Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,790,533	G→T	I271I (ATC→ATA)	tdh ←	threonine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,790,533	0	G	T	100.0%	101.1 / NA	31	I271I (ATC→ATA)	tdh	threonine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (21/10);  total (21/10)

ATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAACCTTCGGTCATGCCTAACTCCGCCATCACGTCATTGAGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATC  >  NC_000913/3790301‑3790763                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                       aTACCAGCCCTTGTTAGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACAGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGaaggg                                                                                                                                                                                                                      >  2:323839/1‑247 (MQ=255)   aCCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGCCCGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGaaggtgg                                                                                                                                                                                                                    >  2:23713/1‑245 (MQ=255)                cGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGCATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATc                                                                                                                                                                                                       >  1:51285/1‑251 (MQ=255)                                            cccGGACTGGCCCGAACGCATAGCTTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACAGCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAGGGTGAAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGgtgt                                                                                                                                                                           <  2:289032/251‑1 (MQ=255)                                            cccGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGCTCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGGGGTTCATGgtgt                                                                                                                                                                           >  2:332170/1‑251 (MQ=255)                                                gACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTcg                                                                                                                                                                         >  1:181386/1‑248 (MQ=255)                                                    ggCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTCTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCAAGCATg                                                                                                                                                                   >  1:46970/1‑251 (MQ=255)                                                                  gCGTCAAAGCCCTTCGGGAAAGCATCGATAGAGAACCGATGAGAAATGAGCGGCGAGAGATCGAGGCCAGACTGAATCAGCCCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGACCACTTTTGTCCAGGCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTgg                                                                                                                                                     <  2:433852/251‑1 (MQ=255)                                                                   cGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATCCCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTggc                                                                                                                                                    <  1:97667/251‑1 (MQ=255)                                                                     tCAAAGCCCTTCTGGACATCATCGATAGAGCACCGATGAGTAATGATCGGCGTGAGATCGAGGCCAGACTGAATCAGCGCCGCCCTCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATAc                                                                                                                                                                                                               >  1:376517/1‑190 (MQ=255)                                                                     tCAAAGCCCGTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCGTTAATGAACAAGCCCGTAAAGATCACTTTTGTCCAGTCTATCGACATGGCAGAAGGTGGAATAc                                                                                                                                                                                                               <  2:376517/190‑1 (MQ=255)                                                                           cccTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCAcc                                                                                                                                            >  1:187316/1‑251 (MQ=255)                                                                                        catcGATAGAGACCCGATGAGTACTGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCAATCTTGTACCCGGTTTCAAACATCTCACGCCCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAgc                                                                                                                               >  2:37800/1‑250 (MQ=255)                                                                                                  gaaccGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAACCATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGCGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGGGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACAtac                                                                                                                      >  2:240831/5‑248 (MQ=255)                                                                                                          gagTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTg                                                                                                                                                      <  1:112119/208‑1 (MQ=255)                                                                                                          gagTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTg                                                                                                                                                      >  2:112119/3‑210 (MQ=255)                                                                                                                          gaGATCGAGGCCAGACTGAATCAGCGCCGCCATCTGGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGcccagttctg                                                                                             <  2:29469/250‑9 (MQ=255)                                                                                                                                    ccAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGTCCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGcccagttcggccatcacatc                                                                                   <  1:363403/251‑19 (MQ=255)                                                                                                                                    ccAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGcccagttctgccatcacatc                                                                                   >  1:105684/1‑233 (MQ=255)                                                                                                                                     cAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGcccagttctgccatcacatc                                                                                   >  1:300776/1‑232 (MQ=255)                                                                                                                                           gAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTcgag                                                                                                                                                                       >  1:177046/1‑157 (MQ=255)                                                                                                                                           gAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCGCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGGCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTcgag                                                                                                                                                                       <  2:177046/160‑4 (MQ=255)                                                                                                                                             aTCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCTTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCAttaaggtt                                                                          <  1:385791/251‑7 (MQ=255)                                                                                                                                               cAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGtttt                                                                        >  1:309999/1‑251 (MQ=255)                                                                                                                                                aGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATgg                                                                                                                                                                              >  1:300044/1‑148 (MQ=255)                                                                                                                                                    ccgccATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACCTCATTAAGGTTTTCttt                                                                    >  1:297353/1‑250 (MQ=255)                                                                                                                                                       ccATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCg                                                                >  1:75230/1‑251 (MQ=255)                                                                                                                                                                                      aCCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGAGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCAttttgc                                 >  2:286917/1‑249 (MQ=255)                                                                                                                                                                                                 cttaaaTGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCATAAGGGGGAAGACCCAGCATCGCAATACGACCGCCGTTGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCg                                                                                                                            <  2:174562/145‑1 (MQ=255)                                                                                                                                                                                                 cTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCg                                                                                                                            >  1:174562/1‑149 (MQ=255)                                                                                                                                                                                                                       gATCAATTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCATTTTGCGAGCCAGTTCGAGACGATATTCATTAACATc  >  1:349989/1‑249 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                       ATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAACCTTCGGTCATGCCTAACTCCGCCATCACGTCATTGAGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTACGCGCCAGCTCAAGGCGGTATTCGTTAACATC  >  NC_000913/3790301‑3790763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑