Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,790,995	G→A	R117R (CGC→CGT)	tdh ←	threonine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,790,995	0	G	A	100.0%	59.1 / NA	22	R117R (CGC→CGT)	tdh	threonine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/6);  total (16/6)

CGTTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCA  >  NC_000913/3790754‑3791130                                                                                                                                                                                                                                                  |                                                                                                                                        cattAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAacg                                                                                                                                >  2:318795/3‑250 (MQ=255)     accaacAGTGATCACCACATTGCGTCCGTCATCGTGTTTCGCCTCCGCCGCAGCCATAATACCTATCGGATCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAacgccga                                                                                                                            <  1:151002/246‑6 (MQ=255)           aGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCACGGACGGTTAACGCCGACGccg                                                                                                                       >  1:332659/1‑249 (MQ=255)            gTGATCACCACAGTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGccgat                                                                                                                     <  1:396238/251‑4 (MQ=255)               aTCACCACATTGCGTGCCCCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCTGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCgt                                                                                                                  <  1:74472/251‑1 (MQ=255)               aTCACCACATTGCGTGCACCCAAGTGATTCGCAACCGCCGCAGCAATCATACCTACCGGACCTGCACCAGAGCCCAGCACATCCTCGCCACCCAGCTCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGCTCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCgt                                                                                                                  >  2:383074/1‑251 (MQ=255)                                ccaaaCGGGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATggg                                                                                                 <  2:187316/248‑1 (MQ=255)                                aCCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATc                                                                                                                    <  1:45921/232‑1 (MQ=255)                                aCCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATc                                                                                                                    >  2:45921/1‑232 (MQ=255)                                    aCGTGTTCCCCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACg                                                                                             <  2:96581/251‑1 (MQ=255)                                      gtgtTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACGac                                                                                           >  2:121527/1‑251 (MQ=255)                                      gtgtTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACGac                                                                                           >  1:226537/1‑251 (MQ=255)                                             gCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGGACGACCGCCACg                                                                                    >  1:73838/1‑251 (MQ=255)                                                                  cctaTCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGCAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGAccgc                                                                >  1:418146/4‑248 (MQ=255)                                                                        ggaccTGCACCAGTGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCgacagggaccgcaggggat                                                         >  2:138650/4‑233 (MQ=255)                                                                         gaccTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGat                                                         >  1:399815/3‑250 (MQ=255)                                                                               cACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCt                                                  >  2:10120/1‑251 (MQ=255)                                                                                              caTCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGCAGGGATCAAAAATGGACGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGAcgcg                                    >  2:162685/1‑250 (MQ=255)                                                                                                 cctCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCg                                >  1:44232/3‑251 (MQ=255)                                                                                                       caaccaGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATc                          >  1:188864/3‑251 (MQ=255)                                                                                                                      aCAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACt           >  2:126116/1‑251 (MQ=255)                                                                                                                                aTGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGCTCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGAccg  >  2:161221/1‑249 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                        CGTTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCA  >  NC_000913/3790754‑3791130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑