Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,788,523	G→A	A141A (GCG→GCA)	yibQ →	divergent polysaccharide deacetylase domain‑containing protein YibQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,788,523	0	G	A	100.0%	202.6 / NA	60	A141A (GCG→GCA)	yibQ	divergent polysaccharide deacetylase domain‑containing protein YibQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/36);  total (24/36)

CAGAGAGATGGCGACCAAAGCGCATAACAGCGGGCACGAAGTGTTGATTCATCTCCCGATGGCACCGTTGAGTAAACAGCCGCTGGAGAAAAATACGCTACGCCCGGAGATGAGCAGCGACGAAATTGAGCGCATTATTCGTAGTGCGGTCAATAACGTGCCCTATGCCGTGGGGATCAACAACCACATGGGCAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCGCTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGCGCGCCGCGCAAGGCACTGGCGTGAAGGTGATCAAACGGAAGGTATTCCTCGACGATTCGCAAAATGAAGCGGACATCCGTGTGCAATTTAATCGCGCAATTGACCTGGCGCGTCGCAACGGTTCGACCATTGCCATTGGGCATCCTCACCCTGCAACGGTACGCGTGTTGCAACAGATGGTTTAT  >  NC_000913/3788283‑3788769                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                       cAGAGAGATGGCGACCAAAGCGCATAACAGCGGGCACGAAGTGTTGATTCATCTCCCGATGGCACCACTGAGTAAACAGCCGCTGGAGAAAAATACGCTGCGCCCGGAGATGAGCAGCGACGAAATTGAGCGCATTATTCGTAGTGCGGTCAATAACGTGCCCTATGCCGTGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGtt                                                                                                                                                                                                                                              <  2:240877/251‑1 (MQ=255) cAGAGAGATGGCGACCAAAGCGCATAACAGCGGGCACGAAGTGTTGAGTCATCTCCCGAGGGCACCACTGAGTAAACAGCCGCTGGAGAAAAATACGCTGCGCCCGGAGATGAGCAGCGACGAAATTGAGCGCATTATTCGTAGTGCGGTCAATAACGTGCCCTATGCCGTGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGtt                                                                                                                                                                                                                                              <  2:375220/251‑1 (MQ=255) cAGAGAGATGGCGACCAAAGCGCATAACAGCGGGCACGAAGTGGTGATTCATCTCCCGATGGCACCACTGAGTAAACAGCCGCTGGAGAAAAATACGCTGCGCCCGGAGATGAGCAGCGACGAAATTGAGCGCATTATTCGTAGTGCGGTCAATAACGTGCCCTATGCCGTGGGGAGCAACAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGtt                                                                                                                                                                                                                                              <  2:58687/251‑1 (MQ=255)  agagagATGGCGACCAAAGCGCATAACAGCGGGCACGAAGTGTTGATTCATCTCCCGATGGCACCACTGAGTAAACAGCCGCTGGAGAAAAATACGCTGCGCCCGGAGATGAGCAGCGACGAAATTGAGCGCATTATTCGTAGTGCGGTCAATAACGTGCCCTATGCCGTGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTa                                                                                                                                                                                                                                             <  1:32234/251‑1 (MQ=255)  agagagATGGCGACCAAAGCGCATAACAGCGGGCACGAAGTGTTGATTCATCTCCCGATGGCACCACTGAGTAAACAGCCGCTGGAGAAAAATACGCTGCGCCCGGAGATGAGCAGCGACGAAATTGAGCGCATTATTCGTAGTGCGGTCAATAACGTGCCCTATGCCGTGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTa                                                                                                                                                                                                                                             <  2:400136/251‑1 (MQ=255)   gagagaTGGCGACCAAAGCGCATAACAGCGGGCACGAAGTGTTGATTCATCTCCCGATGGCACCACTGAGTAAACAGCCGCTGGAGAAAAATACGCTGCGCCCGGAGATGAGCAGCGACGAAATTGAGCGCATTATTCGTAGTGCGGTCAATAACGTGCCCTATGCCGTGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGTGTTAc                                                                                                                                                                                                                                            <  2:284931/251‑1 (MQ=255)          ggCGACCAAAGCGCATAACAGCGGGCACGAAGTGTTGATTCATCTCCCGATGGCACCACTGAGTAAACAGCCGCTGGAGAAAAATACGCTGCGCCCGGAGATGAGCAGCGACGAAATTGAGCGCATTATTCGTAGTGCGGTCAATAACGTGCCCTATGCCGTGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCttt                                                                                                                                                                                                                                     <  1:14222/251‑1 (MQ=255)            cGACCAAAGCGCATAACAGCGGGCACGAAGTGTTGATTCATCTCCCGATGGCACCACTGAGTAAACAGCAGCTGGAGAAAAATACGCTGCGCCCGGAGATGAGCAGCGACGAAATTGAGCGCATTATTCGTAGTGCGGTCAATAACGTGCCCTATGCCGTGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTAc                                                                                                                                                                                                                                   >  1:101628/1‑251 (MQ=255)               ccAAAGCGCATAACAGCGGGCACGAAGTGTTGATTCATCTCCCGATGGCACCACTGAGTAAACAGCCGCTGGAGAAAAATACGCTGCGCCCGGAGATGAGCAGCGACGAAATTGAGCGCATTATTCGTAGTGCGGTCAATAACGTGCCCTATGCCGTGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTc                                                                                                                                                                                                                                >  2:16452/1‑251 (MQ=255)                 aaaGCTCATAACAGCGGGCACGAAGTGTTGATTCATCTCCCGATGGCACCACTGAGTAAACAGCCGCTGGAGAAAAATACGCTGCGCCCGGAGATGAGCAGCGACGAAATTGAGCGCATTATTCGTAGTGCGGTCAATAACGTGCCCTATGCCGTGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCt                                                                                                                                                                                                                              <  2:25908/251‑1 (MQ=255)                 aaaGCGCATAACAGCGGGCACGAAGTGTTGATTCATCTCCCGATGGCACCACTGAGTAAACAGCCGCTGGAGAAAAATGCGCTGCGCCCGGAGATGAGCAGCGACGAAATTGAGCGCATTATTCGTAGTGCGGTCAATAACGTGCCCTATGCCGTGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTGTACTTCCt                                                                                                                                                                                                                              <  2:144163/251‑1 (MQ=255)                  aaGCGCATAACAGCGGGCACGAAGTGTTGATTCATCTCCCGATGGCACCACTGAGTAAACAGCCGCTGGAGAAAAATACGCTGCGCCCGGAGATGAGCAGCGACGAAATTGAGCGCATTATTCGTAGTGCGGTCAATAACGTGCCCTATGCCGTGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTc                                                                                                                                                                                                                             <  1:409862/251‑1 (MQ=255)                      gcATAACCGCGGGCACGAAGTGTTGATTCATCTCCCGATGGCACCACTGAGTAAACAGCCGCTGGAGAAAAATACGCTGCGCACGGAGATGAGCAGCGACGAAATTGAGCGCATTATTCGTAGTGCGGTCAATAACGTGCCCTATGCCGTGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAACGTGATGCCGGCACTGGAGCGTTACAATCTTTACTTCCTCGAc                                                                                                                                                                                                                          >  2:266222/1‑250 (MQ=255)                            cAGCGGGCACGAAGTGTTGATTCATCTCCCGATGGCACCACTGAGTAAACAGCCGCTGGAGAAAAATACGCTGCGCCCGGAGATGAGCAGCGACGAAATTGAGCGCATTATTCGTAGTGCGGTCAATAACGTGCCCTATGCCGTGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTaa                                                                                                                                                                                                                   <  1:289611/251‑1 (MQ=255)                                       aaGTGTTGCTTCATCTCCAGATGGCACCACTGAGTAAACAGCCGCTGGAGAAAAATACGCTGCGCCCGGAGATGAGCAGCGACGAAATTGAGCGCATTATACGTAGTGCGGTCAATAACGTGCCCTATGCCGTGGGGATCAACAACCACATGGGTAGCAAGATGCCCTCTACCCTGTTTTGTCTGCAGAAAGTGATGCAGGCACTGTAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAAt                                                                                                                                                                                                        >  1:271234/1‑251 (MQ=255)                                           gtTGATTCCTCTCCCGCCGGCAACACTGAGTAAAAAGCCGCTGGAGAAAAATACGCTGCGCCCGGCGATGAGCAGCGACGAAATTGAGCGCATTATTCGTAGTGCGGTCAATAACGTGCCCTATGCCGTGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGCAAGTGATGCAGGCACTGGAGCGTTACAATCTTTCCTTCCTCGACAGCGTAACCATCGGTAATAccc                                                                                                                                                                                                    >  1:299311/1‑251 (MQ=255)                                                          gATGGCACCACTGAGTAAACAGCCGCTGGAGAAAACTACGCTGCGCCCGGAGATGAGCAGCGACGCAATTGAGCGCATTATTCGTAGCGCGGTCAATAACGTGCCCTATGCCGTGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGcgcgccg                                                                                                                                                                                     >  1:20887/1‑251 (MQ=255)                                                                accactGAGTAAACAGCCGCTGGAGAAAAATACGCTGCGCCCGGAGATGAGCAGCGACGAAATTGAGCGCATTATTCGTAGTGCGGTCAATAACGTGCCCTATGCCGTGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTc                                                                                                                                                                                                                                >  2:152494/6‑202 (MQ=255)                                                                accactGAGTAAACAGCCGCTGGAGAAAAATACGCTGCGCCCGGAGATGAGCAGCGACGAAATTGAGCGCATTATTCGTAGTGCGGTCAATAACGTGCCCTATGCCGTGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTc                                                                                                                                                                                                                                <  1:152494/197‑1 (MQ=255)                                                                             cAGCCGCTGGAGAAAAATACGCTGCGCCCGGAGATGAGCAGCGACGAAATTGAGCGCATTATTCGTAGTGCGGTCAATAACGTGCCCTATGCCGTGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGCGCGCCGCGCAAGGTACTGGCGTGa                                                                                                                                                                   >  1:397569/1‑250 (MQ=255)                                                                              aGCCGCTGGAGAAAAATACGCTGCGCCCGGAGATGAGCAGCGACGAAATTGAGCGCATTATTCGTAGTGCGGTCAATAACGTGCCCTATGCCGTGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGCGCGCCGCGCAAGGTACTGGCGTGa                                                                                                                                                                   >  2:171257/1‑249 (MQ=255)                                                                                     ggAGAAAAATACGCTGCGCCCGGAGATGAGCAGCGACGAAATTGAGCGCATTATTCGTAGTGCGGTCAATAACGTGCCCTATGCCGTGGGGAGCACCAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCGTTACTTCCTCGACAGCGGAACCATCGGTAATACCCAGGCGATGAGCGCCGCGCTAGGTACTGGCGTGaattggatca                                                                                                                                                          <  2:385750/251‑9 (MQ=255)                                                                                       agaAAAATACGCTGCGCCCGGAGATGAGCAGCGACGAAATTGAGCGCATTATTCGTAGTGCGGTCAATAACGTGCCCTATGCCGTGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGCGCGCCGCGCAAGGTACTGGCGTGAAGGTGATCaa                                                                                                                                                         >  1:55652/1‑250 (MQ=255)                                                                                        gaAAAATACGCTGCGCCCGGAGATGAGCAGCGACGAAATTGAGCGCATTATTCGTAGTGCGGTCAATAACGTGCCCTATGCCGTGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGCGCGCCGCGCAAGGTACTGGCGTGAAGGTGATCAAAc                                                                                                                                                       <  2:335228/251‑1 (MQ=255)                                                                                          aaaaTACGCTGCGCCCGGAGATGAGCAGCGACGAAATTGAGCGCATTATTCGTAGTGCGGTCAATAACGTGCCCTATGCCGTGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGCGCGCCGCGCAAGGTACTGGCGTGAAGGTGATCAAACgg                                                                                                                                                     <  2:63326/251‑1 (MQ=255)                                                                                             aTACGCTGCGCCCGGAGATGAGCAGCGACGAAATTGAGCGCATTATTCGTAGTGCGGTCAATAACGTGCCCTATGCCGTGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGCGCGCCGCGCAAGGTACTGGCGTGAAGGTGATCAAACGGAAg                                                                                                                                                  <  1:274655/251‑1 (MQ=255)                                                                                             aTACGCTGCGCACGGCGATGAGCAGCGACGAAATTGCGCGCATTATTCGTAGTGCGGTCAATAACGTGCCCTATGCCGTGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGCGCGCCGCGCAAGGTACTGGCGTGACGGTGATCAAACGGa                                                                                                                                                    >  2:275531/1‑249 (MQ=255)                                                                                                cgctgcgcCCGGAGATGAGCAGCGACGAAATTGAGCGCATTATTCGTAGTGCGGTCAATAACGTGCCCTATGCCGTGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGCGCGCCGCGCAAGGTACTGGCGTGAAGGTGATCAAACGGAAGGtg                                                                                                                                               >  2:82789/1‑250 (MQ=255)                                                                                                  ctgcgcCCGGAGATGAGCAGCGACGAAATTGAGCGCATTATTCGTAGTGCGGTCAATAACGTGCCCTATGCCGGGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTACCCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGCGCGCCGCGCAAGGTAATGGCGTGAAGGTGATCACACGGCAggggtt                                                                                                                                             >  2:11314/4‑247 (MQ=255)                                                                                                     cgcCCGGCGATGAGAAGCGACGAAATTGAGCTCATTATTCGTAGTGCGGTCAATAACGTGCCCTATGCCGGGGGGATCAACAACCACAGTGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATGCCCAGGCGATGCGCGCCGCGCAAGGTACGGGCGTGAAGGTTATCAACCGGAAGTTGTTCCt                                                                                                                                          <  2:210554/251‑1 (MQ=255)                                                                                                     cgcCCGGAGATGAGCAGCGACGAAATTGAGCGCATTATTCGTAGTGCGGTCAATAACGTGCCCTATGCCGTGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGCGCGCCGCGCAAGGTACTGGCGTGAAGGTGATCAAACGGAAGGTGTTCCt                                                                                                                                          >  2:312149/1‑251 (MQ=255)                                                                                                              attaggagcGGCGAAATTGAGCGCATTATTCGTAGTGCGGTCAATAACGTGCCCTATGCCGGGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGCGCGCCGCGCAAGGTACTGGCGTGAAGGTGATCAAACGGAAGGTGTTCCTCGACGATTc                                                                                                                                 <  2:384996/245‑1 (MQ=255)                                                                                                                gAGCAGCGACGAAATTGAGCGCATTATTCGTAGTGCGGTCAATAACGTGCCCTATGCCGTGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGCGCGCCGCGCAAGGTACTGGCGTGAAGGTGATCAAACGGAAGGTGTTCCTCGACGATTCGc                                                                                                                               <  1:312149/251‑1 (MQ=255)                                                                                                                        acgaAATTGAGCGCATTATTCGTAGTGCGGTCAATAACGTGCCCTATGCCGTGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGCGCGCCGCGCAAGGTACTGGCGTGAAGGTGATCAAACGGAAGGTGTTCCTCGACGATTCGCagaacgaa                                                                                                                       <  1:171257/251‑8 (MQ=255)                                                                                                                                gAGCGCATTATTCGTAGTGCGGTCAATAACGTGCCCTATGCCGTGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGCGCGCCGCGCAAGGTACTGGCGTGAAGGTGATCAAACGGAAGGTGTTCCTCGACGATTCGCAGAACGAAGCGGACAt                                                                                                               <  1:357701/251‑1 (MQ=255)                                                                                                                                   cgcATTATTCGTAGTGCGGTCAATAACGTGCCCTATGCCGTGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGCGCGCCGCGCAAGGTACTGGCGTGAAGGTGATCAAACGGAAGGTGTTCCTCGACGATTCGCAGAACGAAGCGGACATCCg                                                                                                            <  1:16452/251‑1 (MQ=255)                                                                                                                                      attattCGTAGTGCGGTCAATAACGTGCCCTATGCCGTGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGCGCGCCGCGCAAGGTACTGGCGTGAAGGTGATCAAACGGAAGGTGTTCCTCGACGATTCGCAGAACGAAGCGGACATCCgtgt                                                                                                         <  1:152713/251‑1 (MQ=255)                                                                                                                                            cGTAGTGCGGTCAATAACGTGCCCTATGCCGTGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTAGCCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGCGCGCCGCGCAAGGTACTGGCGTGAAGGTGATCAAACGGAAGGTGTTCCTCGACGATTCGCAGAACGAAGCGGACATCCGTGTGCAAt                                                                                                    <  1:52138/250‑1 (MQ=255)                                                                                                                                            cGTAGTGCGGTCAATAACGTGCCCTATGCCGTGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTGACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGCGCGCCGCGCAAGGTACTGGCGTGAAGGTGATCAAACGGAAGGTGTTCCTCGACGATTCGCAGAACGAAGCGGACATCCGTGTGCAAtt                                                                                                   <  1:275531/251‑1 (MQ=255)                                                                                                                                             gTAGTGCGGTCAATAACGTGCACTATGCCGTGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGCGCGCCGCGCAAGGTACTGGCGTGAAGGTGATCAAACGGAAGGTGTTCCTCGACGATTCGCAGAACGAAGCGGACATCCGGGGGCAAttt                                                                                                  >  2:258657/1‑251 (MQ=255)                                                                                                                                               agtgcgagcAATAACGTGCCCTATGCCGTGGGGATCAACAGCCACAGGTGTAGCAAGAGGACCTCTAACCTGTGTGGTATGCATAAAGTGATACAGGCACTAGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGCGCGCCGCGCAAGGTGCTGGCGTGAAGGTGATCAAACGGAAGGTGTTCCTCGACGATTCGCAGATCGAAGCGGACCTCCGTGTTCAATTTaa                                                                                                <  2:96977/243‑1 (MQ=255)                                                                                                                                                gTGCGGTCAATAACGTGCCCTATGCCGTGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGCGCGCCGCGCAAGGTACTGGCGTGAAGGTGATCAAACGGAAGGTGTTCCTCGACGATTCGCAGAAAGAAGAGGACATCAGTGTGCAATTTAAt                                                                                               >  1:364967/1‑251 (MQ=255)                                                                                                                                                       cAATAATGTGCCCTATGCCGTGGGGATAAACAAACACATGGGTCGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAAGGCCCAGTCGATGCGCGCCGCGCAAGGTACTGGCGTTAAGGTGATTAATCGGAAGTTGTTCGTCGACTATTCGCAGAACGAAGCTGACAGCCGTGTGCAATTTAATCTCGCaa                                                                                        <  2:437333/251‑1 (MQ=255)                                                                                                                                                              gTGCCCTATGCCGTGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGCGCGCCGCGCAAGGTACTGGCGTGAAGGTGATCAAACGGAAGGTGTTCCTCGACGATTCGCAGAACGAAGCGGACATCCGTGTGCAATTTAATCGCGCAATTGatct                                                                                 <  1:258657/251‑4 (MQ=255)                                                                                                                                                                 cccTATGCCGTGGGGATCAACAACCACATGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGCGCGCCGCGCAAGGTACTGGCGTGAAGGTGATCAAACGGAAGGTGTTCCTCGACGATTCGCAGAACGAAGCGGACATCCGTGTGCAATTTAATCGCGCAATTGATCTGgc                                                                              >  2:216387/1‑251 (MQ=255)                                                                                                                                                                                    acaacCACAAGGGTAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGCGCGCCGCGCAAGGTACTGGCGTGAAGGTGATCAAACGGAAGGTGTTCCTCGACGATTCGCAGAACGAAGCGGACATCCGTGTGCAATTTAATCGCGCAATTGCTCTGGCGCGGCGCAACGGTTCAAcc                                                           >  2:132737/1‑251 (MQ=255)                                                                                                                                                                                              gggtagcaAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGCGCGCCGCGCAAGGTACTGGCGTGAAGGTGATCAAACGGAAGGTGTTCCTCGACGATTCGCAGAACGAAGCGGACATCCGTGTGCAATTTAATCGCGCAATTGATCTGGCGCGTCGCAACGGTTCAAccattgccattg                                                 <  2:106921/247‑1 (MQ=255)                                                                                                                                                                                                  agcaAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGCGCGCCGCGCAAGGTACTGGCGTGAAGGTGATCAAACGGAAGGTGTTCCTCGACGATTCGCAGAACGAAGCGGACATCCGTGTGCAATTTAATCGCGCAATTGATCTGGCGCGTCGCAACGGTTCAACCATTGCCATTGGGCa                                             <  2:45050/251‑1 (MQ=255)                                                                                                                                                                                                     aaGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGCGCGCCGCGCAAGGTACTGGCGTGAAGGTGATCAAACGGAAGGTGTTCCTCGACGATTCGCAGAACGAAGCGGACATCCGTGTGCAATTTAATCGCGCAATTGATCTGGCGCGTCGCAACGGTTCAACCATTGCCATTGGGCATcc                                          >  2:277768/1‑251 (MQ=255)                                                                                                                                                                                                          gACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGCGCGCCGCGCAAGGTACTGGCGTGAAGGTGATCAAACGGAAGGTGTTCCTCGACGAtt                                                                                                                                  <  1:149463/158‑1 (MQ=255)                                                                                                                                                                                                          gACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGCGCGCCGCGCAAGGTACTGGCGTGAAGGTGATCAAACGGAAGGTGTTCCTCGACGAtt                                                                                                                                  >  2:149463/1‑158 (MQ=255)                                                                                                                                                                                                                 aaCCTGTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCTGTAATACCCAGGCGATGCGCGCCGCGCAAGGTACTGGCGTGAAGGTGATCAAACGGAAGGTGTTCCTCGACGATTCGCAGAACGAAGCGGACATCCGTGTGCAATTTAATCGCGCAATTGATCTGGCGCGTCGCAACGGTTCAACCATTGCCATTGGGCATCCTCACCCTTCAAc                              <  1:250386/251‑1 (MQ=255)                                                                                                                                                                                                                      gTTTGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGCGCGCCGCGCAAGGTACTTGCGTGAAGGTGATCAAACGGAAGGTGTTCCTCGACGATTCGCAGAACGAAGCGGACATCCGTGTGCAATTTAATCGCGCAATTGATCTGGCGCGTCGCAACGGTTCAACCATTGCCATTGGGCATCCTCACCCTTCAACGGTAc                         <  1:366287/251‑1 (MQ=255)                                                                                                                                                                                                                        ttGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGCGCGCCGCGCAAGGTACTGGCGTGAAGGTGATCAAACGGAAGGTGTTCCTCGACGATTCGCAGAACGAAGCGGACATCCGTGTGCAATTTAATCGCGCAATTGATCTGGCGCGTCGCAACGGTTCAACCATTGCCATTGGGCATCCTCACCCTTCAACGGTAcg                        <  2:55652/250‑1 (MQ=255)                                                                                                                                                                                                                         tGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGCGCGCCGCGCAAGGTACTGGCGTGAAGGTGATCAAACGGAAGGTGTTCCTCGACGATTCGCAGAACGAAGCGGAc                                                                                                                 >  1:270639/1‑160 (MQ=255)                                                                                                                                                                                                                         tGGTATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGCGCGCCGCGCAAGGTACTGGCGTGAAGGTGATCAAACGGAAGGTGTTCCTCGACGATTCGCAGAACGAAGCGGAc                                                                                                                 <  2:270639/160‑1 (MQ=255)                                                                                                                                                                                                                            tATGCAGAAAGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGCGCGCCGCGCAAGGTACTGGCGTGAAGGTGATCAAACGGAAGGTGTTCCTCGACGATTCGCAGAACGAAGCGGACATCCGTGGGCAATTTAATCGCGCAATTGATCTGGCGCGTCGCAACGGTTCAACCATTGCCATTGGGCATCCTCACCCTTCAACGGTACGCgtgc                   >  2:129777/1‑250 (MQ=255)                                                                                                                                                                                                                               gCAGAAAGTGAGTCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCATAACCATCGGTAATACCCAGGCGAGGCGCGCCTCGCAAGGTACTGGTGTGAAGGTGATCAAACGGAAGGTGTTCCGCGACGATTCGCAGAACGAAGCGGACAACCGTGTGCAATTTAATCGCGCAATTGATCTGGCTCGACGCAACGGTTCAACCATTGCTATTGGGCATCTTCACCCTTCAACGGTACGCgtgcttc                <  2:290352/251‑5 (MQ=255)                                                                                                                                                                                                                                   aaaGTGATGCAGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGCGCGCCGCGCAAGGTACTGGCGTGAAGGTGATCAAACGGAAGGTGTTCCTCGACGATTCGCAGAACGAAGCGGACATCCGTGTGCAATTTAATCGCGCCATTGATCTGGCGCGTCGCAACGGTTCAACCATTGCCATTGGGCATCCTCACCCTTCAACGGTACGCgtgctacaac             >  1:411961/1‑243 (MQ=255)                                                                                                                                                                                                                                             aGGCACTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGCGCGCCGCGCAAGGTACTGGCGTGAAGGTGATCAAACGGAAGGTGTTCCTCGACGATTCGCAGAACGAAGCGGACATCCGTGTGCAATTTAATCGCGCAATTGATCTGGCGCGTCGCAACGGTTCAACCATTGCCATTGGGCATCCTCACCCTTCAACGGTACGCGTGCTACAACAGATGGTTtat  >  1:151309/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                       CAGAGAGATGGCGACCAAAGCGCATAACAGCGGGCACGAAGTGTTGATTCATCTCCCGATGGCACCGTTGAGTAAACAGCCGCTGGAGAAAAATACGCTACGCCCGGAGATGAGCAGCGACGAAATTGAGCGCATTATTCGTAGTGCGGTCAATAACGTGCCCTATGCCGTGGGGATCAACAACCACATGGGCAGCAAGATGACCTCTAACCTGTTTGGTATGCAGAAAGTGATGCAGGCGCTGGAGCGTTACAATCTTTACTTCCTCGACAGCGTAACCATCGGTAATACCCAGGCGATGCGCGCCGCGCAAGGCACTGGCGTGAAGGTGATCAAACGGAAGGTATTCCTCGACGATTCGCAAAATGAAGCGGACATCCGTGTGCAATTTAATCGCGCAATTGACCTGGCGCGTCGCAACGGTTCGACCATTGCCATTGGGCATCCTCACCCTGCAACGGTACGCGTGTTGCAACAGATGGTTTAT  >  NC_000913/3788283‑3788769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑