Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,787,224	G→A	E129E (GAG→GAA)	envC →	murein hydrolase activator EnvC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,787,224	0	G	A	100.0%	186.4 / NA	58	E129E (GAG→GAA)	envC	murein hydrolase activator EnvC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/25);  total (33/25)

CGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAGAGCGAGCAACAAACGCTGTTATATGAGCAGCGCGCCCAACAGGCGAAACTGACTCAGGCGCTGAACGAGCGTAAAAAGACG  >  NC_000913/3786978‑3787470                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       cgccgcGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACATCTGGATGCCGCATTCCGTCAGGGCGAACATa                                                                                                                                                                                                                                                    <  1:370714/251‑1 (MQ=255)      cgaaaGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCgg                                                                                                                                                                                                                                                >  1:403361/3‑250 (MQ=255)       gaaaGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCTCAGCCTCGCTGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTa                                                                                                                                                                                                                                              <  2:395725/250‑1 (MQ=255)           gagCGCGCGGTACGCCAAAAGCAACACCAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGAGGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCa                                                                                                                                                                                                                                          <  2:403361/251‑1 (MQ=255)                   agTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATtc                                                                                                                                                                                                                                  >  2:103042/2‑251 (MQ=255)                    gTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATtct                                                                                                                                                                                                                                 >  2:260662/1‑251 (MQ=255)                      aCGCCAAAAGCAAAAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGGTtctc                                                                                                                                                                                                                                >  2:79836/1‑250 (MQ=255)                        gCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAACACGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCACTAAACAGCTTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGc                                                                                                                                                                                                                             >  2:293532/1‑251 (MQ=255)                          cAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCgg                                                                                                                                                                                                                           >  1:309112/1‑251 (MQ=255)                           aaaaGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGt                                                                                                                                                                                                                          <  1:272163/251‑1 (MQ=255)                                  acaacaacGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGAGCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGaaa                                                                                                                                                                                                                   <  2:152920/251‑1 (MQ=255)                                         cgatcgaGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGAGGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCg                                                                                                                                                                                                            <  1:228260/245‑1 (MQ=255)                                          gcgcCAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGACAGCCAGCGt                                                                                                                                                                                                           >  1:192064/1‑251 (MQ=255)                                              aaGCCTGCTAGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGCAGCCACCCGTAAGCTGCGCGAAACGCACAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGAATTCAGCTGATTCTCAGCGGGGAAGACAGCCAGCGTgaa                                                                                                                                                                                                        >  2:194926/1‑248 (MQ=255)                                               aGCCTGCTCGCAAAATTGAAACAGCAGGACGCAGCGATCTATGAAGCCACCCGTAAGCTGCGCGACACGCAAAACACGCTCAATCAACTAAATAAACCGATTGATGAGCTGAACGAGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCCGGAGAACCGCCTCGCCGCACCACTGGATGCCGCATTCAGTCAGGGCGAAAATACCGGTATTCAACTGATTCTCAGCGGTGAAGAAAGCCAGCg                                                                                                                                                                                                            >  2:228260/1‑245 (MQ=255)                                                           caagtGAAAAAGAAGGCAGAAGCGCTTACTGAAGACACCCGTAAGCTGCGCGAAACTCAAAACGCGCTCAATCAACTCAATAAGCAGATTGATGAGATGAACGCGTCGCTTGCCTAACTGGAGCAGCCAAAAGCCGCCCAGGAGCGCAGCCGCGCCGCTCAACTTGAGGCCGCATGCCGGCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCGGCGTTGACAGCGGTTACAGGc                                                                                                                                                                                          <  2:429293/247‑1 (MQ=255)                                                              ttGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACa                                                                                                                                                                                                      >  1:41033/1‑236 (MQ=255)                                                              ttGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCGCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACa                                                                                                                                                                                                      <  2:41033/236‑1 (MQ=255)                                                                      gCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCt                                                                                                                                                                               <  2:217138/251‑1 (MQ=255)                                                                        aGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTAt                                                                                                                                                                             <  1:376519/251‑1 (MQ=255)                                                                        aGGAAGAAGCGATCTCTGAAGCCACCCGTAAGAGGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGAGTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACGGCGTTTACAGGCTTATTTCGGCTAt                                                                                                                                                                             <  2:433377/251‑1 (MQ=255)                                                                            agaagCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCACTCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTAtctc                                                                                                                                                                          >  1:324946/1‑250 (MQ=255)                                                                                 cGATCTCTGAAGCCACCCCCAAGCTGCGCGAAACGCAAACCACGCTCAATCAACTCAATACACAGATTGATGAGATGAACGCGACGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGAGCAGCCTCGCCGCACACCTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGATGATTCTCAGCGGTGACGAAAGCCAGCGTGGACCGCGTTTCCAGGCTTATTTCGGCTATCTCaccc                                                                                                                                                                      >  2:279612/1‑246 (MQ=255)                                                                                      tctGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCg                                                                                                                                                                                                   >  2:147286/1‑215 (MQ=255)                                                                                      tctGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCg                                                                                                                                                                                                   <  1:147286/215‑1 (MQ=255)                                                                                                     aaGCTGCGCGAAACGCAAAACACGCTCAATCAACACAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCATGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGc                                                                                                                                                <  1:403331/251‑1 (MQ=255)                                                                                                      aGCTGCGCGAAACGCAAAACACGCTCAATCCACTCCATAAACAGATTGATGCGATGAACGCGTCGATTGCCAAACCGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCACCCAGGAGCGACAAGAAACCATTGcc                                                                                                                                               >  2:402142/1‑251 (MQ=255)                                                                                                          gcgcGAAACGCAAAACCCGCTCAATCAACTCAATAAACAGATTGATGAGAGGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGAGTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGATATCTCATCCAGGCGCGACAAGAAACCATTGCCCa                                                                                                                                             <  2:7296/249‑1 (MQ=255)                                                                                                              gAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGAGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGa                                                                                                                                        >  1:100093/1‑250 (MQ=255)                                                                                                                          acGCTCAATCAACTCTATAAACAGATTGATGTGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCAAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGCACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGATTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTAAAGCAAACGCGTg                                                                                                                            >  1:101944/1‑250 (MQ=255)                                                                                                                               caatcaaCTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTgaaga                                                                                                                        >  1:378521/1‑249 (MQ=255)                                                                                                                                aatcaaCTCAATAAACAGAGTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTGATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCAGTGCCCAGTTGAAGCAAACGCGTGAAGAAGt                                                                                                                     <  1:402142/251‑1 (MQ=255)                                                                                                                                    aaCTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGcc                                                                                                                 >  1:23367/1‑251 (MQ=255)                                                                                                                                      cTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCAt                                                                                                               >  1:298742/1‑251 (MQ=255)                                                                                                                                      cTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCATCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACTGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGCAGTCGCCAt                                                                                                               >  2:141324/1‑251 (MQ=255)                                                                                                                                            aaaCAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGAGTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTGGAAGCAAACGCGTGAAGAAGTCGCCATGCAGc                                                                                                          <  1:113508/250‑1 (MQ=255)                                                                                                                                              aCAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTg                                                                                                       <  2:91298/251‑1 (MQ=255)                                                                                                                                                 gATTGATGAGATGAACGCGTCGATTGCCCAACTGGAGCAGCAAAAAGCCGCCCAGAAGCGCAGCATCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTg                                                                                                    >  2:145293/1‑251 (MQ=255)                                                                                                                                                 gATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTg                                                                                                    >  1:273487/1‑251 (MQ=255)                                                                                                                                                     gatgagatgaACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACt                                                                                                >  2:332296/1‑251 (MQ=255)                                                                                                                                                      atgagatgaACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCCTGCAGCGTGCTGAACTg                                                                                               <  2:81551/251‑1 (MQ=255)                                                                                                                                                          gatgaACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAag                                                                                           <  1:396847/251‑1 (MQ=255)                                                                                                                                                            tgaACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGcc                                                                                                                 <  2:13816/227‑1 (MQ=255)                                                                                                                                                            tgaACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGcc                                                                                                                 >  1:13816/1‑227 (MQ=255)                                                                                                                                                            ggaACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTGGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGaatag                                                                                         <  2:405150/250‑4 (MQ=255)                                                                                                                                                             gaACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAG‑‑GCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGaa                                                                                       >  1:155294/1‑250 (MQ=255)                                                                                                                                                              aacgcattgatcgacAAACTGGAGAAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTCGATCCCGCATACCGGCAGGGCGAACATACCGGTACTCAGCTGATTCTCAGCGGTGAAGACAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTTTCTCAACCAGGCGCGACCAGAAACCATTGCCCAGTTGAAACAAACGCGTGAAGAAGTCGACATGCCGCGTGCAGAACTggcatgaa                                                                                        >  2:255106/15‑244 (MQ=255)                                                                                                                                                              aaCGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAgaga                                                                                        >  1:232203/1‑250 (MQ=255)                                                                                                                                                                   gTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACaaa                                                                                  <  1:260662/251‑3 (MQ=255)                                                                                                                                                                          gCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGGTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCACTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGca                                                                           <  2:273487/251‑1 (MQ=255)                                                                                                                                                                             aaaCTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGAGCTGAACTGGAAGAGAAACAAAGCGAGcaac                                                                         >  1:318573/1‑250 (MQ=255)                                                                                                                                                                                 tGGAGCAGCAAAAAACCGCCCAGGAGCGCAGCATCGCCGCACAACTGGATGCAGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAGGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACg                                                                    >  1:226925/1‑251 (MQ=255)                                                                                                                                                                                            aaaGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACaaa                                                                                  >  1:366484/1‑224 (MQ=255)                                                                                                                                                                                            aaaGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACaaa                                                                                  <  2:366484/226‑3 (MQ=255)                                                                                                                                                                                                     cAGGAGCGCAGCCACGCCGCACAACTGGATGCCGCATTCCTTCAGGGCGAACATACCGGCATTAAGCTGATTATCAGCGGTGAAGAACGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTGTCTCAACCCGACGCGACAAGAAACCATTGCACAGTTGAAGCAAACGAGTGAAGAAGTCGCCATGCAGCGTGCTGACCTGGCAGAGAAACAAACCGAGCAACACAGGCTGTTAAATGAACAgcgcgc                                                >  2:254851/1‑251 (MQ=255)                                                                                                                                                                                                      aGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGcc                                               >  1:338698/1‑251 (MQ=255)                                                                                                                                                                                                                             cTGGATGCCGCATTCCGTCAGGGCGAACATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGCCCAACAGGCGAAACTGACTCAGgc                        >  1:313201/1‑251 (MQ=255)                                                                                                                                                                                                                                                   gCGAACATACCTGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTGCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAAAGCGAGCAACAAACGCTGTTATATGAACAGCGCGTCCAACAGGCGAAACTGACTCAGGCTCTGAATGAGCGTAAAAAGACg  <  1:185295/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       CGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAGTCGCCATGCAGCGTGCTGAACTGGAAGAGAAACAGAGCGAGCAACAAACGCTGTTATATGAGCAGCGCGCCCAACAGGCGAAACTGACTCAGGCGCTGAACGAGCGTAAAAAGACG  >  NC_000913/3786978‑3787470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑