Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,790,917	T→C	L143M (TTA→CTG)	tdh ←	threonine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,790,917	0	T	C	100.0%	130.6 / NA	36	L143M (TTA→CTG)	tdh	threonine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/13);  total (23/13)

TGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCA  >  NC_000913/3790697‑3791130                                                                                                                                                                                                                             |                                                                                                                                                                                                                      tagCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGAtttt                                                                                                                                                                                          <  1:332170/248‑1 (MQ=255)           gaCCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGa                                                                                                                                                                               <  1:286917/250‑1 (MQ=255)            aCCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCCCCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGaa                                                                                                                                                                              <  1:72950/251‑1 (MQ=255)                 aCGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCg                                                                                                                                                                         >  1:237303/1‑251 (MQ=255)                 aCGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCg                                                                                                                                                                         >  1:316260/1‑251 (MQ=255)                   gggTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCggg                                                                                                                                                                       >  1:329362/1‑251 (MQ=255)                     gggacaCCCATTTTGCGCGCCAGTTCGATACGATATTCATTAACATCAGGGACCACCACATTCCGTGCCCCAACGGGTTCCGCCTCCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGAt                                                                                                                                                                     <  2:309999/246‑1 (MQ=255)                                  tgcgcgCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCg                                                                                                                                                        >  1:290646/3‑251 (MQ=255)                                  tgcccgccagttcgagacgttATTCATTAACATCAGTGATCACCCCATTGCGTGCCCCAACGTGTTTCGCCCCCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTc                                                                                                                                                         <  2:147274/230‑1 (MQ=255)                                      cgccagttcgagacgatATTCATTAACATCCGTGCTCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGTATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGcg                                                                                                                                                     >  1:141177/17‑249 (MQ=255)                                              cgagacgatATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGccc                                                                                                                                            >  1:96581/9‑251 (MQ=255)                                              cgagacgatATTCATTAACATCAGGGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGcc                                                                                                                                             <  1:272148/242‑1 (MQ=255)                                                          cattAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAacg                                                                                                                                >  2:318795/3‑250 (MQ=255)                                                              accaacAGTGATCACCACATTGCGTCCGTCATCGTGTTTCGCCTCCGCCGCAGCCATAATACCTATCGGATCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAacgccga                                                                                                                            <  1:151002/246‑6 (MQ=255)                                                                    aGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCACGGACGGTTAACGCCGACGccg                                                                                                                       >  1:332659/1‑249 (MQ=255)                                                                     gTGATCACCACAGTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGccgat                                                                                                                     <  1:396238/251‑4 (MQ=255)                                                                        aTCACCACATTGCGTGCCCCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCTGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCgt                                                                                                                  <  1:74472/251‑1 (MQ=255)                                                                        aTCACCACATTGCGTGCACCCAAGTGATTCGCAACCGCCGCAGCAATCATACCTACCGGACCTGCACCAGAGCCCAGCACATCCTCGCCACCCAGCTCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGCTCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCgt                                                                                                                  >  2:383074/1‑251 (MQ=255)                                                                                         ccaaaCGGGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATggg                                                                                                 <  2:187316/248‑1 (MQ=255)                                                                                         aCCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATc                                                                                                                    <  1:45921/232‑1 (MQ=255)                                                                                         aCCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATc                                                                                                                    >  2:45921/1‑232 (MQ=255)                                                                                             aCGTGTTCCCCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACg                                                                                             <  2:96581/251‑1 (MQ=255)                                                                                               gtgtTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAagca                                                                                                                                                <  1:79029/198‑1 (MQ=255)                                                                                               gtgtTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAagca                                                                                                                                                >  2:79029/1‑198 (MQ=255)                                                                                               gtgtTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACGac                                                                                           >  2:121527/1‑251 (MQ=255)                                                                                               gtgtTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACGac                                                                                           >  1:226537/1‑251 (MQ=255)                                                                                                      gCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGGACGACCGCCACg                                                                                    >  1:73838/1‑251 (MQ=255)                                                                                                                           cctaTCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGCAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGAccgc                                                                >  1:418146/4‑248 (MQ=255)                                                                                                                                 ggaccTGCACCAGTGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCgacagggaccgcaggggat                                                         >  2:138650/4‑233 (MQ=255)                                                                                                                                  gaccTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGat                                                         >  1:399815/3‑250 (MQ=255)                                                                                                                                        cACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCt                                                  >  2:10120/1‑251 (MQ=255)                                                                                                                                                       caTCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGCAGGGATCAAAAATGGACGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGAcgcg                                    >  2:162685/1‑250 (MQ=255)                                                                                                                                                          cctCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCg                                >  1:44232/3‑251 (MQ=255)                                                                                                                                                                caaccaGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATc                          >  1:188864/3‑251 (MQ=255)                                                                                                                                                                               aCAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGATCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACt           >  2:126116/1‑251 (MQ=255)                                                                                                                                                                                         aTGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGATTTTGAAGGCGTTGAACGCCGGGATCACCAGATATTCGGCGAAGCAGCCCGGACGGTTAACGCCGACGCCGCTCGTGTTGCGGCACAGATGGGTACGACCGCCACGACAGTTGCGACAGTGACCGCAGGTGATATGGCCTTCTCCAGAGACGCGATCGCCAATCTTAAAGCCTTTCACTTCCTGAccg  >  2:161221/1‑249 (MQ=255)                                                                                                                                                                                                                             |                                                                                                                                                                                                                      TGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTTGAAGGCGTTGAATGCCGGGATCACCAGATATTCGGCAAAGCAGCCCGGGCGATTAACACCAACGCCTATCGTGTTGCGGCACAAATGGGTACGACCACCACGACAGTTGCGGCAATGACCACAGGTGATATGGCCTTCGCCAGAAACGCGATCGCCGATCTTGAAGCCTTTCACTTCCTGACCA  >  NC_000913/3790697‑3791130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑