Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,790,407	G→A	T313T (ACC→ACT)	tdh ←	threonine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,790,407	0	G	A	97.0%	95.5 / NA	33	T313T (ACC→ACT)	tdh	threonine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (21/11);  minor base C (1/0);  total (22/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCA  >  NC_000913/3790177‑3790647                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 agTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATc                                                                                                                                                                                                                              <  1:66505/251‑1 (MQ=255)   tCACTTCAGGGAAGAGAGATAATGAGAGCAGCTCTATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGCACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCg                                                                                                                                                                                                                             >  2:385791/1‑250 (MQ=255)    cACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGCGATCCGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGACTAACTTTCCCGGACTGGCCCGAACGCATAGCGTAAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCg                                                                                                                                                                                                                             >  2:374378/1‑249 (MQ=255)                 agagaTAATGAGAGCATCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATTCCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATc                                                                                                                                                                                                              <  2:314761/251‑1 (MQ=255)                          gagaGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCgccgcc                                                                                                                                                                                                     <  2:136722/251‑1 (MQ=255)                               cagcTATATAAGCGATCAACAAAATTACGAACTCTTTATTACCCTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCACAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGCGAACGAGGCCAGACTGAATCAGCGCCGCCATCtt                                                                                                                                                                                                >  2:308876/1‑251 (MQ=255)                                agcTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCACTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTg                                                                                                                                                                                               >  1:248236/1‑251 (MQ=255)                                      ttaAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGCAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTAcc                                                                                                                                                                                           >  1:8244/2‑249 (MQ=255)                                                                            gACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAAt                                                                                                                                                      <  1:397567/248‑1 (MQ=255)                                                                            gACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGATTCAAACATCTCACGACCGTAAATGCCTTTAAt                                                                                                                                                      >  2:397567/1‑248 (MQ=255)                                                                                ggATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGACCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACa                                                                                                                                                 >  1:433852/1‑249 (MQ=255)                                                                                 ggttttACATATTTAACAGAGTAACCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAAGCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCTATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGACTCAGCGCCGCCATCTTGTACCAGGTTGCAAACATCTCACGACCGTAAATTCCTTTAATGAACAAGc                                                                                                                                              <  2:277107/249‑1 (MQ=255)                                                                                             ttAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCAGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGCGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATc                                                                                                                                   >  1:176895/1‑250 (MQ=255)                                                                                                      tATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTcc                                                                                                                         >  2:97667/1‑251 (MQ=255)                                                                                                              gATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTctatc                                                                                                                 >  1:29469/1‑247 (MQ=255)                                                                                                                             aTACCAGCCCTTGTTAGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACAGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGaaggg                                                                                                  >  2:323839/1‑247 (MQ=255)                                                                                                                               aCCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGCCCGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGaaggtgg                                                                                                >  2:23713/1‑245 (MQ=255)                                                                                                                                            cGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGAGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGCATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATc                                                                                   >  1:51285/1‑251 (MQ=255)                                                                                                                                                                        cccGGACTGGCCCGAACGCATAGCTTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACAGCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAGGGTGAAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGgtgt                                                       <  2:289032/251‑1 (MQ=255)                                                                                                                                                                        cccGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGCTCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGGGGTTCATGgtgt                                                       >  2:332170/1‑251 (MQ=255)                                                                                                                                                                            gACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTcg                                                     >  1:181386/1‑248 (MQ=255)                                                                                                                                                                                ggCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTCTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCAAGCATg                                               >  1:46970/1‑251 (MQ=255)                                                                                                                                                                                       aCGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTcc                                                                                                                         <  1:187740/170‑1 (MQ=255)                                                                                                                                                                                       aCGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGCAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTcc                                                                                                                         >  2:187740/1‑170 (MQ=255)                                                                                                                                                                                           aTAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCAc                                                                                                                                 >  1:83038/1‑158 (MQ=255)                                                                                                                                                                                           aTAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCAc                                                                                                                                 <  2:83038/158‑1 (MQ=255)                                                                                                                                                                                              gCGTCAAAGCCCTTCGGGAAAGCATCGATAGAGAACCGATGAGAAATGAGCGGCGAGAGATCGAGGCCAGACTGAATCAGCCCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGACCACTTTTGTCCAGGCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTgg                                 <  2:433852/251‑1 (MQ=255)                                                                                                                                                                                               cGTCAAAGCCCTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATCCCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTggc                                <  1:97667/251‑1 (MQ=255)                                                                                                                                                                                                 tCAAAGCCCTTCTGGACATCATCGATAGAGCACCGATGAGTAATGATCGGCGTGAGATCGAGGCCAGACTGAATCAGCGCCGCCCTCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATAc                                                                                           >  1:376517/1‑190 (MQ=255)                                                                                                                                                                                                 tCAAAGCCCGTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCGTTAATGAACAAGCCCGTAAAGATCACTTTTGTCCAGTCTATCGACATGGCAGAAGGTGGAATAc                                                                                           <  2:376517/190‑1 (MQ=255)                                                                                                                                                                                                       cccTTCTGGAAATCATCGATAGAGAACCGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCAcc                        >  1:187316/1‑251 (MQ=255)                                                                                                                                                                                                                    catcGATAGAGACCCGATGAGTACTGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCAATCTTGTACCCGGTTTCAAACATCTCACGCCCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGTGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGTGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAgc           >  2:37800/1‑250 (MQ=255)                                                                                                                                                                                                                              gaaccGATGAGTAATGATCGGCGAGAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAACCATCTCACGACCGTAAATGCCTTTAATGAACAAGCCCTTAAAGATCACTTTTGTCCAGTCTATCGACATGTCAGAAGGCGGAATACCCAGCATCGCAATACGACCGCCGTGGTTCATGGGGTCGAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACAtac  >  2:240831/5‑248 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                 AGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCGAACGCATAGCGTCAAAGCCCTTCTGGAAATCATCGATAGAGAAACGATGGGTAATGATCGGCGAAAGATCGAGGCCAGACTGAATCAGCGCCGCCATCTTGTACCAGGTTTCAAACATCTCACGACCGTAAATACCTTTAATGAACAAGCCTTTAAAGATCACTTTGGTCCAGTCGATAGACATATCAGACGGCGGAATACCCAGCATCGCAATACGGCCGCCGTGATTCATGGTGTCAAGCATGGTACGAAACGCTGGCGGCGCACCGGACATTTCCAGACCGACATCA  >  NC_000913/3790177‑3790647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑