Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,787,773	A→G	E312E (GAA→GAG)	envC →	murein hydrolase activator EnvC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,787,773	0	A	G	98.9%	287.2 / NA	92	E312E (GAA→GAG)	envC	murein hydrolase activator EnvC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (45/46);  minor base T (0/1);  total (45/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

AACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGG  >  NC_000913/3787531‑3788015                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     aaCGAATCCCGTCTGCGGAAAAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCCCGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGACTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGACGCCGCTGCCTCGCTATGGCGAGCAgatcc                                                                                                                                                                                                                                               >  1:379864/1‑246 (MQ=255)        cccGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGGCGACGCTGCATCGCTATGGCGAGCAGCTACCggggga                                                                                                                                                                                                                                        >  1:119506/1‑247 (MQ=255)          cGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAAtt                                                                                                                                                                                                                                     <  1:79836/251‑1 (MQ=255)          cGTCAGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAAtt                                                                                                                                                                                                                                     <  2:313201/251‑1 (MQ=255)           ggcTGCGTAACAGGATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAAAACGAGAAGCACGGGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCTACACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACGGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTa                                                                                                                                                                                                                                    <  1:299451/249‑1 (MQ=255)           gTCTGCGTAACAGCATTGCCCGTGCGGAAGACGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTACCGAACTGGGGGCCTGGTGGCACCGCGTGATTAGGCATTCTGGCAGGTTCACGGGCCAACGCTGCATCGCTATGGCGAGCAGCGACAGGGTGAAtg                                                                                                                                                                                                                                     >  1:371701/1‑249 (MQ=255)            tCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGCGCAGCTACAGGGTGGATTAc                                                                                                                                                                                                                                   >  2:118652/1‑251 (MQ=255)                   aaCAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCCAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGAGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTggg                                                                                                                                                                                                                             >  1:223025/1‑249 (MQ=255)                     cAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGCCGCGCAAAGGCACCCCCTACAAACCGACCGAAAGCGAACAATCGCTGATGTCCCGAACTGGTGGCCTGGGGGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTGCGCTGGaa                                                                                                                                                                                                                            >  2:340561/1‑249 (MQ=255)                      aGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCCAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGAGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTAGGGCGCGCAGCTACAGGGAGAATTACGCTGGAAAgg                                                                                                                                                                                                                         >  2:425942/1‑251 (MQ=255)                       gCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAggg                                                                                                                                                                                                                        >  1:233355/1‑250 (MQ=255)                           cggccgggCGGAAGCCGCGGCGAAAGCGAGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGAGAACGCCAGAAAGAAGCGACGCGCAAAGGCCACACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCCGGCATTCTGGCCGGTTCGCGGGACGAGGCTGCATCGCTATGGCGAGGAGCTACAGGGTGAATTACGCTGGAAAGGGATg                                                                                                                                                                                                                     <  2:101944/243‑1 (MQ=255)                            gCCCGTGCGGACGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACCAACCGAACGAAAGCGAAAAATCGCTGATGTCCCGCACTGGGCGCCTGGGGGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCGGCAGCCGCATCGCTATGGCGAGCAGGTACCGGGGGGATTACGCTGGaaaaggagggg                                                                                                                                                                                                                   >  2:229926/1‑243 (MQ=255)                            gCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGt                                                                                                                                                                                                                   <  1:342448/251‑1 (MQ=255)                                gTGCGGAAGCCGCGGCGCAAGCGCGTGCAGAACGTGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCCACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGATCGAGGGCCGACGGTGAATCGCTATGGCGTGCAACTAAAGGGTGAATTACGCGGGCAAggggggggggtt                                                                                                                                                                                                               >  2:352038/1‑241 (MQ=255)                                 ggCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGtgattg                                                                                                                                                                                                              <  1:169004/250‑6 (MQ=255)                                     gAAGCCGCGGCGAAAGCGCGTGCAGAACGGGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATggggattggtgc                                                                                                                                                                                                          >  1:313419/1‑241 (MQ=255)                                         ccGCGGCGAAAGCGCGTGACGAACGAGAAGCGCGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGtgattgttgcctct                                                                                                                                                                                                      <  2:226925/251‑14 (MQ=255)                                               tccgatctcgTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTAGAGACCGCCAGAAAGAAGCGAAGAGCAAAGGCACCACCTACACACAGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGGGGCCTGGGTGCGCCGCGTGGTCCGGCATTCTGGCCGGTTCGCGGGCCGACGTTGCATCGCTATGGCGAGCAACTACCGGGTGAATTAAGCTGGAGAGGGATGGTGATTGGTGCCTCTGAAGGt                                                                                                                                                                                                >  1:172230/9‑251 (MQ=255)                                               cgAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGGTGGTGATTGGTGCCTCTGAAggg                                                                                                                                                                                                >  2:328758/1‑250 (MQ=255)                                                gAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGCTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCCCGGGCCGACGCCGCATCGCTATGGCGAGCAGCTACAGGGCGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAgga                                                                                                                                                                                                >  1:120179/1‑249 (MQ=255)                                                 aaaGCGCGTGCAGAACGAGAAGCACGGGAGGCCCAGGCGGCTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGGGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGGCGCTGCATCGCTATGGCTAGCAGCTACAGGGTGAAGTACGCTGGAAAGGGATGGTGATTGGTTCCTCTGAGGGTAc                                                                                                                                                                                              <  2:409280/251‑1 (MQ=255)                                                  aaGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACAGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGAGCCGCGGGGTCAGGCATTCTGGCCGGTTCGCGGGCCGTCTCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGGAAGGGGTGGGGATTGGTGCCTCTGAAGGTAC‑t                                                                                                                                                                                            >  1:436097/1‑251 (MQ=255)                                                      gcgTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAg                                                                                                                                                                                        <  1:227870/251‑1 (MQ=255)                                                      gcgTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGGGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCGGCATCGCTATGGCGGGCAGCTAAAGGGGGAATTACGCTGGAAAGGGATGGGGATTGGTGCCTCTGAAGGTAC‑TGa                                                                                                                                                                                          >  2:21103/1‑249 (MQ=255)                                                            aGAACGAGAATCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAg                                                                                                                                                                                  <  1:194926/251‑1 (MQ=255)                                                            aGAACGAGAAGAACGTGAGGCCCAGGCGGTACGAGCCCCACAGAAAGAAGCGACGCGCCAACGCCCCAACTACAAACCGACCGCAAGCGAAAAATAGCTGGTGTCCCGAACTGGTGGCCTGGGAGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGGGAGCAGAGAAAGGGTGAATTAAGCTGGGAAGGGCTGGTGATTGGGGCCTCTGAAGGTAC‑TTAAGTTaaa                                                                                                                                                                                   >  2:392609/1‑250 (MQ=255)                                                              aaCGAGAAGCACGGGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCg                                                                                                                                                                                <  2:318573/251‑1 (MQ=255)                                                                    atgCACGTGAGGCCCAGGCGGTTCGCAACCGCCAGAAAGAAGCGACGCGCAAAGGCACCCCCTACAACCCTACCGAAAGCGAAAATTCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCTCTCTGGCCGGTTCGCGGGCCGTCGCTGCATCGTTATGGCGAGCAGCTACAGGGTGAATTACGCGGTAAAGGGATGGTGATTGGTGCCGCTGAAGGTAC‑TGAAGTTAAAGCGATTGcc                                                                                                                                                                          <  1:254851/249‑1 (MQ=255)                                                                     aGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCCCCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGGAC‑TGAAGTTAAAGCGATTGCCg                                                                                                                                                                         >  1:343759/1‑251 (MQ=255)                                                                     aGCACGGGAGGCCCAGGCGGTGCGCGACCTCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAATCGACCGAAAGCGAAAAAGCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGctg                                                                                                                                                                         <  2:23367/251‑3 (MQ=255)                                                                      gCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGGGATTGGTGCCTCTGAAGGGAC‑TGAAGTTAAAGCGATTGCCg                                                                                                                                                                         >  2:278334/1‑250 (MQ=255)                                                                           ggAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTc                                                                                                                                                                   <  2:413677/250‑1 (MQ=255)                                                                              atcccAGGCGGTTCGTGACCGCCAAAAAAAACCGACGCTCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCAGGGTCGCGGGCCGACGCTGCATCGCTATGGCGATCAGCTACAGGGTTAATTACGCTGGAAAGGGATGGTGATTGGTGTCTCTGAAGGTAC‑TGAAGTTAAAGCGATGGCCGACGGTCggg                                                                                                                                                                <  2:309112/249‑1 (MQ=255)                                                                                          gcgcgACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATGCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTg                                                                                                                                                    <  2:82437/250‑1 (MQ=255)                                                                                           cgcgACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTg                                                                                                                                                    <  1:108961/250‑1 (MQ=255)                                                                                           cgcgACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACGGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGAGTCTGGCTg                                                                                                                                                    <  2:313419/250‑1 (MQ=255)                                                                                               aCCGCCAGAAAGAAGCGACGCGCACAGGCACCAACTACAAACCGACCGAAAGCGAAAAATAGCTGATGTCCCGAACTGGTGGCATGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGCTGGGGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTATGGCTGACTgg                                                                                                                                               >  2:423312/1‑251 (MQ=255)                                                                                               aCCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTgg                                                                                                                                               >  1:361927/1‑251 (MQ=255)                                                                                                   ccAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGc                                                                                                                                           <  1:10733/251‑1 (MQ=255)                                                                                                    cAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGACGACGGTCGGGTGATTCTGGCTGACTGGCTGc                                                                                                                                           >  1:296320/1‑250 (MQ=255)                                                                                                      gaaagaaGCGACGCGCAAAGGCACCACCTCCAAACCGACCGAAAGCGACAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGACTTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCa                                                                                                                                          >  2:425173/1‑249 (MQ=255)                                                                                                          tccgatctgcgcAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCt                                                                                                                                     >  1:113062/9‑250 (MQ=255)                                                                                                           aagCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTGC‑TGAAGTGAAAGCGATTGCCGACGGTCGGGTGAGTCTGGCTGACTGGCTGCAAGGCTAc                                                                                                                                   <  2:154021/251‑1 (MQ=255)                                                                                                                 cgcgCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCtg                                                                                                                             <  1:274861/251‑1 (MQ=255)                                                                                                                      aaaGGCACCACCTACAAACCGCCCGCAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGAGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTAAAGGGTGAATTACGCTGGAAAGGGCTGGTGATTGGTGCCTCTGCCGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCtggcggg                                                                                                                        >  2:424194/1‑247 (MQ=255)                                                                                                                      aaaGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGAGATTCTGGCTGACTGGCTGCAAGGCTACGGTCtggtggg                                                                                                                        >  1:347929/1‑250 (MQ=255)                                                                                                                         ggCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGACAGGCATTCTGGCCTGTTCGCGGGCCGACGCGGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCtggtggtggt                                                                                                                     <  2:100093/251‑1 (MQ=255)                                                                                                                         ggCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACGGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCtggtggtggt                                                                                                                     <  1:425173/251‑1 (MQ=255)                                                                                                                            accaccTACAAACCGACCGACAGCGAAAAATCGATGACGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGAAGCTGCATCGCTATGGCGAGTAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCAGACGTTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCtggtggtggtggg                                                                                                                  >  2:305710/1‑250 (MQ=255)                                                                                                                             ccaccTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑tt                                                                                                                <  1:89589/251‑1 (MQ=255)                                                                                                                              caccTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGGGGTGG‑TTg                                                                                                               >  2:17042/1‑251 (MQ=255)                                                                                                                               accTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTg                                                                                                               >  1:232115/1‑250 (MQ=255)                                                                                                                                    cAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGCACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATg                                                                                                         >  2:330909/1‑251 (MQ=255)                                                                                                                                    cAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATg                                                                                                         >  1:5848/1‑251 (MQ=255)                                                                                                                                     aaaCCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATgg                                                                                                        <  1:154654/251‑1 (MQ=255)                                                                                                                                      aacagcccgaAAGCGAAAAATCGCTGATGTCACGAACTGGTGACCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGCGCAGCTACAGGGTGAATTACGCTGGACAGGGATGGTGATTGGTGCCTCTGAAGGTCC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGt                                                                                                       >  2:163849/7‑251 (MQ=255)                                                                                                                                        ccgaccgaAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTaa                                                                                                     <  2:377311/251‑1 (MQ=255)                                                                                                                                          gaccgaAAGCGAAAAATAGCTGATGTCCCGAACTGGAGGCATGGGTGCGTCGAGTGGACAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGCGCAGCTACAGGGTGACTGACGCTGGAATGGGATGGTTATTGGTGCCTCTGACGG‑ACGTGAAGTTACAACGATTGCCGCCGGCCGGTTGATTCTGGCTGACTGGGTGAAAGGCGACGGTCTGGTGG‑GGTGGGATGCGCATGGTaa                                                                                                     >  2:14222/1‑249 (MQ=255)                                                                                                                                           accgaAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAgg                                                                                                  <  1:340561/251‑1 (MQ=255)                                                                                                                                              gaAAGCGAAAAATCGCGGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGa                                                                                               <  2:45545/251‑1 (MQ=255)                                                                                                                                               aaaGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCGGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGAc                                                                                              <  1:313958/251‑1 (MQ=255)                                                                                                                                                aagggAAAAATCTCTGATGTCGCGAACTTGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGACGGTGCGCGGGCCGACGCTGCATGGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACa                                                                                             <  2:74732/247‑1 (MQ=255)                                                                                                                                                aaGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACa                                                                                             <  1:252267/251‑1 (MQ=255)                                                                                                                                                    gAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATg                                                                                           >  1:30668/1‑249 (MQ=255)                                                                                                                                                     aaaaaTCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGc                                                                                                 <  1:339441/242‑1 (MQ=255)                                                                                                                                                     aaaaaTCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGGGGTGGTGG‑TTGAGCCTGGTAAAGGc                                                                                                 >  2:339441/1‑242 (MQ=255)                                                                                                                                                      aaaaTCGCTGATGTCCCGAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTc                                                                                       >  2:318907/1‑251 (MQ=255)                                                                                                                                                              tGATGTCCCGAACTGGTGGCATGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGACAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAACGGCGACATGAGTCTTTACGGc                                                                               >  1:437333/1‑251 (MQ=255)                                                                                                                                                               gATGTCCCTAACTGGTGGCCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGTTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACgg                                                                                <  1:255106/249‑1 (MQ=255)                                                                                                                                                                     ccgaactggtggcCTGGGTGCGCCGCTTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATc                                                                        <  1:418400/244‑1 (MQ=255)                                                                                                                                                                      cgaactggtggcCTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATc                                                                        >  1:197653/7‑250 (MQ=255)                                                                                                                                                                               ggccTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACt                                                              >  1:387267/4‑251 (MQ=255)                                                                                                                                                                                 ccTGGGTGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGACGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGGGGTGG‑GTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTgg                                                            >  2:278424/2‑251 (MQ=255)                                                                                                                                                                                      gtgcgcCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTAAAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATGCTGGATGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGc                                                       <  2:167138/249‑1 (MQ=255)                                                                                                                                                                                          gccgcGTGGTCCGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGGGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTg                                                   >  2:370828/1‑251 (MQ=255)                                                                                                                                                                                                  gTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAg                                           <  2:113062/251‑1 (MQ=255)                                                                                                                                                                                                  gTCAGGCATGCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAg                                           <  1:91038/251‑1 (MQ=255)                                                                                                                                                                                                     aGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGtt                                        <  1:401936/251‑1 (MQ=255)                                                                                                                                                                                                      ggCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCACTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTGACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTc                                       <  1:424194/251‑1 (MQ=255)                                                                                                                                                                                                               gCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACCGGGTGAATTACGCTGGAAAGGGATGGTGCTTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTATGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGGGCATGGTAAGGGCGACATGCGTCTTTACGGCTATAATCAGCGCGCACTGGTGAGCGTTGGTTCGCCGGTTCGCGCGGGcc                              >  2:421330/1‑251 (MQ=255)                                                                                                                                                                                                                  ggTTCGCGGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGc                           <  1:118652/251‑1 (MQ=255)                                                                                                                                                                                                                        cgGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTg                     >  1:235223/1‑251 (MQ=255)                                                                                                                                                                                                                        cgGGCCGACGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTg                     <  2:347929/251‑1 (MQ=255)                                                                                                                                                                                                                               aCGCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCACtggt              <  1:118173/251‑1 (MQ=255)                                                                                                                                                                                                                                 gCTGCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTGCGCAGGTTCGCGCGGGCCAGCCAATTGCACtggtgg            <  2:197653/251‑1 (MQ=255)                                                                                                                                                                                                                                    gCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCACTGGTGGgc          <  2:233355/250‑1 (MQ=255)                                                                                                                                                                                                                                    gCATCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCGGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCACTGGTGGgc          <  1:353806/250‑1 (MQ=255)                                                                                                                                                                                                                                      aTCGCTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGGGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCACTGGTGGgcagc       >  2:140247/1‑251 (MQ=255)                                                                                                                                                                                                                                        cGCTAGGGCGAGCAGCTACAGCGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGGGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGGTGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTGTACTGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGTGCCAGCCAATTGCACTGGTGGGcagcag     <  2:436097/251‑1 (MQ=255)                                                                                                                                                                                                                                         ccTATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATTAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCACTGGTGGGCAGCAGt    <  2:378521/250‑1 (MQ=255)                                                                                                                                                                                                                                           tATGGCGAGCAGCTACAGGGTGAATTACGCTGGAAAGGGATGGTGATTGGTGCCTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCAATTGCACTGGTGGGCAGCAGTgg  >  1:204391/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     AACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGGAAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCCGCGTGGTCAGGCATTCTGGCCGGTTCGCGGGCCGACGCTGCATCGCTATGGCGAACAGCTACAGGGTGAATTACGCTGGAAAGGTATGGTTATCGGTGCTTCTGAAGGTAC‑TGAAGTTAAAGCGATTGCCGACGGTCGGGTGATTCTGGCTGACTGGCTGCAAGGCTACGGTCTGGTGGTGGTGG‑TTGAGCATGGTAAAGGCGACATGAGTCTTTACGGCTATAATCAGAGCGCACTGGTGAGCGTTGGTTCGCAGGTTCGCGCGGGCCAGCCGATTGCACTGGTGGGCAGCAGTGG  >  NC_000913/3787531‑3788015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑