Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,787,389	G→A	Q184Q (CAG→CAA)	envC →	murein hydrolase activator EnvC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,787,389	0	G	A	100.0%	143.0 / NA	43	Q184Q (CAG→CAA)	envC	murein hydrolase activator EnvC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/21);  total (22/21)

GCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAGAGCGAGCAACAAACGCTGTTATATGAGCAGCGCGCCCAACAGGCGAAACTGACTCAGGCGCTGAACGAGCGTAAAAAGACGCTGGCAGGGCTGGAGTCTTCCATCCAGCAAGGTCAGCAACAG‑TTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCG  >  NC_000913/3787147‑3787628                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  gCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGGTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCACTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGca                                                                                                                                                                                                                                           <  2:273487/251‑1 (MQ=255)    aaaCTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGAGCTGAACTGGAAGAGAAACAAAGCGAGcaac                                                                                                                                                                                                                                         >  1:318573/1‑250 (MQ=255)        tGGAGCAGCAAAAAACCGCCCAGGAGCGCAGCATCGCCGCACAACTGGATGCAGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAGGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACg                                                                                                                                                                                                                                    >  1:226925/1‑251 (MQ=255)                            cAGGAGCGCAGCCACGCCGCACAACTGGATGCCGCATTCCTTCAGGGCGAACATACCGGCATTAAGCTGATTATCAGCGGTGAAGAACGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTGTCTCAACCCGACGCGACAAGAAACCATTGCACAGTTGAAGCAAACGAGTGAAGAAGTCGCCATGCAGCGTGCTGACCTGGCAGAGAAACAAACCGAGCAACACAGGCTGTTAAATGAACAgcgcgc                                                                                                                                                                                                                >  2:254851/1‑251 (MQ=255)                             aGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGcc                                                                                                                                                                                                               >  1:338698/1‑251 (MQ=255)                                                    cTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGgc                                                                                                                                                                                        >  1:313201/1‑251 (MQ=255)                                                                          gCGAACATACCTGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTGCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGTCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACg                                                                                                                                                                  <  1:185295/251‑1 (MQ=255)                                                                            gaacATACCGGTATTCAACTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCt                                                                                                                                                                >  1:134608/4‑251 (MQ=255)                                                                             aacATACAGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAATCGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGTCTCTGAATGAGCGTAAAAAGACGCTg                                                                                                                                                               <  2:333125/249‑1 (MQ=255)                                                                                aTACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGcg                                                                                                                                                            <  1:354771/251‑2 (MQ=255)                                                                                                      cAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTg                                                                                                                                                                                    <  2:96816/205‑1 (MQ=255)                                                                                                      cAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTg                                                                                                                                                                                    >  1:96816/1‑205 (MQ=255)                                                                                                      cAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTtccatcc                                                                                                                                         <  1:94086/248‑1 (MQ=255)                                                                                                                      cAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTcagcagcagct                                                                                                                      >  1:241088/1‑249 (MQ=255)                                                                                                                            ggACAGCGTTTACAGGCTTCTTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGACCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGCATCTTCCATCCAACAAGGAcagcagcagccaagcg                                                                                                                 >  2:353806/1‑243 (MQ=255)                                                                                                                                aGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTcagcagcagctaagtgaactg                                                                                                            <  1:92776/251‑13 (MQ=255)                                                                                                                                   gTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTcagcagcagctaagtgaactg                                                                                                            <  1:155836/248‑13 (MQ=255)                                                                                                                                       aCAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTcagcagcagctaagtgaactgcgtgcc                                                                                                      <  1:103042/250‑19 (MQ=255)                                                                                                                                                 ttCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATccc                                                                                           <  2:408784/250‑1 (MQ=255)                                                                                                                                                    ggCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAAc                                                                                                  <  2:18480/240‑1 (MQ=255)                                                                                                                                                    ggCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCGGGGATCTTCCATCCAGCAAGGTcagcagcagccaaatgaactgcgtgcccac                                                                                                   >  1:18480/1‑219 (MQ=255)                                                                                                                                                     gCTATCTCAACCAGGCGAGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGCGTTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGGGATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCACCCTGCAAGGTCAGCAGCAG‑CCAAGTGAGCTGCGTGCCAACGAATCCCGTCTg                                                                                      <  2:357750/251‑1 (MQ=255)                                                                                                                                                          ctcAACCAGGCGCAACAAGAACCCATTGCACAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAAACTTCCATCCCGCAAGGGCAGCAGCAG‑CTAAGTGAACTGCGTGACAACGAAACCCGTCTGCGTa                                                                                  >  2:418400/1‑250 (MQ=255)                                                                                                                                                                        aCAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCTGCAGCAG‑CTAAGTGAACGGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTg                                                                   <  2:193804/251‑1 (MQ=255)                                                                                                                                                                         aaaGAATCCATTGCCCAGGTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTGATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAATACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTTAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGc                                                                  <  1:279612/250‑1 (MQ=255)                                                                                                                                                                          aaGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCg                                                                 <  2:241088/251‑1 (MQ=255)                                                                                                                                                                                 cATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAACATTGCCCGTGCGG‑AAACcg                                                         >  1:174083/1‑251 (MQ=255)                                                                                                                                                                                               aGCAAACGCGTGAAGAAGTCGCCATGCAGCGAGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTACATCCCGCAAGGTCAGCAGCAG‑CTAAGGGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGGAAGCGCGt                                           >  1:154021/1‑251 (MQ=255)                                                                                                                                                                                                gCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCATCCAACCAGCCACGGCAGCAGCAG‑CTACGTGAACTGCGGGGCAACGGATCCCGACCGCGTCACAGCCATGCCCGTTCGGGAAGC‑GCGGCGGAAGcgcggg                                          >  2:313958/1‑249 (MQ=255)                                                                                                                                                                                                 cAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGGATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACCGCATTTCCCGCGCGG‑AAGCCGCGGCGAAAGcgcgggg                                         >  2:252267/1‑248 (MQ=255)                                                                                                                                                                                                 cAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGGGCCCACGAATCCCGTCTGCGGAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGCAAGcgcgggc                                         >  2:89589/1‑248 (MQ=255)                                                                                                                                                                                                  aaaCGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCCTCCAGCAAGGGCAGCAGCAG‑CTAAGAGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCACGTGc                                         >  2:124466/1‑250 (MQ=255)                                                                                                                                                                                                       cgTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCCTCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACg                                   >  2:169004/1‑251 (MQ=255)                                                                                                                                                                                                       cgTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑CAGCCGCGGCGAAAGCGCGTGCAGAACg                                   >  1:45545/1‑251 (MQ=255)                                                                                                                                                                                                        gTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAACAGACGCTGGCGGGGCTGGTATCTTCCATTCAGCAAGGGCAGCACCAG‑CTAAATTAAATGCGTGCCAACGAATACCGTTTGCGTAACAGCATTGCCCGTGTGG‑AAGCCGCGCGGAAAGCGCGTgtggaacgg                                  >  2:107404/1‑243 (MQ=255)                                                                                                                                                                                                          gaagaaGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGATGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATATTTCCTCCAGCAAGGGCAACCGCAGCTTAAG‑GAACTGCGGGCCAACGAATCCCGTCTGCGTAAAAGCATTGTCCCTGCGG‑AAACCGCGGCGAAAGCGCGGGCAGAACg                                   >  2:393278/1‑248 (MQ=255)                                                                                                                                                                                                          gaagaaGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGAAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGGCAGCAGCAG‑CTAAGTGAACTGCGGGCCAACGAATCCCGTCTGCGTAACCGCATTGCCCGTGCGG‑AAACCGCGGCGGAAGCGCGTGCAAAACg                                   >  1:409280/1‑248 (MQ=255)                                                                                                                                                                                                           aagaagTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGaa                               <  1:393278/251‑1 (MQ=255)                                                                                                                                                                                                             gaagTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGc                             <  1:332296/251‑1 (MQ=255)                                                                                                                                                                                                                  cGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTg                        <  2:99533/251‑1 (MQ=255)                                                                                                                                                                                                                     cATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAgg                     <  1:185236/251‑1 (MQ=255)                                                                                                                                                                                                                                  gAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCGCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CTAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGtt          <  2:232203/249‑1 (MQ=255)                                                                                                                                                                                                                                        gAAGAGAAACAAAGCGAGCAACAAACGCTGTTAGATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACGCTGGCGGGGCTGGAATCTTCCATCCAGCAAGGTCAGCAGCAG‑CGAAGTGAACTGCGTGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCg  <  1:293532/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  GCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAGAGCGAGCAACAAACGCTGTTATATGAGCAGCGCGCCCAACAGGCGAAACTGACTCAGGCGCTGAACGAGCGTAAAAAGACGCTGGCAGGGCTGGAGTCTTCCATCCAGCAAGGTCAGCAACAG‑TTGAGCGAGCTGCGCGCCAACGAATCCCGTCTGCGTAACAGCATTGCCCGTGCGG‑AAGCCGCGGCGAAAGCGCGTGCAGAACGAGAAGCACGTGAGGCCCAGGCGGTTCGCGACCG  >  NC_000913/3787147‑3787628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑