Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,791,043	A→G	G101G (GGT→GGC)	tdh ←	threonine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,791,043	0	A	G	100.0%	58.8 / NA	18	G101G (GGT→GGC)	tdh	threonine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/5);  total (13/5)

GCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCACTTCACCGACATATTCATGGCCCACGACCATCGGCACCGGGATGGTTTTTTGCGACCACTCATCCCAGTTATAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATCAGCAGATCGTTATGCCCGAGTTCCG  >  NC_000913/3790798‑3791284                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  gCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGGACGACCGCCACg                                                                                                                                                                                                                                              >  1:73838/1‑251 (MQ=255)                      cctaTCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGCAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGAccgc                                                                                                                                                                                                                          >  1:418146/4‑248 (MQ=255)                            ggaccTGCACCAGTGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCgacagggaccgcaggggat                                                                                                                                                                                                                   >  2:138650/4‑233 (MQ=255)                             gaccTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGat                                                                                                                                                                                                                   >  1:399815/3‑250 (MQ=255)                                   cACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCt                                                                                                                                                                                                            >  2:10120/1‑251 (MQ=255)                                                  caTCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGCAGGGATCAAAAATGGACGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGAcgcg                                                                                                                                                                                              >  2:162685/1‑250 (MQ=255)                                                     cctCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCg                                                                                                                                                                                          >  1:44232/3‑251 (MQ=255)                                                           caaccaGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATc                                                                                                                                                                                    >  1:188864/3‑251 (MQ=255)                                                                          aCAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACt                                                                                                                                                                     >  2:126116/1‑251 (MQ=255)                                                                                    aTGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGCTCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGAccg                                                                                                                                                            >  2:161221/1‑249 (MQ=255)                                                                                                                                                                                                  cgggacggtgaacgccggcGCCGATCGTGTTGCGCCAGAGAGGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCAGGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGc                                              <  2:47798/232‑1 (MQ=255)                                                                                                                                                                                                       gggttaacgccgacgcggaTCGGGTTGCGGCACAGAGGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCAGCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGtttt                                        <  1:162589/233‑1 (MQ=255)                                                                                                                                                                                                         gtcaccgccaccgccgacggtgtGGCGGCACAGATGGTTACGCCCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTAc                                      <  2:332659/232‑1 (MQ=255)                                                                                                                                                                                                            aacgccgaCGCCGATCGTGTTGGGGCAAAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGa                                   <  1:318795/244‑1 (MQ=255)                                                                                                                                                                                                             acgccgaccccgtggtgtttGCGGAACATATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGAt                                  <  1:138650/233‑1 (MQ=255)                                                                                                                                                                                                                     gccgaTCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAAtt                           >  1:351726/5‑249 (MQ=255)                                                                                                                                                                                                                       cgaTCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATtagc                        >  1:7300/3‑251 (MQ=255)                                                                                                                                                                                                                                             ggTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGACTTTCACTTCCTGACCGATGCCTACTACTTCACCGACATATTCATGACCGACAACCATCGGCACCGGGATAGTTTTTTGCGACCACTCATCCCAGTTGTAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATTAGCAGATCGTTATGACCCAGTTCCg  >  1:190518/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  GCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCAATACCTACCACTTCACCGACATATTCATGGCCCACGACCATCGGCACCGGGATGGTTTTTTGCGACCACTCATCCCAGTTATAGATGTGAACGTCAGTCCCGCAGATGGCTGTTTTACGGATTTTAATCAGCAGATCGTTATGCCCGAGTTCCG  >  NC_000913/3790798‑3791284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑