| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,787,449 | G→T | A204A (GCG→GCT) | envC → | murein hydrolase activator EnvC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,787,449 | 0 | G | T | 98.1% | 169.1 / NA | 53 | A204A (GCG→GCT) | envC | murein hydrolase activator EnvC |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (23/29); total (23/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
GCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAGAGCGAGCAACAAACGCTGTTATATGAGCAGCGCGCCCAACAGGCGAAACTGACTCAGGCGCTGAACGAGCGTAAAAAGACGCTGGCAGGGCTGGAGTCTTCCATCCAGCAAGGTCAGCAACAG‑TTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGACGCGC‑AAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGT > NC_000913/3787220‑3787693 | gCGAACATACCTGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTGCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGTCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACg < 1:185295/251‑1 (MQ=255) gaacATACCGGTATTCAACTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCt > 1:134608/4‑251 (MQ=255) aacATACAGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAATCGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGTCTCTGAATGAGCGTAAAAAGACGCTg < 2:333125/249‑1 (MQ=255) aTACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGcg < 1:354771/251‑2 (MQ=255) cAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTg > 1:96816/1‑205 (MQ=255) cAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTg < 2:96816/205‑1 (MQ=255) cAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTtccatcc < 1:94086/248‑1 (MQ=255) cAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTcagcagcagct > 1:241088/1‑249 (MQ=255) ggACAGCGTTTACAGGCTTCTTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGACCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGCATCTTCCATCCAACAAGGAcagcagcagccaagcg > 2:353806/1‑243 (MQ=255) aGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTcagcagcagctaagtgaactg < 1:92776/251‑13 (MQ=255) gTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTcagcagcagctaagtgaactg < 1:155836/248‑13 (MQ=255) aCAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTcagcagcagctaagtgaactgcgtgcc < 1:103042/250‑19 (MQ=255) ttCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATccc < 2:408784/250‑1 (MQ=255) ggCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAAc < 2:18480/240‑1 (MQ=255) ggCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCGGGGATCTTCCATCCAGCAAGGTcagcagcagccaaatgaactgcgtgcccac > 1:18480/1‑219 (MQ=255) gCTATCTCAACCAGGCGAGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGCGTTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGGGATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCACCCTGCAAGGTCAGCAGCAG‑CCAAGTGAGCTGCGTGCCAACGAATCCCGTCTg < 2:357750/251‑1 (MQ=255) ctcAACCAGGCGCAACAAGAACCCATTGCACAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAAACTTCCATCCCGCAAGGGCAGCAGCAG‑CTAAGTGAACTGCGTGACAACGAAACCCGTCTGCGTa > 2:418400/1‑250 (MQ=255) aCAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCTGCAGCAG‑CTAAGTGAACGGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTg < 2:193804/251‑1 (MQ=255) aaaGAATCCATTGCCCAGGTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTGATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAATACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTTAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGc < 1:279612/250‑1 (MQ=255) aaGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCg < 2:241088/251‑1 (MQ=255) cATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAACATTGCCCGTGCGG‑AAACcg > 1:174083/1‑251 (MQ=255) aGCAAACGCGTGAAGAAGTCGCCATGCAGCGAGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTACATCCCGCAAGGTCAGCAGCAG‑CTAAGGGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGGAAGCGCGt > 1:154021/1‑251 (MQ=255) gCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCATCCAACCAGCCACGGCAGCAGCAG‑CTACGTGAACTGCGGGGCAACGGATCCCGACCGCGTCACAGCCATGCCCGTTCGGGAAGC‑GCGGCGGAAGcgcggg > 2:313958/1‑249 (MQ=255) cAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGGATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACCGCATTTCCCGCGCGG‑AAGCCGCGGCGAAAGcgcgggg > 2:252267/1‑248 (MQ=255) cAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGGGCCCACGAATCCCGTCTGCGGAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGCAAGcgcgggc > 2:89589/1‑248 (MQ=255) aaaCGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCCTCCAGCAAGGGCAGCAGCAG‑CTAAGAGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCACGTGc > 2:124466/1‑250 (MQ=255) cgTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCCTCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACg > 2:169004/1‑251 (MQ=255) cgTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑CAGCCGCGGCGAAAGCGCGTGCAGAACg > 1:45545/1‑251 (MQ=255) gTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAACAGACGCTGGCGGGGCTGGTATCTTCCATTCAGCAAGGGCAGCACCAG‑CTAAATTAAATGCGTGCCAACGAATACCGTTTGCGTAACAGCATTGCCCGTGTGG‑AAGCCGCGCGGAAAGCGCGTgtggaacgg > 2:107404/1‑243 (MQ=255) gaagaaGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGATGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATATTTCCTCCAGCAAGGGCAACCGCAGCTTAAG‑GAACTGCGGGCCAACGAATCCCGTCTGCGTAAAAGCATTGTCCCTGCGG‑AAACCGCGGCGAAAGCGCGGGCAGAACg > 2:393278/1‑248 (MQ=255) gaagaaGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGAAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGGCAGCAGCAG‑CTAAGTGAACTGCGGGCCAACGAATCCCGTCTGCGTAACCGCATTGCCCGTGCGG‑AAACCGCGGCGGAAGCGCGTGCAAAACg > 1:409280/1‑248 (MQ=255) aagaagTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGaa < 1:393278/251‑1 (MQ=255) gaagTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGc < 1:332296/251‑1 (MQ=255) cGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTg < 2:99533/251‑1 (MQ=255) cATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAgg < 1:185236/251‑1 (MQ=255) gAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCGCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑tt < 2:232203/249‑1 (MQ=255) gAAGAGAAACAAAGCGAGCAACAAACGCTGTTAGATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CGAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCg < 1:293532/251‑1 (MQ=255) acaaagcgagcAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCcgc < 1:245533/185‑1 (MQ=255) aagcgagcAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCCAGGTCAGCAGCAG‑CTAAACGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGGGCAAAACGAGAAGCACGTGAGGACCAGGCGG‑GTCGCGACCGcccggaaaa > 1:82437/2‑242 (MQ=255) gcgagcAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCCGGAATCTTCCCTCCAGCAAGGTCCGCAGCAG‑CTAAGTGAAATGCGTGCCAACGAATCCCGTCTGCGTAACAGCCTTGCCCGTGCGG‑GAGCCGCGGCGAAAACGCGTGCAGAACGGGAAGCACGGGAGGCCCAGGCGGGTTCGGGAcccccgaaaagagcg > 2:348655/1‑238 (MQ=255) agcAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTTCATCCACCAAAGTCAGCATCCA‑CCAAGTGAACTGCGGGCCCAAGAATCCCGTCCGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCTGCGAAAGCGCGCGCCGAACGGGCAGCACGCGAGGCCCAGGCTG‑TTtgcggccccccgacaaaagccacc > 1:167138/1‑231 (MQ=255) agcAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGAcg < 1:416484/251‑1 (MQ=255) aaCGCGGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGACGCGC‑AAAg < 2:338698/251‑1 (MQ=255) gCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGACGCGC‑AAAGGca < 1:348655/251‑1 (MQ=255) gTTATAGGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATGGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGACGCGC‑AAAGGcacc < 2:192064/250‑1 (MQ=255) aatGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCCGCCG‑CTCAGTGAACTGCGTGCCAACGAATCCCGGCTGCGGAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTTCCGAACGAGAAGAACGTGAGGCCCCGGAGG‑GTCGCGAACGCCCGAAAGAAGGGGAGCCC‑AAAGGcaccccccc > 2:10733/2‑246 (MQ=255) cagcGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGACGCGC‑AAAGGCACCACCTACAAacc < 1:344913/251‑1 (MQ=255) gcgcCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGACGCGC‑AAAGGCACCACCTACAAaccgacc < 1:13138/251‑1 (MQ=255) cAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGACGCGC‑AAAGGCACCACCTACAAACCGACCGAAAGCGa < 1:145293/251‑1 (MQ=255) cGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCTTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGACGCGC‑AAAGGCACCACCTACAAACCGACCGAAAGCGaaaaa < 1:407864/251‑1 (MQ=255) tGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCCG‑CTAAGGGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCCGGCGG‑GTCGCGACCCCCCGAAAGAAACGACGCGC‑CAAGGCACCCCCTACAAACCCACCGAAAACGGAAAAACGCTg > 1:413677/1‑251 (MQ=255) aCTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGGATCTTCAATCCAGCAAGGTCAGCCGCAG‑CTAAGTGAACTGCGTGGCAAAGAATCCCGTCTGCGAAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑GTCCGGAACGCCAGAAAAAAACGAGGCGCCAAAGGC‑CCACCTACAAACCGCACGAAAAGGAAAAATCGCTGAt > 1:138582/1‑251 (MQ=255) ccAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGACGCGC‑AAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGt < 2:155294/250‑1 (MQ=255) | GCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAGAGCGAGCAACAAACGCTGTTATATGAGCAGCGCGCCCAACAGGCGAAACTGACTCAGGCGCTGAACGAGCGTAAAAAGACGCTGGCAGGGCTGGAGTCTTCCATCCAGCAAGGTCAGCAACAG‑TTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGACGCGC‑AAAGGCACCACCTACAAACCGACCGAAAGCGAAAAATCGCTGATGT > NC_000913/3787220‑3787693 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |