Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,790,707	A→G	V213V (GTT→GTC)	tdh ←	threonine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,790,707	0	A	G	100.0%	97.0 / NA	33	V213V (GTT→GTC)	tdh	threonine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/12);  total (21/12)

ACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAACCTTCGGTCATGCCTAACTCCGCCATCACGTCATTGAGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTT  >  NC_000913/3790482‑3790946                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                aCCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGAGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCAttttgc                                                                                                                                                                                                                        >  2:286917/1‑249 (MQ=255)                                  gATCAATTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGAGCCAGTTCGAGACGATATTCATTAACATc                                                                                                                                                                                         >  1:349989/1‑249 (MQ=255)                                                     atcgACATGTCAGAAGGTGGAATACCCAGCACCGCAAAACGACCGCCGTGGTTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATCAACATCAGTGATCACCACATTGCGTGc                                                                                                                                                                    <  2:46970/248‑1 (MQ=255)                                                     acctacatgtcagaaggtgaaaTACCCATCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCTGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTGTAGCGACGTTGACCGCACGGTTGATACCCATTTTGCGCGCCAGTTCGATACGATATTGATTAACATCAGTGACCACCACATTGCGTg                                                                                                                                                                     <  2:176895/230‑1 (MQ=255)                                                               cagaaggtggAATACCCAGCATCGCAAGACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACgtgt                                                                                                                                                          <  2:105684/243‑1 (MQ=255)                                                                agaaggtggAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCAAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGtt                                                                                                                                                         >  1:38322/8‑251 (MQ=255)                                                                 gaaggatgAATACCCAGCATGGCAATAAGACAGCCGAGGTTCAAGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGCCATCAAAGCCTTCGGTCATACCCAGTTCTGCCATCACATCATTCAGGTTTTCTTTGGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGCTGTTCATTACCATCAGTGATCACCACCTTGCGTGCACCAACGTGttt                                                                                                                                                        >  2:357755/8‑251 (MQ=255)                                                                         aaaccccAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCAccg                                                                                                                                                <  2:300776/247‑1 (MQ=255)                                                                          aTACCCCGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGCGCATGGTACGAAACGCTGGCGGCGCCCCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCCCGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGGGTTTCGCCAccgc                                                                                                                                               >  2:50342/1‑251 (MQ=255)                                                                            aCCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATAACCACATTGCGTGCACCAACGTGTTTCGCCAccgccg                                                                                                                                             >  1:249111/1‑251 (MQ=255)                                                                             cccAGCATCCCAATACGCCCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCGGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTGCGCCACcgccgc                                                                                                                                            <  2:75230/251‑1 (MQ=255)                                                                               cAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGCTACCCATTTTGCGCGCCAgttcgagac                                                                                                                                                                                                         >  1:297895/1‑180 (MQ=255)                                                                               cAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAgttcgagac                                                                                                                                                                                                         <  2:297895/188‑9 (MQ=255)                                                                                  cATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCgccgccgcc                                                                                                                                        >  1:436395/1‑250 (MQ=255)                                                                                            cgaccgccgTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATAcctat                                                                                                                             >  1:184925/4‑248 (MQ=255)                                                                                                 gccgTGGTTCATGGTGTCGAGAATGGTACGAAACGCTGGCGCCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAAAGTGTTTCGCCACCGCCGCAGCCATCATACCTATCggac                                                                                                                        >  2:227453/1‑249 (MQ=255)                                                                                                        ttCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCAcc                                                                                                                 >  2:272148/1‑251 (MQ=255)                                                                                                                gtcgaGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAATTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGAcc                                                                                                          >  1:294377/5‑250 (MQ=255)                                                                                                                              gAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGcc                                                                                            >  1:409294/1‑250 (MQ=255)                                                                                                                                        cggcgCCCCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATcga                                                                                 <  1:323839/251‑3 (MQ=255)                                                                                                                                        cggcgCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATcg                                                                                  >  2:74472/1‑249 (MQ=255)                                                                                                                                                         ccAGACCGACATCAAAGCCTTCGGTCATGCCCTGTTCTGCCATCACATCAGTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCATTGCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGc                                                                <  1:308876/251‑1 (MQ=255)                                                                                                                                                                      aaagccTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCg                                                    >  1:342062/5‑250 (MQ=255)                                                                                                                                                                         gccTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAgg                                                >  1:221625/2‑251 (MQ=255)                                                                                                                                                                         cccTTCGGTCTTGCCCATTTCTGCCATCACATCATTAAGGTTTTCTTGAGCGACGTGGACCGCCCGGGTGATACCCATTTGGCGCGCCAGTTCGAGACGATATTCATTAACACCAGTGATCACCACATTGCGTGCCCCACCGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAgg                                                <  1:240831/250‑1 (MQ=255)                                                                                                                                                                             tCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGCTCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCCTAATACCTATCGGACCTGCCCCAGAGCCCCGCACATCCTCGCCAACCAGATCGACCGGCAGCAAGGTATGCACGGCGTTACCGAACGGATc                                            >  1:177353/1‑251 (MQ=255)                                                                                                                                                                                    tgcccagttctgCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAAtg                                     >  2:72950/12‑250 (MQ=255)                                                                                                                                                                                     gcccagttctgCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCTTTTTGCGCGCCATTTCGAGACGATATTCATTAACCTCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAAtgg                                    <  1:37800/241‑3 (MQ=255)                                                                                                                                                                                       ccagttctgCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAAtggaa                                  <  1:23713/243‑5 (MQ=255)                                                                                                                                                                                                 catcacatcaTTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAAtggaagccaggtcgt                        >  2:396238/1‑237 (MQ=255)                                                                                                                                                                                                  aTCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGAGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTACCATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCAGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGACCGACAGCGCGGTATGCACGGCGTTACCGAAGGGGTCAAAAAtggaagccaggtcgtc                       >  1:438507/1‑236 (MQ=255)                                                                                                                                                                                                       atCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAAtggaagccaggtcgtcgg                     >  1:353733/2‑231 (MQ=255)                                                                                                                                                                                                                        tagCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATGGAAGCCAGGTCGTCGGAGATATTGTCTGGGAtttt  <  1:332170/248‑1 (MQ=255)                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                ACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAACCTTCGGTCATGCCTAACTCCGCCATCACGTCATTGAGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATCGGAAATATTGTCGGGGATTTT  >  NC_000913/3790482‑3790946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑