| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,790,596 | A→G | L250L (CTT→CTC) | tdh ← | threonine dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,790,596 | 0 | A | G | 94.3% | 104.0 / ‑1.5 | 35 | L250L (CTT→CTC) | tdh | threonine dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (21/12); total (22/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.07e-01 | |||||||||||
GGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAACCTTCGGTCATGCCTAACTCCGCCATCACGTCATTGAGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACC > NC_000913/3790352‑3790835 | ggCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTCTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCAAGCATg > 1:46970/1‑251 (MQ=255) gCGTCAAAGCCCTTCGGGAAAGCATCGATAGAGAACCGATGAGAAATGAGCGGCGAGAGATCGAGGCCAGACTGAATCAGCCCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGACCACTTTTGTCCAGGCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTgg < 2:433852/251‑1 (MQ=255) cGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATCCCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTggc < 1:97667/251‑1 (MQ=255) cccTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCAcc > 1:187316/1‑251 (MQ=255) catcGATAGAGACCCGATGAGTACTGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCAATCTTGTACCCGGTTTCAAACATCTCACGCCCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAgc > 2:37800/1‑250 (MQ=255) gaaccGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAACCATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGCGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGGGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACAtac > 2:240831/5‑248 (MQ=255) gagTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTg < 1:112119/208‑1 (MQ=255) gagTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTg > 2:112119/3‑210 (MQ=255) gaGATCGAGGCCAGACTGAATCAGCGCCGCCATCTGGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGcccagttctg < 2:29469/250‑9 (MQ=255) ccAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGTCCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGcccagttcggccatcacatc < 1:363403/251‑19 (MQ=255) ccAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGcccagttctgccatcacatc > 1:105684/1‑233 (MQ=255) cAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGcccagttctgccatcacatc > 1:300776/1‑232 (MQ=255) aTCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCTTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCAttaaggtt < 1:385791/251‑7 (MQ=255) cAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGtttt > 1:309999/1‑251 (MQ=255) ccgccATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACCTCATTAAGGTTTTCttt > 1:297353/1‑250 (MQ=255) ccATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCg > 1:75230/1‑251 (MQ=255) aCCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGAGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCAttttgc > 2:286917/1‑249 (MQ=255) cttaaaTGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCATAAGGGGGAAGACCCAGCATCGCAATACGACCGCCGTTGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCg < 2:174562/145‑1 (MQ=255) cTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCg > 1:174562/1‑149 (MQ=255) gATCAATTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGAGCCAGTTCGAGACGATATTCATTAACATc > 1:349989/1‑249 (MQ=255) atcgACATGTCAGAAGGTGGAATACCCAGCACCGCAAAACGACCGCCGTGGTTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATCAACATCAGTGATCACCACATTGCGTGc < 2:46970/248‑1 (MQ=255) acctacatgtcagaaggtgaaaTACCCATCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCTGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTGTAGCGACGTTGACCGCACGGTTGATACCCATTTTGCGCGCCAGTTCGATACGATATTGATTAACATCAGTGACCACCACATTGCGTg < 2:176895/230‑1 (MQ=255) cagaaggtggAATACCCAGCATCGCAAGACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACgtgt < 2:105684/243‑1 (MQ=255) agaaggtggAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCAAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGtt > 1:38322/8‑251 (MQ=255) gaaggatgAATACCCAGCATGGCAATAAGACAGCCGAGGTTCAAGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGCCATCAAAGCCTTCGGTCATACCCAGTTCTGCCATCACATCATTCAGGTTTTCTTTGGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGCTGTTCATTACCATCAGTGATCACCACCTTGCGTGCACCAACGTGttt > 2:357755/8‑251 (MQ=255) aaaccccAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCAccg < 2:300776/247‑1 (MQ=255) aTACCCCGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGCGCATGGTACGAAACGCTGGCGGCGCCCCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCCCGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGGGTTTCGCCAccgc > 2:50342/1‑251 (MQ=255) aCCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATAACCACATTGCGTGCACCAACGTGTTTCGCCAccgccg > 1:249111/1‑251 (MQ=255) cccAGCATCCCAATACGCCCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCGGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTGCGCCACcgccgc < 2:75230/251‑1 (MQ=255) cAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGCTACCCATTTTGCGCGCCAgttcgagac > 1:297895/1‑180 (MQ=255) cAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAgttcgagac < 2:297895/188‑9 (MQ=255) cATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCgccgccgcc > 1:436395/1‑250 (MQ=255) cgaccgccgTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATAcctat > 1:184925/4‑248 (MQ=255) gccgTGGTTCATGGTGTCGAGAATGGTACGAAACGCTGGCGCCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAAAGTGTTTCGCCACCGCCGCAGCCATCATACCTATCggac > 2:227453/1‑249 (MQ=255) ttCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGCGCCAGTTCGAGACGATATTCATTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCAGCCATAATACCTATCGGACCTGCAcc > 2:272148/1‑251 (MQ=255) | GGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAACCTTCGGTCATGCCTAACTCCGCCATCACGTCATTGAGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATCAGTGATCACCACATTGCGTGCACCAACGTGTTTCGCCACCGCCGCTGCCATAATACCAATCGGGCCTGCACC > NC_000913/3790352‑3790835 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |