Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,787,809	T→G	V324V (GTT→GTG)	envC →	murein hydrolase activator EnvC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,787,809	0	T	G	98.8%	273.4 / NA	80	V324V (GTT→GTG)	envC	murein hydrolase activator EnvC
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (37/42);  total (38/42)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCC  >  NC_000913/3787577‑3788053                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       tccgatctcgTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTAGAGACCGCCAGAAAGAAGCGAAGAGCAAAGGCACCACCTACACACAGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGGGGCCTGGGTGCGCCGCGTGGTCCGGCATTCTGGCCGGTTCGCGGGCCGACGTTGCATCGCTATGGCGAGCAACTACCGGGTGAATTAAGCTGGAGAGGGATGGTGATTGGTGCCTCTGAAGGt                                                                                                                                                                                                                                      >  1:172230/9‑251 (MQ=255) cgAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGGTGGTGATTGGTGCCTCTGAAggg                                                                                                                                                                                                                                      >  2:328758/1‑250 (MQ=255)  gAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGCTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCCCGGGCCGACGCCGCATCGCTATGGCGAGCAGCTACAGGGCGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAgga                                                                                                                                                                                                                                      >  1:120179/1‑249 (MQ=255)   aaaGCGCGTGCAGAACGAGAAGCACGGGAGGCCCAGGCGGCTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGGGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGGCGCTGCATCGCTATGGCTAGCAGCTACAGGGTGAAGTACGCTGGAAAGGGATGGTGATTGGTTCCTCTGAGGGTAc                                                                                                                                                                                                                                    <  2:409280/251‑1 (MQ=255)    aaGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACAGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGAGCCGCGGGGTCAGGCATTCTGGCCGGTTCGCGGGCCGTCTCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGGAAGGGGTGGGGATTGGTGCCTCTGAAGGTAC‑t                                                                                                                                                                                                                                  >  1:436097/1‑251 (MQ=255)        gcgTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAg                                                                                                                                                                                                                              <  1:227870/251‑1 (MQ=255)        gcgTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGGGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCGGCATCGCTATGGCGGGCAGCTAAAGGGGGAATTACGCTGGAAAGGGATGGGGATTGGTGCCTCTGAAGGTAC‑TGa                                                                                                                                                                                                                                >  2:21103/1‑249 (MQ=255)              aGAACGAGAATCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAg                                                                                                                                                                                                                        <  1:194926/251‑1 (MQ=255)              aGAACGAGAAGAACGTGAGGCCCAGGCGGTACGAGCCCCACAGAAAGAAGCGACGCGCCAACGCCCCAACTACAAACCGACCGCAAGCGAAAAATAGCTGGTGTCCCGAACTGGTGGCCTGGGAGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGGGAGCAGAGAAAGGGTGAATTAAGCTGGGAAGGGCTGGTGATTGGGGCCTCTGAAGGTAC‑TTAAGTTaaa                                                                                                                                                                                                                         >  2:392609/1‑250 (MQ=255)                aaCGAGAAGCACGGGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCg                                                                                                                                                                                                                      <  2:318573/251‑1 (MQ=255)                      atgCACGTGAGGCCCAGGCGGTTCGCAACCGCCAGAAAGAAGCGACGCGCAAAGGCACCCCCTACAACCCTACCGAAAGCGAAAATTCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCTCTCTGGCCGGTTCGCGGGCCGTCGCTGCATCGTTATGGCGAGCAGCTACAGGGTGAATTACGCGGTAAAGGGATGGTGATTGGTGCCGCTGAAGGTAC‑TGAAGTTAAAGCGATTGcc                                                                                                                                                                                                                <  1:254851/249‑1 (MQ=255)                       aGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCCCCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGGAC‑TGAAGTTAAAGCGATTGCCg                                                                                                                                                                                                               >  1:343759/1‑251 (MQ=255)                       aGCACGGGAGGCCCAGGCGGTGCGCGACCTCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAATCGACCGAAAGCGAAAAAGCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGctg                                                                                                                                                                                                               <  2:23367/251‑3 (MQ=255)                        gCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGGGATTGGTGCCTCTGAAGGGAC‑TGAAGTTAAAGCGATTGCCg                                                                                                                                                                                                               >  2:278334/1‑250 (MQ=255)                             ggAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTc                                                                                                                                                                                                         <  2:413677/250‑1 (MQ=255)                                atcccAGGCGGTTCGTGACCGCCAAAAAAAACCGACGCTCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCAGGGTCGCGGGCCGACGCTGCATCGCTATGGCGATCAGCTACAGGGTTAATTACGCTGGAAAGGGATGGTGATTGGTGTCTCTGAAGGTAC‑TGAAGTTAAAGCGATGGCCGACGGTCggg                                                                                                                                                                                                      <  2:309112/249‑1 (MQ=255)                                            gcgcgACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATGCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTg                                                                                                                                                                                          <  2:82437/250‑1 (MQ=255)                                             cgcgACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTg                                                                                                                                                                                          <  1:108961/250‑1 (MQ=255)                                             cgcgACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACGGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGAGTCTGGCTg                                                                                                                                                                                          <  2:313419/250‑1 (MQ=255)                                                 aCCGCCAGAAAGAAGCGACGCGCACAGGCACCAACTACAAACCGACCGAAAGCGAAAAATAGCTGATGTCCCGAACTGGTGGCATGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGCTGGGGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTATGGCTGACTgg                                                                                                                                                                                     >  2:423312/1‑251 (MQ=255)                                                 aCCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTgg                                                                                                                                                                                     >  1:361927/1‑251 (MQ=255)                                                     ccAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGc                                                                                                                                                                                 <  1:10733/251‑1 (MQ=255)                                                      cAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGACGACGGTCGGGTGATTCTGGCTGACTGGCTGc                                                                                                                                                                                 >  1:296320/1‑250 (MQ=255)                                                        gaaagaaGCGACGCGCAAAGGCACCACCTCCAAACCGACCGAAAGCGACAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGACTTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCa                                                                                                                                                                                >  2:425173/1‑249 (MQ=255)                                                            tccgatctgcgcAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCt                                                                                                                                                                           >  1:113062/9‑250 (MQ=255)                                                             aagCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTGC‑TGAAGTGAAAGCGATTGCCGACGGTCGGGTGAGTCTGGCTGACTGGCTGCAAGGCTAc                                                                                                                                                                         <  2:154021/251‑1 (MQ=255)                                                                   cgcgCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCtg                                                                                                                                                                   <  1:274861/251‑1 (MQ=255)                                                                        aaaGGCACCACCTACAAACCGCCCGCAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGAGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTAAAGGGTGAATTACGCTGGAAAGGGCTGGTGATTGGTGCCTCTGCCGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCtggcggg                                                                                                                                                              >  2:424194/1‑247 (MQ=255)                                                                        aaaGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGAGATTCTGGCTGACTGGCTGCAAGGCTACGGTCtggtggg                                                                                                                                                              >  1:347929/1‑250 (MQ=255)                                                                           ggCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGACAGGCATTCTGGCCTGTTCGCGGGCCGACGCGGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCtggtggtggt                                                                                                                                                           <  2:100093/251‑1 (MQ=255)                                                                           ggCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACGGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCtggtggtggt                                                                                                                                                           <  1:425173/251‑1 (MQ=255)                                                                              accaccTACAAACCGACCGACAGCGAAAAATCGATGACGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGAAGCTGCATCGCTATGGCGAGTAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCAGACGTTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCtggtggtggtggg                                                                                                                                                        >  2:305710/1‑250 (MQ=255)                                                                               ccaccTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑tt                                                                                                                                                      <  1:89589/251‑1 (MQ=255)                                                                                caccTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGGGGTGG‑TTg                                                                                                                                                     >  2:17042/1‑251 (MQ=255)                                                                                 accTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTg                                                                                                                                                     >  1:232115/1‑250 (MQ=255)                                                                                      cAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGCACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATg                                                                                                                                               >  2:330909/1‑251 (MQ=255)                                                                                      cAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATg                                                                                                                                               >  1:5848/1‑251 (MQ=255)                                                                                       aaaCCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATgg                                                                                                                                              <  1:154654/251‑1 (MQ=255)                                                                                        aacagcccgaAAGCGAAAAATCGCTGATGTCACGAACTGGTGACCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGCGCAGCTACAGGGTGAATTACGCTGGACAGGGATGGTGATTGGTGCCTCTGAAGGTCC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGt                                                                                                                                             >  2:163849/7‑251 (MQ=255)                                                                                          ccgaccgaAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTaa                                                                                                                                           <  2:377311/251‑1 (MQ=255)                                                                                            gaccgaAAGCGAAAAATAGCTGATGTCCCGAACTGGAGGCATGGGTGCGTCGAGTGGACAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGCGCAGCTACAGGGTGACTGACGCTGGAATGGGATGGTTATTGGTGCCTCTGACGG‑ACGTGAAGTTACAACGATTGCCGCCGGCCGGTTGATTCTGGCTGACTGGGTGAAAGGCGACGGTCTGGTGG‑GGTGGGATGCGCATGGTaa                                                                                                                                           >  2:14222/1‑249 (MQ=255)                                                                                             accgaAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAgg                                                                                                                                        <  1:340561/251‑1 (MQ=255)                                                                                                gaAAGCGAAAAATCGCGGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGa                                                                                                                                     <  2:45545/251‑1 (MQ=255)                                                                                                 aaaGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCGGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGAc                                                                                                                                    <  1:313958/251‑1 (MQ=255)                                                                                                  aagggAAAAATCTCTGATGTCGCGAACTTGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGACGGTGCGCGGGCCGACGCTGCATGGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACa                                                                                                                                   <  2:74732/247‑1 (MQ=255)                                                                                                  aaGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACa                                                                                                                                   <  1:252267/251‑1 (MQ=255)                                                                                                      gAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATg                                                                                                                                 >  1:30668/1‑249 (MQ=255)                                                                                                       aaaaaTCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGc                                                                                                                                       <  1:339441/242‑1 (MQ=255)                                                                                                       aaaaaTCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGGGGTGGTGG‑TTGAGCCTGGTAAAGGc                                                                                                                                       >  2:339441/1‑242 (MQ=255)                                                                                                        aaaaTCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTc                                                                                                                             >  2:318907/1‑251 (MQ=255)                                                                                                                tGATGTCCCGAACTGGTGGCATGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGACAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAACGGCGACATGAGTCTTTACGGc                                                                                                                     >  1:437333/1‑251 (MQ=255)                                                                                                                 gATGTCCCTAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGTTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACgg                                                                                                                      <  1:255106/249‑1 (MQ=255)                                                                                                                       ccgaactggtggcCTGGGTGCGCCGCTTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATc                                                                                                              <  1:418400/244‑1 (MQ=255)                                                                                                                        cgaactggtggcCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATc                                                                                                              >  1:197653/7‑250 (MQ=255)                                                                                                                                 ggccTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACt                                                                                                    >  1:387267/4‑251 (MQ=255)                                                                                                                                   ccTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGACGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGGGGTGG‑GTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTgg                                                                                                  >  2:278424/2‑251 (MQ=255)                                                                                                                                        gtgcgcCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTAAAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATGCTGGATGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGc                                                                                             <  2:167138/249‑1 (MQ=255)                                                                                                                                            gccgcGTGGTCCGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGGGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTg                                                                                         >  2:370828/1‑251 (MQ=255)                                                                                                                                                    gTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAg                                                                                 <  2:113062/251‑1 (MQ=255)                                                                                                                                                    gTCAGGCATGCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAg                                                                                 <  1:91038/251‑1 (MQ=255)                                                                                                                                                       aGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGtt                                                                              <  1:401936/251‑1 (MQ=255)                                                                                                                                                        ggCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCACTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTGACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTc                                                                             <  1:424194/251‑1 (MQ=255)                                                                                                                                                                 gCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACCGGGTGAATTACGCTGGAAAGGGATGGTGCTTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTATGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGGGCATGGTAAGGGCGACATGCGTCTTTACGGCTATAATCAGCGCGCACTGGTGAGCGTTGGTTCGCCGGTTCGCGCGGGcc                                                                    >  2:421330/1‑251 (MQ=255)                                                                                                                                                                    ggTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGc                                                                 <  1:118652/251‑1 (MQ=255)                                                                                                                                                                          cgGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTg                                                           <  2:347929/251‑1 (MQ=255)                                                                                                                                                                          cgGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTg                                                           >  1:235223/1‑251 (MQ=255)                                                                                                                                                                                 aCGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCACtggt                                                    <  1:118173/251‑1 (MQ=255)                                                                                                                                                                                   gCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTGCGCAGGTTCGCGCGGGCCAGCCAATTGCACtggtgg                                                  <  2:197653/251‑1 (MQ=255)                                                                                                                                                                                      gCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCACTGGTGGgc                                                <  2:233355/250‑1 (MQ=255)                                                                                                                                                                                      gCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCGGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCACTGGTGGgc                                                <  1:353806/250‑1 (MQ=255)                                                                                                                                                                                        aTCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGGGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCACTGGTGGgcagc                                             >  2:140247/1‑251 (MQ=255)                                                                                                                                                                                          cGCTAGGGCGAGCAGCTACAGCGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGGGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGGTGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTGTACTGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGTGCCAGCCAATTGCACTGGTGGGcagcag                                           <  2:436097/251‑1 (MQ=255)                                                                                                                                                                                           ccTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATTAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCACTGGTGGGCAGCAGt                                          <  2:378521/250‑1 (MQ=255)                                                                                                                                                                                             tATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCACTGGTGGGCAGCAGTgg                                        >  1:204391/1‑251 (MQ=255)                                                                                                                                                                                                       aGCTACTGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCAACGGTCTGGTGGTGGTGG‑TTGCGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGGGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCACGGGCCAGCCACTTGCACTGGTGGGCAGCAGGGGCggaccaggg                              >  2:86124/1‑244 (MQ=255)                                                                                                                                                                                                          tACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCACTTGCACTGGTGGGCAGCAGTGGCGGTccagggcgg                           >  1:12537/1‑243 (MQ=255)                                                                                                                                                                                                             aGGGTGAAGTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCt                        <  1:370828/251‑1 (MQ=255)                                                                                                                                                                                                                   aaTTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGGCGGccctccctc                  >  1:171976/1‑244 (MQ=255)                                                                                                                                                                                                                                  ggaTGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCACTGGTGGGCAGCAGTGGCGGTCTGGGTCGGCCTTCACTCTATTTTGGAATTcgc   >  1:296613/3‑251 (MQ=255)                                                                                                                                                                                                                                   gaTGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTcgcc  <  2:5848/250‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                       CGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGGCGGTCAGGGTCGGCCTTCACTCTATTTCGAAATTCGCC  >  NC_000913/3787577‑3788053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑