Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,790,509	T→C	K279K (AAA→AAG)	tdh ←	threonine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,790,509	0	T	C	100.0%	126.7 / NA	37	K279K (AAA→AAG)	tdh	threonine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/12);  total (25/12)

TTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAACCTTCGGTCATGCCTAACTCCGCCATCACGTCATTGAGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTAC  >  NC_000913/3790269‑3790732                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                ttAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCAGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGCGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATc                                                                                                                                                                                                                        >  1:176895/1‑250 (MQ=255)          tATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTcc                                                                                                                                                                                                              >  2:97667/1‑251 (MQ=255)                  gATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTctatc                                                                                                                                                                                                      >  1:29469/1‑247 (MQ=255)                                 aTACCAGCCCTTGTTAGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACAGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGaaggg                                                                                                                                                                                       >  2:323839/1‑247 (MQ=255)                                   aCCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGCCCGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGaaggtgg                                                                                                                                                                                     >  2:23713/1‑245 (MQ=255)                                                cGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGCATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATc                                                                                                                                                                        >  1:51285/1‑251 (MQ=255)                                                                            cccGGACTGGCCCGAACGCATAGCTTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACAGCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAGGGTGAAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGgtgt                                                                                                                                            <  2:289032/251‑1 (MQ=255)                                                                            cccGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGCTCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGGGGTTCATGgtgt                                                                                                                                            >  2:332170/1‑251 (MQ=255)                                                                                gACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTcg                                                                                                                                          >  1:181386/1‑248 (MQ=255)                                                                                    ggCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTCTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCAAGCATg                                                                                                                                    >  1:46970/1‑251 (MQ=255)                                                                                           aCGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTcc                                                                                                                                                                                                              <  1:187740/170‑1 (MQ=255)                                                                                           aCGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGCAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTcc                                                                                                                                                                                                              >  2:187740/1‑170 (MQ=255)                                                                                               aTAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCAc                                                                                                                                                                                                                      <  2:83038/158‑1 (MQ=255)                                                                                               aTAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCAc                                                                                                                                                                                                                      >  1:83038/1‑158 (MQ=255)                                                                                                  gCGTCAAAGCCCTTCGGGAAAGCATCGATAGAGAACCGATGAGAAATGAGCGGCGAGAGATCGAGGCCAGACTGAATCAGCCCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGACCACTTTTGTCCAGGCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTgg                                                                                                                      <  2:433852/251‑1 (MQ=255)                                                                                                   cGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATCCCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTggc                                                                                                                     <  1:97667/251‑1 (MQ=255)                                                                                                     tCAAAGCCCTTCTGGACATCATCGATAGAGCACCGATGAGTAATGATCGGCGTGAGATCGAGGCCAGACTGAATCAGCGCCGCCCTCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATAc                                                                                                                                                                                >  1:376517/1‑190 (MQ=255)                                                                                                     tCAAAGCCCGTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCGTTAATGAACAAGCCCGTAAAGATCACTTTTGTCCAGTCTATCGACATGGCAGAAGGTGGAATAc                                                                                                                                                                                <  2:376517/190‑1 (MQ=255)                                                                                                           cccTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCAcc                                                                                                             >  1:187316/1‑251 (MQ=255)                                                                                                                        catcGATAGAGACCCGATGAGTACTGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCAATCTTGTACCCGGTTTCAAACATCTCACGCCCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAgc                                                                                                >  2:37800/1‑250 (MQ=255)                                                                                                                                  gaaccGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAACCATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGCGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGGGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACAtac                                                                                       >  2:240831/5‑248 (MQ=255)                                                                                                                                          gagTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTg                                                                                                                       <  1:112119/208‑1 (MQ=255)                                                                                                                                          gagTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTg                                                                                                                       >  2:112119/3‑210 (MQ=255)                                                                                                                                                          gaGATCGAGGCCAGACTGAATCAGCGCCGCCATCTGGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGcccagttctg                                                              <  2:29469/250‑9 (MQ=255)                                                                                                                                                                    ccAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGTCCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGcccagttcggccatcacatc                                                    <  1:363403/251‑19 (MQ=255)                                                                                                                                                                    ccAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGcccagttctgccatcacatc                                                    >  1:105684/1‑233 (MQ=255)                                                                                                                                                                     cAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGcccagttctgccatcacatc                                                    >  1:300776/1‑232 (MQ=255)                                                                                                                                                                           gAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTcgag                                                                                                                                        >  1:177046/1‑157 (MQ=255)                                                                                                                                                                           gAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCGCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGGCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTcgag                                                                                                                                        <  2:177046/160‑4 (MQ=255)                                                                                                                                                                             aTCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCTTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCAttaaggtt                                           <  1:385791/251‑7 (MQ=255)                                                                                                                                                                               cAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGtttt                                         >  1:309999/1‑251 (MQ=255)                                                                                                                                                                                aGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATgg                                                                                                                                               >  1:300044/1‑148 (MQ=255)                                                                                                                                                                                    ccgccATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACCTCATTAAGGTTTTCttt                                     >  1:297353/1‑250 (MQ=255)                                                                                                                                                                                       ccATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCg                                 >  1:75230/1‑251 (MQ=255)                                                                                                                                                                                                                      aCCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGAGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAGCCTTCGGTCATGCCCAGTTCTGCCATCACATCATTAAGGTTTTCTTTAGCGACGTTGACCGCACGGGTGATACCCAttttgc  >  2:286917/1‑249 (MQ=255)                                                                                                                                                                                                                                 cttaaaTGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCATAAGGGGGAAGACCCAGCATCGCAATACGACCGCCGTTGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCg                                                                                             <  2:174562/145‑1 (MQ=255)                                                                                                                                                                                                                                 cTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCg                                                                                             >  1:174562/1‑149 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                TTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCAAAACCTTCGGTCATGCCTAACTCCGCCATCACGTCATTGAGATTTTCTTTGGCGACGTTAACCGCACGGGTGATACCCATTTTAC  >  NC_000913/3790269‑3790732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑