Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,787,485	G→A	E216E (GAG→GAA)	envC →	murein hydrolase activator EnvC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,787,485	0	G	A	100.0%	156.7 / NA	50	E216E (GAG→GAA)	envC	murein hydrolase activator EnvC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/28);  total (22/28)

CAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAGAGCGAGCAACAAACGCTGTTATATGAGCAGCGCGCCCAACAGGCGAAACTGACTCAGGCGCTGAACGAGCGTAAAAAGACGCTGGCAGGGCTGGAGTCTTCCATCCAGCAAGGTCAGCAACAG‑TTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGACGCGC‑AAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCC  >  NC_000913/3787248‑3787718                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                              cAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTtccatcc                                                                                                                                                                                                                                     <  1:94086/248‑1 (MQ=255)                 cAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTcagcagcagct                                                                                                                                                                                                                  >  1:241088/1‑249 (MQ=255)                       ggACAGCGTTTACAGGCTTCTTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGACCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGCATCTTCCATCCAACAAGGAcagcagcagccaagcg                                                                                                                                                                                                             >  2:353806/1‑243 (MQ=255)                           aGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTcagcagcagctaagtgaactg                                                                                                                                                                                                        <  1:92776/251‑13 (MQ=255)                              gTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTcagcagcagctaagtgaactg                                                                                                                                                                                                        <  1:155836/248‑13 (MQ=255)                                  aCAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTcagcagcagctaagtgaactgcgtgcc                                                                                                                                                                                                  <  1:103042/250‑19 (MQ=255)                                            ttCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATccc                                                                                                                                                                                       <  2:408784/250‑1 (MQ=255)                                               ggCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAAc                                                                                                                                                                                              <  2:18480/240‑1 (MQ=255)                                               ggCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCGGGGATCTTCCATCCAGCAAGGTcagcagcagccaaatgaactgcgtgcccac                                                                                                                                                                                               >  1:18480/1‑219 (MQ=255)                                                gCTATCTCAACCAGGCGAGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGCGTTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGGGATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCACCCTGCAAGGTCAGCAGCAG‑CCAAGTGAGCTGCGTGCCAACGAATCCCGTCTg                                                                                                                                                                                  <  2:357750/251‑1 (MQ=255)                                                     ctcAACCAGGCGCAACAAGAACCCATTGCACAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAAACTTCCATCCCGCAAGGGCAGCAGCAG‑CTAAGTGAACTGCGTGACAACGAAACCCGTCTGCGTa                                                                                                                                                                              >  2:418400/1‑250 (MQ=255)                                                                   aCAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCTGCAGCAG‑CTAAGTGAACGGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTg                                                                                                                                                               <  2:193804/251‑1 (MQ=255)                                                                    aaaGAATCCATTGCCCAGGTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTGATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAATACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTTAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGc                                                                                                                                                              <  1:279612/250‑1 (MQ=255)                                                                     aaGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCg                                                                                                                                                             <  2:241088/251‑1 (MQ=255)                                                                            cATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAACATTGCCCGTGCGG‑AAACcg                                                                                                                                                     >  1:174083/1‑251 (MQ=255)                                                                                          aGCAAACGCGTGAAGAAGTCGCCATGCAGCGAGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTACATCCCGCAAGGTCAGCAGCAG‑CTAAGGGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGGAAGCGCGt                                                                                                                                       >  1:154021/1‑251 (MQ=255)                                                                                           gCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCATCCAACCAGCCACGGCAGCAGCAG‑CTACGTGAACTGCGGGGCAACGGATCCCGACCGCGTCACAGCCATGCCCGTTCGGGAAGC‑GCGGCGGAAGcgcggg                                                                                                                                      >  2:313958/1‑249 (MQ=255)                                                                                            cAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGGATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACCGCATTTCCCGCGCGG‑AAGCCGCGGCGAAAGcgcgggg                                                                                                                                     >  2:252267/1‑248 (MQ=255)                                                                                            cAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGGGCCCACGAATCCCGTCTGCGGAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGCAAGcgcgggc                                                                                                                                     >  2:89589/1‑248 (MQ=255)                                                                                             aaaCGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCCTCCAGCAAGGGCAGCAGCAG‑CTAAGAGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCACGTGc                                                                                                                                     >  2:124466/1‑250 (MQ=255)                                                                                                  cgTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCCTCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACg                                                                                                                               >  2:169004/1‑251 (MQ=255)                                                                                                  cgTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑CAGCCGCGGCGAAAGCGCGTGCAGAACg                                                                                                                               >  1:45545/1‑251 (MQ=255)                                                                                                   gTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAACAGACGCTGGCGGGGCTGGTATCTTCCATTCAGCAAGGGCAGCACCAG‑CTAAATTAAATGCGTGCCAACGAATACCGTTTGCGTAACAGCATTGCCCGTGTGG‑AAGCCGCGCGGAAAGCGCGTgtggaacgg                                                                                                                              >  2:107404/1‑243 (MQ=255)                                                                                                     gaagaaGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGATGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATATTTCCTCCAGCAAGGGCAACCGCAGCTTAAG‑GAACTGCGGGCCAACGAATCCCGTCTGCGTAAAAGCATTGTCCCTGCGG‑AAACCGCGGCGAAAGCGCGGGCAGAACg                                                                                                                               >  2:393278/1‑248 (MQ=255)                                                                                                     gaagaaGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGAAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGGCAGCAGCAG‑CTAAGTGAACTGCGGGCCAACGAATCCCGTCTGCGTAACCGCATTGCCCGTGCGG‑AAACCGCGGCGGAAGCGCGTGCAAAACg                                                                                                                               >  1:409280/1‑248 (MQ=255)                                                                                                      aagaagTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGaa                                                                                                                           <  1:393278/251‑1 (MQ=255)                                                                                                        gaagTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGc                                                                                                                         <  1:332296/251‑1 (MQ=255)                                                                                                             cGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTg                                                                                                                    <  2:99533/251‑1 (MQ=255)                                                                                                                cATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAgg                                                                                                                 <  1:185236/251‑1 (MQ=255)                                                                                                                             gAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCGCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑tt                                                                                                     <  2:232203/249‑1 (MQ=255)                                                                                                                                   gAAGAGAAACAAAGCGAGCAACAAACGCTGTTAGATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CGAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCg                                                                                             <  1:293532/251‑1 (MQ=255)                                                                                                                                           acaaagcgagcAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCcgc                                                                                                                                                    <  1:245533/185‑1 (MQ=255)                                                                                                                                              aagcgagcAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCCAGGTCAGCAGCAG‑CTAAACGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGGGCAAAACGAGAAGCACGTGAGGACCAGGCGG‑GTCGCGACCGcccggaaaa                                                                                    >  1:82437/2‑242 (MQ=255)                                                                                                                                                gcgagcAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCCGGAATCTTCCCTCCAGCAAGGTCCGCAGCAG‑CTAAGTGAAATGCGTGCCAACGAATCCCGTCTGCGTAACAGCCTTGCCCGTGCGG‑GAGCCGCGGCGAAAACGCGTGCAGAACGGGAAGCACGGGAGGCCCAGGCGGGTTCGGGAcccccgaaaagagcg                                                                                 >  2:348655/1‑238 (MQ=255)                                                                                                                                                   agcAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTTCATCCACCAAAGTCAGCATCCA‑CCAAGTGAACTGCGGGCCCAAGAATCCCGTCCGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCTGCGAAAGCGCGCGCCGAACGGGCAGCACGCGAGGCCCAGGCTG‑TTtgcggccccccgacaaaagccacc                                                                             >  1:167138/1‑231 (MQ=255)                                                                                                                                                   agcAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGAcg                                                                             <  1:416484/251‑1 (MQ=255)                                                                                                                                                          aaCGCGGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGACGCGC‑AAAg                                                                     <  2:338698/251‑1 (MQ=255)                                                                                                                                                             gCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGACGCGC‑AAAGGca                                                                  <  1:348655/251‑1 (MQ=255)                                                                                                                                                                gTTATAGGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATGGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGACGCGC‑AAAGGcacc                                                                <  2:192064/250‑1 (MQ=255)                                                                                                                                                                    aatGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCCGCCG‑CTCAGTGAACTGCGTGCCAACGAATCCCGGCTGCGGAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTTCCGAACGAGAAGAACGTGAGGCCCCGGAGG‑GTCGCGAACGCCCGAAAGAAGGGGAGCCC‑AAAGGcaccccccc                                                           >  2:10733/2‑246 (MQ=255)                                                                                                                                                                          cagcGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGACGCGC‑AAAGGCACCACCTACAAacc                                                     <  1:344913/251‑1 (MQ=255)                                                                                                                                                                              gcgcCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGACGCGC‑AAAGGCACCACCTACAAaccgacc                                                 <  1:13138/251‑1 (MQ=255)                                                                                                                                                                                      cAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGACGCGC‑AAAGGCACCACCTACAAACCGACCGAAAGCGa                                         <  1:145293/251‑1 (MQ=255)                                                                                                                                                                                          cGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCTTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGACGCGC‑AAAGGCACCACCTACAAACCGACCGAAAGCGaaaaa                                     <  1:407864/251‑1 (MQ=255)                                                                                                                                                                                                tGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCCG‑CTAAGGGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCCGGCGG‑GTCGCGACCCCCCGAAAGAAACGACGCGC‑CAAGGCACCCCCTACAAACCCACCGAAAACGGAAAAACGCTg                               >  1:413677/1‑251 (MQ=255)                                                                                                                                                                                                  aCTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGGATCTTCAATCCAGCAAGGTCAGCCGCAG‑CTAAGTGAACTGCGTGGCAAAGAATCCCGTCTGCGAAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑GTCCGGAACGCCAGAAAAAAACGAGGCGCCAAAGGC‑CCACCTACAAACCGCACGAAAAGGAAAAATCGCTGAt                             >  1:138582/1‑251 (MQ=255)                                                                                                                                                                                                    ccAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGACGCGC‑AAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGt                           <  2:155294/250‑1 (MQ=255)                                                                                                                                                                                                               cgacgctctgccgatcGCTGGCGGGGCTGGAATCTTCCATCCATCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAAGCCCGGCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGACGCGC‑AAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCTCTGAGGTCCCgaactggt                <  2:295339/236‑8 (MQ=255)                                                                                                                                                                                                                             aCGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTTAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGACGCGC‑AAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGAACTGGTGGCCTGGGTGcgcc  <  1:141324/251‑1 (MQ=255)                                                                                                                                                                                                                                cTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAAATGCGTGCCAACGAATCCCGTCTGCGTAACCGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCCCGGGAGGCCCCGGCGG‑TTCGCGACCGTCCGAAAGAAGAGGCGCGC‑AAAGGCACCACCTCCCAACCGACCGAAAGCGAAAAATGGCTGGTGTCCCgccctggt                >  1:295339/1‑227 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                              CAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAGAGCGAGCAACAAACGCTGTTATATGAGCAGCGCGCCCAACAGGCGAAACTGACTCAGGCGCTGAACGAGCGTAAAAAGACGCTGGCAGGGCTGGAGTCTTCCATCCAGCAAGGTCAGCAACAG‑TTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGACGCGC‑AAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGTCCCGTACCGGTGGTCTGGGCGCGCC  >  NC_000913/3787248‑3787718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑