Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,789,919	A→G	Y55H (TAT→CAT)	waaH ←	UDP‑glucuronate:LPS(HepIII) glycosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,789,919	0	A	G	100.0%	117.7 / NA	35	Y55H (TAT→CAT)	waaH	UDP‑glucuronate:LPS(HepIII) glycosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/24);  total (11/24)

TGCCAGGTTTCTCCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCCATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTGCGGAAATCATCGCCCGCATTATATAACGGAATAATAACACTAAGTTTATTGGTGCTGTTCATCATAAATTACCGACGACCTCTGAAGTTATCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTA  >  NC_000913/3789681‑3790160                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                  tGCCAGGTTTCTCCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGGCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGAGAGTTTTCTGCTTAATGCTTTGCTAttt                                                                                                                                                                                                                                        <  2:143355/250‑1 (MQ=255)    cAGGTTTCTCCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAAAGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAAc                                                                                                                                                                                                                                    >  2:294716/1‑251 (MQ=255)           ctcCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAAtt                                                                                                                                                                                                                              >  1:124371/1‑250 (MQ=255)               cGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTGTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCg                                                                                                                                                                                                                         <  2:100223/251‑1 (MQ=255)                  ttCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGtt                                                                                                                                                                                                                       <  1:330027/250‑1 (MQ=255)                    cACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGaa                                                                                                                                                                                                                    <  1:368637/251‑1 (MQ=255)                       gAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCa                                                                                                                                                                                                                 <  2:373896/251‑1 (MQ=255)                         aaaCACCAGTCAGCGTTGCACTGCGCCACGTCGAGTTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTGCTGCGTAATGCTGTGCTATTTCAACAGAATTATCCGTTGAACCATc                                                                                                                                                                                                               <  1:394528/251‑1 (MQ=255)                             acCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTta                                                                                                                                                                                                           <  1:365559/251‑1 (MQ=255)                               cAGTCAGCGTTGCACTGCGCCCCGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGGTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTtaat                                                                                                                                                                                                         <  1:338971/251‑1 (MQ=255)                                    aGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTtaataata                                                                                                                                                                                                     >  1:78490/1‑250 (MQ=255)                                     gCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTTAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGGTGAACCATCGTTaataataat                                                                                                                                                                                                   <  2:292960/251‑1 (MQ=255)                                       gTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATg                                                                                                                                                                                                  >  1:136722/1‑250 (MQ=255)                                          gCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTTATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTGTGCTATTTCAACAGAAGTATCCGTTGAACCATCGTTAATAATAATGAtt                                                                                                                                                                                               <  2:24115/250‑1 (MQ=255)                                          gCACTGCGCCACGTCGAGGTCGTCCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAAGAATGAttt                                                                                                                                                                                              <  1:114158/251‑1 (MQ=255)                                                           ggTCGTCCTCGAACGCTATGGTCAGCAGCGTTGCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGtt                                                                                                                                                                             <  2:157897/251‑1 (MQ=255)                                                            gtcgtcCTCTAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCTCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTTAACCATCGTGAATAATAATGATTTCCAGAGCAGTCCAGGttt                                                                                                                                                                            <  2:73411/251‑1 (MQ=255)                                                                   tctAACGCTATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAAt                                                                                                                                                                     >  2:280128/1‑251 (MQ=255)                                                                                 catcaGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCTACATATTTGCCCGTTTCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAAGAATGATTACCAGAGCAGTCCAGTTTTTCGTAATTAAATTTTCCATa                                                                                                                                                        <  2:52115/250‑1 (MQ=255)                                                                                  atcaGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATAGTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATGCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTGCATACa                                                                                                                                                      <  2:76236/251‑1 (MQ=255)                                                                                    caGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCTGCTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATGCGCCTGATTCAACAAACGAACGTGCGGATAGGTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCACCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAAGTAAATATTCCATACAAg                                                                                                                                                    <  2:424746/251‑1 (MQ=255)                                                                                      gCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTg                                                                                                                                                  >  2:124175/1‑251 (MQ=255)                                                                                       cGTTTCGTTCATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTGc                                                                                                                                                 >  1:46624/1‑251 (MQ=255)                                                                                       cGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTGc                                                                                                                                                 >  1:28539/1‑251 (MQ=255)                                                                                                                  agcGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTGCAATCCCACGTTTACGCGACACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAAAAGAATTATCCGTTGAACCATCGTTAATAATAATGAGTTCCAGAGCAGTCCAGGTTTGCGTAAGTAAAGATTCCATCCAATTGCGGAAATCATCGCCCGCCTTATATAACg                                                                                                                      <  1:382521/249‑1 (MQ=255)                                                                                                                    cGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTGCGGAAATCATCGCCCGCATTATATAACGGa                                                                                                                    <  1:43393/251‑1 (MQ=255)                                                                                                                                                         cACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTGCGGAAATCATCGCCCGCATTATATAACGGAATAATAACACTAAGTTTATTGGTGCTGTTCATCATaa                                                                               <  1:161990/251‑1 (MQ=255)                                                                                                                                                              cAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTGCGGAAATCATCGCCCGCATTATATAACGGAATAATAACACTAAGTTTATTGGTGCTGTTCATCATAAATTa                                                                           <  2:124371/250‑1 (MQ=255)                                                                                                                                                                aTCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTGCGGAAATCATCGCCCGCATTATATAACGGAATAATAACACTAAGTTTATTGGTGCTGTCCATCATAAATTACcg                                                                        >  1:289032/1‑251 (MQ=255)                                                                                                                                                                   ccACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGACCGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAACGATTCCATACACGTGCGGAAATCATCGCCCGCATTATATAACGGAATAATAACACTAAGTTTATTGGTGCTGTTCATCATAAATTACcgacg                                                                     >  1:277107/1‑251 (MQ=255)                                                                                                                                                                                    cGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTGCGGAAATCATCGCCCGCATTATATAACGGAATAATAACACTAAGTTTATTGGTGCTGTTCATCATAAATTACCGACGACCTCTGAAGTTATCCt                                                    <  1:321699/251‑1 (MQ=255)                                                                                                                                                                                            cAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTGCGGAAATCATCGCCCGCATTATATAACGGAATAATAACACTAAGTTTATTGGTGCTGTTCATCATAAATTACCGACGACCTCTGAAGTGATCCTGACGtttt                                            <  2:78490/251‑1 (MQ=255)                                                                                                                                                                                                aTTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTGCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAAGGATTTCCAGAGCAGTCCGTGTTTGCGTAATTAAAGATTCCAGACAAGTGCGGAAATCATTGCCCGCAGTATATAACGGAATAATAACACGAAGTGTATTGGTGCTTTTCATCATGAATTACCGACGTCCATGGAAGTTAGCCTGACTTTTTACAg                                        <  2:214358/251‑1 (MQ=255)                                                                                                                                                                                                                 aacgaacgTGCGGATAGTTTTCTGCGTAATGCTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTGCGGAAATCATCGCCCGCATTATATAACGGAATAATAACACTAAGTTTATTGGTGCTGTTCATCATAAATTACCGACGACCTCTGAAGTTATCCTGACGTTTTACAGGCTTTGTATTAAGAAAc                       >  2:66505/1‑251 (MQ=255)                                                                                                                                                                                                                                        gCGTAATGCGTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTGCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTGCGGAAATCATCGCCCGCATTATATAACGGAATAATAACACTAAGTTTATTGGTGCTGTTCATCATAAATTACCGACGACCTCTGAAGTTATCCTGACGTTTTACAGGCTTTGTATTAAGAAACGATTAAGCCTGTGAGAtatta  <  2:28539/249‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                  TGCCAGGTTTCTCCCGTTTCACGAAAACACCAGTCAGCGTTGCACTGCGCCACGTCGAGGTCGTCCTCTAACGCCATGGTCATCAGCGTTTCGTACATGGTGGGATAGACTTCATCGTCAGCATCGACAAAAGCGACATATTTGCCCGTTGCCACTTCAATCCCACGATTACGCGCCACCGATGCGCCAGCATTCGCCTGATGCAACAAACGAACGTGCGGATAGTTTTCTGCGTAATACTTTGCTATTTCAACAGAATTATCCGTTGAACCATCGTTAATAATAATGATTTCCAGAGCAGTCCAGGTTTGCGTAATTAAAGATTCCATACAAGTGCGGAAATCATCGCCCGCATTATATAACGGAATAATAACACTAAGTTTATTGGTGCTGTTCATCATAAATTACCGACGACCTCTGAAGTTATCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTA  >  NC_000913/3789681‑3790160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑