Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,790,680	G→A	D222D (GAC→GAT)	tdh ←	threonine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,790,680	0	G	A	97.1%	108.6 / NA	34	D222D (GAC→GAT)	tdh	threonine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (21/12);  minor base C (1/0);  total (22/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAACCTTCGGTCATGCCTAACTCCGCCATCACGTCATTGAGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATC  >  NC_000913/3790441‑3790925                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                      aTCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCTTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCAttaaggtt                                                                                                                                                                                                                                            <  1:385791/251‑7 (MQ=255)   cAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGtttt                                                                                                                                                                                                                                          >  1:309999/1‑251 (MQ=255)        ccgccATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACCTCATTAAGGTTTTCttt                                                                                                                                                                                                                                      >  1:297353/1‑250 (MQ=255)           ccATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCg                                                                                                                                                                                                                                  >  1:75230/1‑251 (MQ=255)                                          aCCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGAGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCAttttgc                                                                                                                                                                                                   >  2:286917/1‑249 (MQ=255)                                                                           gATCAATTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGAGCCAGTTCGAGACGATATTCATTAACATc                                                                                                                                                                    >  1:349989/1‑249 (MQ=255)                                                                                              atcgACATGTCAGAAGGTGGAATACCCAGCACCGCAAAACGACCGCCGTGGTTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATCAACATCAGTGATCACCACATTGCGTGc                                                                                                                                               <  2:46970/248‑1 (MQ=255)                                                                                              acctacatgtcagaaggtgaaaTACCCATCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCTGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTGTAGCGACGTTGACCGCACGGTTGATACCCATTTTGCGCGCCAGTTCGATACGATATTGATTAACATCAGTGACCACCACATTGCGTg                                                                                                                                                <  2:176895/230‑1 (MQ=255)                                                                                                        cagaaggtggAATACCCAGCATCGCAAGACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACgtgt                                                                                                                                     <  2:105684/243‑1 (MQ=255)                                                                                                         agaaggtggAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCAAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGtt                                                                                                                                    >  1:38322/8‑251 (MQ=255)                                                                                                          gaaggatgAATACCCAGCATGGCAATAAGACAGCCGAGGTTCAAGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGCCATCAAAGCCTTCGGTCATACCCAGTTCTGCCATCACATCATTCAGGTTTTCTTTGGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGCTGTTCATTACCATCAGTGATCACCACCTTGCGTGCACCAACGTGttt                                                                                                                                   >  2:357755/8‑251 (MQ=255)                                                                                                                  aaaccccAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCAccg                                                                                                                           <  2:300776/247‑1 (MQ=255)                                                                                                                   aTACCCCGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGCGCATGGTACGAAACGCTGGCGGCGCCCCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCCCGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGGGTTTCGCCAccgc                                                                                                                          >  2:50342/1‑251 (MQ=255)                                                                                                                     aCCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATAACCACATTGCGTGCACCAACGTGTTTCGCCAccgccg                                                                                                                        >  1:249111/1‑251 (MQ=255)                                                                                                                      cccAGCATCCCAATACGCCCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCGGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTGCGCCACcgccgc                                                                                                                       <  2:75230/251‑1 (MQ=255)                                                                                                                        cAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGCTACCCATTTTGCGCGCCAgttcgagac                                                                                                                                                                                    >  1:297895/1‑180 (MQ=255)                                                                                                                        cAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAgttcgagac                                                                                                                                                                                    <  2:297895/188‑9 (MQ=255)                                                                                                                           cATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCgccgccgcc                                                                                                                   >  1:436395/1‑250 (MQ=255)                                                                                                                                     cgaccgccgTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATAcctat                                                                                                        >  1:184925/4‑248 (MQ=255)                                                                                                                                          gccgTGGTTCATGGTGTCGAGAATGGTACGAAACGCTGGCGCCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAAAGTGTTTCGCCACCGCCGCAGCCATCATACCTATCggac                                                                                                   >  2:227453/1‑249 (MQ=255)                                                                                                                                                 ttCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCAcc                                                                                            >  2:272148/1‑251 (MQ=255)                                                                                                                                                         gtcgaGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAATTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGAcc                                                                                     >  1:294377/5‑250 (MQ=255)                                                                                                                                                                       gAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGcc                                                                       >  1:409294/1‑250 (MQ=255)                                                                                                                                                                                 cggcgCCCCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATcga                                                            <  1:323839/251‑3 (MQ=255)                                                                                                                                                                                 cggcgCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATcg                                                             >  2:74472/1‑249 (MQ=255)                                                                                                                                                                                                  ccAGACCGACATCAAAGCCTTCGGTCATGCCCTGTTCTGCCATCACATCAGTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCATTGCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGc                                           <  1:308876/251‑1 (MQ=255)                                                                                                                                                                                                               aaagccTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCg                               >  1:342062/5‑250 (MQ=255)                                                                                                                                                                                                                  gccTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAgg                           >  1:221625/2‑251 (MQ=255)                                                                                                                                                                                                                  cccTTCGGTCTTGCCCATTTCTGCCATCACATCATTAAGGTTTTCTTGAGCGACGTGGACCGCCCGGGTGATACCCATTTGGCGCGCCAGTTCGAGACGATATTCATTAACACCAGTGATCACCACATTGCGTGCCCCACCGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAgg                           <  1:240831/250‑1 (MQ=255)                                                                                                                                                                                                                      tCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGCTCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCCTAATACCTATCGGACCTGCCCCAGAGCCCCGCACATCCTCGCCAACCAGATCGACCGGCAGCAAGGTATGCACGGCGTTACCGAACGGATc                       >  1:177353/1‑251 (MQ=255)                                                                                                                                                                                                                             tgcccagttctgCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAAtg                >  2:72950/12‑250 (MQ=255)                                                                                                                                                                                                                              gcccagttctgCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCTTTTTGCGCGCCATTTCGAGACGATATTCATTAACCTCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAAtgg               <  1:37800/241‑3 (MQ=255)                                                                                                                                                                                                                                ccagttctgCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAAtggaa             <  1:23713/243‑5 (MQ=255)                                                                                                                                                                                                                                          catcacatcaTTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAAtggaagccaggtcgt   >  2:396238/1‑237 (MQ=255)                                                                                                                                                                                                                                           aTCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGAGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTACCATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCAGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGACCGACAGCGCGGTATGCACGGCGTTACCGAAGGGGTCAAAAAtggaagccaggtcgtc  >  1:438507/1‑236 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                      ATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAACCTTCGGTCATGCCTAACTCCGCCATCACGTCATTGAGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATCAAAAATTGCGGCTAAGTCATC  >  NC_000913/3790441‑3790925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑