Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,789,272	C→T	E270E (GAG→GAA)	waaH ←	UDP‑glucuronate:LPS(HepIII) glycosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,789,272	0	C	T	100.0%	90.2 / NA	28	E270E (GAG→GAA)	waaH	UDP‑glucuronate:LPS(HepIII) glycosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/17);  total (11/17)

AGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCGTTAAACATGAATTCTGTTGTCCAGACAATATCCTGATG  >  NC_000913/3789038‑3789517                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                      aacttAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCCACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCt                                                                                                                                                                                                                                       <  1:16008/248‑1 (MQ=255)                      aaaGGCGCTACGCGTAAAGCGATGGTGCGACCGCGTGTTGTCGCGCCATTGCCAGAAGCGGAAAGACCCCAGTAACGCCTGGTAACCCCCCGTCACGCCGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGCTTTTTGCGCACCGCATGGCAAACACGCAAGGCTTAGTAAGTGATTTGCTGATGAAATTCCGGATAAATc                                                                                                                                                                                                                   <  2:240472/250‑1 (MQ=255)                       aaGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCCACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGTATAAATcgt                                                                                                                                                                                                                 <  1:11314/251‑3 (MQ=255)                            gcTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCAACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGATCTTTGCGCAACGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAAttt                                                                                                                                                                                                            >  1:287523/1‑251 (MQ=255)                               aCGCGTAATGCGATGGTGCGACAGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCCACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCTCCGCATGGCAAACACGCAATGCTTCGTAAGTCATTTGCTTATTAAATTCCGGATAATTCGTAATTTTg                                                                                                                                                                                                          <  2:157827/250‑1 (MQ=255)                                cgcgTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCCACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAATTTTGTc                                                                                                                                                                                                        <  1:252910/251‑1 (MQ=255)                                           aTGGGGCGACCGCGTTTTGTCGCGCCTTTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCCACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCAACGCATGGCAAACACGCAATGATTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAATTTTGTCGGCATAATTTc                                                                                                                                                                                             >  2:114158/1‑251 (MQ=255)                                             ggTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCCACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAATATTGTCGGCATAATTTCg                                                                                                                                                                                            <  2:255399/250‑1 (MQ=255)                                                 cGACCGCGTTTTGTCGCGCCATTGACATAAGCGGAAAGACCACAGTAACGCCTGGTAACCCACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAATGTTGTCGGCATAATTTCGATTTa                                                                                                                                                                                       <  1:129395/251‑1 (MQ=255)                                                                                cGGAAAGACCACAGTAACGCCTGGTAACCCACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAatat                                                                                                                                                         >  1:52115/1‑250 (MQ=255)                                                                                   aaaGACCACAGTAACGCCTGGTAACCCACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAATTTTGTCGGCATAATTTCGATTTAAGTTCTCCAGCAGGCGGGTAATCTTAATATAGTg                                                                                                                                                     <  1:311199/251‑1 (MQ=255)                                                                                      gACCACAGTAACGCCTGGTAACCCACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACg                                                                                                                                                  <  1:136369/251‑1 (MQ=255)                                                                                            aGTAACGCCTGGTAACCCACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTGATATAGTGACGTTGATa                                                                                                                                            <  2:85418/251‑1 (MQ=255)                                                                                                   ccTGGTAACCCACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAgg                                                                                                                                     <  2:120997/251‑1 (MQ=255)                                                                                                       gTAACCCACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGtttt                                                                                                                                 <  1:312198/251‑1 (MQ=255)                                                                                                                    aCGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCt                                                                                                                    <  1:116614/251‑1 (MQ=255)                                                                                                                                          ggCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGCGCTTTGCGCACCGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTAGGCATCCGACTCACTGACGta                                                                                              <  2:30684/251‑1 (MQ=255)                                                                                                                                                   acatacCGGAAGGAAATATCTCGGCAATCATCCGTTGTCGGGTAAGAATATCCGTCTCTTTTCGCACCGCAGGGCAAACACGCGATGCTTCGAAAAATATTTGCTGATGAAATTCCGGATAAATCGTAATTTTGTCGGCATAATTTCGATTTAATTTCTCGAGCGGGCGGGGCATCTTAATCTAATTCCTTTGATAATTTAGGATTTTGTTCCCTTTTCTATGCAACCAACTCACTGACGtattgttcaa                                                                                      >  2:338971/1‑244 (MQ=255)                                                                                                                                                      tacCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTTAGGTTTTTATTCCCTTTTCTATTCAACCGACTCACTGACGTATTATGCAGata                                                                                   >  2:319465/1‑250 (MQ=255)                                                                                                                                                           gAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTTCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATAttt                                                                             >  2:430624/1‑251 (MQ=255)                                                                                                                                                                    atCTCTGCAATCATCCGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATAGTAATTTGGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGGGACGTTGATAATTAAGTTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAAtt                                                                     <  2:12818/250‑2 (MQ=255)                                                                                                                                                                                       ggCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCgcgcc                                                 >  1:314166/1‑250 (MQ=255)                                                                                                                                                                                                            ctTTGCGCACCGCATGGCAAAGACGCAAGGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAACTCGTAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATTCAACCTACTCACTGTCGTATTATGCATATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCTGCGCATTAAGCATGa                              <  2:250811/249‑1 (MQ=255)                                                                                                                                                                                                                gcgcACCGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCATTAAACATGAATTCtg                        <  2:123377/251‑1 (MQ=255)                                                                                                                                                                                                                      cGCATGGCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCATTAAACATGAAATCTGTTGTcc                  >  1:100223/1‑251 (MQ=255)                                                                                                                                                                                                                           ggCAAACACGCAATGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCATTAAACATGAATTCTGTTGTCCAGaa              >  1:380229/1‑249 (MQ=255)                                                                                                                                                                                                                              aaaCACGCAATGCTTCGTAAGTTATTTGCTGCTGAAATTCCGGATAAATCGTAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCATTAAACATGAATTCTGTTGTCCAGACAat           >  1:24115/1‑250 (MQ=255)                                                                                                                                                                                                                                       aTGCTTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCGTAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCATTAAACATGAATTCTGTTGTCCAGACAATATCCtgatg  >  2:28225/1‑250 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                      AGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCGCTTATACATACCGGAAGTAAATATCTCGGCAATCATCCGTTGGCGGGTAAGAATATCCGGCTCTTTGCGCACCGCATGGCAAACACGCAATGCCTCGTAAGTTATTTGCTGATGAAATTCCGGATAAATCATAATTTTGTCGGCATAATTTCGATTTAATTTCTCCAGCAGGCGGGTAATCTTAATATAGTGACGTTGATAATTAAGGTTTTTATTCCCTTGTCTATGCAACCGACTCACTGACGTATTATGCAGATAATATTTATATAATGATTGCTCGGTATATCGCGCACGCAGCGCGTTAAACATGAATTCTGTTGTCCAGACAATATCCTGATG  >  NC_000913/3789038‑3789517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑