Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,789,309	A→G	M258T (ATG→ACG)	waaH ←	UDP‑glucuronate:LPS(HepIII) glycosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,789,309	0	A	G	100.0%	109.7 / NA	32	M258T (ATG→ACG)	waaH	UDP‑glucuronate:LPS(HepIII) glycosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/19);  total (13/19)

GCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAA‑TTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCGTTAAACATGAATTCTGTTGTCCAGACAATATCCTGATGATGTAATCCGGCAATAAATTTAATGTTATTTTTAA  >  NC_000913/3789065‑3789552                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     gcTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCAACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGATCTTTGCGCAACGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAA‑ttt                                                                                                                                                                                                                                               >  1:287523/1‑251 (MQ=255)    aCGCGTAATGCGATGGTGCGACAGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCCACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCTCCGCATGGCAAACACGCAATGCTTCGTAAGTCATTTGCTTATTAAATTCCGGATAATTCGTAA‑TTTTg                                                                                                                                                                                                                                             <  2:157827/250‑1 (MQ=255)     cgcgTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCCACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAA‑TTTTGTc                                                                                                                                                                                                                                           <  1:252910/251‑1 (MQ=255)                aTGGGGCGACCGCGTTTTGTCGCGCCTTTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCCACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCAACGCATGGCAAACACGCAATGATTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAA‑TTTTGTCGGCATAATTTc                                                                                                                                                                                                                                >  2:114158/1‑251 (MQ=255)                  ggTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCCACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAA‑TATTGTCGGCATAATTTCg                                                                                                                                                                                                                               <  2:255399/250‑1 (MQ=255)                      cGACCGCGTTTTGTCGCGCCATTGACATAAGCGGAAAGACCACAGTAACGCCTGGTAACCCACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAA‑TGTTGTCGGCATAATTTCGATTTa                                                                                                                                                                                                                          <  1:129395/251‑1 (MQ=255)                                                     cGGAAAGACCACAGTAACGCCTGGTAACCCACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAA‑TTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAatat                                                                                                                                                                                            >  1:52115/1‑250 (MQ=255)                                                        aaaGACCACAGTAACGCCTGGTAACCCACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAA‑TTTTGTCGGCATAATTTCGATTTAAGTTCTCCAGCAGGCGGGTAATCTTAATATAGTg                                                                                                                                                                                        <  1:311199/251‑1 (MQ=255)                                                           gACCACAGTAACGCCTGGTAACCCACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAA‑TTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACg                                                                                                                                                                                     <  1:136369/251‑1 (MQ=255)                                                                 aGTAACGCCTGGTAACCCACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAA‑TTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTGATATAGTGACGTTGATa                                                                                                                                                                               <  2:85418/251‑1 (MQ=255)                                                                        ccTGGTAACCCACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAA‑TTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAgg                                                                                                                                                                        <  2:120997/251‑1 (MQ=255)                                                                            gTAACCCACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAA‑TTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGtttt                                                                                                                                                                    <  1:312198/251‑1 (MQ=255)                                                                                         aCGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAA‑TTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCt                                                                                                                                                       <  1:116614/251‑1 (MQ=255)                                                                                                               ggCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGCGCTTTGCGCACCGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAA‑TTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTAGGCATCCGACTCACTGACGta                                                                                                                                 <  2:30684/251‑1 (MQ=255)                                                                                                                        acatacCGGAAGGAAATATCTCGGCAATCATCCGTTGTCGGGTAAGAATATCCGTCTCTTTTCGCACCGCAGGGCAAACACGCGATGCTTCGAAAAATATTTGCTGATGAAATTCCGGATAAATCGTAA‑TTTTGTCGGCATAATTTCGATTTAATTTCTCGAGCGGGCGGGGCATCTTAATCTAATTCCTTTGATAATTTAGGATTTTGTTCCCTTTTCTATGCAACCAACTCACTGACGtattgttcaa                                                                                                                         >  2:338971/1‑244 (MQ=255)                                                                                                                           tacCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAA‑TTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTTAGGTTTTTATTCCCTTTTCTATTCAACCGACTCACTGACGTATTATGCAGata                                                                                                                      >  2:319465/1‑250 (MQ=255)                                                                                                                                gAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAA‑TTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTTCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATAttt                                                                                                                >  2:430624/1‑251 (MQ=255)                                                                                                                                         atCTCTGCAATCATCCGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATAGTAA‑TTTGGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGGGACGTTGATAATTAAGTTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAAtt                                                                                                        <  2:12818/250‑2 (MQ=255)                                                                                                                                                            ggCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAA‑TTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCgcgcc                                                                                    >  1:314166/1‑250 (MQ=255)                                                                                                                                                                                 ctTTGCGCACCGCATGGCAAAGACGCAAGGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAACTCGTAA‑TTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATTCAACCTACTCACTGTCGTATTATGCATATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCTGCGCATTAAGCATGa                                                                 <  2:250811/249‑1 (MQ=255)                                                                                                                                                                                     gcgcACCGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAA‑TTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCATTAAACATGAATTCtg                                                           <  2:123377/251‑1 (MQ=255)                                                                                                                                                                                           cGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAA‑TTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCATTAAACATGAAATCTGTTGTcc                                                     >  1:100223/1‑251 (MQ=255)                                                                                                                                                                                                ggCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAA‑TTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCATTAAACATGAATTCTGTTGTCCAGaa                                                 >  1:380229/1‑249 (MQ=255)                                                                                                                                                                                                   aaaCACGCAATGCTTCGTAAGTTATTTGCTGCTGAAATTCCGGATAAATCGTAA‑TTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCATTAAACATGAATTCTGTTGTCCAGACAat                                              >  1:24115/1‑250 (MQ=255)                                                                                                                                                                                                            aTGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAA‑TTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCATTAAACATGAATTCTGTTGTCCAGACAATATCCtgatg                                     >  2:28225/1‑250 (MQ=255)                                                                                                                                                                                                                 tCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAA‑TTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTcc                                                                                                                                                              <  2:305414/124‑1 (MQ=255)                                                                                                                                                                                                                 tCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAA‑TTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTcc                                                                                                                                                              >  1:305414/1‑124 (MQ=255)                                                                                                                                                                                                                  cGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAA‑TTTTGTCGGCATCATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCATTAAACATGAATTCTGTTGTCCAGACAATATCCTGATGATGTAAt                              >  1:424746/1‑251 (MQ=255)                                                                                                                                                                                                                          aTTTGCTGATGAAATTCCGGATAAATCGTAA‑TTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCATTAAACATGAATTCTGTTGGCCAGACAATATCCTGATGATGTAATCCGGCaa                       <  2:202015/250‑1 (MQ=255)                                                                                                                                                                                                                                    gaAATTCCGGATAAATCGTAA‑TTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCATTAAACATGAATTCTGTTGTCCAGACAATATCCTGATGATGTAATCCGGCAATAAATTTAAt             <  1:217567/250‑1 (MQ=255)                                                                                                                                                                                                                                       aTTCCGGATAAATCGTAAGTTTTG‑CGGCATAATGTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCACTTGGCTATGCAACCGACTCACTGACGTAGTATGCAGATAATATTTATATAATTATTGCTCGTTATATCGCGCACGCAGCGTATTAAACATGAATTCTGTTGGCCAGACAATATCCTGATGATGTAATCCGGCAATAAATTTAATGTTa         <  1:71717/251‑1 (MQ=255)                                                                                                                                                                                                                                              aTAAATCGTAA‑TTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTGGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCATTAAACATGAATTCTGTTGTCCAGTCAATATCCTGATGATGTAATCCGGCAATAAATTTAATGTTATTGTTaa  <  2:354746/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                     GCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAA‑TTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCGTTAAACATGAATTCTGTTGTCCAGACAATATCCTGATGATGTAATCCGGCAATAAATTTAATGTTATTTTTAA  >  NC_000913/3789065‑3789552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑