Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,789,479	G→A	N201N (AAC→AAT)	waaH ←	UDP‑glucuronate:LPS(HepIII) glycosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,789,479	0	G	A	96.0%	70.1 / ‑5.7	25	N201N (AAC→AAT)	waaH	UDP‑glucuronate:LPS(HepIII) glycosyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (17/7);  minor base C (1/0);  total (18/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

CTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAA‑TTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCGTTAAACATGAATTCTGTTGTCCAGACAATATCCTGATGATGTAATCCGGCAATAAATTTAATGTTATTTTTAACAATAACATCACGGCGATAAACCCCCATCCAGACAACGTGAGTCCAACGGCGCGAAGAAAGCCCCATCCGCAGCCAGTCCGGGCCGGTTAATACGCCGGTTGAGCGAAGGCGATCGGTGGGGATGGATTGCCAGGTTTCTCCCG  >  NC_000913/3789241‑3789696                                                                                                                                                                                                                                                |                                                                                                                                                                                                                          ctTTGCGCACCGCATGGCAAAGACGCAAGGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAACTCGTAA‑TTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATTCAACCTACTCACTGTCGTATTATGCATATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCTGCGCATTAAGCATGa                                                                                                                                                                                                                 <  2:250811/249‑1 (MQ=255)     gcgcACCGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAA‑TTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCATTAAACATGAATTCtg                                                                                                                                                                                                           <  2:123377/251‑1 (MQ=255)           cGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAA‑TTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCATTAAACATGAAATCTGTTGTcc                                                                                                                                                                                                     >  1:100223/1‑251 (MQ=255)                ggCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAA‑TTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCATTAAACATGAATTCTGTTGTCCAGaa                                                                                                                                                                                                 >  1:380229/1‑249 (MQ=255)                   aaaCACGCAATGCTTCGTAAGTTATTTGCTGCTGAAATTCCGGATAAATCGTAA‑TTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCATTAAACATGAATTCTGTTGTCCAGACAat                                                                                                                                                                                              >  1:24115/1‑250 (MQ=255)                            aTGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAA‑TTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCATTAAACATGAATTCTGTTGTCCAGACAATATCCtgatg                                                                                                                                                                                     >  2:28225/1‑250 (MQ=255)                                  cGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAA‑TTTTGTCGGCATCATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCATTAAACATGAATTCTGTTGTCCAGACAATATCCTGATGATGTAAt                                                                                                                                                                              >  1:424746/1‑251 (MQ=255)                                          aTTTGCTGATGAAATTCCGGATAAATCGTAA‑TTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCATTAAACATGAATTCTGTTGGCCAGACAATATCCTGATGATGTAATCCGGCaa                                                                                                                                                                       <  2:202015/250‑1 (MQ=255)                                                    gaAATTCCGGATAAATCGTAA‑TTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCATTAAACATGAATTCTGTTGTCCAGACAATATCCTGATGATGTAATCCGGCAATAAATTTAAt                                                                                                                                                             <  1:217567/250‑1 (MQ=255)                                                       aTTCCGGATAAATCGTAAGTTTTG‑CGGCATAATGTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCACTTGGCTATGCAACCGACTCACTGACGTAGTATGCAGATAATATTTATATAATTATTGCTCGTTATATCGCGCACGCAGCGTATTAAACATGAATTCTGTTGGCCAGACAATATCCTGATGATGTAATCCGGCAATAAATTTAATGTTa                                                                                                                                                         <  1:71717/251‑1 (MQ=255)                                                              aTAAATCGTAA‑TTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTGGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCATTAAACATGAATTCTGTTGTCCAGTCAATATCCTGATGATGTAATCCGGCAATAAATTTAATGTTATTGTTaa                                                                                                                                                  <  2:354746/251‑1 (MQ=255)                                                                      tAA‑TTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCATTAAACATGAATTCTGTTGTCCAGACAATATCCTGATGATGTAATCCGGCAATAAATTTAATGTTATTTTTAACAATAAc                                                                                                                                           >  1:312172/1‑250 (MQ=255)                                                                                                            cagGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCATTAAACATGAATTCTGTTGTCCAGACAATATCCTGATGATGTAATCCGGCAATAAATTTAATGTTATTTTTAACAATAACATCGCGGCGATACACCCCCATCCAGACAACGTGAGTcc                                                                                                     >  1:76236/1‑251 (MQ=255)                                                                                                                                  tGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCATTAAACATGAATTCTGTTGTCCAGACAATATCCTGATGATGTAATCCGGCAATAAATTTAATGTTATTTTTAACAATAACATCGCGGCGATAAACCCCCATCCAGACAACGTGAGTCCAACGGCGCGAAGAACGCCCCAt                                                                               >  1:157897/1‑251 (MQ=255)                                                                                                                                             aTTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCATTAAACATGAATTCTGTTGTCCAGACAATATCCTGATGATGTAATc                                                                                                                                                                             >  2:318505/1‑146 (MQ=255)                                                                                                                                             aTTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCATTAAACATGAATTCTGTTGTCCAGACAATATCCTGATGATGTAATc                                                                                                                                                                             <  1:318505/146‑1 (MQ=255)                                                                                                                                                           tCCCTTGTCTATGCATCCGACTCACTGACGTATTATGCAGATAATCTTTATATCATGATTGCTCGGTGTATCGCGCACGCAGCGCATTAAACATGCATTCTGTTGTCCAGACAATATCCTGATGATGAAATCCGGCAATAACTTTAATGTTATTTTTAACAATAACATCGCGGCGATAAACCCCCATCCAGACAACGTGAGTCCAACGGCGCGCAGAAAGCCCCATCCGCCGCCAGTCCGGGCCGGATAAt                                                      >  2:394528/1‑251 (MQ=255)                                                                                                                                                             ccTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCATTAAACATGAATTCTGTTGTCCAGACAATATCCTGATGATGTAATCCGGCAATAAATTTAATGTTATTTTTAACAATAACATCGCGGCGATAAACCCCCATCCAGACAACGTGAGTCCAACGGCGCGAAGAAAGCCCCATCCGCAGCCAGTCCGGGCCGGTTAATAc                                                    >  1:292960/1‑251 (MQ=255)                                                                                                                                                                                tCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCATTAAACATGAATTCTGTTGTCCAGACAATATCCTGATGATGTAATCCGGCAATAAATTTAATGTTATTTTTAACAATAACATCGCGGCGATAAACACCCATCCAGACAACGTGAGTCCAACGGCGCGAAGAAAGCCCCATCCGCAGCCAGTCCGGGCCGGTTAATACACCGGTTGAGCGAAGGCg                                  >  2:353798/1‑250 (MQ=255)                                                                                                                                                                                    tGACGTATTATGCCGATAATATTTATATAATGATTGCTCGGTATATCGCGCCCGCAGCGCATTAAACATGAATTCTGTTGTCCAGACAATATCCTGCTGATGTAATCCGGCAATAAATTTAATGTTATTTTTAACCATACCATCGCGGCGATAAACCCCCATCCAGACAACGTGAGTCCAACGGCGCGAAGACAGCCCCATCCGCAGCCAGTCCGGGCCGGTTACTACACCGGTTGAGCGAAGGCGATCgc                             >  1:214358/1‑250 (MQ=255)                                                                                                                                                                                            tatGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCATTAAACATGAATTCTGTTGTCCAGACAATATCCTGATGATGTAATCCGGCAATAAATTTAATGTTATTTTTAACAATAACATCGCGGCGATAAACCCCCATCCAGACAACGTGAGTCCAACGGCGCGAAGAAAGCCCCATCCGCAGCCAGTCCGGGCCGGTTAATACACCGGTTGAGCGAAGGCGATCGCTGGggatg                     >  1:129354/1‑251 (MQ=255)                                                                                                                                                                                               gCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCATTAAACATGAATTCTGTTGTCCAGACAATATCCTGATGATGTAATCCGGCAATAAATTTAATGTTATTTTTAACAATAACATCGCGGCGATAAACCCCCATCCAGACAACGTGAGTCCAACGGCGCGAAGAAAGCCCCATCCGCAGCCAGTCCGGGCCGGTTAATACACCGGTTGAGCGAAGGCGATCGCTGGggatggat                  >  2:368637/1‑251 (MQ=255)                                                                                                                                                                                                    taataTTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCATTAAACATGAATTCTGTTGTCCAGACAATATCCTGATGATGTAATCCGGCAATAAATTTAATGTTATTTTTAACAATAACATCGCGGCGATAAACCCCCATCCAGACAACGTGAGTCCAACGGCGCGAAGAAAGCCCCATCCGCAGCCAGTCCGGGCCGGTTAATACACAGGTTGAGCGAAGGCGATCGCTGGGGATGGATTGcc              >  1:314761/1‑250 (MQ=255)                                                                                                                                                                                                             tataATGATTGCTCGGTATATCGAGCAAGCAGCGCCTTAAACCTGAATTCTGTTCTCCAGACAATATCCTGCTGATGTAATACGGCAATAAATTTAATGTTATTTTTAACAATAACATCGCGGCGATAAACCCCCATCCAGACAACGTGAGTCAAACGGCGCGAAGAAAGCCCCCACCACAGCCAGTCCGGGCAGGTTAATACACCGGATGAGCGCAACCGCTCGCTGTGGATGGCTTGCCAGGTTtcccc    >  2:382521/1‑248 (MQ=255)                                                                                                                                                                                                               taATGATTGCTCGGTATATCGCGCACGCAGCGCATTAAACATGAATTCTGTTGTCCAGACAATATCCTGATGATGTAATCCGGCAATAAATTTAATGTTATTTTTAACAATAACATCGCGGCGATAAACCCCCATCCAGACAACGTGAGTCCAACGGCGCGAAGAAAGCCCCATCCGCAGCCAGTCCGGGCCGGTTAATACACCGGTTGAGCGAAGGCGATCGCTGGGGATGGATTGCCAGGTTTCTCCCg  >  1:270491/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                          CTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAA‑TTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCGTTAAACATGAATTCTGTTGTCCAGACAATATCCTGATGATGTAATCCGGCAATAAATTTAATGTTATTTTTAACAATAACATCACGGCGATAAACCCCCATCCAGACAACGTGAGTCCAACGGCGCGAAGAAAGCCCCATCCGCAGCCAGTCCGGGCCGGTTAATACGCCGGTTGAGCGAAGGCGATCGGTGGGGATGGATTGCCAGGTTTCTCCCG  >  NC_000913/3789241‑3789696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑