Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,787,455	C→T	N206N (AAC→AAT)	envC →	murein hydrolase activator EnvC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,787,455	0	C	T	100.0%	169.8 / NA	51	N206N (AAC→AAT)	envC	murein hydrolase activator EnvC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/29);  total (22/29)

GCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAGAGCGAGCAACAAACGCTGTTATATGAGCAGCGCGCCCAACAGGCGAAACTGACTCAGGCGCTGAACGAGCGTAAAAAGACGCTGGCAGGGCTGGAGTCTTCCATCCAGCAAGGTCAGCAACAG‑TTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGACGCGC‑AAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGT  >  NC_000913/3787220‑3787693                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                   gCGAACATACCTGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTGCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGTCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACg                                                                                                                                                                                                                                     <  1:185295/251‑1 (MQ=255)   gaacATACCGGTATTCAACTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCt                                                                                                                                                                                                                                   >  1:134608/4‑251 (MQ=255)    aacATACAGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAATCGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGTCTCTGAATGAGCGTAAAAAGACGCTg                                                                                                                                                                                                                                  <  2:333125/249‑1 (MQ=255)       aTACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGcg                                                                                                                                                                                                                               <  1:354771/251‑2 (MQ=255)                             cAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTtccatcc                                                                                                                                                                                                            <  1:94086/248‑1 (MQ=255)                                             cAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTcagcagcagct                                                                                                                                                                                         >  1:241088/1‑249 (MQ=255)                                                   ggACAGCGTTTACAGGCTTCTTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGACCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGCATCTTCCATCCAACAAGGAcagcagcagccaagcg                                                                                                                                                                                    >  2:353806/1‑243 (MQ=255)                                                       aGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTcagcagcagctaagtgaactg                                                                                                                                                                               <  1:92776/251‑13 (MQ=255)                                                          gTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTcagcagcagctaagtgaactg                                                                                                                                                                               <  1:155836/248‑13 (MQ=255)                                                              aCAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTcagcagcagctaagtgaactgcgtgcc                                                                                                                                                                         <  1:103042/250‑19 (MQ=255)                                                                        ttCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATccc                                                                                                                                                              <  2:408784/250‑1 (MQ=255)                                                                           ggCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAAc                                                                                                                                                                     <  2:18480/240‑1 (MQ=255)                                                                           ggCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCGGGGATCTTCCATCCAGCAAGGTcagcagcagccaaatgaactgcgtgcccac                                                                                                                                                                      >  1:18480/1‑219 (MQ=255)                                                                            gCTATCTCAACCAGGCGAGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGCGTTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGGGATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCACCCTGCAAGGTCAGCAGCAG‑CCAAGTGAGCTGCGTGCCAACGAATCCCGTCTg                                                                                                                                                         <  2:357750/251‑1 (MQ=255)                                                                                 ctcAACCAGGCGCAACAAGAACCCATTGCACAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAAACTTCCATCCCGCAAGGGCAGCAGCAG‑CTAAGTGAACTGCGTGACAACGAAACCCGTCTGCGTa                                                                                                                                                     >  2:418400/1‑250 (MQ=255)                                                                                               aCAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCTGCAGCAG‑CTAAGTGAACGGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTg                                                                                                                                      <  2:193804/251‑1 (MQ=255)                                                                                                aaaGAATCCATTGCCCAGGTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTGATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAATACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTTAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGc                                                                                                                                     <  1:279612/250‑1 (MQ=255)                                                                                                 aaGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCg                                                                                                                                    <  2:241088/251‑1 (MQ=255)                                                                                                        cATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAACATTGCCCGTGCGG‑AAACcg                                                                                                                            >  1:174083/1‑251 (MQ=255)                                                                                                                      aGCAAACGCGTGAAGAAGTCGCCATGCAGCGAGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTACATCCCGCAAGGTCAGCAGCAG‑CTAAGGGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGGAAGCGCGt                                                                                                              >  1:154021/1‑251 (MQ=255)                                                                                                                       gCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCATCCAACCAGCCACGGCAGCAGCAG‑CTACGTGAACTGCGGGGCAACGGATCCCGACCGCGTCACAGCCATGCCCGTTCGGGAAGC‑GCGGCGGAAGcgcggg                                                                                                             >  2:313958/1‑249 (MQ=255)                                                                                                                        cAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGGATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACCGCATTTCCCGCGCGG‑AAGCCGCGGCGAAAGcgcgggg                                                                                                            >  2:252267/1‑248 (MQ=255)                                                                                                                        cAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGGGCCCACGAATCCCGTCTGCGGAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGCAAGcgcgggc                                                                                                            >  2:89589/1‑248 (MQ=255)                                                                                                                         aaaCGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCCTCCAGCAAGGGCAGCAGCAG‑CTAAGAGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCACGTGc                                                                                                            >  2:124466/1‑250 (MQ=255)                                                                                                                              cgTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCCTCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACg                                                                                                      >  2:169004/1‑251 (MQ=255)                                                                                                                              cgTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑CAGCCGCGGCGAAAGCGCGTGCAGAACg                                                                                                      >  1:45545/1‑251 (MQ=255)                                                                                                                               gTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAACAGACGCTGGCGGGGCTGGTATCTTCCATTCAGCAAGGGCAGCACCAG‑CTAAATTAAATGCGTGCCAACGAATACCGTTTGCGTAACAGCATTGCCCGTGTGG‑AAGCCGCGCGGAAAGCGCGTgtggaacgg                                                                                                     >  2:107404/1‑243 (MQ=255)                                                                                                                                 gaagaaGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGATGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATATTTCCTCCAGCAAGGGCAACCGCAGCTTAAG‑GAACTGCGGGCCAACGAATCCCGTCTGCGTAAAAGCATTGTCCCTGCGG‑AAACCGCGGCGAAAGCGCGGGCAGAACg                                                                                                      >  2:393278/1‑248 (MQ=255)                                                                                                                                 gaagaaGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGAAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGGCAGCAGCAG‑CTAAGTGAACTGCGGGCCAACGAATCCCGTCTGCGTAACCGCATTGCCCGTGCGG‑AAACCGCGGCGGAAGCGCGTGCAAAACg                                                                                                      >  1:409280/1‑248 (MQ=255)                                                                                                                                  aagaagTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGaa                                                                                                  <  1:393278/251‑1 (MQ=255)                                                                                                                                    gaagTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGc                                                                                                <  1:332296/251‑1 (MQ=255)                                                                                                                                         cGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTg                                                                                           <  2:99533/251‑1 (MQ=255)                                                                                                                                            cATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAgg                                                                                        <  1:185236/251‑1 (MQ=255)                                                                                                                                                         gAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCGCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑tt                                                                            <  2:232203/249‑1 (MQ=255)                                                                                                                                                               gAAGAGAAACAAAGCGAGCAACAAACGCTGTTAGATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CGAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCg                                                                    <  1:293532/251‑1 (MQ=255)                                                                                                                                                                       acaaagcgagcAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCcgc                                                                                                                           <  1:245533/185‑1 (MQ=255)                                                                                                                                                                          aagcgagcAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCCAGGTCAGCAGCAG‑CTAAACGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGGGCAAAACGAGAAGCACGTGAGGACCAGGCGG‑GTCGCGACCGcccggaaaa                                                           >  1:82437/2‑242 (MQ=255)                                                                                                                                                                            gcgagcAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCCGGAATCTTCCCTCCAGCAAGGTCCGCAGCAG‑CTAAGTGAAATGCGTGCCAACGAATCCCGTCTGCGTAACAGCCTTGCCCGTGCGG‑GAGCCGCGGCGAAAACGCGTGCAGAACGGGAAGCACGGGAGGCCCAGGCGGGTTCGGGAcccccgaaaagagcg                                                        >  2:348655/1‑238 (MQ=255)                                                                                                                                                                               agcAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTTCATCCACCAAAGTCAGCATCCA‑CCAAGTGAACTGCGGGCCCAAGAATCCCGTCCGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCTGCGAAAGCGCGCGCCGAACGGGCAGCACGCGAGGCCCAGGCTG‑TTtgcggccccccgacaaaagccacc                                                    >  1:167138/1‑231 (MQ=255)                                                                                                                                                                               agcAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGAcg                                                    <  1:416484/251‑1 (MQ=255)                                                                                                                                                                                      aaCGCGGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGACGCGC‑AAAg                                            <  2:338698/251‑1 (MQ=255)                                                                                                                                                                                         gCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGACGCGC‑AAAGGca                                         <  1:348655/251‑1 (MQ=255)                                                                                                                                                                                            gTTATAGGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATGGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGACGCGC‑AAAGGcacc                                       <  2:192064/250‑1 (MQ=255)                                                                                                                                                                                                aatGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCCGCCG‑CTCAGTGAACTGCGTGCCAACGAATCCCGGCTGCGGAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTTCCGAACGAGAAGAACGTGAGGCCCCGGAGG‑GTCGCGAACGCCCGAAAGAAGGGGAGCCC‑AAAGGcaccccccc                                  >  2:10733/2‑246 (MQ=255)                                                                                                                                                                                                      cagcGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGACGCGC‑AAAGGCACCACCTACAAacc                            <  1:344913/251‑1 (MQ=255)                                                                                                                                                                                                          gcgcCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGACGCGC‑AAAGGCACCACCTACAAaccgacc                        <  1:13138/251‑1 (MQ=255)                                                                                                                                                                                                                  cAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGACGCGC‑AAAGGCACCACCTACAAACCGACCGAAAGCGa                <  1:145293/251‑1 (MQ=255)                                                                                                                                                                                                                      cGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCTTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGACGCGC‑AAAGGCACCACCTACAAACCGACCGAAAGCGaaaaa            <  1:407864/251‑1 (MQ=255)                                                                                                                                                                                                                            tGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCCG‑CTAAGGGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCCGGCGG‑GTCGCGACCCCCCGAAAGAAACGACGCGC‑CAAGGCACCCCCTACAAACCCACCGAAAACGGAAAAACGCTg      >  1:413677/1‑251 (MQ=255)                                                                                                                                                                                                                              aCTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGGATCTTCAATCCAGCAAGGTCAGCCGCAG‑CTAAGTGAACTGCGTGGCAAAGAATCCCGTCTGCGAAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑GTCCGGAACGCCAGAAAAAAACGAGGCGCCAAAGGC‑CCACCTACAAACCGCACGAAAAGGAAAAATCGCTGAt    >  1:138582/1‑251 (MQ=255)                                                                                                                                                                                                                                ccAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGACGCGC‑AAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGt  <  2:155294/250‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                   GCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAGAGCGAGCAACAAACGCTGTTATATGAGCAGCGCGCCCAACAGGCGAAACTGACTCAGGCGCTGAACGAGCGTAAAAAGACGCTGGCAGGGCTGGAGTCTTCCATCCAGCAAGGTCAGCAACAG‑TTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGACGCGC‑AAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGT  >  NC_000913/3787220‑3787693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑