Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,790,671	C→T	A225A (GCG→GCA)	tdh ←	threonine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,790,671	0	C	T	100.0%	98.6 / NA	30	A225A (GCG→GCA)	tdh	threonine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/10);  total (20/10)

ATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAACCTTCGGTCATGCCTAACTCCGCCATCACGTCATTGAGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATC  >  NC_000913/3790441‑3790904                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                          aTCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCTTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCAttaaggtt                                                                                                                                                                                                                       <  1:385791/251‑7 (MQ=255)   cAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGtttt                                                                                                                                                                                                                     >  1:309999/1‑251 (MQ=255)        ccgccATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACCTCATTAAGGTTTTCttt                                                                                                                                                                                                                 >  1:297353/1‑250 (MQ=255)           ccATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCg                                                                                                                                                                                                             >  1:75230/1‑251 (MQ=255)                                          aCCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGAGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCAttttgc                                                                                                                                                                              >  2:286917/1‑249 (MQ=255)                                                                           gATCAATTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGAGCCAGTTCGAGACGATATTCATTAACATc                                                                                                                                               >  1:349989/1‑249 (MQ=255)                                                                                              atcgACATGTCAGAAGGTGGAATACCCAGCACCGCAAAACGACCGCCGTGGTTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATCAACATCAGTGATCACCACATTGCGTGc                                                                                                                          <  2:46970/248‑1 (MQ=255)                                                                                              acctacatgtcagaaggtgaaaTACCCATCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCTGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTGTAGCGACGTTGACCGCACGGTTGATACCCATTTTGCGCGCCAGTTCGATACGATATTGATTAACATCAGTGACCACCACATTGCGTg                                                                                                                           <  2:176895/230‑1 (MQ=255)                                                                                                        cagaaggtggAATACCCAGCATCGCAAGACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACgtgt                                                                                                                <  2:105684/243‑1 (MQ=255)                                                                                                         agaaggtggAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCAAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGtt                                                                                                               >  1:38322/8‑251 (MQ=255)                                                                                                          gaaggatgAATACCCAGCATGGCAATAAGACAGCCGAGGTTCAAGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGCCATCAAAGCCTTCGGTCATACCCAGTTCTGCCATCACATCATTCAGGTTTTCTTTGGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGCTGTTCATTACCATCAGTGATCACCACCTTGCGTGCACCAACGTGttt                                                                                                              >  2:357755/8‑251 (MQ=255)                                                                                                                  aaaccccAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCAccg                                                                                                      <  2:300776/247‑1 (MQ=255)                                                                                                                   aTACCCCGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGCGCATGGTACGAAACGCTGGCGGCGCCCCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCCCGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGGGTTTCGCCAccgc                                                                                                     >  2:50342/1‑251 (MQ=255)                                                                                                                     aCCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATAACCACATTGCGTGCACCAACGTGTTTCGCCAccgccg                                                                                                   >  1:249111/1‑251 (MQ=255)                                                                                                                      cccAGCATCCCAATACGCCCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCGGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTGCGCCACcgccgc                                                                                                  <  2:75230/251‑1 (MQ=255)                                                                                                                        cAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGCTACCCATTTTGCGCGCCAgttcgagac                                                                                                                                                               >  1:297895/1‑180 (MQ=255)                                                                                                                        cAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAgttcgagac                                                                                                                                                               <  2:297895/188‑9 (MQ=255)                                                                                                                           cATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCgccgccgcc                                                                                              >  1:436395/1‑250 (MQ=255)                                                                                                                                     cgaccgccgTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATAcctat                                                                                   >  1:184925/4‑248 (MQ=255)                                                                                                                                          gccgTGGTTCATGGTGTCGAGAATGGTACGAAACGCTGGCGCCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAAAGTGTTTCGCCACCGCCGCAGCCATCATACCTATCggac                                                                              >  2:227453/1‑249 (MQ=255)                                                                                                                                                 ttCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCAcc                                                                       >  2:272148/1‑251 (MQ=255)                                                                                                                                                         gtcgaGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAATTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGAcc                                                                >  1:294377/5‑250 (MQ=255)                                                                                                                                                                       gAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGcc                                                  >  1:409294/1‑250 (MQ=255)                                                                                                                                                                                 cggcgCCCCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATcga                                       <  1:323839/251‑3 (MQ=255)                                                                                                                                                                                 cggcgCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATcg                                        >  2:74472/1‑249 (MQ=255)                                                                                                                                                                                                  ccAGACCGACATCAAAGCCTTCGGTCATGCCCTGTTCTGCCATCACATCAGTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCATTGCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGc                      <  1:308876/251‑1 (MQ=255)                                                                                                                                                                                                               aaagccTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCg          >  1:342062/5‑250 (MQ=255)                                                                                                                                                                                                                  gccTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAgg      >  1:221625/2‑251 (MQ=255)                                                                                                                                                                                                                  cccTTCGGTCTTGCCCATTTCTGCCATCACATCATTAAGGTTTTCTTGAGCGACGTGGACCGCCCGGGTGATACCCATTTGGCGCGCCAGTTCGAGACGATATTCATTAACACCAGTGATCACCACATTGCGTGCCCCACCGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCACCAGAGACCAGCACATCCTCGCCAACCAGATCGAACGACAGCGCGGTATGCACGGCGTTACCGAAgg      <  1:240831/250‑1 (MQ=255)                                                                                                                                                                                                                      tCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGCTCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCCTAATACCTATCGGACCTGCCCCAGAGCCCCGCACATCCTCGCCAACCAGATCGACCGGCAGCAAGGTATGCACGGCGTTACCGAACGGATc  >  1:177353/1‑251 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                          ATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAACCTTCGGTCATGCCTAACTCCGCCATCACGTCATTGAGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACCAGAAACCAGCACATCTTCGCCCACCAGATCAAACGACAGCGCGGTATGCACGGCGTTACCGAAGGGATC  >  NC_000913/3790441‑3790904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑