Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,787,416	G→A	E193E (GAG→GAA)	envC →	murein hydrolase activator EnvC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,787,416	0	G	A	100.0%	164.5 / NA	49	E193E (GAG→GAA)	envC	murein hydrolase activator EnvC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/25);  total (24/25)

CAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAGAGCGAGCAACAAACGCTGTTATATGAGCAGCGCGCCCAACAGGCGAAACTGACTCAGGCGCTGAACGAGCGTAAAAAGACGCTGGCAGGGCTGGAGTCTTCCATCCAGCAAGGTCAGCAACAG‑TTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTA  >  NC_000913/3787174‑3787661                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                         cAGGAGCGCAGCCACGCCGCACAACTGGATGCCGCATTCCTTCAGGGCGAACATACCGGCATTAAGCTGATTATCAGCGGTGAAGAACGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTGTCTCAACCCGACGCGACAAGAAACCATTGCACAGTTGAAGCAAACGAGTGAAGAAGTCGCCATGCAGCGTGCTGACCTGGCAGAGAAACAAACCGAGCAACACAGGCTGTTAAATGAACAgcgcgc                                                                                                                                                                                                                                                  >  2:254851/1‑251 (MQ=255)  aGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGcc                                                                                                                                                                                                                                                 >  1:338698/1‑251 (MQ=255)                         cTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGgc                                                                                                                                                                                                                          >  1:313201/1‑251 (MQ=255)                                               gCGAACATACCTGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTGCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGTCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACg                                                                                                                                                                                                    <  1:185295/251‑1 (MQ=255)                                                 gaacATACCGGTATTCAACTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCt                                                                                                                                                                                                  >  1:134608/4‑251 (MQ=255)                                                  aacATACAGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAATCGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGTCTCTGAATGAGCGTAAAAAGACGCTg                                                                                                                                                                                                 <  2:333125/249‑1 (MQ=255)                                                     aTACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGcg                                                                                                                                                                                              <  1:354771/251‑2 (MQ=255)                                                                           cAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTg                                                                                                                                                                                                                      <  2:96816/205‑1 (MQ=255)                                                                           cAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTg                                                                                                                                                                                                                      >  1:96816/1‑205 (MQ=255)                                                                           cAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTtccatcc                                                                                                                                                                           <  1:94086/248‑1 (MQ=255)                                                                                           cAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTcagcagcagct                                                                                                                                                        >  1:241088/1‑249 (MQ=255)                                                                                                 ggACAGCGTTTACAGGCTTCTTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGACCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGCATCTTCCATCCAACAAGGAcagcagcagccaagcg                                                                                                                                                   >  2:353806/1‑243 (MQ=255)                                                                                                     aGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTcagcagcagctaagtgaactg                                                                                                                                              <  1:92776/251‑13 (MQ=255)                                                                                                        gTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTcagcagcagctaagtgaactg                                                                                                                                              <  1:155836/248‑13 (MQ=255)                                                                                                            aCAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTcagcagcagctaagtgaactgcgtgcc                                                                                                                                        <  1:103042/250‑19 (MQ=255)                                                                                                                      ttCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATccc                                                                                                                             <  2:408784/250‑1 (MQ=255)                                                                                                                         ggCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAAc                                                                                                                                    <  2:18480/240‑1 (MQ=255)                                                                                                                         ggCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCGGGGATCTTCCATCCAGCAAGGTcagcagcagccaaatgaactgcgtgcccac                                                                                                                                     >  1:18480/1‑219 (MQ=255)                                                                                                                          gCTATCTCAACCAGGCGAGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGCGTTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGGGATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCACCCTGCAAGGTCAGCAGCAG‑CCAAGTGAGCTGCGTGCCAACGAATCCCGTCTg                                                                                                                        <  2:357750/251‑1 (MQ=255)                                                                                                                               ctcAACCAGGCGCAACAAGAACCCATTGCACAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAAACTTCCATCCCGCAAGGGCAGCAGCAG‑CTAAGTGAACTGCGTGACAACGAAACCCGTCTGCGTa                                                                                                                    >  2:418400/1‑250 (MQ=255)                                                                                                                                             aCAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCTGCAGCAG‑CTAAGTGAACGGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTg                                                                                                     <  2:193804/251‑1 (MQ=255)                                                                                                                                              aaaGAATCCATTGCCCAGGTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTGATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAATACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTTAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGc                                                                                                    <  1:279612/250‑1 (MQ=255)                                                                                                                                               aaGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCg                                                                                                   <  2:241088/251‑1 (MQ=255)                                                                                                                                                      cATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAACATTGCCCGTGCGG‑AAACcg                                                                                           >  1:174083/1‑251 (MQ=255)                                                                                                                                                                    aGCAAACGCGTGAAGAAGTCGCCATGCAGCGAGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTACATCCCGCAAGGTCAGCAGCAG‑CTAAGGGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGGAAGCGCGt                                                                             >  1:154021/1‑251 (MQ=255)                                                                                                                                                                     gCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCATCCAACCAGCCACGGCAGCAGCAG‑CTACGTGAACTGCGGGGCAACGGATCCCGACCGCGTCACAGCCATGCCCGTTCGGGAAGC‑GCGGCGGAAGcgcggg                                                                            >  2:313958/1‑249 (MQ=255)                                                                                                                                                                      cAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGGATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACCGCATTTCCCGCGCGG‑AAGCCGCGGCGAAAGcgcgggg                                                                           >  2:252267/1‑248 (MQ=255)                                                                                                                                                                      cAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGGGCCCACGAATCCCGTCTGCGGAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGCAAGcgcgggc                                                                           >  2:89589/1‑248 (MQ=255)                                                                                                                                                                       aaaCGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCCTCCAGCAAGGGCAGCAGCAG‑CTAAGAGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCACGTGc                                                                           >  2:124466/1‑250 (MQ=255)                                                                                                                                                                            cgTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCCTCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACg                                                                     >  2:169004/1‑251 (MQ=255)                                                                                                                                                                            cgTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑CAGCCGCGGCGAAAGCGCGTGCAGAACg                                                                     >  1:45545/1‑251 (MQ=255)                                                                                                                                                                             gTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAACAGACGCTGGCGGGGCTGGTATCTTCCATTCAGCAAGGGCAGCACCAG‑CTAAATTAAATGCGTGCCAACGAATACCGTTTGCGTAACAGCATTGCCCGTGTGG‑AAGCCGCGCGGAAAGCGCGTgtggaacgg                                                                    >  2:107404/1‑243 (MQ=255)                                                                                                                                                                               gaagaaGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGATGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATATTTCCTCCAGCAAGGGCAACCGCAGCTTAAG‑GAACTGCGGGCCAACGAATCCCGTCTGCGTAAAAGCATTGTCCCTGCGG‑AAACCGCGGCGAAAGCGCGGGCAGAACg                                                                     >  2:393278/1‑248 (MQ=255)                                                                                                                                                                               gaagaaGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGAAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGGCAGCAGCAG‑CTAAGTGAACTGCGGGCCAACGAATCCCGTCTGCGTAACCGCATTGCCCGTGCGG‑AAACCGCGGCGGAAGCGCGTGCAAAACg                                                                     >  1:409280/1‑248 (MQ=255)                                                                                                                                                                                aagaagTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGaa                                                                 <  1:393278/251‑1 (MQ=255)                                                                                                                                                                                  gaagTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGc                                                               <  1:332296/251‑1 (MQ=255)                                                                                                                                                                                       cGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTg                                                          <  2:99533/251‑1 (MQ=255)                                                                                                                                                                                          cATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAgg                                                       <  1:185236/251‑1 (MQ=255)                                                                                                                                                                                                       gAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCGCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑tt                                           <  2:232203/249‑1 (MQ=255)                                                                                                                                                                                                             gAAGAGAAACAAAGCGAGCAACAAACGCTGTTAGATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CGAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCg                                   <  1:293532/251‑1 (MQ=255)                                                                                                                                                                                                                     acaaagcgagcAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCcgc                                                                                          <  1:245533/185‑1 (MQ=255)                                                                                                                                                                                                                        aagcgagcAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCCAGGTCAGCAGCAG‑CTAAACGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGGGCAAAACGAGAAGCACGTGAGGACCAGGCGG‑GTCGCGACCGcccggaaaa                          >  1:82437/2‑242 (MQ=255)                                                                                                                                                                                                                          gcgagcAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCCGGAATCTTCCCTCCAGCAAGGTCCGCAGCAG‑CTAAGTGAAATGCGTGCCAACGAATCCCGTCTGCGTAACAGCCTTGCCCGTGCGG‑GAGCCGCGGCGAAAACGCGTGCAGAACGGGAAGCACGGGAGGCCCAGGCGGGTTCGGGAcccccgaaaagagcg                       >  2:348655/1‑238 (MQ=255)                                                                                                                                                                                                                             agcAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTTCATCCACCAAAGTCAGCATCCA‑CCAAGTGAACTGCGGGCCCAAGAATCCCGTCCGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCTGCGAAAGCGCGCGCCGAACGGGCAGCACGCGAGGCCCAGGCTG‑TTtgcggccccccgacaaaagccacc                   >  1:167138/1‑231 (MQ=255)                                                                                                                                                                                                                             agcAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGAcg                   <  1:416484/251‑1 (MQ=255)                                                                                                                                                                                                                                    aaCGCGGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGACGCGCAAAg            <  2:338698/251‑1 (MQ=255)                                                                                                                                                                                                                                       gCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGca         <  1:348655/251‑1 (MQ=255)                                                                                                                                                                                                                                          gTTATAGGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATGGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGcacc       <  2:192064/250‑1 (MQ=255)                                                                                                                                                                                                                                              aatGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCCGCCG‑CTCAGTGAACTGCGTGCCAACGAATCCCGGCTGCGGAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTTCCGAACGAGAAGAACGTGAGGCCCCGGAGG‑GTCGCGAACGCCCGAAAGAAGGGGAGCCCAAAGGcaccccccc  >  2:10733/2‑246 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                         CAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAGAGCGAGCAACAAACGCTGTTATATGAGCAGCGCGCCCAACAGGCGAAACTGACTCAGGCGCTGAACGAGCGTAAAAAGACGCTGGCAGGGCTGGAGTCTTCCATCCAGCAAGGTCAGCAACAG‑TTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGG‑TTCGCGACCGCCAGAAAGAAGCGACGCGCAAAGGCACCACCTA  >  NC_000913/3787174‑3787661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑