Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,786,975	C→T	D46D (GAC→GAT)	envC →	murein hydrolase activator EnvC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,786,975	0	C	T	100.0%	217.1 / NA	65	D46D (GAC→GAT)	envC	murein hydrolase activator EnvC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/34);  total (31/34)

GCCGACCATGTTGTCGCTGATGGGTATGGAAATCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTC  >  NC_000913/3786729‑3787212                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              gcCGACCATGTTGTCGCTGATGGGTATGGAAATCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATcg                                                                                                                                                                                                                                           >  2:14737/1‑251 (MQ=255)               cGCTGATGGGTATGGAAATCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAgcg                                                                                                                                                                                                                              <  1:391933/250‑1 (MQ=255)                   gATGGGTATGGAAATCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCgg                                                                                                                                                                                                                         <  2:80870/251‑1 (MQ=255)                    aTGGGTATGGAAACCCCGCAAGAGATGACGGGTAGGCCGCTGTTCATCGTAGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAAGACCATGCCACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCGTCATCGACGTCATCGTGCTTAGCTCTGGCGTAGTGTTGTGCGCGTTTTCCGCCCATGCGGATGAGTGTTACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGTGCGCGCGGt                                                                                                                                                                                                                        <  1:200199/251‑1 (MQ=255)                    aTGGGTAGGGAAATCCCGCAAGAGATGACGGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGt                                                                                                                                                                                                                        <  2:138432/251‑1 (MQ=255)                      gggTATGGAAATCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAAGCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGTCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTAc                                                                                                                                                                                                                      <  2:292046/251‑1 (MQ=255)                      gggTATGGAAAGCCCGAAAGAGAGGACTGGTAAGCCGATGTTCATCATGGAATAAGCCCTCCCCATGAGGGGAAAGGCTATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAATAGCGCGCGGTa                                                                                                                                                                                                                       <  2:17725/250‑1 (MQ=255)                          aTGGAAATCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGcc                                                                                                                                                                                                                   <  1:224347/250‑1 (MQ=255)                               aaTCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCCCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGc                                                                                                                                                                                                             <  2:121676/251‑1 (MQ=255)                                    cGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGcaacaa                                                                                                                                                                                                        <  2:315424/251‑1 (MQ=255)                                            aTGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACgcgc                                                                                                                                                                                                <  1:136670/251‑1 (MQ=255)                                             tGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATcgccg                                                                                                                                                                                                                                        <  2:94382/210‑1 (MQ=255)                                             tGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATcgccg                                                                                                                                                                                                                                        >  1:94382/1‑210 (MQ=255)                                                      aGCCGCTGTTCATCGTGGAATAATCCCGCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGGAATCAGGCCCATCATCTACGCCAGCGTTCGTAGCGCTGGCGTATGGTTGTGCGCCTTGTCCGCCCATACGGATGAGCGTGACCAAGTCAAAACTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTc                                                                                                                                                                                      <  1:59313/251‑1 (MQ=255)                                                       gCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCg                                                                                                                                                                                     <  2:374610/251‑1 (MQ=255)                                                          gCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGGGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCACCAACAACGCGCAAGCCTGCTCGccc                                                                                                                                                                                  >  2:370714/1‑249 (MQ=255)                                                           cTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCGGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCTCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCCGGACGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAAAAACCCGCAAGCCTGCGCGcac                                                                                                                                                                                  >  2:299451/1‑250 (MQ=255)                                                            tGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGcaca                                                                                                                                                                                 >  1:386754/1‑250 (MQ=255)                                                             gTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCTCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAAt                                                                                                                                                                               <  1:374059/251‑1 (MQ=255)                                                                cATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTg                                                                                                                                                                             <  2:102222/250‑1 (MQ=255)                                                                 aTCGTGGACTAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCAGGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGa                                                                                                                                                                            >  1:333125/1‑250 (MQ=255)                                                                                ctcccCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGgaa                                                                                                                                                                 >  1:173243/1‑246 (MQ=255)                                                                                ctcccCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGc                                                                                                                                                            >  2:396847/1‑251 (MQ=255)                                                                                ctcccCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGGGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGgaa                                                                                                                                                                 <  2:173243/246‑1 (MQ=255)                                                                                   cccATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAAGCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGAt                                                                                                                                                         <  1:132525/251‑1 (MQ=255)                                                                                    ccATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGCTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCgctc                                                                                                                                                        >  2:94086/1‑248 (MQ=255)                                                                                            ggAAAGGCGATTAATACCATGACACGGGCCGTGTAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCACCCATGCGGATGAGCGTGTCCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACACCACACGCAACAACAACGCGCAAGCCTGCTCGTACAATTGAAACAGCAGGACGAAACGAtctatggagc                                                                                                                                                >  2:92776/1‑244 (MQ=255)                                                                                                               tGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTCGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGGGACCAACTCAAATCTATTAAGGCCGATATCGCCGCGAACGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAGTTGAAAAAGCAGGAAGAAGCGATCTCTGAAGGCACCCGAAAGcagagcga                                                                                                                              >  2:113508/1‑243 (MQ=255)                                                                                                                  cacGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGAtc                                                                                                                                                        >  2:9174/1‑221 (MQ=255)                                                                                                                  cacGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGAtc                                                                                                                                                        <  1:9174/221‑1 (MQ=255)                                                                                                                     gggCCGTGAAACCGCGCAGGTTTGCCATCAGGACCATCATCTACGCCAGCGTTCTTAACGCTGGCGTATTGTTGTGCGCCATTTCCGCCCATGCGGATGAGAGTGACCCACTCAAATCTATTCAGGCCGATATCGCAGCGAACGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGACTGCTCGCACAATTGAAACCGCAGGAAGAAGCGATCTCTGAAGCCAACCGTAAGCTGCGCAAAACGCa                                                                                                                        >  2:407864/1‑250 (MQ=255)                                                                                                                      ggCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGATCGCACAATTGAAAAAGCAGGAAGAAGCGCTCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCaa                                                                                                                       >  1:193804/1‑250 (MQ=255)                                                                                                                        ccGTGAAACCTCGCAGGTGTGCAAGCAGGCCCATCATCTACGCCAGCGTTCCTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGTCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAac                                                                                                                    <  1:183375/251‑1 (MQ=255)                                                                                                                          gTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAacac                                                                                                                  >  1:41286/1‑251 (MQ=255)                                                                                                                                ccGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCtcaa                                                                                                            <  1:14737/251‑1 (MQ=255)                                                                                                                                 cgcgCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCtcaat                                                                                                           <  1:170884/251‑1 (MQ=255)                                                                                                                                     cAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAAc                                                                                                       <  1:324395/251‑1 (MQ=255)                                                                                                                                             cAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGCTATCGCCGCGAAAGAGCGCGCGGTACGCCAACAGCAACAACAACGCGCAAGCCTGCTCGCACCATTGAAAAAGCAGGACGAAGc                                                                                                                                                            >  2:72917/1‑190 (MQ=255)                                                                                                                                             cAATCAGGCCCATCATCTACGCCAGCGTTCTGAGCGCTGGCGTAGTGTTGTGCGCCTTTGCCGCCCATGCGGATGAGCGTGACCAACGCAAACCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGc                                                                                                                                                            <  1:72917/190‑1 (MQ=255)                                                                                                                                                           atcTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGCAGCCACCCTTAAGCTGCGCGCAACGCAAACCACGCTCAAGACACTCAATAAACAGATtgatg                                                                                     >  2:416484/1‑247 (MQ=255)                                                                                                                                                               aCGCCAGCGTTCTTAGCGCTGGCGTATTGGTGTGCGCCTTTTCCTCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTgatgagatga                                                                               <  1:215227/249‑1 (MQ=255)                                                                                                                                                                 gCCAGCGTTCTTAGCGCTGGCGTATTGGTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAAcgc                                                                           <  2:41286/251‑1 (MQ=255)                                                                                                                                                                     gCGTTCTTAGCGCTGTCGTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAATACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCg                                                                       <  1:213902/251‑1 (MQ=255)                                                                                                                                                                       gTTCTTAGCGCTGGCGTATTGTTGTGAGCCGTTTCCGCCCATGCGGATGAGCGGGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAGCACGCTCAATCAACTCAATAATCAGATTGATGAGATGAACGCGTCGAt                                                                     <  2:43035/251‑1 (MQ=255)                                                                                                                                                                        ttCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGAtt                                                                    >  2:354771/1‑251 (MQ=255)                                                                                                                                                                            tAGCGCTGGCTTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGc                                                                  <  1:203756/249‑1 (MQ=255)                                                                                                                                                                               cgcTGGCGTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGCACGCGTCGATTGCCAAAc                                                             >  1:99533/1‑251 (MQ=255)                                                                                                                                                                               cgcTGGCGTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAAc                                                             >  2:13138/1‑251 (MQ=255)                                                                                                                                                                                 cTGGCGAATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAAGCCGATATCGCCGCGACAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACACAACACACGCTCAATCAACTCAATAAACAGATTAATGAGATGAAcgcagcgaatgcaagcctg                                                           >  1:357750/1‑235 (MQ=255)                                                                                                                                                                                   ggCGTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGTTGAGATGAACGCGTCGATTGCCAAAcggg                                                          >  2:272163/1‑247 (MQ=255)                                                                                                                                                                                     cGTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACACCAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGagc                                                       >  1:68920/1‑251 (MQ=255)                                                                                                                                                                                      gTATTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGagc                                                       >  1:408784/1‑250 (MQ=255)                                                                                                                                                                                        aTTGTTGTGCGCCTTTTCCGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGagcagc                                                    >  1:146004/1‑251 (MQ=255)                                                                                                                                                                                                      tttCCGCCCATGCGGATAAGCGTGACCAACACAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGAGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCATGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACTCTCAATCAACTCAATAAACAAATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAgccgcct                                        <  2:14253/249‑2 (MQ=255)                                                                                                                                                                                                         ccGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGATCAGCAAAAAGCCGCCCAGGa                                    <  2:416327/250‑1 (MQ=255)                                                                                                                                                                                                         ccGCCCATGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAg                                   <  2:146004/251‑1 (MQ=255)                                                                                                                                                                                                             ccatgcgGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAgcgcc                               >  1:74732/5‑250 (MQ=255)                                                                                                                                                                                                             ccatgcgGATGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCa                               <  1:298598/247‑1 (MQ=255)                                                                                                                                                                                                             ccatgcgGATGAGCGTGAACAACTCACATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACACTTGCAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAgcgc                                >  2:185236/5‑250 (MQ=255)                                                                                                                                                                                                                     aTGAGCGTGACCAACTCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGACGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTAAATCAACTCCATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTcgcc                       >  1:67615/1‑251 (MQ=255)                                                                                                                                                                                                                         gCGTGCCCAACTCAAATCTATTCCGGCAGATATCGCCGCGAAAAAGCGCGCGGTACGCAAAAAGCAACAACAACGCGAAAGCCTGCTCGCACAATTGAAAACGCAGGAAGAAGCCATCTCTGAAGCCCCCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACACAAAAAACAGATTGCTGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCACAGGAGCGCAGCCTCGCCGcac                   >  2:185295/1‑251 (MQ=255)                                                                                                                                                                                                                                    tCAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGAGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGcc        >  2:344913/1‑251 (MQ=255)                                                                                                                                                                                                                                     cAAATCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCACAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATgtc        <  2:386754/250‑3 (MQ=255)                                                                                                                                                                                                                                         tCTATTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCAtt   >  1:91298/1‑251 (MQ=255)                                                                                                                                                                                                                                            aTTCAGGCCGATATCGCCGCGAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTc  <  1:332185/249‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              GCCGACCATGTTGTCGCTGATGGGTATGGAAATCCCGCAAGAGATGACTGGTAAGCCGCTGTTCATCGTGGAATAATCCCTCCCCATGAGGGGAAAGGCGATTAATACCATGACACGGGCCGTGAAACCGCGCAGGTTTGCAATCAGGCCCATCATCTACGCCAGCGTTCTTAGCGCTGGCGTATTGTTGTGCGCCTTTTCCGCCCACGCGGATGAGCGTGACCAACTCAAATCTATTCAGGCTGACATCGCCGCAAAAGAGCGCGCGGTACGCCAAAAGCAACAACAACGCGCAAGCCTGCTCGCACAATTGAAAAAGCAGGAAGAAGCGATCTCTGAAGCCACCCGTAAGCTGCGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTC  >  NC_000913/3786729‑3787212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑