Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,786,519 G→A A420A (GCG→GCA)  gpmM → 2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,786,5190GA97.7% 111.4 / NA 44A420A (GCG→GCA) gpmM2,3‑bisphosphoglycerate‑independent phosphoglycerate mutase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base A (19/23);  minor base C (1/0);  total (21/23)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

ACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGC‑GAAAGCGGTTG‑AGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGG‑TA‑ACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGTTGAAGGCGGCAAACTTTCTGACATCGCGCCGACCATGTTGTCGCTGATGGGTATGGAAATCC  >  NC_000913/3786275‑3786763
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                        
aCGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGTTGAAGCACTGGAt                                                                                                                                                                                                                                                    <  1:383157/251‑1 (MQ=255)
     aCTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATGATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGTTGAAGCACTGGATCACTg                                                                                                                                                                                                                                               <  1:286466/251‑1 (MQ=255)
      cTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGTTGAAGCACTg                                                                                                                                                                                                                                                       <  2:39692/242‑1 (MQ=255)
      cTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGGTGAAGCACTgagatcggg                                                                                                                                                                                                                                               >  1:39692/1‑242 (MQ=255)
       tttCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAA‑CGGGTGAAGCACTGGAACACcgcg                                                                                                                                                                                                                                             >  1:249538/1‑251 (MQ=255)
            tcttcAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGTTGAAGCACTGGATCACTGCGTg                                                                                                                                                                                                                                           <  1:413721/248‑1 (MQ=255)
             cttcAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGGTAAAG‑AGGGTGAAGCGCttgaactatgcgtggg                                                                                                                                                                                                                                         >  2:187612/1‑234 (MQ=255)
                        gCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGGTGGAGGACTTGGTCACCGCGTGGAAGAAGTCGG‑Gaa                                                                                                                                                                                                                            >  1:83782/1‑250 (MQ=255)
                          gTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGTTGAAGCACTGGATCACTGCGGTGAAGAAGTCGC‑GAAAGc                                                                                                                                                                                                                         <  1:132391/251‑1 (MQ=255)
                                           aGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑AGGGTGGAGCACTGGATCACTTCGTGGGAAAAGTCGC‑GAAAGAGGATG‑AGTCCgtggggg                                                                                                                                                                                                       >  1:431999/1‑249 (MQ=255)
                                                                cAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCGCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACAACGGGGTGATGGAAGCGGCGGTTAAAG‑CGTTTGAAGCACTGGGTCACTGCGTGGAAGAATTCGC‑GAAAGCGGTTG‑AGTCCGTGGGTGGACAACTGCTGATCACCGCt                                                                                                                                                                                   <  2:430556/250‑1 (MQ=255)
                                                                  aCTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGGTAAAG‑CGGTTGGAACACTGGGTCACTGCGGGGAAGAAGACGC‑GAAAGCGGGTG‑GGTCCGTGGGGGGACAAATGCCGATCACCCCTgcc                                                                                                                                                                                >  1:80870/1‑249 (MQ=255)
                                                                        cGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGC‑GAAAGCGGTTG‑AGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGG‑t                                                                                                                                                                         <  2:171238/251‑1 (MQ=255)
                                                                         gAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGC‑GAAAGCGGTTG‑AGTCCGTGGGTGGACAACTGCTGATc                                                                                                                                                                                         <  1:433248/235‑1 (MQ=255)
                                                                          aaaGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGC‑GAAAGCGGTTG‑AGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGG‑Ta‑a                                                                                                                                                                      <  1:319441/251‑1 (MQ=255)
                                                                                 ctacctacGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGGTGAAGCACCGGGGCACTGCGTGGGAGAAGGCGC‑GGAAGGGGGTG‑AGGCCGGGGGGGGGCAAACGGTGATCACCGCTGGCCACGG‑GA‑ACGCTgg                                                                                                                                                                >  2:75628/1‑249 (MQ=255)
                                                                                  tacctacGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAA‑CGGTTGTAGCACTCGGTCACTCAGTGGAGAAAGTGGC‑GAAAGAGGGTGTAGTC‑GGGGGGGGGCAACAGCTGATCACCGCCGCACACGG‑TA‑ACGCTGaa                                                                                                                                                               >  2:14192/1‑249 (MQ=255)
                                                                                   acctacGATCTGCAACCGGAAATGAGCCCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGC‑GAAAGCGGTTG‑AGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGG‑TA‑ACGCTGaac                                                                                                                                                              <  1:382749/250‑3 (MQ=255)
                                                                                    cctacGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGC‑GAAAGCGGTTG‑AGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGG‑TA‑ACGCTGAACAg                                                                                                                                                            <  1:187612/251‑1 (MQ=255)
                                                                                     ctacGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAA‑CGGGTGGAGCACTGGATCACTGCGTGGACGAAGTCGC‑GCAAGCGGGTG‑AGGGCGGGGGTGGACAAATGCCGGTCCCCGCCCACCCAGG‑TA‑ACGCTGaaacg                                                                                                                                                            >  2:118169/1‑246 (MQ=255)
                                                                                                aaCCGGAAATGAGCTCGGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAAACCGACACCTTTAGCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGTGGAAGCACTGGATCACTGCGTGGAAGAAGTCGC‑GATAGCGGTTG‑AGTCCGTGGGTGGACATCTGCTGATCACCGCTGTCCACGG‑TA‑ACGCTGATCAGATGCGCGATCCg                                                                                                                                                <  2:434434/251‑1 (MQ=255)
                                                                                                                             gAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGC‑GAAAGCGGTTG‑AGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGG‑TA‑ACGCTGAACAGATGCGCGATCCGGCAACGGGTCAggcacacacggcacacac                                                                                                                   <  2:83782/251‑1 (MQ=255)
                                                                                                                                            gCCATCAAGAGCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGC‑GAAAGCGGTTG‑AGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGG‑TA‑ACGCTGAACAGAGGCGCGATCCGGCAACGGGTCAGGCACACACGTCACACACCAACCTGCCAGTTcc                                                                                                    <  2:84200/251‑1 (MQ=255)
                                                                                                                                              cATCAAGAGCGGCAAATACGACACCATTATCTGAAACTATCCGAACCGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAGAGGGTTGAA‑CACTTGATAACTGCGGGGGAACAGTGGG‑GACAGGGGTTG‑AGTCCGTGGGGGGAAAAATGATGGTTCCCGCTGAACACGG‑GA‑ACGCCGGACAGAAGCGCGCTCAGGCAAAGGGGCAGGGACACACGGCACACACCACCCCGCCAGTTCCGc                                                                                                  >  1:276338/1‑251 (MQ=255)
                                                                                                                                                   agagCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTTAAG‑CGGTTGAAGCACTGGATCACTGCGTGGGAGACGTCGC‑GCACAAGGTTG‑GGTCCGTGGGGGGACAACCGCTGATCACAGCTGAACACGG‑GACAC‑CTGCAAAGCAGCGCGGTCCCGCAACGGGGCAGGCACACACGGCACACACCACCCtacccggtcccctggtt                                                                                             >  2:391933/1‑235 (MQ=255)
                                                                                                                                                   agagCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGC‑GAAAGCGGTTG‑AGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGG‑TA‑ACGCTGAACAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGAtt                                                                                             <  2:302189/251‑1 (MQ=255)
                                                                                                                                                   agagCGGCAAATACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGGTGTAGCACTGGATCACTTCGTGGGAGAAGTCGC‑GCAAGCGGGTG‑AGTCCGTGGGTGGGGAACTGCTGGTCACCGCCGACCACGG‑GA‑ACGCTGGACAGATGGGCGGTCCGGGCACGGGGCAggcacacaccgcacacacacacccgccccttcccccgatt                                                                                             >  2:221403/1‑229 (MQ=255)
                                                                                                                                                            aaTACGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTTAAG‑CGGGTGAAGCACTGGATCACTGCGGGGGAGAAGTCGC‑GGAAGAGGTTG‑AGTCCGTGGGGGGGCAACTGCTGATCACCGCTGACCACGG‑TA‑ACGCTGGAAAGATGCGCGCTCCGGCAACGGGGCAGGCACACACGGCACCCACCAACCCGCCAGTTCCGCTGATTTTCGtttgt                                                                                    >  1:102222/1‑248 (MQ=255)
                                                                                                                                                                cGACACCATTATCTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGGTAAAG‑CGGATGTAGCCCTGGATCCCTGCGTGGGAGCAGGCGCAGAAA‑AGGGTG‑AGTACGCGGGTGGGAAAATGCTGATACCCGCTGACCACGGGTA‑A‑GCTGCACAGGTGCGCGGTCCGGCCACGGGGCCAGCACACACCGCAACCACCACCATGCCACCTCCCCTGATTTACGtgtgtggt                                                                                 >  2:224347/1‑243 (MQ=255)
                                                                                                                                                                      cattatcTGTAACTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGGTGAAGCACTGGATCACTGCGGGGGAGAAGACGC‑GGAAGCGGGTG‑AGTGCGGGGGGGGGCAACTGCTGGTCACCGCCGACCCCGG‑GA‑ACGGTGGACAGATGCGCGATCCGGCCACGGGGCAGGGACACACGGCCCACACCAAACCGCCAGTTCCGCTGGTTTACGGTGGGGATAAGaa                                                                            >  1:332335/5‑249 (MQ=255)
                                                                                                                                                                         tatcTGTAACTAGCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGC‑GAAAGCGGTTG‑AGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGG‑TA‑ACGCTGAACAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTg                                                                        <  2:332335/249‑1 (MQ=255)
                                                                                                                                                                                  cTATCCGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAA‑CGGCTGAAGCACCGGGTCAATGCGGGGGAGAAGGGGC‑GAAAACGGTGG‑AGTCCGGGGGGGGACAAATGCTGCTCACCGCTGACCACGG‑TA‑AAGCCGAACAGATGCGCGCTCCGGCAAAGGGTCCGGGACACCCGGCGCACACCCACCTCCCAGTTCCGCCGATTTTCGTTGGTGATAAGAAAGTGaaaaaggttg                                                              >  1:43035/1‑244 (MQ=255)
                                                                                                                                                                                       cGAACGGTGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGATAAAG‑CGGGTGAAGCACTGGGTAACTGCGGGGGAGAAGTCGC‑GGAAGAGGGTG‑GGTCCGGGGGGGGGCAACTGCTGATAACCGCTGGCCACGG‑GA‑AAGCTGAACAGATGCGCGATACGGCCACGGGGCCGGCACACACGGCACACCCCCACCCGCCCGTTCCCCCGGTTTAAGTTGGTGATaaaaaaggggaaacgggtgcaggg                                                         >  1:203933/1‑229 (MQ=255)
                                                                                                                                                                                                       aGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGGTGAAGAACTGGATCACTGCGTGGGAGAAGGCGC‑GGAAGCGGTTG‑AGTCCGTGGGTGGACAAATGCTGATCACCCCTGACCACGG‑TA‑ACCCTGAACAGATGCGCGGTCCGGCAACGGGGCAGGCACACAAGGCACACACCCAACTGCCAGGTCCCCTGATTTACGTTGGGGGTAAGAACGAGAAAGCGGGTGCAGGCGGGAAACTTTCttaa                                          >  1:433377/1‑247 (MQ=255)
                                                                                                                                                                                                         gTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGC‑GAAAGCGGTTG‑AGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGG‑TA‑ACGCTGAACAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGTTGCAGGCGGCAAACTTTCTGACATc                                       <  1:424103/251‑1 (MQ=255)
                                                                                                                                                                                                              acCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGC‑GAAAGCGGTTG‑AGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGG‑TA‑ACGCTGAACAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGTTGCAGGCGGCAAACTTTCTGACATCGCGcc                                  <  1:221403/251‑1 (MQ=255)
                                                                                                                                                                                                                      gATGGAAGCGGCGGTTAAAG‑CGGTTGAAGCACTGGATCACTGCGTGGGAGAAATCGC‑GAAAGGGGTTG‑AGTCCGTGGGGGGACAACTGCTGGTCACCCCCGGCCACCG‑GA‑AAGCTGGACAGATGCGGGATCCGGCAACGGGGCAGGCACACACGGCCCACACCAACCCGCCCGTTCCGCCGATTTACGGTGGGGGTAAGAACCTGAAAGCGGTTGCAGGCGGGCAACTTTTTGACATccgccccgccctgg                          >  2:353576/1‑238 (MQ=255)
                                                                                                                                                                                                                           aaGCGGCGGTTAAAG‑CGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGC‑GAAAGCGGTTG‑AGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGG‑TA‑ACGCTGAACAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGTTGCAGGCGGCAAACTTTCTGACATCGCGCCGACCATGTTGTCg                     <  1:353576/251‑1 (MQ=255)
                                                                                                                                                                                                                             gcggcgGTTAAAG‑CGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGC‑GAAAGCGGTTG‑AGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGG‑TA‑ACGCTGAACAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGTTGCAGGCGGCAAACTTTCTGACATCGCGCCGACCATGTTGTCGCt                   <  1:350135/251‑1 (MQ=255)
                                                                                                                                                                                                                               ggcggTTACAG‑CGGTTGCAGCACTGGATCACTGCGTGGCAGAAGTCGC‑GAAAGCCGTTG‑AGTCCGTGGGTGGACCAATGCTGATCACCGCTGACCCCGG‑TA‑ACGCTGAACAGATGCGCGATACGGCCACGGGTCAGGCCCACACCGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGTTGCAGGCGGCCAACTTTCTGACATCGCGCCGGCCAtgtttgcgctg                  >  2:202009/1‑243 (MQ=255)
                                                                                                                                                                                                                                     tAAAG‑CGGTTGAAGCACTGGATCACTGCGTGGAAGAAGTCGC‑GAAAGCGGTTG‑AGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGG‑TA‑ACGCTGAACAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGTTGCAGGCGGCAAACTTTCTGACATCGCGCCGACCATGTTGTCGCTGATGGGt            <  1:75628/250‑1 (MQ=255)
                                                                                                                                                                                                                                       aaG‑CGGTTGAATCACTGTATCACTGCGTGGAAGAAGTCGC‑GAAAGCGGTGG‑AGTCCGTGGGTGGACATCTGCTGATCACCGCTGACCCCGG‑TA‑ACGCTGAACATATGCGCGATCCGGCAACGGGTCAGGCACACACGTCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGTTGCAGGCGGCAAACTGTCTTACATCGCGCCGACCATGTTGTCGCTTATGGGTATg         <  2:265061/251‑1 (MQ=255)
                                                                                                                                                                                                                                               tGAAGCACTGGATCACTGCGTGGAAGAAGTCGC‑GAAAGCGGTTG‑AGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGG‑TA‑ACGCTGAACAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGTTGCAGGCGGCAAACTTTCTGACATCGCGCCGACCATGTTGTCGCTGATGGGTATGGAAATcc  >  2:324395/1‑251 (MQ=255)
                                                                                                                                                                                                                                                gAAGCACTGGATCACTGCGTGGAAGAAGTCGC‑GAAAGCGGTTG‑AGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGG‑TA‑ACGCTGAACAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGTTGCAGGCGGCAAACTTTCTGACATCGCGCCGACCATGTTGTCGCTGATGGGTATGGAAATcc  <  1:202009/250‑1 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                        
ACGTTACTTTCTTCTTCAACGGTGGCGTAGAAGAGTCGTTCAAAGGCGAAGATCGCATTCTGATCAACTCACCGAAAGTGGCTACCTACGATCTGCAACCGGAAATGAGCTCCGCAGAGCTGACCGAAAAACTGGTTGCGGCCATCAAGAGCGGCAAATACGACACCATCATCTGTAACTATCCGAACGGCGACATGGTAGGTCACACCGGGGTGATGGAAGCGGCGGTTAAAG‑CGGTTGAAGCGCTGGATCACTGCGTGGAAGAAGTCGC‑GAAAGCGGTTG‑AGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGG‑TA‑ACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGTTGAAGGCGGCAAACTTTCTGACATCGCGCCGACCATGTTGTCGCTGATGGGTATGGAAATCC  >  NC_000913/3786275‑3786763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑