2:161221/1‑249 (MQ=255)                                                                                                                         cgggacggtgaacgccggcGCCGATCGTGTTGCGCCAGAGAGGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCAGGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGc                                                                                                                    <  2:47798/232‑1 (MQ=255)                                                                                                                              gggttaacgccgacgcggaTCGGGTTGCGGCACAGAGGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCAGCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGtttt                                                                                                              <  1:162589/233‑1 (MQ=255)                                                                                                                                gtcaccgccaccgccgacggtgtGGCGGCACAGATGGTTACGCCCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTAc                                                                                                            <  2:332659/232‑1 (MQ=255)                                                                                                                                   aacgccgaCGCCGATCGTGTTGGGGCAAAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGa                                                                                                         <  1:318795/244‑1 (MQ=255)                                                                                                                                    acgccgaccccgtggtgtttGCGGAACATATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGAt                                                                                                        <  1:138650/233‑1 (MQ=255)                                                                                                                                            gccgaTCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAAtt                                                                                                 >  1:351726/5‑249 (MQ=255)                                                                                                                                              cgaTCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATtagc                                                                                              >  1:7300/3‑251 (MQ=255)                                                                                                                                                      tcgcgccacaggtgcatcctgccgccacGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTTCTACACCGACATATTCAGGACCGTCAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGCTCGt                                                                                       <  2:436395/226‑1 (MQ=255)                                                                                                                                                                    ggTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGACTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCg                                                                        >  1:190518/1‑251 (MQ=255)                                                                                                                                                                        ggtccgccacGACAGTTGCGACAGGGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCggctc                                                                    <  2:290646/245‑4 (MQ=255)                                                                                                                                                                           ccgcctcgaaaGTTGCGACAGTGACCACAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCCCTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCggctcagg                                                                 <  2:349989/241‑7 (MQ=255)                                                                                                                                                                                 cGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTc                                                           >  1:406818/1‑251 (MQ=255)                                                                                                                                                                                                 gACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGccc                                           <  2:163124/251‑1 (MQ=255)                                                                                                                                                                                                        ggTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGAAGCCCTCTTCCg                                    <  1:339498/251‑1 (MQ=255)                                                                                                                                                                                                                   cTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTg                         >  1:397744/1‑251 (MQ=255)                                                                                                                                                                                                                        ccagcgccgcgATCGCCATTCTTAAAGCCTTTCAATTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTT‑ACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATacc                   >  2:295608/8‑249 (MQ=255)                                                                                                                                                                                                                               cgcgATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTc             >  2:105841/1‑251 (MQ=255)                                                                                                                                                                                                                                      gccaaTCTTAAATCCTTTCACTTCCTGACCGATGCCTACTACTTCTCCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCTCAGATGGCTGTTTTACGGATTTTAAGTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTGTCAGTTTGGATAACGCTTGCATTTcac      <  2:249111/247‑1 (MQ=255)                                                                                                                                                                                                                                        caaTCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATGTCACAt    <  2:399815/249‑1 (MQ=255)                                                                                                                                                                                                                                          aTCTTAAAGCCTTTCACTTCATGACCGATGCCTACTACTTCACCGCCATATTCATGACCGACAAACCTCGGCACCGGGATAGTTTTTTGCGACCCCTCATCCCAGTTGTCGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCGGCTCAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCCGTTTGGATAACGCTTTCATTTCACATcc  >  2:209266/1‑251 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                      ACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCACTTCACCGACATATTCATGGCCCACGACCATCGGCACCGGGATGGTTTTTTGCGACCACTCATCCCAGTTATAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATCAGCAGATCGTTATGCCCGAGTTCCGGTACAGGAACGTCGGTCATCCAGATGCCCTCTTCCGCTTTCAGTTTGGATAACGCTTTCATCTCACATCC  >  NC_000913/3790871‑3791354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑